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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Agric Food Chem. Author manuscript; available in PMC 2013 September 4.
Published in final edited form as:
PMCID: PMC3762682

Chromatographic Profiles and Identification of New Phenolic Components of Ginkgo biloba Leaves and Selected Products


Ginkgo biloba leaves and their extracts are one of the most widely used herbal products and/or dietary supplements in the world. A systematic study of the phenolic compounds is necessary to establish quality parameters. A modified LC-DAD-ESI/MS method was used to obtain chromatographic profiles for the flavonoids and terpene lactones of Ginkgo biloba leaves. The method was used to identify 45 glycosylated flavonols and flavones, 3 flavonol aglycones, catechin, 10 biflavones, a dihydroxybenzoic acid, and 4 terpene lactones in an aqueous methanol extract of the leaves. The extracted G. biloba leaf products contained the same flavonoids as the raw leaves except for the lack of biflavones. The detected glycosylated flavonol contents were equal to or more than 0.0008% of the dry plant material. This is the first report of the presence of more than 20 of these flavonoids in G. biloba.

Keywords: Ginkgo biloba, flavonoids, terpene lactones, LC-DAD-ESI/MS identification, chromatographic flavonoid profile


The dried leaves of Ginkgo biloba L. (Ginkgoaceae) have been used as herbal remedies for centuries in China, and now their extracts are one of the most widely used herbal products and/or dietary supplements in the world (14). The flavonoids and terpene lactones (Figure 1) are considered to be the main beneficial components (5, 6). The voluntary industry standard is 24% flavonoid and 6% terpene lactones (by weight) in the powdered extracts (14). Over 30 flavonoids, including flavonol glycosides, flavonols, flavones, biflavones, and catechins, have been reported in the leaves (111). The quality of ginkgo leaves and their products and/or quantitative determination of their active components, mainly the flavonoid components and terpene lactones, have been previously reported (13, 1230). However, none have developed methods for a systematic identification of all the main phenolic components.

Figure 1
Structures of the phenolic components and terpene lactones of Ginkgo biloba leaves.

The flavonoid components far outnumber the diterpene lactones found in G. biloba leaves (11, 12, 2729). Thus, good separation and detailed identification of the indigenous flavonoid components is crucial to qualitative analysis and authentication of G. biloba leaves and their products. Chromatographic profiles are an important means of characterizing the leaf materials. For this purpose, a method was developed based on liquid chromatography with tandem diode array and mass spectrometric detection (LC-DAD-ESI/MS) to profile the flavonoids of G. biloba leaves and its products.

Materials and Methods

Plant Materials

Six G. biloba leaf samples and three G. biloba processed products (powdered extracts) were used in these studies. Leaf sample 1 was a standard reference material (SRM) 3246, G. biloba leaf, from the National Institute of Standards and Technology (NIST, Gaithersburg, MD, USA). Sample 2 was an American Herbal Pharmacopoeia (AHP) verified Botanical Reference Standard BRS-GB. Leaf samples 3 and 4 were picked from mature G. biloba trees in Columbia, MD and Kunmin, Yunnan Province, China, respectively. Samples 5 and 6 were picked from female (fruit bearing) and male (nonfruit bearing) G. biloba trees growing in Wanzhou, Chongqing City, China, respectively. The dried leaves were finely powdered and passed through 20 mesh sieves prior to the extraction experiments.2

Processed product sample 1 is SRM #3247, powdered G. biloba extract (NIST). The other two product samples, products 2 and 3, were purchased from local stores in the greater Washington, DC area.

Reagents, Solvent, and Standards

HPLC grade methanol, acetonitrile, formic acid, acetic acid, and NaOH were purchased from VWR International, Inc. (Clarksburg, MD, USA). HPLC water was prepared from distilled water using a Milli-Q system (Millipore Laboratory, Bedford, MA, USA).

Quercetin dihydrate (≥98%), rutin trihydrate (≥95%), kaempferol (≥90%), luteolin, apigenin (95%), catechin (95%), 3,4-dihydroxybenzoic acid, bilobalide, and ginkgolide A, B, and C were obtained from Sigma Chemical Co. (Saint Louis, MO, USA). Quercetin 3-O-glucoside, quercetin 3-O-rhamnoside, myricetin 3-O-glucoside, kaempferol 3-O-glucoside, kaempferol 3-O-rutinoside, isorhamnetin 3-O-rutinoside, isorhamnetin, myricetin, and syringetin were purchased from Extrasynthese (Genay, Cedex, France).

