Search tips
Search criteria 


Logo of molcellbPermissionsJournals.ASM.orgJournalMCB ArticleJournal InfoAuthorsReviewers
Mol Cell Biol. 1982 February; 2(2): 171–178.
PMCID: PMC369770

Developmental regulation of a sporulation-specific enzyme activity in Saccharomyces cerevisiae.


An alpha-glucosidase activity (SAG) occurs in a/alpha Saccharomyces cerevisiae cells beginning at about 8 to 10 h after the initiation of sporulation. This enzyme is responsible for the rapid degradation of intracellular glycogen which follows the completion of meiosis in these cells. SAG differs from similar activities present in vegetative cells and appears to be a sporulation-specific enzyme. Cells arrested at various stages in sporulation (DNA replication, recombination, meiosis I, and meiosis II) were examined for SAG activity; the results show that SAG appearance depends on DNA synthesis and some recombination events but not on the meiotic divisions.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Alton TH, Lodish HF. Developmental changes in messenger RNAs and protein synthesis in Dictyostelium discoideum. Dev Biol. 1977 Oct 1;60(1):180–206. [PubMed]
  • Barnett T, Pachl C, Gergen JP, Wensink PC. The isolation and characterization of Drosophila yolk protein genes. Cell. 1980 Oct;21(3):729–738. [PubMed]
  • Cheung KK, Newton A. Patterns of protein synthesis during development in Caulobacter crescentus. Dev Biol. 1977 Apr;56(2):417–425. [PubMed]
  • Colonna WJ, Gentile JM, Magee PT. Inhibiton by sulfanilamide of sporulation in Saccharomyces cerevisiae. Can J Microbiol. 1977 Jun;23(6):659–671. [PubMed]
  • Colonna WJ, Magee PT. Glycogenolytic enzymes in sporulating yeast. J Bacteriol. 1978 Jun;134(3):844–853. [PMC free article] [PubMed]
  • Davidow LS, Goetsch L, Byers B. Preferential Occurrence of Nonsister Spores in Two-Spored Asci of SACCHAROMYCES CEREVISIAE: Evidence for Regulation of Spore-Wall Formation by the Spindle Pole Body. Genetics. 1980 Mar;94(3):581–595. [PubMed]
  • Esposito MS, Esposito RE. Genes controlling meiosis and spore formation in yeast. Genetics. 1974 Sep;78(1):215–225. [PubMed]
  • Esposito MS, Esposito RE, Arnaud M, Halvorson HO. Acetate utilization and macromolecular synthesis during sporulation of yeast. J Bacteriol. 1969 Oct;100(1):180–186. [PMC free article] [PubMed]
  • Esposito RE, Frink N, Bernstein P, Esposito MS. The genetic control of sporulation in Saccharomyces. II. Dominance and complementation of mutants of meiosis and spore formation. Mol Gen Genet. 1972;114(3):241–248. [PubMed]
  • Fosset M, Muir LW, Nielsen LD, Fischer EH. Purification and properties of yeast glycogen phosphorylase a and b. Biochemistry. 1971 Oct 26;10(22):4105–4113. [PubMed]
  • Fritz H, Hartwich G, Werle E. Uber Proteaseinhibitoren. I. Isolierung und Charakterisierung des Trypsininhibitors aus Pankreasgewebe und Pankreassekret vom Hund. Hoppe Seylers Z Physiol Chem. 1966;345(2):150–167. [PubMed]
  • Game JC, Mortimer RK. A genetic study of x-ray sensitive mutants in yeast. Mutat Res. 1974 Sep;24(3):281–292. [PubMed]
  • Game JC, Zamb TJ, Braun RJ, Resnick M, Roth RM. The Role of Radiation (rad) Genes in Meiotic Recombination in Yeast. Genetics. 1980 Jan;94(1):51–68. [PubMed]
  • Hartwell LH. Saccharomyces cerevisiae cell cycle. Bacteriol Rev. 1974 Jun;38(2):164–198. [PMC free article] [PubMed]
  • Hereford LM, Hartwell LH. Sequential gene function in the initiation of Saccharomyces cerevisiae DNA synthesis. J Mol Biol. 1974 Apr 15;84(3):445–461. [PubMed]
  • HOPKINS RH, KULKA D. The glucamylase and debrancher of S. diastaticus. Arch Biochem Biophys. 1957 Jul;69:45–55. [PubMed]
