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Science. Author manuscript; available in PMC 2013 June 27.
Published in final edited form as:
PMCID: PMC3694778
NIHMSID: NIHMS483291

Crystal Structure of the Heterodimeric CLOCK:BMAL1 Transcriptional Activator Complex

Abstract

The circadian clock in mammals is driven by an autoregulatory transcriptional feedback mechanism that takes about 24 hours to complete. A key component of this mechanism is a heterodimeric transcriptional activator consisting of two bHLH-PAS domain protein subunits, CLOCK and BMAL1. Here we report the crystal structure of a complex containing the mouse CLOCK:BMAL1 bHLH-PAS domains at 2.3Å resolution. The structure reveals an unusual asymmetric heterodimer with the three domains in each of the two subunits, bHLH, PAS-A and PAS-B tightly intertwined and involved in dimerization interactions, resulting in three distinct protein interfaces. Mutations that perturb the observed heterodimer interfaces affect the stability and activity of the CLOCK:BMAL1 complex as well as the periodicity of the circadian oscillator. The structure of the CLOCK:BMAL1 complex is a starting point for understanding at an atomic level the mechanism driving the mammalian circadian clock.

The basic helix-loop-helix PER-ARNT-SIM (bHLH-PAS) proteins, CLOCK and BMAL1 (ARNTL), are the primary transcriptional activators within the circadian clock mechanism of mammals. Since the molecular identification of the Clock gene fifteen years ago (1, 2), the transcriptional network that drives circadian oscillations has been systematically identified (35). CLOCK and BMAL1 heterodimerize and interact with E-box regulatory elements in the Period (Per1, Per2) and Cryptochrome (Cry1, Cry2) genes to activate their transcription during the daytime (6, 7). Their protein products, PER and CRY, accumulate, dimerize and translocate into the nucleus at night where they interact directly with CLOCK:BMAL1 to repress their own transcription (710). As the PER:CRY repressor complex is degraded by specific E3 ubiquitin ligase complexes (1114), repression is relieved, and CLOCK:BMAL1 then activate a new round of transcription to begin the circadian cycle anew. This cell-autonomous, autoregulatory transcriptional feedback loop takes approximately 24 hours to complete and forms the core mechanism of the circadian clock in mammals (5).

CLOCK and BMAL1 belong to a family of transcriptional regulators that contain bHLH and PAS domains. In mammals, these bHLH-PAS transcription factors participate in a wide array of functions including responses to environmental contaminants (Aryl hydrocarbon receptor, AHR), hypoxia (Hypoxia inducible factor, HIF), neurogenesis (SIM1), synaptic plasticity (NPAS4) and circadian regulation (CLOCK, NPAS2, BMAL1) (15) and most of them remain poorly characterized at the structural level. By contrast, the structures of individual PAS domains and their interactions with small molecule ligands such as heme and flavin cofactors are well understood, especially among microorganisms and plants, where PAS domains serve important roles in two-component signaling and blue-light detection (16, 17). Although the PAS fold is widely conserved, it has intrinsic flexibility and can adapt to different conformations depending on bound ligands or interacting partners (16). Here we present the three-dimensional structure of the bHLH-PAS domains from the mouse CLOCK:BMAL1 heterodimer at 2.3 Å resolution.

Overall structure of CLOCK:BMAL1

To obtain stable CLOCK:BMAL1 complexes suitable for crystallographic analysis, we used protein constructs containing the bHLH and the two tandem PAS-A and PAS-B domains (Fig. 1A). N-terminal His tagged mouse CLOCK (residues 26–384) and native mouse BMAL1 (residues 62–447) constructs were co-expressed in Sf9 insect cells and co-purified (See Supplementary Materials and Methods). To confirm that that the resulting heterodimeric protein binds DNA, we assayed the affinity of binding to oligonucleotides containing the canonical E-box sequence (CACGTG) from the mPer1 and mPer2 promoters and observed Kd’s of ~10 nM (Fig. 1B, S1). Crystals of CLOCK:BMAL1 were obtained that diffracted to 2.3Å at synchrotron sources. The phases of CLOCK:BMAL1 were determined by the single wavelength anomalous dispersion (SAD) method using selenomethionine-labeled CLOCK:BMAL1 crystals (Fig. S2). Data collection and refinement statistics are shown in Supplementary Table S1.