Patuletin 3-O-rutinoside was isolated from Echinacea angustifolia leaves and identified by NMR analysis (31). Patuletin was obtained from the acid hydrolysis of patuletin 3-O-rutinoside in this laboratory. Gingkolide J was a gift from Professor Zhu, Shanghai Institute of Mataria Medica, Chinese Academy of Sciences, Shanghai, China.

Extraction Method

Dried ground leaves (125 mg) were extracted with 5.0 mL of methanol–water (60:40, v/v) using sonication with a FS30 Ultrasonic sonicator (40 KHz, 100 W) (Fisher Scientific, Pittsburgh, PA, USA) for 60 min at room temperature (<35 °C at the end). Powdered leaf products (5 mg) were extracted with 5.0 mL of methanol–water (60:40, v/v). The extracts were filtered through a 0.45 μm Nylon Acrodisk 13 filter (Gelman, Ann Arbor, MI, USA). A 50 μL aliquot of the extracts was injected onto the analytical column for analysis. The analysis was completed less than 24 h after the extracts were prepared.

Acid Hydrolysis of Extracts

Filtered extracts (0.50 mL) were mixed with concentrated HCl (37%, 0.1 mL) and heated in a capped tube at 85 °C for 2 h. Then, 0.40 mL of methanol was added to the mixture, and the solution was sonicated for 10 min. The solution was refiltered prior to HPLC injection (32).

Alkali Hydrolysis of Extracts

The filtered extract (1.00 mL) was dried, and the residue was mixed with 0.30 mL of 4 M NaOH and kept at room temperature under a N2 atmosphere for 18 h. Then, 0.15 mL of HCl (37%) was added to the reaction mixture to bring the pH to approximately 1.0, and 0.55 mL of MeOH was added. The solution was refiltered prior to HPLC injection (32).

LC-DAD and ESI-MSD Conditions

The LC-DAD-ESI/MS system used has been previously described. It consisted of a quaternary pump with a vacuum degasser, a thermostatted column compartment, an autosampler, a diode array detector, and a quadrupole mass spectrometer from Agilent Technologies (Palo Alto, CA, USA). A 250 × 4.6 mm i.d., 5 μm Symmetry C18 column with a 20 × 3.9 mm i.d., 5 μm sentry guard column (Waters Corp., Milford, MA, USA) was used at a flow rate of 1.0 mL/min. The column oven temperature was set at 25 °C. The mobile phase consisted of a combination of A (0.1% formic acid in water) and B (0.1% formic acid in acetonitrile). The gradient increased linearly from 10% to 26% B (v/v) at 40 min, to 65% B at 70 min, and to 100% B at 100 min and was held at 100% B to 105 min. The last step, increasing from 65% B at 70 min to 100% B at 100 min, was a modification necessary for elution of the biflavones. The DAD was set at 270, 330, and 350 nm for real time monitoring of the peak intensities. UV spectra were recorded from 190–650 nm and were available after completion of the chromatographic run for plant component identification. Mass spectra were simultaneously acquired using electrospray ionization in the positive and negative ionization (PI and NI) modes at low and high fragmentation voltages (100 and 250 V) over the range of m/z 100–2000. A drying nitrogen gas flow of 13 L/min and temperature of 350 °C, a nebulizer pressure of 50 psi, and capillary voltages of 4000 V for PI and 3500 V for NI were used. The LC system was directly coupled to the MSD without stream splitting (32).

In order to detect less intense peaks, in the presence of more intense peaks, and to provide more sensitivity, positive and negative selective ion monitoring (PI/NI SIM) was used. For detection of all the flavones, ions at m/z 271/269 (to detect all the trihydroxyflavones), 285/283 (dihydroxy-methoxyflavone), 285/287 (tetrahydroxyflavone), 301/299 (trihydroxy-methoxyflavone), 303/301 (pentahydroxyflavone), 317/315 (tetrahydroxy-methoxyflavone), 319/317 (hexahydroxyflavone), 333/331 (pentahydroxy-methoxyflavone), and 347/345 (tetrahydroxydimethoxyflavone) were monitored. For the terpene lactones, ions at m/z 327/325 (bilobalide), 409/407 (ginkgolide A), 425/423 (ginkgolides B and J), and 441/439 (ginkgolide C) (11, 12, 2729) were monitored.

Results and Discussion

Identification of Ginkgo Leaf Flavonoids and Phenolic Compounds

UV chromatograms for the extract (270 and 350 nm) and alkali hydrolyzed extract (310 nm) of sample 1 are shown in Figure 2A–C. The NI SIM chromatograms for the extract (masses for flavones and terpene lactones) and the acid hydrolyzed extract (masses for flavones) for sample 1 are shown in Figure 2D–F. The structures of some ginkgo phenolic components and their terpene lactones are shown in Figure 1. The retention times (tR), wavelengths of maximum absorbance (λmax), protonated/deprotonated molecules ([M + H]+/[M − H]), major PI fragment ions, and PI/NI aglycone ions ([A + H]+/[A − H]) are listed in Table 1. Peak identification was based on the data in Table 1, standards, and published literature.

