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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Circ Res. Author manuscript; available in PMC 2013 September 14.
Published in final edited form as:
PMCID: PMC3766357
NIHMSID: NIHMS400407

A Nodal to TGFβ Cascade Exerts Biphasic Control Over Cardiopoiesis

Abstract

Rationale

The Transforming Growth Factor–β (TGFβ) family member Nodal promotes cardiogenesis, but the mechanism is unclear despite the relevance of TGFβ family proteins for myocardial remodeling and regeneration.

Objective

Determine the function(s) of TGFβ family members during stem cell cardiogenesis.

Methods and Results

Murine embryonic stem cells (mESCs) were engineered with a constitutively active human Type I Nodal receptor (caACVR1b) to mimic activation by Nodal and found to secrete a paracrine signal that promotes cardiogenesis. Transcriptome and gain- and loss-of-function studies identified the factor as TGFβ2. Both Nodal and TGFβ induced early cardiogenic progenitors in ESC cultures at day 0–2 of differentiation. However, Nodal expression declines by day 4 due to feedback inhibition whereas TGFβ persists. At later stages (day 4–6), TGFβ suppresses the formation of cardiomyocytes from multipotent Kdr+ progenitors, while promoting the differentiation of vascular smooth muscle and endothelial cells.

Conclusions

Nodal induces TGFβ, and both stimulate the formation of multipotent cardiovascular Kdr+ progenitors. TGFβ, however, becomes uniquely responsible for controlling subsequent lineage segregation by stimulating vascular smooth muscle and endothelial lineages and simultaneously blocking cardiomyocyte differentiation.

Keywords: Nodal, Cripto, TGFβ2, Kdr, cardiogenesis

INTRODUCTION

Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) hold great potential as sources of cardiomyocytes, and as models to understand how cardiomyocytes, vascular smooth muscle and endothelial cells arise from common cardiopoietic progenitors1. Defining the signals that control cardiopoietic differentiation will be important for numerous applications, including regenerative medicine.

The divergent Transforming Growth Factor–β (TGFβ family member Nodal is critical for the formation of the heart and other visceral organs. Nodal activates a heteromeric complex of type I [Acvr1b (Alk4), or Acvr1c (Alk7)] and type II (Acvr2a and b) serine/threonine kinase receptors, leading to phosphorylation of Smad2 and -3 that then activate target genes2. Mouse embryos lacking Acvr1b, Smad2, or Nodal, and double knockout of the two type II receptors (Acvr2a and Acvr2b) fail to gastrulate or form mesendoderm3. Genetic deletion of Cripto, an essential Nodal co-receptor in most contexts, is less severe, such that embryos form mesendoderm but are severely deficient in cardiogenic progenitor cells4, 5. The cardiogenesis deficit inherent in Cripto−/− ESCs can be rescued either by incorporation into chimeric (Cripto−/−:wildtype (WT)) embryos4, or by a constitutively active mutant human ACVR1b receptor6, demonstrating the existence of yet unknown paracrine effectors that propagate the signal from cell to cell.

We used mESCs to model cardiogenesis and found that TGFβ2 is induced by Nodal and propagates the cardiogenic signal. The essential nature of TGFβ for cardiogenesis is based on resistance to the feedback inhibitors Lefty1, Lefty2 and Cerberus1 (Cer1) that block Nodal. Consequently, both Nodal and TGFβ induce early cardiogenic progenitors, but Nodal expression declines due to feedback inhibition while TGFβ expression persists in Kdr+ cardiopoietic precursors. In this population, TGFβ suppresses cardiomyocyte differentiation, while promoting vascular smooth muscle and endothelial cell formation. Thus, a Nodal to TGFβ cascade, including feedback inhibition, provides biphasic control over cardiopoietic cell fate.

METHODS

Protocols and primer sequences are in online supplemental materials.

RESULTS

Cardiogenic rescue implicates a diffusible factor downstream of Nodal/Avcr1b

Cripto−/− mESCs are deficient in production of cardiogenic progenitors, exhibiting low Kdr and Mesp1 expression (Online Fig. IA and4, 5), and are thus ideal for a cell-mixing study to identify paracrine factors that initiate cardiogenesis downstream of Nodal/Avcr1b (Fig. 1A). A constitutively active human ACVR1b receptor (caACVR1b) was stably introduced into Cripto−/− mESCs to activate downstream signaling (Cripto−/−caACVR1b, inducers) (Online Fig. IA). Co-culture (Fig. 1A,B) of these cells dramatically restored Kdr and Mesp1 expression in eGFP-labeled Cripto−/− mESCs (responders) (Fig. 1C). Co-culture also increased the number of Kdr+ progenitors among the responder (eGFP+) population, from 3.34% ± 0.06% to 21.28% ± 1.37% after 5 days (Fig. 1D). FACS-isolated GFP+, Kdr+ cells (responders) co-expressed Mesp1 (Fig. 1E). By day 9, the induced cells expressed cardiomyocyte markers (Fig. 1F) and beat rhythmically (Online Movie I). Residual Cripto−/−caACVR1b cells contaminating the responder population after FACS (0.5%) were insufficient to account for this level of rescue (Online Fig. II). Finally, the rescue occurred cell non-autonomously, since mixtures of eGFP-labeled Cripto−/−caACVR1b inducers with Myh6-mCherry responders revealed clearly distinct patterns of eGFP and mCherry expression (Fig. 1G,H and Online Movie II).