Fig. 1
Overall structure of mouse CLOCK:BMAL1. (A) Domain organization of CLOCK and BMAL1. Crystals were obtained from the truncated proteins (indicated by the amino acid residue number) encompassing the bHLH-PAS-AB domains. (B) DNA-binding affinity of the truncated ...

The three-dimensional structure of CLOCK:BMAL1 reveals a tightly intertwined heterodimer (Fig. 1C, center) with all three domains, the N-terminal bHLH domain and two tandem PAS domains (PAS-A and PAS-B), involved in dimerization interactions. Each domain interacts primarily with the corresponding domain of its partner subunit so that CLOCK bHLH interacts with BMAL1 bHLH, and CLOCK PAS-A (or PAS-B) with BMAL1 PAS-A (or PAS-B). Although the primary sequences of these three domains are similar in CLOCK and BMAL1 (Fig. S3, S4), the spatial arrangement of these domains with respect to one another is strikingly different in the two subunits (Fig. 1C). In BMAL1, the second helix of the bHLH domain (α2) is nearly continuous with the N-terminal flanking helix (A′α) of the PAS-A domain despite insertion of a ~15 residue flexible loop (L1) (Fig. 1C, right panel). By contrast, in CLOCK there is a ~23Å displacement between the end of α2 and the beginning of the PAS-A A′α helix (Fig. 1C, left panel). As a consequence, the CLOCK PAS-A domain is in direct contact with the α2 helix of its bHLH domain while there are no direct contacts between the BMAL1 PAS-A and bHLH domains.

The asymmetry of the CLOCK:BMAL1 complex is also reflected in the divergent electrostatic potential distributions on the two subunits. The BMAL1 subunit has an overall positive electrostatic potential with a pI of 9.01 (and 8.55 for the PAS-A/B domains). The CLOCK subunit, on the other hand, has an overall negative electrostatic potential, with a pI of 5.86 for the bHLH-PAS domains (and 5.28 for the PAS-A/B domain only). In the three-dimensional CLOCK:BMAL1 complex structure, the exposed CLOCK PAS domain surfaces have a largely negative electrostatic potential, whereas the exposed BMAL1 PAS domains are mostly positively charged or neutral (Fig. 1D). These electrostatic features of the CLOCK:BMAL1 heterodimer produce an interesting dichotomy in the potential interaction interfaces of the complex and are consistent with prior work suggesting that the PER1, PER2, CRY1 and CRY2 proteins differentially interact with CLOCK and BMAL1 (8, 1820).

The PAS-A domains

While all three domains of the CLOCK and BMAL1 subunits are involved in intermolecular interactions, the heterodimeric interfaces between the individual PAS domains are particularly interesting. The CLOCK:BMAL1 heterodimer has long flexible loops interspersed with canonical PAS secondary structure elements in both PAS-A domains. In the BMAL1 PAS-A domain, a total of ~60 residues in three loop regions are disordered in the crystal structure, whereas in the CLOCK PAS-A domain, a single loop of ~25 residues is disordered. In spite of this high degree of flexibility, the two PAS-A domains adopt a typical PAS fold (Fig. 2A) and are structurally similar to each other with a root mean square deviation (rmsd) of only 0.62Å over 79 Cα atoms. As seen in typical PAS domains, the core of CLOCK and BMAL1 PAS-A domains contains a five-stranded antiparallel β sheet (AβBβGβHβIβ) and several α helices (Cα, DαEαFα) flanking the concave surface of the sheet. In contrast to the PAS-B domain (Fig. 2A), both PAS-A domains in the two subunits contain an N-terminal flanking helix (A′α) external to the canonical PAS-domain fold. The A′α helices of the CLOCK and BMAL1 PAS-A domains pack in between the β-sheet faces of the two domains to mediate the heterodimeric PAS-A interactions (Fig. 2B). The A′αhelix of CLOCK PAS-A makes extensive contacts with the β-sheet face of BMAL1 PAS-A, while the A′αhelix of BMAL1 interacts with the β-sheet face of CLOCK PAS-A (Fig. 2B). This domain-swapped helical interface is reminiscent of a number of other PAS domain proteins, such as the N-terminal PAS domain of the nitrogen fixation regulatory protein NifL from Azotobacter vinelandii (21) (Fig. 2C) and the N-terminal domain of the transcriptional factor TyrR from E. coli (22).