Figure 2
LC Chromatograms of ginkgo extracts of leaf sample 1: (A) extract, 350 nm; (B) extract, 270 nm; (C) alkali hydrolyzed extract, 310 nm; (D) acid hydrolyzed extract, SIM; (E) untreated extract, SIM; and (F) untreated extract, SIM. See text for SIM masses ...
Table 1
Peak Assignment for Aqueous Methanol Extracts of Ginkgo biloba Leaves

The identification process can be illustrated by the identification of peak 7 in Figure 3. The [M + H]+/[M − H] ions at m/z 757/755, [A + H]+/[A − H] ions at m/z 303/301, and PI fragment ions at 611 (loss of the third glycosyl, a rhamnosyl, from the glycoside) and 465 (loss of the secondary glycosyl, a rhamnosyl, from the produced fragment) (Table 1) suggested the peak was quercetin-dirhamnosylhexoside. Its UV data (λmax 256, 266 sh, and 354 nm) and retention time (tR 19.27 min) strongly suggested that the glycoside was located at the 3-position of the aglycone (30, 31). This flavonoid was finally identified as quercetin 3-O-2″,6″-dirhamnosylglucoside since this compound has been previously reported in G. biloba leaves (13) and is the only detected flavonoid that matches the listed retention time and UV and mass spectrometric data of peak 7. In the same manner, peak 12 (λmax 256, 266 sh, and 354 nm, and PI ions at m/z 771, 625, 479, and 317) was identified as isorhamnetin 3-O-2″,6″-dirhamnosylglucoside and peak 11 (λmax 266 and 348 nm, PI ions at m/z 741, 595, 449, 287) was identified as kaempferol 3-O-2″,6″-dirhamnosylglucoside. Both flavonoids have been previously reported in G. biloba leaves (13, 5).

Figure 3
Mass spectrum of quercetin 3-O-2″,6″-dirhamnosylglucoside (peak 7 in Figure 2A), obtained with positive ionization and 100 V fragmentation energy.

Peak 3 had protonated molecular and aglycone ions at m/z 773 and 303 suggesting it was a triglycoside like peak 7. Its retention time (tR 10.46 min), however, was much shorter than that of peak 7 (tR 19.27 min), suggesting that the compound might have a sugar at the 3-position and a second at one of the remaining positions (7, 5, 4′, or 3′). The UV spectra suggested that the sugars were located at the 3 and 7 positions of quercetin (32, 33). This quercetin O-triglycoside lost its first glycosyl, a rhamnosyl of the second sugar at the 3- or 7-position, to form the fragment m/z 627 (i.e., 773 – 627 = 146). Then, the diglycoside lost its first hexosyl at the 3- position to form the fragment at m/z 465 (i.e., 627 – 465 = 162). Finally, the molecule lost its third glycosyl, the hexosyl at the 7-position, to form its aglycone, m/z 303 (i.e., 465 – 303 = 162) (32, 34). This compound was tentatively identified as quercetin 3-O-rhamnosylglycoside-7-O-glucoside or its isomer quercetin 3-O-glucoside-7-O-rhamnosylglycoside since without a direct standard comparison they cannot be distinguished by LC-MS. Similarly, on the basis of retention time, UV, and mass spectrometric data listed in Table 1, peaks 5 and 6 were identified as kaempferol 3-O-rhamnosylglycoside-7-O-glucoside and isorhamnetin 3-O-rhamnosylglycoside-7-O-glucoside, or their isomers, respectively. These three glycosides (peaks 3, 5, and 6) were formed from the same saccharides connected to the same positions of the three different flavonols and have not been previously reported in G. biloba leaves.

There are two pairs of flavonol 3-O-diglycosides consisting of one rhamnoside and one glucoside, i.e., two quercetin 3-O-diglycosides (peaks 14 and 19) and two kaempferol 3-O-diglycosides (peaks 20 and 26). They all have the same UV spectra and the same molecular and aglycone ions, but the fragments for loss of the second sugar are different. Peak 14 has a fragment m/z 465 for the loss of rhamnosyl, indicating that it is quercetin 3-O-rhamnosylglucoside, while peak 26 has a fragment m/z 433 for the loss of glucosyl, indicating it is kaempferol 3-O-glucosylrhamnoside (not shown). By direct comparison with standards and references to the literature on G. biloba flavonoids (13, 611), these four peaks were further identified as quercetin 3-O-6″-rhamnosylglucoside (rutin) (peak 14), quercetin 3-O-2″-glucosylrhamnoside (peak 19), kaempferol 3-O-6″-rhamnosylglucoside (peak 20), and kaempferol 3-O-2″-glucosylrhamnoside (peak 26).

