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G3 (Bethesda). Aug 2013; 3(8): 1409–1418.
Published online Aug 1, 2013. doi:  10.1534/g3.113.007112
PMCID: PMC3737180
Mutational Analysis of Sse1 (Hsp110) Suggests an Integral Role for this Chaperone in Yeast Prion Propagation In Vivo
Ciara Moran,* Gemma K. Kinsella, Zai-Rong Zhang,1 Sarah Perrett, and Gary W. Jones*2
*Yeast Genetics Laboratory, Department of Biology, National University of Ireland Maynooth, Maynooth, County Kildare, Ireland
The Marie Curie Laboratory for Membrane Proteins, Department of Biology, National University of Ireland Maynooth, Maynooth, County Kildare, Ireland
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China
Supporting information is available online at http://www.g3journal.org/lookup/suppl/doi:10.1534/g3.113.007112/-/DC1.
1Present address: Department of Cell Biology, Nanobiology Institute, Yale School of Medicine, 850 West Campus Drive, Orange, CT 06516.
2Corresponding author: Department of Biology, National University of Ireland Maynooth, Maynooth, County Kildare, Ireland. E-mail: gary.jones/at/nuim.ie
Received January 19, 2013; Accepted June 12, 2013.
The yeast Hsp110 chaperone Sse1 is a conserved protein that is a noncanonical member of the Hsp70 protein superfamily. Sse1 influences the cellular response to heat stress and has also been implicated in playing a role in the propagation of prions in yeast. Sse1 can seemingly exert its effects in vivo through direct or indirect actions by influencing the nucleotide exchange activity of canonical cytosolic Hsp70s. Using a genetic screen based on the inability to propagate the yeast [PSI+] prion, we have identified 13 new Sse1 mutants that are predicted to alter chaperone function through a variety of different mechanisms. Not only are these new Sse1 mutants altered in the ability to propagate and cure yeast prions but also to varying degrees in the ability to grow at elevated temperatures. The expression levels of chaperone proteins known to influence yeast prion propagation are unaltered in the Sse1 mutants, suggesting that the observed phenotypic effects are caused by direct functional alterations in these mutants. Mapping the location of the mutants onto the Sse1 crystal structure suggests that more than one functional alteration in Sse1 may result in changes in prion propagation and ability to function at elevated temperatures. All Sse1 mutants isolated provide essential functions in the cell under normal growth conditions, further demonstrating that essential chaperone functions in vivo can to some degree at least be detached from those related to propagation of prions. Our results suggest that Sse1 can influence prion propagation through a variety of different mechanisms.
Keywords: Saccharomyces cerevisiae, prion, chaperone, Sse1, Hsp110, Hsp70, nucleotide exchange factor
Hsp110 proteins are a group of eukaryotic molecular chaperones that have been implicated in a variety of cellular functions. Several cytosolic Hsp110 protein variants have been described in eukaryotes, including HSPH1, Apg-1, Apg-2, and Grp170 in mammals (Vos et al. 2008; Kampinga et al. 2009). Hsp110 is represented in Saccharomyces cerevisiae by the Sse1 and Sse2 proteins. SSE1 and SSE2 constitute an essential gene pair in yeast (Trott et al. 2005) and although not essential in itself deletion of SSE1 does confer a growth defect and stress-related phenotypes (Shirayama et al. 1993; Shaner et al. 2004, 2008). Sse1 was first isolated from yeast biochemically as a calmodulin-binding protein (Mukai et al. 1993) and genetically as a suppressor of a protein kinase A (PKA) mutant (Shirayama et al. 1993). Sse1 and Sse2 share a high degree of sequence identity (~76%) and are noncanonical members of the Hsp70 superfamily (Mukai et al. 1993). SSE1 is expressed at moderately high levels under normal growth conditions and is further induced upon heat shock whereas SSE2 transcripts are nearly undetectable at basal temperatures but are increased more than 20-fold upon heat shock (Mukai et al. 1993; Shirayama et al. 1993).