Fig. 2
Structure and interaction of the PAS-A domains of CLOCK:BMAL1. (A) Ribbon representations of CLOCK PAS-A domain. Secondary structures are color ramped from blue to red and labeled from the A′α helix located N-terminal to the canonical ...

The CLOCK:BMAL1 PAS-A dimer interface is largely mediated by conserved hydrophobic interactions. Specifically, Phe104, Leu105, and Leu113 on the A′α helix of CLOCK (corresponding residues on BMAL1 PAS-A are Phe141, Leu142 and Leu150) contact a hydrophobic region on the β-sheet face of BMAL1 PAS-A comprised of residues Leu159 on strand Aβ, Thr285 and Tyr287 on Hβ, Val315 and Ile317 on strand Iβ (Fig. 2B, left). A similar interface can be found between the A′α helix of BMAL1 and CLOCK PAS-A domain (Fig. 2B, right). As a result, the two PAS-A domains in CLOCK:BMAL1 form a parallel dimer related by an approximate two-fold symmetry, with an extensive buried surface area (~1950 Å2) and topologically complex interface between the two subunits. Many of the residues observed in the CLOCK:BMAL1 PAS-A dimer interface are conserved among bHLH-PAS transcription factors (Fig. S4), suggesting that these proteins may share a common PAS-A domain dimerization mode.

The PAS-B domains

A ~15 residue linker (L2) connects the PAS-A and PAS-B domains in each of the CLOCK and BMAL1 subunits, though the linker conformation and the relative spatial arrangement of the two PAS domains in the two subunits are different (Fig. 1C). In CLOCK, a large portion of L2 is buried between the interface of CLOCK and BMAL1 and is well ordered (Fig. 1C). By contrast, in BMAL1, L2 between PAS-A and PAS-B is solvent exposed and very flexible as indicated by high atomic displacement parameters (B-factors). The PAS-B domains of CLOCK and BMAL1 are related predominantly by a ~26Å translation and are stacked in a roughly parallel fashion (Fig. 3A), different from the antiparallel β-sheet interface seen for the isolated PAS-B domain complex of HIF-2α:ARNT (Fig. 3B) (23). The β sheet of BMAL1 PAS-B makes contacts with the helical face of CLOCK PAS-B, burying a patch of hydrophobic residues on both subunits. Several surface-exposed hydrophobic residues on both CLOCK and BMALl PAS-B become mostly or partially buried upon dimerization, including Tyr310, Val315, Leu318 of CLOCK and Phe423, Trp427 and Val435 of BMAL1, resulting in ~700 Å2 of buried surface area (Fig. 3C, 3E). Most prominently within these hydrophobic interactions, BMAL1 Trp427, located on the short HI loop (connecting the Hβ and Iβ strands), intrudes into a hydrophobic cleft created between the Fα helix and the AB loop of CLOCK PAS-B (Fig. 3D, 3F), and partially stacks against the indole ring of CLOCK Trp284.

Fig. 3
Interface between CLOCK:BMAL1 PAS-B domains. (A) The spatial arrangement of the two PAS-B domains in CLOCK:BMAL1. (B) Antiparallel orientation of β sheet-mediated interaction between isolated HIF-2α:ARNT PAS-B domains (pdb: 3F1P). (C) ...