It was found that using the LC conditions of this study, rutin (peak 14) eluted nearly 2 min earlier than quercetin 3-O-glucoside (peak 17) and 4 min earlier than quercetin 3-O-2″-glucosylrhamnoside (peak 19). The pair of kaempferol diglycosides (peaks 20 and 26) also showed the same elution order (30.06 and 34.71 min). Using this elution order, a pair of isorhamnetin diglycosides (peaks 21A, 31.03 min and 28A, 36.90 min) were identified, and an additional isorhamnetin 3-O-rhamnosylglucoside (peak 21B, 31.35 min) was detected, which is most likely isorhamnetin 3-O-2″-rhamnosylglucoside. This identification was based on the fact that the flavonol 3-O-2″-rhanomosylglucoside (i.e., 3-O-hesperidoside) elutes slightly later than its isomer 3-O-6″-rhamnosylglucoside (i.e., 3-O-rutinoside) under these LC conditions (32).

The remaining nonacylated glycosylated flavones were identified as myricetin 3-O-rutinoside (peak 8), myricetin 3-O-glucoside (peak 9), patuletin 3-O-rutinoside (peak 15), patuletin 3-O-hesperidoside (peak 16), kaemperol 3-O-glucoside (peak 22), quercetin 3-O-rhamnoside (peak 23), isorhamnetin 3-O-glucoside (peak 24A), syringetin 3-O-2″-glucosylrhamnoside (peak 24B), apigenin 7-O-glucoside (peak 25), and luteolin 3′-O-glucoside or kaempferol O-hexoside (peak 29). Some of the identifications were further confirmed with standards as indicated in Table 1. Of these compounds, two patuletin 3-O-diglycosides, isorhamnetin 3-O-hesperidoside, myricetin 3-O-glucoside, and syringetin 3-O-2″-glucosylrhamnoside have not been previously reported in G. biloba leaves.

Twenty-one acylated flavone glycosides were detected and 20 of them (except peak 18) were identified as listed in Table 1. The main characteristics of the p-coumaroylglycosylated flavonols are the shift of absorption band II to 312–316 nm and PI/NI molecular ions 146 amu larger than their parent glycosides. Thus, peaks 31 and 33 were identified as quercetin 3-O-2″-(6″-p-coumaroylglucosyl)rhamnosides and kaempferol 3-O-2″-(6″-p-coumaroylglucosyl)rhamnosides. They have been previously reported (14, 11).

Peak 13 (24.78 min, [M + H]+/[M − H] at m/z 903/901, [A + H]+/[A − H] 303/301) is either kaempferol 3-O-2″-(6″-[4-glucosyl-p-coumaroy]glucosylrhamnoside) or kaempferol 3-O-6″-rhamnosyl-2″-(6″-p-coumaroylglucosyl) glucoside. The former has been reported previously (13), and the latter may be more prevalent since kaempferol 3-O-2″-glucosyl-6″-rhamnosyl-glucoside (peak AH-1 in Figure 2C, and Table 1) was detected in the alkaline hydrolyzed extract. This compound has its tR = 16.20 min, λmax 262, 342 nm, [M + H]+/[M − H] at m/z 757/755, PI fragments at m/z 595 (from the loss of the third glycosyl, a hexosyl), m/z 449 (from the loss of the second glycosyl, a rhamnosyl), and [A + H]+ at m/z 287 (from the loss of the first glycosyl, a hexosyl). The assignment of the glucosyl at the 2″ position and the rhamnosyl at the 6″-position was based on the fact that the glycosyl at 2″ position is removed first at this LC-MS condition when both positions are glycosylated (32).

Peak 10 (21.64 min, [M + H]+/[M − H] at m/z 919/917, [A + H]+/[A − H] 303/301) and its molecular and aglycone ions were 16 amu more than those of peak 13, suggesting a quercetin glycoside with the same saccharide. Considering the biosynthetic passways for peaks 13 and 10, peak 10 is most likely quercetin 3-O-6″-(p-4-glucosylcoumaroyl)-2″-glucosylrhamnoside rather than its isomer quercetin 3-O-6″-rhamnosyl-2″-(6″-p-coumaroylglucosyl)glucoside. If so, neither compounds has been previously reported in ginkgo leaves (13, 5, 11).

