Search tips
Search criteria 


Logo of jcinvestThe Journal of Clinical InvestigationCurrent IssueArchiveSubscriptionAbout the Journal
J Clin Invest. 1982 May; 69(5): 1119–1125.
PMCID: PMC370176

Insulin stimulation of adipose tissue lipoprotein lipase. Use of the euglycemic clamp technique.


The role of insulin in the regulation of adipose tissue lipoprotein lipase activity in humans was investigated in 11 normal subjects and compared with the effects of 0.9% saline infusions in five control subjects. After a basal adipose tissue biopsy for lipoprotein lipase activity, insulin was rapidly infused to achieve and maintain serum levels of approximately 70 microunits/ml while plasma glucose was kept at basal concentrations. Free fatty acids in serum fell to 27 +/- 3% of basal by 20 min (t = 5.19, P less than 0.001) and triglycerides decreased to 77 +/- 3% of basal by 80 min (t = 3.76, P less than 0.01). Adipose tissue lipoprotein lipase activity failed to increase significantly above that measured in controls by the first 3 h of the study. By 6 h of the infusion a stimulatory effect of insulin on adipose tissue lipoprotein lipase was found (t = 3.94, P less than 0.01). There was no relationship between the amount of glucose infused and the insulin effect on the enzyme. The increase in adipose tissue lipoprotein lipase activity at 6 h, however, was inversely related to the basal lipase activity (r = -0.690, P less than 0.02). Thus, insulin appears to stimulate adipose tissue lipoprotein lipase activity in humans. This effect of insulin is delayed when compared with antilipolysis and the fall in plasma triglyceride. The inverse relationship between insulin-stimulated adipose tissue lipoprotein lipase activity and basal enzyme activity suggests that adipose tissue itself is the main regulator of the lipase response to insulin.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1021K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Scow RO, Blanchette-Mackie EJ, Smith LC. Transport of lipid across capillary endothelium. Fed Proc. 1980 Jul;39(9):2610–2617. [PubMed]
  • Hollenberg CH. The origin and glyceride distribution of fatty acids in rat adipose tissue. J Clin Invest. 1966 Feb;45(2):205–216. [PMC free article] [PubMed]
  • Taskinen MR, Nikkilä EA. Lipoprotein lipase activity in adipose tissue and in postheparin plasma in human obesity. Acta Med Scand. 1977;202(5):399–408. [PubMed]
  • Ashby P, Robinson DS. Effects of insulin, glucocorticoids and adrenaline on the activity of rat adipose-tissue lipoprotein lipids. Biochem J. 1980 Apr 15;188(1):185–192. [PubMed]
  • Eckel RH, Fujimoto WY, Brunzell JD. Insulin regulation of lipoprotein lipase in cultured 3T3-L1 cells. Biochem Biophys Res Commun. 1978 Oct 30;84(4):1069–1075. [PubMed]
  • Spooner PM, Chernick SS, Garrison MM, Scow RO. Insulin regulation of lipoprotein lipase activity and release in 3T3-L1 adipocytes. Separation and dependence of hormonal effects on hexose metabolism and synthesis of RNA and protein. J Biol Chem. 1979 Oct 25;254(20):10021–10029. [PubMed]
  • Borensztajn J, Samols DR, Rubenstein AH. Effects of insulin on lipoprotein lipase activity in the rat heart and adipose tissue. Am J Physiol. 1972 Dec;223(6):1271–1275. [PubMed]
  • Garfinkel AG, Nilsson-ehle P, Schotz MC. Regulation of lipoprotein lipase. Induction by insulin. Biochim Biophys Acta. 1976 Feb 23;424(2):264–273. [PubMed]