The Sse1 protein has been crystallized and established to be modular, built-up from Hsp70-like subdomains (Liu and Hendrickson 2007). Although Sse1 and canonical Hsp70 have diverged in function, certain structural features in Hsp70 have been conserved in Sse1. Mutational analysis revealed that particular mutant variants of Sse1 and Ssa1 (one of the major yeast cytosolic Hsp70s) result in similar phenotypic defects, supporting the hypothesis that Sse1 is an evolutionary vestige of Hsp70 (Liu and Hendrickson 2007). It has been reported that Sse1, like Ssa1, can recognize and bind hydrophobic peptide sequences with high affinity (Goeckeler et al. 2008) and can exhibit ATPase activity (Raviol et al. 2006a,b). However, the functional similarities end there, as Sse1 cannot functionally refold denatured proteins but instead acts as a “holdase” by binding denatured proteins and preventing their aggregation (Oh et al. 1999). This “holdase” function may serve a function in the peptide-refolding pathway carried out by other chaperones. Various Hsp110 homologs have been shown to accelerate the refolding of luciferase by Hsp70/Hsp40 machinery (Goeckeler et al. 2002).
Although specific intracellular functions of Hsp110 proteins are poorly characterized in comparison with various canonical Hsp70s, it has been suggested recently that they may act as the principal nucleotide exchange factor (NEF) for Hsp70. Sse1 was shown to act as a potent NEF for yeast cytosolic Hsp70 proteins Ssa1 and Ssa2 (Dragovic et al. 2006; Raviol et al. 2006b). This discovery followed soon after the discovery that Hsp110 proteins physically and functionally interact with their Hsp70-Ssa counterparts (Yamagishi et al. 2004; Shaner et al. 2005; Yam et al. 2005). Prior to these findings Fes1 was the only identified NEF for Ssa1 (Kabani et al. 2002a). The regulation of substrate binding by ATP hydrolysis and subsequent nucleotide exchange is a key component in maintaining correct in vivo function for all Hsp70 chaperones.
The general domain organization of Sse1 does reflect that of canonical Hsp70s. It consists of a N-terminal nucleotide-binding domain (NBD), a β-sandwich domain (SBD-β) and a three helical bundle domain (3HBD or SBD-α) toward the C-terminus. The Sse1 protein has a compact structure with tight interactions between the NBD and substrate-binding domain (SBD). Unlike Hsp70, the Sse1 SBD-α does not form a lid over its binding pocket but instead interacts with the flank of the Sse1 NBD (Polier et al. 2008). Sse1 is larger than Hsp70 as the result of insertions within the SBD and a C-terminal extension (Easton et al. 2000; Liu and Hendrickson 2007). Sse1 shares ~30% sequence identity with Ssa1 (Shaner et al. 2005; Yam et al. 2005). Like other Hsp70-Hsp110 interacting components, the sequence similarity between Sse1 and Ssa1 is largely confined to the NBD (Goeckeler et al. 2008).
Sse1 preferentially associates with Ssa1 in vivo (Shaner et al. 2005). The Hsp70 NBD is embraced by the NBD and SBD-α of Sse1, leading to the opening of the Hsp70 nucleotide-binding cleft. The Sse1 β-sandwich domain of the substrate binding cleft alternates away from the complex (Polier et al. 2010). It appears that almost the entire length of Sse1 is required for complex formation with Hsp70 (Shaner et al. 2004; Dragovic et al. 2006; Polier et al. 2008). Complex formation also requires Sse1 to be ATP-bound as this alters the NBD structure in a way that stabilizes it and allows it to bind Hsp70 (Shaner et al. 2006; Polier et al. 2008). Yeast Sse1 can also form a functional complex with human Hsp70, which reflects a high degree of conservation in the Hsp70-Hsp110 structure (Shaner et al. 2006).
The multidomain architecture of Sse1 suggests that it may play a role as a chaperone similar to Hsp70. However, the protein folding ability of canonical Hsp70s relies heavily on the conformational structural changes between the NBD and SBD upon ATP/ADP binding; such allostery appears absent in Sse1. The Sse1 substrate-binding pocket remains closed upon ATP binding, suggesting that any potential substrate-binding or chaperone activity inherent in Sse1 will be functionally distinct to Hsp70 (Andréasson et al. 2008).