Nine of the remaining 16 peaks were identified as kaempferol 3-O-p-coumaroyldigylcosides and seven as quercetin 3-O-p-coumaroyldigylcosides. The diglycosides consisted of one glucoside and one rhamnoside. The sugar sequence and the position for p-coumaroyl or other acyls could not be determined by MS or MSn. Some of these peaks did not show the UV data described above, but they all disappeared following alkaline hydrolysis with the accompanying appearance of p-coumaric acid (AH-2 in Figure 2C) and a significant enhancement of peak 26 (kaempferol 3-O-2″-glucosyl-6″-rhanoside).

So far, only the 3-O-2″-(6″-p-coumaroyl)glucosylrhamnosides of kaempferol, quercetin, and isorhamnetin, and 7-O-rhamoside-3-O-2″-(6″-feruloyl)glucosylrhamnosides, 7-O-glucoside-3-O-2″-(6″-p-coumaroyl)glucosylrhamnosides, and 3-O-2″-(6″-[4-glucosyl-p-coumaroy]glucosyl)rhamnoside of kaempferol and quercetin, have been previously isolated and detected from ginkgo leaves (13, 511). Therefore, more than half of the acylated flavonol glycosides have not been previously reported in G. biloba leaves.

Nine flavonol and flavone aglycones, i.e., quercetin, kaempferol, myricetin, apigenin, 4′-O-methylapigenin (acacetin), luteolin, 3′-O-methylquercetin (isorhamnetin), 4′-O-methylquercetin (tamarixetin), and 3′,5′-O-dimethylmyricetin (syringetin), have been reported in G. biloba leaves (13, 5, 11), but only three of them, myricetin (peak 28B), quercetin (peak 43A), and kaempferol (peak 48), were observable in this study using UV or MS (total ion counts) detection. With SIM detection, 14 naturally occurring aglycones were observed in the leaf extracts (NI SIM in Figure 2E; PI SIM not shown) and the acid hydrolyzed extract (Figure 2D). Among them, peaks A-5 (quercetin) and A-8 (kaempferol) were considerably larger than others. The identification of myricetin (A-1), patuletin (A-3), luteolin (A-4), apigenin (A-6), syringetin (A-7), and isorhamnetin (A-9) was confirmed with standards, but the remaining peaks (A-10 through A-14) could only be identified as polyhydroxyflavones or as the mono- or dimethyl derivatives. Their specific structures could not be determined. The amplitude of peaks A-1, A-3, and A-5 through A-10 were relatively higher in the acid hydrolyzed extract (Figure 2D), indicating that their glycosides were present in the leaf extract (Figure 2E) and contributed to the aglycone concentration following acid hydrolysis.

Peaks 2 and 4 (clearly shown in Figure 2B) were identified as 3,4-dihydroxybenzoic acid (protocatechuic acid) and catechin, respectively. Peak 1 and several other small peaks (not numbered) in the 5–20 min and 80–100 min region (Figure 2A and B and 9) could not be identified at this stage since their UV and/or mass data were not sufficiently intense to provide positive identification.

The detection (UV and MS TIC) limits of this method (32), using the extract of 125 mg dried leaves in 5.00 mL of the solvent and 50 μL of injection volume, all of the detected glycosylated flavonols will have their content equal to or over 0.0008% dry plant material by weight.

Identification of Ginkgo Biflavones

Biflavones are compounds distinctive to G. biloba leaves and, in some cases, constitute a major fraction of the flavonoid components. So far, six biflavones and some of their glycosides have been reported (13, 5, 6, 9, 11). In this study, 10 biflavones were detected in the unprocessed leaf samples. Peaks 49, 51, 54, 55, 56, and 57, the relatively major biflavone peaks, were identified as amentoflavone, bilobetin, sequoiaflavone, ginkgetin, isoginkgetin, and sciadopitysin, on the basis of the fact that all of them were previously reported as major Ginkgo biflavones, and their UV and mass data (Table 1) matched previously published values, including the elution order on reverse phase columns (13, 6). Similarly, on the basis of the similarity of the retention times, UV spectra, and masses, the remaining 4 minor compounds (peaks 47, 50, 52, and 53) were tentatively identified as the isomers of some of Gingko major biflavones. Unfortunately, their exact structures could not be determined from the MS data, and none of them have been previously reported in Ginkgo leaves.

No significant amounts of biflavones were detected in the G. biloba processed products. The commercial extraction process appears to leave the biflavones behind. The relatively nonpolar biflavones are not efficiently extracted by the polar solvents used by most commercial companies. The biflavones are reported to be COX-2 inhibitors with the potential to serve as unique anti-inflammatory agents (35).