  • Hopper AK, Magee PT, Welch SK, Friedman M, Hall BD. Macromolecule synthesis and breakdown in relation to sporulation and meiosis in yeast. J Bacteriol. 1974 Aug;119(2):619–628. [PMC free article] [PubMed]
  • Kraig E, Haber JE. Messenger ribonucleic acid and protein metabolism during sporulation of Saccharomyces cerevisiae. J Bacteriol. 1980 Dec;144(3):1098–1112. [PMC free article] [PubMed]
  • Lai HY, Axelrod B. The specificity of the synthetic reaction of two yeast alpha-glucosidases. Biochim Biophys Acta. 1975 May 23;391(1):121–128. [PubMed]
  • Lillie SH, Pringle JR. Reserve carbohydrate metabolism in Saccharomyces cerevisiae: responses to nutrient limitation. J Bacteriol. 1980 Sep;143(3):1384–1394. [PMC free article] [PubMed]
  • Loomis WF., Jr Developmental regulation of alkaline phosphatase in Dictyostelium discoideum. J Bacteriol. 1969 Oct;100(1):417–422. [PMC free article] [PubMed]
  • Loomis WF, White S, Dimond RL. A sequence of dependent stages in the development of Dictyostelium discoideum. Dev Biol. 1976 Oct 15;53(2):171–177. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Malone RE, Esposito RE. The RAD52 gene is required for homothallic interconversion of mating types and spontaneous mitotic recombination in yeast. Proc Natl Acad Sci U S A. 1980 Jan;77(1):503–507. [PubMed]
  • Needleman RB, Federoff HJ, Eccleshall TR, Buchferer B, Marmur J. Purification and characterization of an alpha-glucosidase from Saccharomyces carlsbergensis. Biochemistry. 1978 Oct 31;17(22):4657–4661. [PubMed]
  • Osley MA, Newton A. Temporal control of the cell cycle in Caulobacter crescentus: roles of DNA chain elongation and completion. J Mol Biol. 1980 Mar 25;138(1):109–128. [PubMed]
  • Piñon R, Salts Y, Simchen G. Nuclear and mitochondrial DNA synthesis during yeast sporulation. Exp Cell Res. 1974 Feb;83(2):231–238. [PubMed]
  • Prakash S, Prakash L, Burke W, Montelone BA. Effects of the RAD52 Gene on Recombination in SACCHAROMYCES CEREVISIAE. Genetics. 1980 Jan;94(1):31–50. [PubMed]
  • Pringle JR. Methods for avoiding proteolytic artefacts in studies of enzymes and other proteins from yeasts. Methods Cell Biol. 1975;12:149–184. [PubMed]
  • Resnick MA. Genetic control of radiation sensitivity in Saccharomyces cerevisiae. Genetics. 1969 Jul;62(3):519–531. [PubMed]
  • Roth R, Halvorson HO. Sporulation of yeast harvested during logarithmic growth. J Bacteriol. 1969 May;98(2):831–832. [PMC free article] [PubMed]
  • Roth R, Lusnak K. DNA synthesis during yeast sporulation: genetic control of an early developmental event. Science. 1970 Apr 24;168(3930):493–494. [PubMed]
  • Roth R, Sussman M. Trehalose 6-phosphate synthetase (uridine diphosphate glucose: d-glucose 6-phosphate 1-glucosyltransferase) and its regulation during slime mold development. J Biol Chem. 1968 Oct 10;243(19):5081–5087. [PubMed]
  • Schild D, Byers B. Meiotic effects of DNA-defective cell division cycle mutations of Saccharomyces cerevisiae. Chromosoma. 1978 Dec 21;70(1):109–130. [PubMed]
  • Silva-Lopez E, Zamb TJ, Roth R. Role of premeiotic replication in gene conversion. Nature. 1975 Jan 17;253(5488):212–214. [PubMed]
  • Simchen G, Hirschberg J. Effects of the mitotic cell-cycle mutation cdc4 on yeast meiosis. Genetics. 1977 May;86(1):57–72. [PubMed]
  • Trew BJ, Friesen JD, Moens PB. Two-dimensional protein patterns during growth and sporulation in Saccharomyces cerevisiae. J Bacteriol. 1979 Apr;138(1):60–69. [PMC free article] [PubMed]
  • Warren AJ, Warren WD, Cox EC. Genetic and morphological study of aggregation in the cellular slime mold Polysphondylium violaceum. Genetics. 1976 May;83(1):25–47. [PubMed]

Articles from Molecular and Cellular Biology are provided here courtesy of American Society for Microbiology (ASM)