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Appl Microbiol. 1975 December; 30(6): 1046–1047.
PMCID: PMC376589

Improved method of selection for mutants of Pseudomonas putida.


Optimum conditions for enrichment of mutants of Pseudomonas putida in liquid culture were established using a procedure which combines N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis with an improved D-cycloserine selection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • COHEN-BAZIRE G, SISTROM WR, STANIER RY. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. [PubMed]
  • Hegeman GD. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type. J Bacteriol. 1966 Mar;91(3):1140–1154. [PMC free article] [PubMed]
  • LURIA SE, BURROUS JW. Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957 Oct;74(4):461–476. [PMC free article] [PubMed]
  • Ornston LN, Ornston MK, Chou G. Isolation of spontaneous mutant strains of Pseudomonas putida. Biochem Biophys Res Commun. 1969 Jul 7;36(1):179–184. [PubMed]

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