Detection of Ginkgo Terpene Lactones

Five G. biloba terpene lacotones were identified in the extracts by direct standard comparison. Since these compounds lack the conjugated bonds of the flavonoids, they are undetectable by UV absorption (11, 12, 2729) and can only be detected by the mass spectrometric data from the MS TIC chromatograms, specifically the [M + H]+/[M − H] and [2M − H] ions. To obtain their accurate retention times, SIM detection at m/z 327/325 showed the existence of bilobalide C (BB in Figures 2F and and6),6), which coeluted with quercetin 3-O-glucoside (peak 17). Similarly, monitoring [M + H]+/[M − H] and [2M − H] ions at m/z 441/439 and 879, m/z 409/407 and 815, and m/z 425/423 and 847 allowed the identification of ginkgolides A, B, and C (GA, GB, and GC in Figures 2F and and4).4). SIM detection permitted identification of these compounds even though they coeluted with flavonol glycosides (ginkgolide C coeluted with peak 18, and ginkgolides A and B coeluted with peak 35).

Figure 4
Mass spectra of ginkgo terpene lactones, obtained with negative ionization and 100 V fragmentation energy: (A) bilobalide (BB), (B) ginkolides A (GA) and B (GB), (C) ginkolide C (GC), and (D) ginkolide J (GJ). Their retention times were recorded from ...
Figure 6
LC Chromatograms of Ginkgo biloba raw leaf and processed product materials: (A) leaf sample-1, SRM 3246; (B) product-1 from leaf sample-1; (C) product-2; and (D) product-3.

It is worth mentioning that using the chromatographic conditions described for this study, the [2M − H] ions for ginkgolides A, B, and C, three major ginkgo terpene lactones in the extract, could be observed while that for bilobalide, another major ginkgo terpene lactone with different structure (Figure 1), was not detected. Ginkgolide J (GJ) is barely discernible as a shoulder on the leading edge of bilobide C (BB) (GJ is not labeled in Figure 2F), and its [M − H] was detected at m/z 423 (Figure 4). The retention times were tR 27.02 min for GJ, 27.41 min for BB, 28.04 min for GC, 44.32 min for GA, and 44.84 min for GB, respectively, and perfectly matched those of the standards (not shown).

Comparison of Leaves and Processed Products Using the Phenolic Component Profile

The samples consisted of six G. biloba leaf samples and three processed products from local stores. The four leaf samples that were personally collected (samples 3–6) came from different locations with significantly different altitudes, weather, and soil conditions. Two of the Chinese samples (samples 5 and 6) were from male and female trees at the same location. The female tree produces fruit yearly, and the male tree does not. Both of them grow widely in China. Over 70% of ginkgo leaves and their products come from China, and many studies on the quality of the leaves and products, including profiling methods, have been carried out there (2, 29, 3638). Despite the difference in growing conditions and sex, all 6 leaf samples had similar chromatographic profiles; only the relative intensities of the peaks were different (Figure 5). Thus, the data reported in this study suggest that the G. biloba chromatographic profiles might be similar for ginkgo trees growing all over the world. This is not surprising since G. biloba does not have diverse phenotypes.

Figure 5
LC Chromatograms of Ginkgo biloba leaf samples: (A) sample-1, NIST SRM 3246; (B) sample-2, AHP standard; (C) sample-3, the leaves from the tree in MD, USA; (D) sample-4, the leaves from the tree in Kunming, China; (E) sample-5, the leaves from the female ...

The three processed products contained most of the glycosylated flavonoids found in the raw materials (Figure 6), even acylated flavonoid glycosides (37–50 min). The only substantial difference between the raw leaf and processed materials was the lack of significant amounts of the biflavones in the processed materials.


We thank Professor Da-Yuan Zhu (Shanghai Institute of Materai Medica, Chinese Academy of Sciences) for a gift of ginkgolide J standard, Professor Hundong Sun (Kunmin institute of Botany, Chinese Academy of Sciences) for the G. biloba leaves (sample 4) picked from the tree at his institute, and De-Chang Zhang for the leaves (samples 5 and 6) from the trees in his yard in Wanzhou, Chongqing City, China.

This research was supported by the Agriculture Research Service of the U.S. Department of Agriculture and the Office of Dietary Supplements at the National Institutes of Health.