Since the seminal paper by Wickner (1994), who proposed that the yeast non-Mendelian genetic elements [PSI+] and [URE3] are prions of the Sup35 and Ure2 proteins, respectively, the authors of many subsequent studies have shown this proposal to be correct and that a significant number of other fungal proteins have prion forming ability (Derkatch et al. 2001; Alberti et al. 2009). A variety of in vitro and in vivo studies have demonstrated an integral role for molecular chaperones in yeast prion propagation (reviewed in, Jones and Tuite 2005; True 2006; Perrett and Jones 2008; Masison et al. 2009). Most chaperone/prion studies have focused upon the yeast Hsp40/Hsp70/Hsp104 protein disaggregation machinery (Chernoff et al. 1995; Glover et al. 1997; Krzewska and Melki 2006; Shorter and Lindquist 2008), which has been shown to play an essential role in propagation of yeast prions. More recently, evidence has accumulated suggesting a role for yeast Hsp110 in prion formation and propagation. Studies have demonstrated Sse1 may be required for the de novo formation and propagation of [PSI+] (Fan et al. 2007; Kryndushkin and Wickner 2007; Sadlish et al. 2008). Current understanding suggests that Sse1 primarily influences prion formation and propagation due to its NEF function for Hsp70; however, Sse1 has been suggested to bind to early intermediates in Sup35 prion conversion and thus facilitate prion seed conversion independently of its NEF function (Sadlish et al. 2008). Overexpressed Sse1 was shown to increase the rate of de novo [PSI+] formation while deleting SSE1 reduced [PSI+] prion formation; however, no effects on pre-existing [PSI+] were observed (Fan et al. 2007; Kryndushkin and Wickner 2007). In contrast, the overproduction or deletion of SSE1 cured the [URE3] prion and mutant analysis suggests this activity is dependent on ATP binding and interaction with Hsp70 (Kryndushkin and Wickner 2007). Intriguingly, Sse1 has recently been shown to function as part of a protein disaggregation system that appears to be conserved in mammalian cells (Shorter 2011; Duennwald et al. 2012).
To gain further insight into the possible functional roles of Hsp110 in prion propagation, we have isolated an array of novel Sse1 mutations that differentially impair the ability to propagate [PSI+]. The locations of these mutants on the Sse1 protein structure suggest that impairment of prion propagation by Hsp110 can occur through a number of independent and distinct mechanisms. The data suggests that Sse1 can influence prion propagation not only indirectly through an Hsp70-dependent NEF activity, but also through a direct mechanism that may involve direct interaction between Sse1 and prion substrates.
Strains and plasmids
Strains and plasmids used and constructed in this study are listed and described in Table 1 and Table 2. Site-directed mutagenesis using the Quickchange kit (Stratagene) and appropriate primers were used to introduce desired mutations into plasmids. The G600 strain, the genome of which was recently sequenced (Fitzpatrick et al. 2011), was used to amplify SSE genes via polymerase chain reaction for cloning into pRS315. The human HSPH1 gene (alternative name HSP105) was amplified from a cDNA clone purchased from Origene (Rockville, MD). All plasmids constructed in this study were verified by sequencing.
Table 1
Table 1
Strains used in this study
Table 2
Table 2
Plasmids used in this study
Media and genetic methods
Standard media was used throughout this study as previously described (Guthrie and Fink 1991). Monitoring of [PSI+] was carried out as described (Jones and Masison 2003). Briefly, the presence of [PSI+] (the non-functional aggregated form of Sup35) and SUQ5 causes efficient translation read through of the ochre mutation in the ade2-1 allele. Non-suppressed ade2-1 mutants are Ade- and are red when grown on medium containing limiting amounts of adenine due to the accumulation of a pigmented substrate of Ade2. Partial suppression of ade2-1 by [PSI+] allows growth without adenine and eliminates the pigmentation (Cox 1965).
Monitoring of [URE3] again made use of the red/white selection based on the ADE2 gene. The strain SB34 has ADE2 under control of the DAL5 promoter. In [URE3] cells expression of the DAL5 promoter is high because of the action of Gln3. In [ure-0] cells soluble Ure2 can interact with Gln3 and prevent transcription from the DAL5 promoter. Hence, when [URE3] is present the SB34 strain will grow on medium lacking adenine and is white on medium with limiting adenine. When [ure-0] this strain will not grow on medium lacking adenine and is red on medium with limiting adenine.
Generation of SSE1 mutant library
Plasmid pRS315-SSE1 was subjected to treatment with hydroxylamine for 60 min (Schatz et al. 1988). This treatment resulted in mutation frequencies of around 8% for this plasmid (G. W. Jones, unpublished data).
Isolation of Sse1 mutants that impair [PSI+] prion propagation
Sse1 mutants were isolated using the plasmid shuffle technique. Strain CMY02 was transformed with the SSE1 mutagenized plasmid library. Transformed cells were selected on medium lacking leucine. Any red or dark-pink colonies were scored at this point as potential dominant SSE1 mutants that could weaken [PSI+]. Transformation plates were replica plated onto medium-containing limiting amounts of adenine and also 5-fluoro-orotic acid, a chemical that selects against URA+ cells and hence against the presence of the pRS316-SSE1 plasmid. Colonies appearing red or dark-pink at this stage were scored as potentially harboring a mutant sse1 allele that cannot maintain [PSI+]. All potential sse1 mutant containing plasmids were isolated and retransformed back into CMY02 and analyzed for their effects upon [PSI+]. After retransformation, the color phenotype of colonies was scored subjectively from 0 to 9, with 0 being white and 9 being red (Loovers et al. 2007).