Fig. 1
Paracrine signaling downstream of Nodal

To test if the induced Kdr+ progenitors autonomously form cardiomyocytes, aggregated responder (Cripto−/−, Myh6-mCherry, eGFP+) and inducer (Cripto−/− caACVR1b) cells were separated by FACS at day 5, re-aggregated separately, and cultured for an additional 15 days (Fig. 1I). The responder cells expressed Myh6 (Fig. 1J) and mCherry (Fig. 1K), showing that the paracrine factor(s) initiate cardiogenesis prior to day 5. Since Cripto−/− cells negligibly respond to Nodal (Online Fig. IB), the factor is neither Nodal nor a shed version of Cripto.

TGFβ2 acts downstream of Nodal to induce cardiogenic mesoderm

Microarray analysis (not shown) showed that caACVR1b upregulated mRNAs encoding TGFβ1, TGFβ2, TGFβ3 and inhibins. Of these, Tgfb2, Tγϕβ3 and Inhba were greatly upregulated by caACVR1b transfection in Cripto−/− mESCs (Fig. 2A). Since E5.5 to E7.5 mouse embryos express mRNAs encoding Tgfb2, but not Tgfb3 and Inhibins7, TGFβ2 emerged as an attractive candidate for the paracrine factor. Indeed, TGFβ2 treatment from days 0–2 gave a dose-dependent induction of genetic markers of mesoderm (Mesp1, Mixl1 and Gsc) and mesoderm derivatives (Myh6, Pecam1, Aplnr,Tagln, Cdh5 and Acta2), and the Myh6-mCherry reporter in Cripto−/− ESCs (Fig. 2B,C) and even enhanced Mesp1, Kdr and Myh6 in WT cells (Fig. 2D), revealing a functional relationship.

Fig. 2
TGFβ2 acts downstream of Nodal/Acvr1b to induce Kdr+ progenitors

To test if TGFβis necessary downstream of Nodal/Acvr1b, Cripto−/− responder ESCs were transfected with siRNA against Tgfbr1 prior to co-culture with Cripto−/−caACVR1b ESCs (Fig. 2F). Tgfbr1 siRNAs blocked induction of Kdr transcripts (to about 20% of negative control siRNA), establishing TGFβ2 as a paracrine mediator of Nodal signaling.

TGFβ2 suppresses cardiomyocyte differentiation during a late stage of differentiation

The preceding showed that TGFβ2 induces cardiogenic progenitors prior to day 5. Tgfb2 mRNA, however, continues to rise between days 4–8 (Fig. 3A) while Nodal mRNA declines, suggesting that TGFβ but not Nodal, plays a role as Kdr+ progenitors differentiate. To understand the basis for the shift from Nodal to Tgfb2, we examined expression of Lefty1, Lefty2 and Cer1, encoding Nodal inhibitors3. All three became expressed concomitantly with the decline in Nodal levels (Fig. 3A) and each was induced by Nodal/TGFβsignaling (Fig. 3B, C). Moreover, Nodal and TGFβboth induced Nodal (Figs. 2A and 3C). The fact that Cer1 and Lefty1,2 do not block TGFβ3 likely accounts for the persistence of Tgfb2 after the decline in Nodal. Interestingly, TGFβ2 does not induce Tgfb1 or Tgfb2, and only minimally induced Tgfb3 (Fig. 3C), making the cascade inherently self-limiting.

Fig. 3
Biphasic role of TGFβ2 in cardiogenesis

We next asked whether TGFβ influences cardiopoietic differentiation. siRNAs to either Tgfbr1 or Tgfbr2 transfected at day 4 unexpectedly increased expression of Myh6, as well as eGFP driven by the Myh6 promoter (Fig. 3D,E). At this time, Tgfb2 mRNA predominates in Kdr+ cells (Fig. 3F), suggesting autocrine repression of cardiomyocyte differentiation.

To gain further insight into the bimodal function of TGFβ we treated ESC cultures with SB-431542, a small molecule inhibitor of Acvr1b/1c and Tgfbr1, at early and late time windows (Figs 3G and H). Treatment between 0–2 days of culture abolished Mesp1 expression (Fig. 3G). Treatment at 4–6 days, in contrast, markedly enhanced Myh6 levels in Kdr+ derivatives (Fig. 3H). Conversely, recombinant TGFβ2 between days 4–6 suppressed Myh6 mRNA as well as Mef2c and Tbx5 protein, but increased Pecam1 and Myh11 mRNAs and the level of Pecam1 and Myh11 immunostaining (Fig. 3I–L). We conclude that a Nodal to TGFβ2 cascade enhances production of cardiogenic mesoderm prior to day 4, and that TGFβ persists to suppress cardiomyocyte differentiation of Kdr+ cells while biasing their differentiation towards endothelial and smooth muscle lineages.