Literature Cited

1. van Beek TA. Chemical analysis of Ginkgo biloba leaves and extracts. J Chromatogr A. 2002;967:21–55. [PubMed]
2. Editorial Committee of Encyclopedia of Chinese Medicinal Plants. Encyclopedia of Chinese Medicinal Plants. Vol. 2. Shanghai press of Science and Technology; Shanghai, China: 1999. Ginkgo biloba L. Leaves; pp. 280–284.
3. AHPA. Code of Ethics & Business Conduct. American Herbal Products Association; 2006.
4. Mahadevan S, Park Y. Multifaceted therapeutic benefits of Ginkgo biloba L.: chemistry, efficacy, safety, and uses. J Food Sci. 2008;73:R14–19. [PubMed]
5. Harborne JB, Baxter H, editors. The Handbook of Natural Flavonoids. Vol. 1. John Wiley & Sons, Ltd.; New York: 1999. Ginkgo biloba; p. 850. Vol. 1 and Vol. 2, p 839.
6. Hasler A, Sticher O. Identification and determination of flavonoids from Ginkgo biloba by high performance liquid chromatography. J Chromatogr. 1992;605:41–48.
7. Tang Y, Lou F, Wang J, Li Y, Zhuang S. Coumaroyl flavonol glycosides from the leaves of Ginkgo biloba. Phytochemistry. 2001;58:1251–1256. [PubMed]
8. Hasler A, Gross GA, Meier B, Sticher O. Complex flavonol glycosides from the leaves of Ginkgo biloba. Phytochemistry. 1992;31:1391–1394. [PubMed]
9. Hyun SK, Kang SS, Son KH, Chung HY, Choi JS. Biflavone glucosides from Ginkgo biloba yellow leaves. Chem Pharm Bull (Tokyo) 2005;53:1200–1201. [PubMed]
10. Zhang Q, Chen LJ, Ye HY, Gao L, Hou W, Tang M, Yang G, Zhong Z, Yuan Y, Peng A. Isolation and purification of ginkgo flavonol glycosides from Ginkgo biloba leaves by highspeed counter-current chromatography. J Sep Sci. 2007;30:2153–2159. [PubMed]
11. Bedir E, Tatli II, Khan RA, Zhao J, Takamatsu S, Walker LA, Goldman P, Khan IA. Biologically active secondary metabolites from Ginkgo biloba. J Agric Food Chem. 2002;50:3150–3155. [PubMed]
12. Ozcan M, McAuley B, Chen P. Comparison of the terpene lactones and flavonols contents in Ginkgo biloba commercial samples and the NIST standard reference material using LC/UV/MS. J Food Drug Anal. 2007;15:55–62.
13. Chen P, Ozcan M, Harnly J. Chromatographic fingerprint analysis for evaluation of Ginkgo biloba products. Anal Bioanal Chem. 2007;389:251–261. [PMC free article] [PubMed]
14. Ding S, Dudley E, Plummer S, Tang J, Newton RP, Brenton AG. Quantitative determination of major active components in Ginkgo biloba dietary supplements by liquid chromatography/mass spectrometry. Rapid Commun Mass Spectrom. 2006;20:2753–2760. [PubMed]
15. Ding S, Dudley E, Chen L, Plummer S, Tang J, Newton RP, Brenton AG. Determination of active components of Ginkgo biloba in human urine by capillary high-performance liquid chromatography/mass spectrometry with on-line column-switching purification. Rapid Commun Mass Spectrom. 2006;20:3619–3624. [PubMed]
16. Dubber MJ, Kanfer I. High performance liquid chromatographic determination of selected flavonols in Ginkgo biloba solid oral dosage forms. J Pharm Sci. 2004;7:3030–3039. [PubMed]
17. Dubber MJ, Sewram V, Mshicileli N, Shephard GS, Kanfer I. The simultaneous determination of selected flavonol glycosides and aglycones in Ginkgo biloba oral dosage forms by high performance liquid chromatography-electrospray ionizationmass spectrometry. J Pharm Biomed Anal. 2005;37:723–731. [PubMed]
18. Dubber MJ, Kanfer I. Application of reverse-flow micellar electrokinetic chromatography for the simultaneous determination of flavonols and terpene trilactones in Ginkgo biloba dosage forms. J Chromatogr A. 2006;1122:266–274. [PubMed]
19. Ji YB, Xu QS, Hu YZ, Heyden Y. Development, optimization and validation of a fingerprint of Ginkgo biloba extracts by high-performance liquid chromatography. J Chromatogr A. 2005;1066:97–104. [PubMed]
20. Mesbah MK, Khalifa SI, El-Gindy A, Tawfik KA. HPLC determination of certain flavonoids and terpene lactones in selected Ginkgo biloba L. Farmaco. 2005;60:583–590. [PubMed]
21. Deng F, Zito SW. Development and validation of a gas chromatographic-mass spectrometric method for simultaneous identification and quantification of marker compounds including bilobalide, ginkgolides and flavonoids in Ginkgo biloba L. extract and pharmaceutical preparations. J Chromatogr A. 2003;986:121–127. [PubMed]
