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Mol Cell Biol. 1982 November; 2(11): 1427–1435.
PMCID: PMC369947

Genetic analysis of alpha-fetoprotein synthesis in mice.

Abstract

The differential induction of alpha-fetoprotein (AFP) mRNA during liver regeneration in three inbred strains of mice was examined to determine the genetic and molecular bases for the differences in protein production. BALB/cJ, C3H/He, and C57BL/6 mice, previously identified as high, intermediate, and low AFP producers, respectively, were used. Liver AFP mRNA concentrations during normal development and after carbon tetrachloride administration were measured and shown to correlate exactly with the serum protein concentrations. By performing a series of genetic crosses, we identified two unlinked genetic loci that acted independently to affect the inducibility of AFP mRNA. The raf gene, previously identified by Olsson et al. (J. Exp. Med. 145:819-827, 1977), determines the adult basal level of AFP mRNA, and the Rif gene affects its inducibility during regeneration. By using a polymorphic restriction endonuclease site within the albumin-AFP structural gene region, we show that neither regulatory gene is closely linked to the structural genes. In addition, neither gene affects the concentration of albumin mRNA during development or liver regeneration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abelev GI. Alpha-fetoprotein in ontogenesis and its association with malignant tumors. Adv Cancer Res. 1971;14:295–358. [PubMed]
  • Aviv H, Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. [PubMed]
  • Berridge MV, Lane CD. Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins. Cell. 1976 Jun;8(2):283–297. [PubMed]
  • Britten RJ, Graham DE, Neufeld BR. Analysis of repeating DNA sequences by reassociation. Methods Enzymol. 1974;29:363–418. [PubMed]
  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [PubMed]
  • D'Eustachio P, Ingram RS, Tilghman SM, Ruddle FH. Murine alpha-fetoprotein and albumin: two evolutionarily linked proteins encoded on the same mouse chromosome. Somatic Cell Genet. 1981 May;7(3):289–294. [PubMed]
  • Gorin MB, Cooper DL, Eiferman F, van de Rijn P, Tilghman SM. The evolution of alpha-fetoprotein and albumin. I. A comparison of the primary amino acid sequences of mammalian alpha-fetoprotein and albumin. J Biol Chem. 1981 Feb 25;256(4):1954–1959. [PubMed]
  • Gorin MB, Tilghman SM. Structure of the alpha-fetoprotein gene in the mouse. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1351–1355. [PubMed]
  • Honjo T, Packman S, Swan D, Nau M, Leder P. Organization of immunoglobulin genes: reiteration frequency of the mouse kappa chain constant region gene. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3659–3663. [PubMed]
  • Ingram RS, Scott RW, Tilghman SM. alpha-Fetoprotein and albumin genes are in tandem in the mouse genome. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4694–4698. [PubMed]
  • Jalanko H. Alpha-fetoprotein and gamma-glutamyltranspeptidase in mice. Effect of Raf gene. Int J Cancer. 1979 Oct 15;24(4):394–397. [PubMed]
  • Kioussis D, Eiferman F, van de Rijn P, Gorin MB, Ingram RS, Tilghman SM. The evolution of alpha-fetoprotein and albumin. II. The structures of the alpha-fetoprotein and albumin genes in the mouse. J Biol Chem. 1981 Feb 25;256(4):1960–1967. [PubMed]
  • Kuhlmann WD. Immunoperoxidase labelling of alpha 1-fetoprotein (AFP) in normal and regenerating livers of a low and a high AFP producing mouse strain. Histochemistry. 1979 Nov;64(1):67–75. [PubMed]
  • Leys EJ, Kellems RE. Control of dihydrofolate reductase messenger ribonucleic acid production. Mol Cell Biol. 1981 Nov;1(11):961–971. [PMC free article] [PubMed]
  • Maniatis T, Jeffrey A, Kleid DG. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. [PubMed]
  • O'Farrell PH, Kutter E, Nakanishi M. A restriction map of the bacteriophage T4 genome. Mol Gen Genet. 1980;179(2):421–435. [PMC free article] [PubMed]
  • Olsson M, Lindahl G, Ruoslahti E. Genetic control of alpha-fetoprotein synthesis in the mouse. J Exp Med. 1977 Apr 1;145(4):819–827. [PMC free article] [PubMed]
  • Potter M, Wax JS. Genetics of susceptibility to pristane-induced plasmacytomas in BALB/cAn: reduced susceptibility in BALB/cJ with a brief description of pristane-induced arthritis. J Immunol. 1981 Oct;127(4):1591–1595. [PubMed]
  • Ruoslahti E, Seppälä M. alpha-Fetoprotein in cancer and fetal development. Adv Cancer Res. 1979;29:275–346. [PubMed]
  • Schultze B, Gerhard H, Schump E, Maurer W. Autoradiographische Untersuchung über die Proliferation der Hepatocyten be der Regeneration der CCl4-Leber der Maus. Virchows Arch B Cell Pathol. 1973 Dec 17;14(4):329–343. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Taketa K, Watanabe A. Interconvertible microheterogeneity of glucose-6-phosphate dehydrogenase in rat liver. Biochim Biophys Acta. 1971 Apr 14;235(1):19–26. [PubMed]
  • Thomas PS. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. [PubMed]
  • Tilghman SM, Belayew A. Transcriptional control of the murine albumin/alpha-fetoprotein locus during development. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5254–5257. [PubMed]
  • Tilghman SM, Kioussis D, Gorin MB, Ruiz JP, Ingram RS. The presence of intervening sequences in the alpha-fetoprotein gene of the mouse. J Biol Chem. 1979 Aug 10;254(15):7393–7399. [PubMed]
  • Wahl GM, Stern M, Stark GR. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. [PubMed]
  • Wu C, Bingham PM, Livak KJ, Holmgren R, Elgin SC. The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence. Cell. 1979 Apr;16(4):797–806. [PubMed]

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