CLOCK:BMAL1 heterodimer conformation and transactivation function

To probe the relationship between the observed conformation of the CLOCK:BMAL1 heterodimer and its function, we generated a series of mutations predicted to perturb the interfaces between each of the three domains (Fig. 4A). For the bHLH domains, the C-terminal halves of the α1 helices, together with the α2 helices of both CLOCK and BMAL1 participate in the formation of a canonical four-helical bHLH bundle in the heterodimer similar to that observed in USF1 and MYC:MAX (2426) (Fig. S3A). As seen in other bHLH proteins, the core of this four-helical bundle is highly hydrophobic (26, 27), indicating that dimerization of the bHLH domains should help stabilize the CLOCK:BMAL1 complex. The proper conformation of the bHLH domain is also critical for E-box DNA recognition as the DNA binding α1 helices need to be positioned precisely to interact with the major groove sites of the E-box DNA duplex (24, 26, 27). Indeed, when the bHLH hydrophobic core residues Leu57 and Leu74 of CLOCK, and Leu95 and Leu115 of BMAL1 are mutated to glutamate, the transactivation activity of these full-length CLOCK:BMAL1 mutants are eliminated, as demonstrated by measuring E-box-driven luciferase reporter gene activity in human kidney cells (HEK293T) with transiently transfected CLOCK and BMAL1 mutant proteins (Fig. 4B).

Fig. 4
Functional analysis of CLOCK:BMAL1 mutants. (A) Locations of domain interface mutants in CLOCK (green) and BMAL1 (blue). (B) Per2 promoter:Luciferase reporter assays to evaluate the effects of structure-based mutations on transactivation by full-length ...

We examined the dimerization of these mutants in living cells through a bimolecular fluorescence complementation (BiFC) assay, in which the N- and C-terminal fragments of the fluorescent protein Venus (Ven-N and Ven-C) were fused to the C-termini of truncated bHLH-PAS domain constructs of CLOCK and BMAL1, respectively (See Supplementary Online Material). Dimerization of CLOCK and BMAL1 brings the two Venus fragments into close proximity to facilitate the formation of an intact fluorescent protein, thus providing a fluorescent readout for protein-protein interactions in living cells (28). The BiFC data showed that mutation at the bHLH hydrophobic core reduced formation of a stable heterodimeric complex (Fig. 4C, Fig. S5). Furthermore, three of these bHLH domain mutations, CLOCK L74E (C:L74E), BMAL1 L95E and L115E (B:L95E and B:L115E) also destabilized the full-length CLOCK:BMAL1 heterodimer as shown by co-immunoprecipitation (co-IP) assays (Fig 4D).

Next, we mutated residues involved in PAS-A and PAS-B domain interfaces to test their effects on transactivation activity and CLOCK:BMAL1 heterodimer formation. To examine the PAS-A domain interface, we made following mutations: L113E (on A′α) and F122D (on Aβ of CLOCK, and L150E (on A′α) and I317D (on Iβ of BMAL1, and performed transactivation, BiFC, and co-IP assays (Fig. 4). Single mutations of C:L113E, C:F122D or B:L150E were not sufficient to reduce dimerization or transactivation activity (Fig. 4B–D). However, BMAL1 mutant I317D had decreased transcriptional activity (~80% of wild type control) (Fig. 4B) and decreased affinity for CLOCK as demonstrated by the BiFC and co-IP experiments (Fig. 4C, 4D, SOM Fig. S5). Furthermore, when opposing CLOCK and BMAL1 PAS-A domain interface residues were doubly mutated as in C:L113E+B:I317D, the association between full-length CLOCK and BMAL1 subunits was not detectable under the assay conditions and transactivation activity was reduced to ~25% of the control (Fig. 4B–D).

To examine the unusual interface between the PAS-B domains of the CLOCK:BMAL1 heterodimer, we made the following PAS-B domain mutations: W284A and V315R on the CLOCK helical face, and W427A, F423R, and V435R on the β-sheet face of BMAL1. Single mutations in either CLOCK or BMAL1 PAS-B domains had a limited effect on the transactivation activity by the full-length mutant protein (Fig. 4B), although the activities of C:W284A, C:V315R and B:F423R were reduced by ~20–30% compared to the wild-type (WT) protein (Fig. 4B). Additionally, the BiFC signal of mutants C:W284A, C:V315R and B:V435R decreased dramatically compared to the WT protein (Fig. 4C, SOM Fig. S5), indicating that the PAS-B domain interactions of these mutants may be altered. The effect of these single mutations on the interactions of the full-length proteins, as measured by co-IP, was more subtle, with partially weakened interactions for mutants C:W284A and B:W427A (Fig 4D). Notably, the double BMAL1 PAS-B domain mutant, B:F423R/V435R and the combined CLOCK:BMAL1 mutant C:W284A+B:W427A showed a decreased interaction in both co-IP and BiFC assays, as well as a reduction in transactivation activity (Fig. 4B–D). These data support the unusual PAS-B domain interface observed in the crystal structure involving the helical face of CLOCK and the β-sheet face of BMAL1, and specifically indicate that contact between CLOCK Trp284 and BMAL1 Trp427 is important for PAS-B interaction.