DISCUSSION

Genetic and stem cell experiments have shown that Nodal acts positively and negatively in cardiogenesis depending on the developmental stage; however, the identity and function of downstream mediators were unknown4, 6, 8, 9. Our results define a Nodal to TGFβ signaling cascade that exerts positive and negative effects on progenitor induction and cardiomyocyte differentiation, respectively (Fig. 4). The biphasic function resembles that of Wnts and BMPs, both of which promote formation of cardiogenic progenitors (e.g. Mesp1+, Kdr+) during the period when mesoderm is induced, but suppress the subsequent formation of cardiac precursors (e.g. Nkx2.5+), and at least BMP acts positively again once Nkx2.5+ progenitors arise1.

Fig. 4
Summary

Mechanistically, the cascade incorporates auto-induction and inhibition properties that regulate Nodal and TGFβ expression within narrowly delimited developmental times. Nodal is well-known for activating its own transcription, as well that of its antagonists Lefty1, 2 and Cer1, yielding an auto-induction cascade that is feedback inhibited. However, TGFβ cannot auto-induce (Figs. 2A and and3C)3C) nor is inhibited by Cer1 and Lefty. Consequently, Tgfb2 expression is induced by Nodal, and persists after Nodal expression declines.

Considering the possible functions for a time-resolved Nodal-TGFβ cascade led to the finding that TGFβ suppresses cardiomyocyte differentiation while simultaneously enhancing formation of endothelial and smooth muscle lineages (Fig. 3E–L). The only other factors known to apportion cardiopoietic fate are Wnts, which also suppress cardiomyocyte differentiation at the same developmental stage1.

A specific requirement for TGFβ in cardiac differentiation has implications for understanding congenital heart defects. Genetic deletion of Tgfbr1 in mice causes severe cardiovascular defects10, and mutation of the latent TGFβ binding protein 3, which regulates TGFβ bioavailability, impairs differentiation of second heart field (SHF) cells in zebrafish11. It will be important to determine if altered TGFβ signaling at the time of cardiac progenitor specification underlies human congenital heart disease, such as the cardiac defects that can present in Loeys-Dietz syndrome caused by mutated TGFBR1 or TGFBR2.

NOVELTY AND SIGNIFICANCE

What is Known?

  • The divergent TGFβ protein Nodal is well-known to play a role in specifying cardiac tissue during early development, and is commonly used to generate cardiac cell types, including cardiomyocytes, from pluripotent stem cells.
  • The cardiogenic activity of Nodal is propagated from cell to cell by unknown paracrine signals, although a shed version of the Nodal co-receptor Cripto has been suggested to be involved.

What New Information Does This Article Contribute?

  • Nodal induces TGFβ2, and both induce the formation of cardiogenic progenitors in embryonic stem cell (ESC) cultures.
  • Nodal expression declines as cardiogenic progenitors form; TGFβ persists and suppresses cardiomyocyte differentiation while simultaneously promoting vascular smooth muscle and endothelial lineages.

TGFβ uperfamily members are important for cardiogenesis, as well as fibrosis and inflammation associated with myocardial injury. Here we describe a regulatory cascade that controls the production of TGFβ. TGFβinitially promotes the formation of multi-potent cardiac progenitors, but subsequently inhibits their differentiation to cardiomyocytes. TGFβ might play a similarly bimodal role in myocardial regeneration.

Supplementary Material

ACKNOWLEDGEMENTS

We thank Yoav Altman, Joseph Russo and Dr. Ed Monosov (SBMRI) for expert assistance.

SOURCES OF FUNDING

NIH and California Institute for Regenerative Medicine.

Non-standard Abbreviations

Acvr1b
Alk4, activin A receptor, type IB
Tgfbr1
Alk5, transforming growth factor, beta receptor I
Acvr1c
Alk7, activin A receptor, type IC
Acvr2a
activin receptor IIA
Acvr2b
type 2 Activin receptorb
Cer1
cerberus 1 homolog (Xenopus laevis)
Inhba
inhibin beta-A
Inha
Inhibin alpha
Kdr
Flk1, kinase insert domain protein receptor
Lefty1
left right determination factor 1
Lefty2
left right determination factor 2
Mesp1
mesoderm posterior 1
Myh6
αMHC, myosin, heavy polypeptide 6, cardiac muscle, alpha
Myh11
sm-MHC, myosin, heavy polypeptide 11, smooth muscle
Pecam1
CD31, platelet/endothelial cell adhesion molecule 1
qRT-PCR
quantitative real-time reverse transcription PCR
T
Bra/T, brachyury
Tgfbr2
transforming growth factor, beta receptor IIb

Footnotes

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DISCLOSURES

None.

REFERENCES

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