Assaying mutant effects on [URE3]
Effects on [URE3] were assayed as previously described (Loovers et al. 2007). To summarize, SB34 was grown to log phase growth under conditions that maintain [URE3] (medium lacking adenine). Cells were transformed with wild-type (WT) or mutant SSE1 alleles and transformants were selected on medium lacking leucine. At this stage all cells (at least 100) were scored for color phenotype on the basis of being white, red or sectored.
Mapping mutants onto crystal structure of Sse1 and molecular modeling
Structures for Sse1 (2QXL; (Liu and Hendrickson 2007) and for Sse1 in complex with Ssa1 (3D2F; (Polier et al. 2008) were obtained from the Protein Data Bank. Molecular modeling to complete gap regions, introduce point mutations (100 models each), and for visualization was carried out using Molecular Operating Environment, version 2009.10 (Chemical Computing Group Inc., 2009). Images were generated using pyMol (DeLano 2002).
Western analysis
Western analysis was performed essentially as described previously (Jones and Masison 2003). Hsp70 monoclonal antibody was purchased from Cambridge Bioscience (SPA822), Sse1 polyclonal antibody was a gift from Jeff Brodsky (University of Pittsburgh), and Hsp104 polyclonal antibody was a gift from John Glover (University of Toronto).
Isolation of novel mutants of SSE1 that impair [PSI+] prion propagation
Applying the plasmid shuffle technique as described in Materials and Methods we have identified 13 new mutants of Sse1 that impair propagation of the [PSI+] prion (Figure 1, Table 3). Nine of these mutants are located in the NBD and like previous studies highlight the general functional importance of correct ATPase regulation of Hsp70 chaperones in yeast prion propagation (Jones and Masison 2003; Loovers et al. 2007). The mutants had a wide range of effects on propagation of [PSI+], with some being unable to propagate the prion at all (G41D, G50D, D236N, G342D, E370K, and G616D) to others having minor effects on color phenotype (P37L, C211Y; Table 3 and Figure 1B). The presence or absence of [PSI+] in all mutants was confirmed by mating with a [psi] strain followed by sporulation of any [PSI+] diploids to confirm non-Mendelian segregation and subsequent growth on guanidine hydrochloride to cure the prion (data not shown). As expected, all Sse1 mutants that could not propagate [PSI+] could not grow on medium lacking adenine (Figure 1B). However, surprisingly, all other Sse1 mutants, even ones that had an apparently mild affect on [PSI+], also grew very poorly or not at all on medium lacking adenine (Figure 1B). The reason for these growth results is unknown but perhaps suggests Sse1 may be involved in cellular metabolic pathways that can result in complex nutritional phenotypes. Significantly, none of the mutants had a major adverse effect on cell growth at 30°, suggesting that each mutant is capable of carrying out the essential cellular functions of Sse1 (Table 3). However, at 39° there are major differences in the abilities of the mutants to grow (Table 3, Figure 1B). Deletion of SSE1 causes a 39° temperature-sensitive phenotype (Shaner et al. 2008) and therefore it appears that a subset of mutants (G50D, G342D, S440L, G616D) are effectively nonfunctional at this elevated temperature. Other mutants appear to provide either WT levels of activity (P37L, T365I, E554K) or some intermediate or reduced level of Sse1 functionality (G41D, C211Y, D236N, G343D, E370K, E504K).
Figure 1
Figure 1
(A) Sse1 mutants that impair prion propagation are located in various domains of the protein. Numbers above refer to amino acids that define the boundaries of the nucleotide-binding domain (NDB), linker region (L), substrate-binding domain (SBD), Hsp110 (more ...)