CLOCK:BMAL1 mutants alter circadian cycling in cells

To examine the functional consequences of mutations that compromise CLOCK:BMAL1 heterodimer formation and transactivation potential, we assessed circadian rhythmns in mouse Per2Luc fibroblasts overexpressing mutant CLOCK or BMAL1 constructs introduced by lentiviral vectors (see Supplemental Materials and Methods) (SOM Fig. S6). On the basis of in vivo transgenic experiments, we can infer that CLOCK levels are rate limiting and that overexpression of CLOCK leads to a shortening of circadian period in both constitutively expressed or conditionally expressed transgenic mice (2, 29). In contrast, overexpression of BMAL1 can have no effect or can lengthen circadian period (30), and these effects of BMAL1 overexpression are consistent with the hypothesis that BMAL1 is normally in excess of CLOCK. Higher overexpression of BMAL1 can lead to period lengthening, possibly by sequestering of CLOCK via a squelching mechanism (31). Thus, we can assay the WT function of CLOCK and BMAL1 by overexpression in PER2::luciferase cycling cell assays (32), and by extension, infer loss-of-function mutations by their inability to mimic WT function, or in contrast, dominant-negative mutations by their disruption of normal rhythms.

Control Per2Luc fibroblasts overexpressing GFP had robust luciferase rhythms with a period of 23.1 hours (SOM Fig. S6). Cells overexpressing WT CLOCK or BMAL1 exhibited rhythms with either shorter (~22.0 hr) or longer (24.6 hr) periods, respectively (SOM Fig. S6A). Both the CLOCK mutants tested (C:L57E and C:W284A) failed to mimic WT CLOCK and had period values similar to the GFP control cells (~23 hr), and therefore behaved as loss-of-function mutations. Notably, the C:L57E mutant abolished transactivation by full-length CLOCK:BMAL1 and reduced dimerization of the truncated heterodimer (Fig. 4B, 4C). Although the C:W284A PAS-B mutant had only a 20% reduction in activty in transactivation assays (Fig. 4B), it weakened CLOCK:BMAL1 dimerization significantly (Fig. 4C–D) and failed to mimic the WT function of CLOCK on circadian periodicity. Interestingly, overexpression of BMAL1 mutants within the bHLH and PAS-A domains had different effects on the period of circadian rhythms (SOM Fig. S6). Overexpression of the bHLH mutant B:L95E led initially to a longer period (25.1 hrs) for the first 3 days followed by disruption of circadian rhythmicity, whereas overexpression of the PAS-A mutant B:I317D led to a shorter period (~23.8 hr) compared to the cells overexpressing WT BMAL1 (24.6 hrs) and thus behaved as a partial loss-of-function mutation.