  • Taskinen MR, Nikkilä EA. Lipoprotein lipase activity of adipose tissue and skeletal muscle in insulin-deficient human diabetes. Relation to high-density and very-low-density lipoproteins and response to treatment. Diabetologia. 1979 Dec;17(6):351–356. [PubMed]
  • Nikkilä EA, Huttunen JK, Ehnholm C. Postheparin plasma lipoprotein lipase and hepatic lipase in diabetes mellitus. Relationship to plasma triglyceride metabolism. Diabetes. 1977 Jan;26(1):11–21. [PubMed]
  • Pykälistö OJ, Smith PH, Brunzell JD. Determinants of human adipose tissue lipoprotein lipase. Effect of diabetes and obesity on basal- and diet-induced activity. J Clin Invest. 1975 Nov;56(5):1108–1117. [PMC free article] [PubMed]
  • Taylor KG, Galton DJ, Holdsworth G. Insulin-independent diabetes: a defect in the activity of lipoprotein lipase in adipose tissue. Diabetologia. 1979 May;16(5):313–317. [PubMed]
  • Nilsson-Ehle P, Carlström S, Belfrage P. Rapid effect on lipoprotein lipase activity in adipose tissue of humans after carbohydrate and lipid intake. Scand J Clin Lab Invest. 1975 Jul;35(4):373–378. [PubMed]
  • Goldberg AP, Chait A, Brunzell JD. Postprandial adipose tissue lipoprotein lipase activity in primary hypertriglyceridemia. Metabolism. 1980 Mar;29(3):223–229. [PubMed]
  • Chen YD, Howard J, Huang V, Kraemer FB, Reaven GM. Dissociation between plasma triglyceride concentration and tissue lipoprotein lipase deficiency in insulin-deficient rats. Diabetes. 1980 Aug;29(8):643–647. [PubMed]
  • Insel PA, Liljenquist JE, Tobin JD, Sherwin RS, Watkins P, Andres R, Berman M. Insulin control of glucose metabolism in man: a new kinetic analysis. J Clin Invest. 1975 May;55(5):1057–1066. [PMC free article] [PubMed]
  • Keys A, Fidanza F, Karvonen MJ, Kimura N, Taylor HL. Indices of relative weight and obesity. J Chronic Dis. 1972 Jul 1;25(6):329–343. [PubMed]
  • Richmond W. Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clin Chem. 1973 Dec;19(12):1350–1356. [PubMed]
  • Lopes-Virella MF, Stone P, Ellis S, Colwell JA. Cholesterol determination in high-density lipoproteins separated by three different methods. Clin Chem. 1977 May;23(5):882–884. [PubMed]
  • Desbuquois B, Aurbach GD. Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays. J Clin Endocrinol Metab. 1971 Nov;33(5):732–738. [PubMed]
  • Chlouverakis C, Hojnicki D. A modified radiochemical assay for serum free fatty acid determination. Clin Chim Acta. 1974 Jul 15;54(1):91–93. [PubMed]
  • Passon PG, Peuler JD. A simplified radiometric assay for plasma norepinephrine and epinephrine. Anal Biochem. 1973 Feb;51(2):618–631. [PubMed]
  • Belfrage P, Vaughan M. Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides. J Lipid Res. 1969 May;10(3):341–344. [PubMed]
  • Brunzell JD, Porte D, Jr, Bierman EL. Abnormal lipoprotein-lipase-mediated plasma triglyceride removal in untreated diabetes mellitus associated with hypertriglyceridemia. Metabolism. 1979 Sep;28(9):901–907. [PubMed]
  • Havel RJ, Kane JP, Balasse EO, Segel N, Basso LV. Splanchnic metabolism of free fatty acids and production of triglycerides of very low density lipoproteins in normotriglyceridemic and hypertriglyceridemic humans. J Clin Invest. 1970 Nov;49(11):2017–2035. [PMC free article] [PubMed]
  • Rowe JW, Young JB, Minaker KL, Stevens AL, Pallotta J, Landsberg L. Effect of insulin and glucose infusions on sympathetic nervous system activity in normal man. Diabetes. 1981 Mar;30(3):219–225. [PubMed]

Articles from The Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation