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Mol Cell Biol. 1984 May; 4(5): 985–988.
PMCID: PMC368855

5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA.

Abstract

We examined the DNA of Saccharomyces cerevisiae by both HpaII-MspI restriction enzyme digestion and high-performance liquid chromatography analysis for the possible presence of 5-methylcytosine. Both of these methods failed to detect cytosine methylation within this yeast DNA; i.e., there is less than 1 5-methylcytosine per 3,100 to 6,000 cytosine residues.

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