Discussion

Here we present the X-ray structure of the mouse CLOCK:BMAL1 transcriptional activator complex, a central regulator in the circadian clock. With the CLOCK:BMAL1 complex structure in hand, it will now be possible to analyze the multiprotein complexes involved in mammalian circadian clock mechanisms at an atomic level. Existing genetic and biochemical data indicate that the negative regulators CRY and PER physically interact with CLOCK:BMAL1 to form the major repressive clock complex containing CLOCK:BMAL1 and PER:CRY (9, 10, 18, 33). Although the structural details of these interactions have not been elucidated, the binding of CRY and/or PER to CLOCK:BMAL1 could affect DNA-binding, modulate transactivation potential, or modify interactions with co-activators and co-repressors. Previous work suggests that CRY interacts with the PAS-B domain of CLOCK near its β-sheet face and also with a C-terminal region of BMAL1 (18, 19, 33). Specifically, mutations of residues Gln332, His360, Gln361, Trp362 and Glu367 of the CLOCK PAS-B domain interfere with repression by CRY. In the crystal structure, these residues are located on the HI loop of the solvent exposed β-sheet face of the CLOCK PAS-B domain fully accessible for interaction with CRY (Fig. 5). The electrostatic distribution of CLOCK PAS domains is also consistent with the idea that CLOCK is the site for CRY binding because CRY is a highly positively charge protein (pI = 8.24 for CRY1) and would complement the negative surface charge on CLOCK (Fig 1D). Thus, the unusual spatial arrangement of the PAS-B domains of CLOCK:BMAL1 observed in the crystal structure is consistent with the earlier biochemical data on the PAS-B domain function. Interestingly the tandem PAS domains in BMAL1 have a spatial arrangement similar to that observed in crystal structures of the mouse and Drosophila PER tandem PAS domains (Fig. S7) (3437). This suggests that the tandem PAS domains in BMAL1 and PER may have a deeper degree of structural and/or functional conservation than was previously appreciated, which may have implications for how the PAS-A and PAS-B domains of PER2 interact with either CLOCK or BMAL1 (10).

Fig. 5
Mutations that reduce repression of CLOCK:BMAL1 transactivation by CRY localize to CLOCK PAS-B HI loop. CRY-derepressing mutations arising from a random mutagenesis screen: G332E, H360Y, E367K (18) or directed mutagenesis study, Q361P/W362R (19) are predominantly ...

Interestingly, Trp362 of CLOCK, implicated in an interaction with CRY, corresponds to Trp427 of BMAL1, which was shown here to interact with CLOCK (Fig. 3D, 3F). Moreover, a tryptophan residue at the same position is also conserved in the Drosophila and mouse PER proteins (i.e., Trp482 of dPER and Trp419 of mPER2), and they are shown in the crystal structures to be involved in the interaction with a second PER protein to form homodimers (35). These observations highlight a potentially conserved functional role for the tryptophan residue located at the HI loop of the PAS-B domains of these clock proteins.

The CLOCK:BMAL1 PAS-B domain interface reveals details of a mode of PAS protein-protein interaction involving the α-helical face of CLOCK PAS-B and the β-sheet face of BMAL1 PAS-B (Fig. 3C–3F and Fig S8A). Notably, the same region (between Fα and the AB loop) on the helical face of PER PAS-B is utilized for intramolecular interactions with a C-terminal α helix (αE) containing nuclear exporting signal (NES) residues (37) (Note: helix αE is equivalent to Jα in canonical PAS nomenclature.) (Fig. S8B). Similarly, ARNT has been shown by NMR studies to use the same helical region for interacting with a family of helical coactivator proteins that are required for transactivation by the heterodimeric HIF complex (38, 39). Moreover, the same region of many bacterial and plant PAS proteins binds to small molecule ligands such as flavin cofactors, flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) (16) (Fig. S8C). Overall, these data highlight the remarkable structural plasticity and adaptability of PAS domains. Because CLOCK:BMAL1 is a prototypical bHLH-PAS protein family member, the structural features of the CLOCK:BMAL1 complex may be shared by other bHLH-PAS proteins. It will be important in future work to determine the structures of additional heterodimeric bHLH-PAS proteins such as HIF:ARNT and AHR:ARNT and observe the structural basis by which these homologous proteins confer their distinct and pathway specific functions.

In summary, the structure of CLOCK:BMAL1 has revealed the locations of previously identified sites on these proteins that affect their inhibition by CRY. It has also revealed an unexpected similarity in the orientation of the tandem PAS-A and PAS-B domains of BMAL1 to that found in the PERIOD proteins. These observations provide a starting point for the determination of how the CRY and PER proteins interact with and repress CLOCK:BMAL1, which in turn should yield insight into the detailed biochemical mechanism by which this transcriptional feedback loop drives the circadian clock.