22. Xie P, Chen S, Liang YZ, Wang X, Tian R, Upton R. Chromatographic fingerprint analysisa rational approach for quality assessment of traditional Chinese herbal medicine. J Chromatogr A. 2006;1112:171–180. [PubMed]
23. Gray DE, Upton R, Chandra A, Porter A, Harris RK. Quantitative analysis of flavonol glycosides in Ginkgo biloba: a comparison of two analytical methods. Phytochem Anal. 2006;17:56–62. [PubMed]
24. Grayrint DE, Messer D, Porter A, Ferguson S, Harris RK, Clark AP, Algaier JW, Overstreet JD, Smith CS. Simultaneous quantification of terpenelactones and flavonol aglycones in hydrolyzed Ginkgo biloba extract by liquid chromatography with inline ultraviolet and evaporative light scattering detection. J-AOAC-Int. 2005;88:1613–1620. erratum in J AOAC Int 2006, 89, 41A. [PubMed]
25. Gray DE, Messer D, Porter A, Hefner B, Logan D, Harris RK, Clark AP, Algier JA, Overstreet JD, Smith CS. Analysis of flavonol aglycones and terpenelactones in Ginkgo biloba extract: A comparison of high-performance thin-layer chromatography and column high-performance liquid chromatography. J AOAC Int. 2007;90:1203–1209. [PubMed]
26. Repollés C, Herrero-MaríInez JM, Ràfols C. Analysis of prominent flavonoid aglycones by high-performance liquid chromatography using a monolithic type column. J Chromatogr A. 2006;1131:51–57. [PubMed]
27. Li W, Fitzloff JF. Simultaneous determination of terpene lactones and flavonoid aglycones in Ginkgo biloba by high-performance liquid chromatography with evaporative light scattering detection. J Pharm Biomed Anal. 2002;30:67–75. [PubMed]
28. Jensen AG, Ndjoko K, Wolfender JL, Hostettmann K, Camponovo F, Soldati F. Liquid chromatography-atmospheric pressure chemical ionization/mass spectrometry: a rapid and selective method for the quantitative determination of terpene lactones and bilobalide in Ginkgo biloba leaf and phytopharmaceuticals. Phytochem Anal. 2002;13:31–38. [PubMed]
29. Li CY, Lin CH, Wu CC, Lee KH, Wu TS. Efficient 1H nuclear magnetic resonance method for improved quality control analyses of Ginkgo constituents. J Agric Food Chem. 2004;52:3721–3725. [PubMed]
30. Elnain-Wojtaszek M, Kruczynski Z, Kasprzak J. Variation in the free radical scavenging activity of G Ginkgo biloba L. leaves in the period of complete development green leaves to fall of yellow ones. Food Chem. 2002;79:79–84.
31. Lin LZ, Qiu SX, Lindenmaier M, He XG, Featherstone T, Cordell GA. Patunetin 3-O-rutinoside from the aerial parts of Echinacea angustifolia. Pharmat Biol. 2002;40:92–95.
32. Lin LZ, Harnly JM. A screening method for the identification of glycosylated flavonoids and other phenolic compounds using a standard analytical approach for all plant materials. J Agric Food Chem. 2007;55:1084–1096. [PMC free article] [PubMed]
33. Mabry TJ, Markham KR, Thomas MB, editors. The Systematic Identification of Flavonoids. Springer-Verlag; New York, NY: 1970. The Ultraviolet Spectra of Flavones and Flavonols; pp. 41–45.
34. Cuyckens F, Cleays M. Mass spectrometry in the structural analysis of flavonoids. J Mass Spectrom. 2004;39:1–15. [PubMed]
35. Son JK, Son MJ, Lee E, Moon TC, Son KH, Kim CH, Kim HP, Kang SS, Chan HW. Ginkgetin, a biflavone from Ginkgo biloba leaves, inhibits cyclooxygenases-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells. Biol Pharm Bull. 2005;28:2181–2184. [PubMed]
36. Wang H, Zhao GB, Liu SQ, Zheng JH. Determination of flavonoids in Ginkgo biloba L. leaves from different producing areas. Zhongguo Zhong Yao Za Zhi. 2000;25:408–410. [PubMed]
37. Xing SY, Wu DJ, Xing LF, You XL, Zhang YP, Sun X, Liu YQ. Quantitative genetic analysis and multiple trait selection of pharmaceutical composition on Ginkgo biloba L. leaves. Yi Chuan Xue Bao. 2002;29:928–935. [PubMed]
38. Fan Y, Wang Y, Tan R, Zhang Z. Seasonal and sexual variety of Ginkgo flavonol glycosides in the leaves of Ginkgo biloba L. Zhongguo Zhong Yao Za Zhi. 1998;23:267–269. [PubMed]