Table 3
Table 3
Relative effects of SSE1 mutants on [PSI+] prion propagation and cell growth
Effects of FES1 overexpression on the ability of Sse1 mutants to propagate [PSI+]
Both Fes1 and Sse1 have been shown to be NEFs for cytosolic Hsp70s (Kabani et al. 2002b; Dragovic et al. 2006; Raviol et al. 2006b) We therefore assessed the ability of Fes1 to complement the prion propagation defect of this novel set of Sse1 mutants. To do this we carried out plasmid shuffle analysis for each Sse1 mutant in the presence of over-expressed Fes1 (Figure 2). As a negative control plasmid shuffle analysis was also carried out in the presence of either pRS423 (vector only) or pRS423 harboring the CIA1 gene ±500 bp. CIA1 is a yeast gene that has not been implicated in altering yeast prion propagation. After growth on 5-fluoro-orotic acid media also lacking histidine (to maintain selection for pRS423 based plasmids), cells were placed onto YPD to assess color and –ADE –HIS medium to assess the ability to grow on medium lacking adenine. Although the color phenotype on YPD for Sse1 WT or mutant cells harboring the vector or overexpressing FES1 is consistent with presence of Sse1 alone (compare Figure 1B YPD panel with Figure 2 control and FES1 YPD panels), the ability of some CMY02 Sse1 mutant cells to grow on medium lacking adenine is influenced greatly by the absence of histidine (compare Figure 1B –ADE panel with Figure 2 control and FES1 –ADE panels). Only G616D appears altered in color on YPD by the presence of FES1 overexpression. However, this color change does not correlate with a significant increased ability to grow on –ADE medium (Figure 2). Comparing the effects of vector only to overexpressed FES1, a clear difference in ability to grow on –ADE medium is observed for some mutants; P37L, C211Y, S440L, and E554K grow less well on –ADE in the presence of overexpressed FES1, whereas G343D and T365I grow slightly better in the presence of overexpressed FES1 (Figure 2), suggests that increases in Hsp70 (Ssa) NEF activity are able to influence some phenotypes of this subset of Sse1 mutants. Currently, we have no explanation for the complex but reproducible –ADE phenotype of these novel Sse1 mutants shown in Figures 1B and and22.
Figure 2
Figure 2
Sse1 mutants exhibit a complex growth phenotype when grown on medium lacking adenine. The absence of histidine and the presence of FES1 can affect the ability of Sse1 mutants to grow on medium lacking adenine. Top section is growth in presence of either (more ...)
Sse1 mutants are defective in ability to cure [URE3] prion
A previous study has highlighted the ability of overexpressed Sse1 to impair propagation of the yeast prion [URE3] (Kryndushkin and Wickner 2007). Similarly we found that in the SB34 strain background (Bach et al. 2003) the introduction of an extra copy of SSE1 under control of its native promoter was capable of causing a significant impairment of [URE3] (Table 4). We therefore assessed the ability of the Sse1 mutants to impair [URE3] propagation using this assay. In contrast to WT Sse1 and in contrast to the diverse phenotypic effects observed in [PSI+] prion propagation and temperature sensitivity assays, we found that all thirteen novel Sse1 mutants were unable to significantly impair [URE3] propagation in the SB34 strain (Table 4). This suggests either a common functional change or defect within these mutants with respect to the ability to cure [URE3] or that more than one functional alteration in Sse1 can impair [URE3] curing ability.
Table 4
Table 4
Relative effects of Sse1 mutants on ability to cure [URE3]
Chaperone abundance in Sse1 mutants
It is well documented that certain chaperones play an essential role in prion maintenance and alteration in expression levels can affect [PSI+] propagation (for review see (Jones and Tuite 2005)). We therefore measured Sse1, Hsp104 and the Hsp70 (Ssa) chaperone family expression levels in all the Sse1 mutants. Figure 3 (and data not shown) shows that no major differences in chaperone expression levels exist between any mutants compared to wild-type Sse1. Only the P37L mutant appeared to have slightly increased levels of Hsp104 and Ssa, but taking into account previous findings these are unlikely to be the cause of any prion or temperature-related phenotypes (Jung et al. 2000; Jones and Masison 2003; Loovers et al. 2007). In addition we also measured levels of Hsp70 co-chaperones Ydj1 and Sis1 and found similar amounts of these Hsp40s within the Sse1 mutants analyzed in Figure 3 compared to wild type (data not shown). Therefore, the phenotypic changes in prion propagation and growth at high temperatures observed in these novel Sse1 mutants is most likely not due to indirect changes in chaperone expression levels.
Figure 3
Figure 3
No change in protein levels of chaperones known to alter [PSI+] propagation in Sse1 mutants. Western blot analysis to measure protein levels of Sse1, Hsp70 (Ssa), and Hsp104. After initial blotting with anti-Sse1 antisera, the membrane was stripped and (more ...)