Supplementary Material

Supplementary Online Material

Acknowledgments

This work was supported by the Howard Hughes Medical Institute (J.S.T.), an American Heart Association grant 10GRNT4310090 (H.Z.), and NIH R01 GM090247 (C.B.G.). We thank N. Grishin and K. Gardner for helpful discussions; S. Padrick for help with the fluorescence polarization assay; M. Rosen for use of his fluorometer; D. Tomchick and H. Aronovich for technical assistance; and C. Ralston, L. Steinhour and C. Brautigan for help with data collection. The Berkeley Center for Structural Biology is supported in part by the National Institutes of Health, National Institute of General Medical Sciences, and the Howard Hughes Medical Institute. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. J.S.T. is an Investigator, Y.C. is a Research Specialist 3 and C.P. was an Associate in the Howard Hughes Medical Institute.

Footnotes

Atomic coordinates for the reported crystal structures have been deposited with the Protein Data Bank under accession code 4F3L.

References and Notes

1. King DP, et al. Cell. 1997;89:641. [PubMed]
2. Antoch MP, et al. Cell. 1997;89:655. [PubMed]
3. Reppert SM, Weaver DR. Nature. 2002;418:935. [PubMed]
4. Lowrey PL, Takahashi JS. Annu Rev Genom Hum Genet. 2004;5:407. [PubMed]
5. Lowrey PL, Takahashi JS. Adv Genet. 2011;74:175. [PubMed]
6. Gekakis N, et al. Science. 1998;280:1564. [PubMed]
7. Kume K, et al. Cell. 1999;98:193. [PubMed]
8. Griffin EA, Jr, Staknis D, Weitz CJ. Science. 1999;286:768. [PubMed]
9. Lee C, Etchegaray JP, Cagampang FR, Loudon AS, Reppert SM. Cell. 2001;107:855. [PubMed]
10. Chen R, et al. Mol Cell. 2009;36:417. [PMC free article] [PubMed]
11. Shirogane T, Jin J, Ang XL, Harper JW. J Biol Chem. 2005;280:26863. [PubMed]
12. Reischl S, et al. J Biol Rhythms. 2007;22:375. [PubMed]
13. Busino L, et al. Science. 2007;316:900. [PubMed]
14. Siepka SM, et al. Cell. 2007;129:1011. [PubMed]
15. McIntosh BE, Hogenesch JB, Bradfield CA. Annu Rev Physiol. 2010;72:625. [PubMed]
16. Möglich A, Ayers RA, Moffat K. Structure. 2009;17:1282. [PMC free article] [PubMed]
17. Henry JT, Crosson S. Annu Rev Microbiol. 2011;65:261. [PMC free article] [PubMed]
18. Sato TK, et al. Nat Genet. 2006;38:312. [PMC free article] [PubMed]
19. Zhao WN, et al. Nature cell biology. 2007;9:268. [PubMed]
20. Ye R, Selby CP, Ozturk N, Annayev Y, Sancar A. J Biol Chem. 2011;286:25891. [PMC free article] [PubMed]
21. Key J, Hefti M, Purcell EB, Moffat K. Biochemistry. 2007;46:3614. [PubMed]
22. Verger D, Carr PD, Kwok T, Ollis DL. J Mol Biol. 2007;367:102. [PubMed]
23. Scheuermann TH, et al. Proc Natl Acad Sci USA. 2009;106:450. [PubMed]
24. Ferré-D’ Amaré AR, Prendergast GC, Ziff EB, Burley SK. Nature. 1993;363:38. [PubMed]
25. Ferré-D’ Amaré AR, Pognonec P, Roeder RG, Burley SK. EMBO J. 1994;13:180. [PubMed]
26. Nair SK, Burley SK. Cell. 2003;112:193. [PubMed]
27. Ma PC, Rould MA, Weintraub H, Pabo CO. Cell. 1994;77:451. [PubMed]
