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Mol Cell Biol. 1982 December; 2(12): 1514–1523.
PMCID: PMC369960

Isolation of the CAR1 Gene from Saccharomyces cerevisiae and Analysis of Its Expression


We isolated the CARI gene from Saccharomyces cerevisiae on a recombinant plasmid and localized it to a 1.58-kilobase DNA fragment. The cloned gene was used as a probe to analyze polyadenylated RNA derived from wild-type and mutant cells grown in the presence and absence of an inducer. Wild-type cells grown without the inducer contained very little polyadenylated RNA capable of hybridizing to the isolated CAR1 gene. A 1.25-kilobase CAR1-specific RNA species was markedly increased, however, in wild-type cells grown in the presence of inducer and in constitutive, regulatory mutants grown without it. No CAR1-specific RNA was observed when one class of constitutive mutant was grown in medium containing a good nitrogen source, such as asparagine. Two other mutants previously shown to be resistant to nitrogen repression contained large quantities of CAR1 RNA regardless of the nitrogen source in the medium. These data point to a qualitative correlation between the steady-state levels of CAR1-specific, polyadenylated RNA and the degree of arginase induction and repression observed in the wild type and in strains believed to carry regulatory mutations. Therefore, they remain consistent with our earlier suggestion that arginase production is probably controlled at the level of gene expression.

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Selected References

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  • Bossinger J, Cooper TG. Molecular events associated with induction of arginase in Saccharomyces cerevisiae. J Bacteriol. 1977 Jul;131(1):163–173. [PMC free article] [PubMed]
  • Broach JR, Atkins JF, McGill C, Chow L. Identification and mapping of the transcriptional and translational products of the yeast plasmid, 2mu circle. Cell. 1979 Apr;16(4):827–839. [PubMed]
  • Cameron JR, Loh EY, Davis RW. Evidence for transposition of dispersed repetitive DNA families in yeast. Cell. 1979 Apr;16(4):739–751. [PubMed]
  • Cooper TG, Magasanik B. Transcription of the lac operon of Escherichia coli. J Biol Chem. 1974 Oct 25;249(20):6556–6561. [PubMed]
  • Cooper TG, Whitney P, Magasanik B. Reaction of lac-specific ribonucleic acid from Escherichia coli with lac deoxyribonucleic acid. J Biol Chem. 1974 Oct 25;249(20):6548–6555. [PubMed]
  • Dubois EL, Wiame JM. Catabolic synergism: a cooperation between the availability of substrate and the need for nitrogen in the regulation of arginine catabolism in Saccharomyces cerevisiae. Mol Gen Genet. 1978 Sep 8;164(3):275–283. [PubMed]
  • Errede B, Cardillo TS, Sherman F, Dubois E, Deschamps J, Wiame JM. Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes. Cell. 1980 Nov;22(2 Pt 2):427–436. [PubMed]
  • Fyrberg EA, Kindle KL, Davidson N, Kindle KL. The actin genes of Drosophila: a dispersed multigene family. Cell. 1980 Feb;19(2):365–378. [PubMed]
  • Hinnen A, Hicks JB, Fink GR. Transformation of yeast. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929–1933. [PubMed]
  • Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. [PubMed]
  • Mandel M, Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. [PubMed]
  • Middelhoven WJ. Enzyme repression in the arginine pathway of Saccharomyces cerevisiae. Antonie Van Leeuwenhoek. 1969;35(2):215–226. [PubMed]
  • Nasmyth KA, Reed SI. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2119–2123. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Roeder GS, Fink GR. DNA rearrangements associated with a transposable element in yeast. Cell. 1980 Aug;21(1):239–249. [PubMed]
  • Struhl K, Stinchcomb DT, Scherer S, Davis RW. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035–1039. [PubMed]
  • Whitney PA, Magasanik B. The induction of arginase in Saccharomyces cerevisiae. J Biol Chem. 1973 Sep 10;248(17):6197–6202. [PubMed]
  • Wickerham LJ. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed]
  • Williamson VM, Young ET, Ciriacy M. Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II. Cell. 1981 Feb;23(2):605–614. [PubMed]
  • Yang R, Lis J, Wu R. Elution of DNA from agarose gels after electrophoresis. Methods Enzymol. 1979;68:176–182. [PubMed]

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