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Mol Cell Biol. 1981 May; 1(5): 469–474.
PMCID: PMC369342

SUC1 gene of Saccharomyces: a structural gene for the large (glycoprotein) and small (carbohydrate-free) forms of invertase.


Saccharomyces cerevisiae revertant strain D10-ER1 has been shown to contain thermosensitive forms of the large (glycoprotein) and small (carbohydrate-free) invertases and a very low level of the small enzyme, along with a wild-type level of the large form (T. Mizunaga et al., Mol. Cell. Biol. 1:460-468, 1981). These characteristics cosegregated in crosses of the revertant strain with wild-type sucrose-fermenting (SUC1) or nonfermenting (suc0) strains. In addition, there is tight linkage between sucrose and maltose fermentation in revertant D10-ER1 (characteristic of the SUC1 and MAL1 genes). From this we infer that a single reversion event is responsible for the several changes observed in D10-ER1, and that this mutation maps within or very close to the SUC1 gene present in the ancestor strain 4059-358D. The revertant SUC1 allele in D10-ER1 (termed SUC1-R1) was expressed independently of the wild-type SUC1 gene when both were present in diploid cells. Diploids carrying only the wild-type or the mutant genes synthesized invertases with the characteristics of the parental Suc+ haploids. The possibility that a modifier gene was responsible for the alterations in the invertases of revertant D10-ER1 was ruled out by appropriate crosses. We conclude that SUC1 is a structural gene that codes for both the large and the small forms of invertase and suggest that SUC2 through SUC5 are structural genes as well.

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Selected References

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  • Abrams BB, Hackel R, Mizunaga T, Lampen JO. Relationship of large and small invertases in Saccharomyces: mutant selectively deficient in small invertase. J Bacteriol. 1978 Sep;135(3):809–817. [PMC free article] [PubMed]
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  • Grossmann MK, Zimmermann FK. The structural genes of internal invertases in Saccharomyces cerevisiae. Mol Gen Genet. 1979 Sep;175(2):223–229. [PubMed]
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  • Mizunaga T, Tkacz JS, Rodriguez L, Hackel RA, Lampen JO. Temperature-sensitive forms of large and small invertase in a mutant derived from a SUC1 strain of Saccharomyces cerevisiae. Mol Cell Biol. 1981 May;1(5):460–468. [PMC free article] [PubMed]
  • Mortimer RK, Hawthorne DC. Genetic mapping in Saccharomyces. Genetics. 1966 Jan;53(1):165–173. [PubMed]
  • Ottolenghi P. Some properties of five non-allelic -D-fructofuranosidases (invertases) of Saccharomyces. C R Trav Lab Carlsberg. 1971;38(13):213–221. [PubMed]

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