28. Kerppola TK. Nat Rev Mol Cell Biol. 2006;7:449. [PMC free article] [PubMed]
29. Hong HK, et al. PLoS Genet. 2007;3:e33. [PMC free article] [PubMed]
30. McDearmon EL, et al. Science. 2006;314:1304. [PubMed]
31. Ptashne M. Nature. 1988;335:683. [PubMed]
32. Yoo SH, et al. Proc Natl Acad Sci USA. 2004;101:5339. [PubMed]
33. Kiyohara YB, et al. Proc Natl Acad Sci USA. 2006;103:10074. [PubMed]
34. Yildiz O, et al. Mol Cell. 2005;17:69. [PubMed]
35. Hennig S, et al. PLoS Biol. 2009;7:e94. [PMC free article] [PubMed]
36. King HA, Hoelz A, Crane BR, Young MW. J Mol Biol. 2011;413:561. [PMC free article] [PubMed]
37. Kucera N, et al. Proc Natl Acad Sci USA. 2012;109:3311. [PubMed]
38. Partch CL, Card PB, Amezcua CA, Gardner KH. J Biol Chem. 2009;284:15184. [PubMed]
39. Partch CL, Gardner KH. Proc Natl Acad Sci USA. 2011;108:7739. [PubMed]
40. Yoo SH, et al. Proc Natl Acad Sci USA. 2005;102:2608. [PubMed]
41. Cardone L, et al. Science (New York, NY) 2005;309:1390. [PubMed]
42. Tamaru T, et al. Nature Structural & Molecular Biology. 2009;16:446. [PubMed]
43. Yoshitane H, et al. Mol Cell Biol. 2009;29:3675. [PMC free article] [PubMed]
44. Zhou T, et al. Structure. 2004;12:1891. [PubMed]
45. Otwinowski Z, Minor W. Method Enzymol. 1997;276:307.
46. Abrahams JP, Leslie AG. Acta Crystallogr D Biol Crystallogr. 1996;52:30. [PubMed]
47. Sheldrick GM. Acta Crystallogr A. 2008;64:112. [PubMed]
48. Vonrhein C, Blanc E, Roversi P, Bricogne G. Methods Mol Biol. 2007;364:215. [PubMed]
49. Perrakis A, Morris R, Lamzin VS. Nat Struct Biol. 1999;6:458. [PubMed]
50. Cowtan K. Acta Crystallogr D Biol Crystallogr. 2006;62:1002. [PubMed]
51. Emsley P, Lohkamp B, Scott WG, Cowtan K. Acta Cryst. 2010;66:486. [PMC free article] [PubMed]
52. N. Collaborative Computational Project. Acta Crystallogr D Biol Crystallogr. 1994;50:760. [PubMed]
53. Murshudov GN, Vagin AA, Dodson EJ. Acta Crystallogr D Biol Crystallogr. 1997;53:240. [PubMed]
54. Adams PD, et al. Acta Crystallogr D Biol Crystallogr. 2010;66:213. [PMC free article] [PubMed]
55. Baker NA. Proceedings of the National Academy of Sciences. 2001;98:10037. [PubMed]
56. Anderson BJ, Larkin C, Guja K, Schildbach JF. Methods Enzymol. 2008;450:253. [PMC free article] [PubMed]
57. Hida A, et al. Genomics. 2000;65:224. [PubMed]
58. Wang ZX. FEBS Lett. 1995;360:111. [PubMed]
59. Liu H, Naismith JH. BMC Biotechnol. 2008;8:91. [PMC free article] [PubMed]
60. Hu CD, Chinenov Y, Kerppola TK. Mol Cell. 2002;9:789. [PubMed]
61. Hu CD, Kerppola TK. Nat Biotechnol. 2003;21:539. [PMC free article] [PubMed]
62. Meyer P, Saez L, Young MW. Science. 2006;311:226. [PubMed]
63. Li Y, Yu W, Liang Y, Zhu X. Cell Res. 2007;17:701. [PubMed]
64. Wang F, Zhang Q, Cao J, Huang Q, Zhu X. Exp Cell Res. 2008;314:213. [PubMed]
65. Welsh DK, Yoo SH, Liu AC, Takahashi JS, Kay SA. Curr Biol. 2004;14:2289. [PubMed]
66. Buhr ED, Yoo SH, Takahashi JS. Science. 2010;330:379. [PMC free article] [PubMed]
67. Chen VB, et al. Acta Crystallogr D Biol Crystallogr. 2009;66:12. [PMC free article] [PubMed]