As shown in Figure 1, a number of Sse1 mutants are unable to grow at 39°. One possible explanation for this phenotype is that such Sse1 mutants are unstable at this temperature. We therefore used Western blotting to assess the stability of Sse1 mutants following exposure to 39° for 1 hr and found no difference in stability between any Sse1 mutants compared to wild-type protein (data not shown).
Location of mutants on crystal structure of Sse1: functional implications
The crystal structure of the Sse1 protein alone and in complex with cytosolic Hsp70 has been determined (Liu and Hendrickson 2007; Polier et al. 2008; Schuermann et al. 2008). To gain insight into possible functional consequences of this new set of Sse1 mutations we mapped mutated residues onto available Sse1 structures and used molecular modeling to predict possible localized structural changes and functional implications (Figure 4, Table 5 and Supporting Information, File S1). Of the nine mutants identified within the NBD four are predicted to affect ATP binding (P37L, G342D, G343D, E370K), three to alter interaction with cytosolic Hsp70 (G41D, T365I, E370K), and three remain unclear (G50D, C211Y, D236N) (Table 5, File S1). The four mutants isolated in the SBD domain are predicted to alter either Sse1 interaction with cytosolic Hsp70 (E554K, G616D, see Figure S3), substrate binding (S440L), or protein−protein interactions (E504K) (Table 5 and Supplemental Information).
Figure 4
Figure 4
Mapping of mutations onto Sse1 structure. (A) Structural model of Sse1 (PDB: 2QXL) with the residues of interest highlighted and in ball and stick format. Domains are colored to correspond to Figure 1A. Images were generated using Pymol (DeLano 2002). (more ...)
Table 5
Table 5
Predicted structural effects of mutants
Sse2 and [PSI+] propagation
Figure S1 shows an alignment of Sse1 and Sse2. Although these proteins share 76% identity, Sse2 is unable to compensate for Sse1 in terms of [PSI+] prion propagation or growth at higher temperatures (Figure 5; Sadlish et al. 2008; Shaner et al. 2008). All but one of our novel Sse1 mutated residues is conserved in Sse2, the nonconserved residue corresponding to position E504 in Sse1, which is Q504 in Sse2. We reasoned that the inability of Sse2 to propagate [PSI+] could be influenced by this residue difference. Using site-directed mutagenesis, we created a Q504E mutant version of Sse2 and assessed the ability of this protein to propagate [PSI+]. In contrast to wild-type Sse2, Sse2Q504E is able to propagate [PSI+], although not to the same degree as Sse1 (Figure 5). Interestingly, although [PSI+] propagation is restored to some degree in Sse2Q504E, the ability to grow at 39° is not (Figure 5). In addition to rendering Sse1 unable to propagate [PSI+], the G616D mutation was one of two Sse1 mutants that also caused a 37° temperature-sensitive phenotype (Figure 5 and data not shown). Similarly, when G616D is introduced into Sse2 the same phenotype was observed, indicating conservation of functional importance of this residue in these two proteins. Combining Q504E and G616D in the Sse2 protein produces similar phenotypes as observed for Sse1 (Figure 5) and further demonstrates the functional conservation between these residues within yeast Sse proteins.
Figure 5
Figure 5
Phenotypic analysis of yeast cells expressing Sse2 as the sole source of Hsp110. Growth of Sse1, Sse2, and Sse2 derived mutants on medium lacking adenine (top growth panels) and at elevated temperature (lower growth panels). Western blotting was used (more ...)
Functional complementation of an sse1 sse2 double deletion strain by FES1 and human HSPH1 is dependent on strain background
A previous study has reported that the essential and prion-related functions of Sse1 were mainly related to the ability of the protein to function as a NEF for Hsp70. This was demonstrated by the ability of Fes1 and a N-terminally truncated Snl1 protein to complement the lethality of an sse1sse2 double deletion strain (Sadlish et al. 2008). We therefore assessed whether Fes1 and the closest human Sse1 ortholog HSPH1 (Figure S2) could propagate [PSI+] in the G600 background. We found that both Fes1 and HSPH1 were unable to complement essential Sse1/2 functions in the CMY02 strain (Figure 6), and hence we were unable to assess whether [PSI+] could be propagated. The inability of Fes1 and HSPH1 to functionally substitute for deletion of sse1 and sse2 is strain specific as both were able to provide essential Sse1/2 functions in strain CMY03, which was constructed in the BY4741 background (Figure 6, Table 1). The cause of this difference in strain complementation is as yet unknown.
Figure 6
Figure 6
Complementation of sse1 sse2 deletion strain by overexpression of FES1 or mammalian HSPH1. Growth of sse1 sse2 expressing FES1 or HSPH1 in place of SSE1 was assessed in two strain backgrounds; CMY02 (G600 background, left section) and CMY03 (BY background, (more ...)
We have identified 13 novel mutations in Sse1 that have varying effects on both the ability of S. cerevisiae to propagate the [PSI+] prion and also to grow at increased temperatures. In contrast, all Sse1 mutants were similarly impaired in the ability to cure the [URE3] prion following overexpression. The phenotypic effects of these mutants appear to result from functional changes in the Sse1 protein and are not due to changes in expression levels of other chaperones known to influence prion propagation. Given the varied locations of these mutants in the Sse1 molecule and their predicted structural effects, we provide evidence to suggest that Sse1 can influence both yeast prion propagation and heat shock response in a variety of ways, which are potentially direct or indirect in manner.
Recently, Sse1 has been shown to play a role in the disaggregation of amyloid aggregates, including Sup35 (Shorter 2011; Rampelt et al. 2012). In combination with Hsp40 and Hsp70, Sse1 can dissolve amyloid aggregates albeit at a slower rate than Hsp104. Sse1 also can enhance disaggregation by Hsp104 (in the presence of Hsp40 and 70). This new role for Hsp110 proteins is conserved across species and provides the first clearly identified protein disaggregation machinery in mammalian cells (Shorter 2011; Duennwald et al. 2012). This newly discovered biochemical activity of Sse1 and the fact that Sse1 appears to interact directly with Sup35 prions in vivo (Bagriantsev et al. 2008) suggests that this chaperone may play a more direct and active role in modulating the propagation of yeast prions than was previously thought. Sse1 may influence prion propagation through influencing Ssa1 function but may also do so through interacting directly with prion aggregates. The diverse range of Sse1 mutants we have isolated in this genetic screen and their potential functional implications (Table 5 and Supplemental Information), supports this proposal.
Phenotypic analysis of the Sse1 mutants revealed subsets of mutants that were impaired to varying degrees in their ability to grow at elevated temperatures (Figure 1, Table 3). These results were very clear-cut and presumably are a consequence of altered Sse1 function due to the structural alterations. However, [PSI+] and corresponding adenine growth phenotypes of the mutants was very complex (Figure 1 and Figure 2, Table 3). The colony color phenotype initially used for screening and assessing the presence of [PSI+] was very clear; that is to say, the presence or absence of [PSI+] correlated well with the colony color phenotype. In contrast, the ability to grow on medium lacking adenine did not correlate well for all the mutants. As expected those mutants shown not to propagate [PSI+] did not grow on –ADE medium. However, some Sse1 mutants confirmed as maintaining [PSI+] were also unable to grow on medium lacking adenine. Furthermore, the removal of histidine from the medium can influence the ability of some Sse1 mutants to grow in the absence of adenine and the subsequent overexpression of FES1 can further affect this phenotype (Figure 2). Currently, we do not have any explanation for this very complex but reproducible phenotype, but speculate that Sse1 may play a role (direct or indirect) in modulating the histidine and/or adenine biosynthetic pathways. Both pathways are part of the “super-pathway of histidine, purine and pyrimidine biosynthesis” (Saccharomyces Genome Database) and converge on production of the biosynthetic intermediate aminoimidazole carboxamide ribonucleotide, accumulation of which can be toxic to the cell. If Sse1 is involved in modulating this super-pathway then our mutants may be affected in the ability to synthesize either histidine or adenine (or both) and toxic intermediates on this pathway may also be caused to accumulate. The addition of histidine or adenine to growth medium would have the effect of switching off these pathways and therefore suppressing any impaired growth phenotype due to the accumulation of toxic intermediates.
Given the variation in the effects of mutants upon [PSI+] propagation and also heat shock we were surprised to discover that all the Sse1 mutants were unable to efficiently cure the [URE3] prion. In a previous study, Kryndushkin and Wickner (2007) demonstrated that overexpression of the Sse1G223D mutant (reduction in Sse1 ATPase, interaction with Ssa1 and loss of Ssa1 NEF activity) was unable to cure [URE3] whereas Sse1K69M (can bind ATP but defective in hydrolysis) efficiently cured [URE3]. Thus, it seemed that efficient Sse1 NEF activity is required to cure [URE3]. Our data suggest that this may be an oversimplification. The clear phenotypic differences observed for the Sse1 mutants in respect of [PSI+] propagation and heat shock cannot be explained by a single unifying change in Sse1 function in all mutants. This suggestion is also supported by the location of the mutations on the Sse1 structure. Therefore it appears that a variety of mechanisms that alter Sse1 function can alter the ability to cure [URE3]. However, it should be noted that the ability to cure [URE3] could be influenced by the prion variant that is present in the cells. The [URE3] variants present in the SB34 strain and strains used by Kryndushkin and Wickner (2007) have not been compared directly.
Although Sse1 and Sse2 share a high degree of amino acid sequence identity (Figure S1), Sse2 is unable to compensate fully for the loss of Sse1. Sse2 has previously been shown to compensate for all sse1-deficient phenotypes at 30° (Shaner et al. 2004); however, this is not the case for [PSI+] propagation (Figure 5). In the G600 strain background, the loss of Sse1 function causes loss of [PSI+], demonstrating a clear distinction in the activities of Sse1 and Sse2 at 30°. The fact that the Sse1 mutants that have the greatest impairment of [PSI+] propagation are predicted to be altered in ATP binding and interaction with Hsp70 suggests that in vivo these activities are where Sse1 and Sse2 will differ the most. However, of all 13 mutated residues isolated in Sse1 identified as altering prion propagation, only one (E504) is not conserved in Sse2 (Q504) (Figure S1). We reasoned that this residue contributes to the inability of Sse2 to propagate [PSI+]. When this residue is mutated to create Sse2Q504E [PSI+] can be propagated albeit not to the same extent as Sse1 (Figure 5). This result suggests that this residue is a key factor in dictating divergence of Sse1 and Sse2 function, and this residue is not predicted to alter ATP-binding or interaction with Hsp70. Hence, it appears that the in vivo differences in function between Sse1 and Sse2 are probably attributable to a number of different modifications in activity and not solely to one distinct difference. Clearly the interaction with Hsp70 is a key factor for in vivo function of Sse1 and Sse2 as demonstrated by the conserved effects of the G616D mutation (Figure 5). The combining of the Q504E and G616D mutation in the Sse2 protein produces similar phenotypic responses as for the same Sse1 variant. This indicates the functional conservation of these residues in yeast Sse proteins.
The conservation of essential in vivo functions carried out by Sse1 is clearly shown by the ability of the closest human homolog HSPH1 to complement the growth phenotype of a sse1sse2 deletion strain. A recently characterized Hsp110 ortholog from Arabidopsis thaliana (AtHsp70-15) was shown to be unable to complement heat shock phenotypes of a sse1 deletion strain constructed in the W303 background (Jungkunz et al. 2011). The G600 background used in this study is currently the most closely related sequenced laboratory strain to the original reference yeast strain S288C (Fitzpatrick et al. 2011) and yet there is a background-specific effect on the ability of HSPH1 to complement Sse defects. Hence, testing the AtHsp70-15 cDNA for complementation of sse deletion strains in different yeast backgrounds is certainly worth investigating and may demonstrate further the conservation of Hsp110 essential functions across diverse species.
The isolation of a set of new Sse1 mutants that alter yeast prion propagation has provided further evidence of an integral role for this chaperone in modulating the propagation of [PSI+] and perhaps the growing list of confirmed yeast prions. This set of newly characterized Sse1 mutants provides the opportunity for detailed biochemical assessment to address the causes of subtle differences that may exist in the functional alterations of Sse1 that effect activities in prion propagation as compared to other roles in heat shock or stress resistance. The canonical Hsp70 (Ssa) family is well characterized in its ability to modulate prion propagation and how this function can be distinct from roles in the heat shock response (Jung et al. 2000; Jones and Masison 2003; Loovers et al. 2007). To some degree, the same may be true for Sse1.
Acknowledgments
We thank Jeff Brodsky and John Glover for providing reagents used in this study and also Harriët Loovers for construction of sse1 and sse2 single deletion strains. This work was supported by Science Foundation Ireland Research Frontiers grant (RFP/07/BICF493) awarded to G.W.J. C.M. was a recipient of a postgraduate research scholarship from the Irish Research Council for Science and Engineering Technology. G.K.K. is supported by the Health Research Board. S.P. acknowledges the 973 Program (2012CB911000, 2013CB910700) and the National Natural Science Foundation of China (31070656, 31000342, 31110103914).
Footnotes
Communicating editor: J. Rine
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