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Clin Diagn Lab Immunol. 1994 January; 1(1): 44–50.
PMCID: PMC368194

Detection of borreliacidal antibody by using acridine orange and flow cytometry.

Abstract

Borreliacidal antibody has been shown to be important for the serodiagnosis of Lyme disease and determination of immune status. Our results show that borreliacidal antibody can be rapidly and accurately detected by flow cytometry. Acridine orange was added to normal and immune sera containing Borrelia burgdorferi organisms in the presence and absence of complement prior to data acquisition by flow cytometry. The flow cytometric parameters of side scatter and detection of acridine orange fluorescence were used to determine events per minute (number of labeled spirochetes), percent shift in fluorescence (number of dead spirochetes), and mean channel fluorescence (intensity of fluorescence-labeled spirochetes) of acridine orange-labeled spirochetes. Borreliacidal antibody was detected as early as 4 h, with optimal detection 16 to 24 h after incubation of B. burgdorferi organisms with immune serum and complement. Our results also showed that complement was necessary for detection of borreliacidal antibody. Flow cytometry with acridine orange-labeled spirochetes provides a rapid means for detection of borreliacidal antibody.

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Selected References

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  • Bakken LL, Case KL, Callister SM, Bourdeau NJ, Schell RF. Performance of 45 laboratories participating in a proficiency testing program for Lyme disease serology. JAMA. 1992 Aug 19;268(7):891–895. [PubMed]
  • Barbour AG, Burgdorfer W, Grunwaldt E, Steere AC. Antibodies of patients with Lyme disease to components of the Ixodes dammini spirochete. J Clin Invest. 1983 Aug;72(2):504–515. [PMC free article] [PubMed]
  • Callister SM, Case KL, Agger WA, Schell RF, Johnson RC, Ellingson JL. Effects of bovine serum albumin on the ability of Barbour-Stoenner-Kelly medium to detect Borrelia burgdorferi. J Clin Microbiol. 1990 Feb;28(2):363–365. [PMC free article] [PubMed]
  • Callister SM, Schell RF, Case KL, Lovrich SD, Day SP. Characterization of the borreliacidal antibody response to Borrelia burgdorferi in humans: a serodiagnostic test. J Infect Dis. 1993 Jan;167(1):158–164. [PubMed]
  • Callister SM, Schell RF, Lovrich SD. Lyme disease assay which detects killed Borrelia burgdorferi. J Clin Microbiol. 1991 Sep;29(9):1773–1776. [PMC free article] [PubMed]
  • Coleman JL, Benach JL. Isolation of antigenic components from the Lyme disease spirochete: their role in early diagnosis. J Infect Dis. 1987 Apr;155(4):756–765. [PubMed]
  • Craft JE, Grodzicki RL, Steere AC. Antibody response in Lyme disease: evaluation of diagnostic tests. J Infect Dis. 1984 May;149(5):789–795. [PubMed]
  • Dressler F, Whalen JA, Reinhardt BN, Steere AC. Western blotting in the serodiagnosis of Lyme disease. J Infect Dis. 1993 Feb;167(2):392–400. [PubMed]
  • Grodzicki RL, Steere AC. Comparison of immunoblotting and indirect enzyme-linked immunosorbent assay using different antigen preparations for diagnosing early Lyme disease. J Infect Dis. 1988 Apr;157(4):790–797. [PubMed]
  • Hechemy KE, Harris HL, Wethers JA, Stevens RW, Stock BR, Reilly AA, Benach JL. Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi. J Clin Microbiol. 1989 Aug;27(8):1854–1858. [PMC free article] [PubMed]
  • Hedberg CW, Osterholm MT, MacDonald KL, White KE. An interlaboratory study of antibody to Borrelia burgdorferi. J Infect Dis. 1987 Jun;155(6):1325–1327. [PubMed]
  • Karlsson M, Möllegård I, Stiernstedt G, Wretlind B. Comparison of Western blot and enzyme-linked immunosorbent assay for diagnosis of Lyme borreliosis. Eur J Clin Microbiol Infect Dis. 1989 Oct;8(10):871–877. [PubMed]
  • Lane RS, Lennette ET, Madigan JE. Interlaboratory and intralaboratory comparisons of indirect immunofluorescence assays for serodiagnosis of Lyme disease. J Clin Microbiol. 1990 Aug;28(8):1774–1779. [PMC free article] [PubMed]
  • Lee DA, Hoidal JR, Clawson CC, Quie PG, Peterson PK. Phagocytosis by polymorphonuclear leukocytes of Staphylococcus aureus and Pseudomonas aeruginosa adherent to plastic, agar, or glass. J Immunol Methods. 1983 Sep 30;63(1):103–114. [PubMed]
  • Lovrich SD, Callister SM, Schmitz JL, Alder JD, Schell RF. Borreliacidal activity of sera from hamsters infected with the Lyme disease spirochete. Infect Immun. 1991 Aug;59(8):2522–2528. [PMC free article] [PubMed]
  • Luger SW, Krauss E. Serologic tests for Lyme disease. Interlaboratory variability. Arch Intern Med. 1990 Apr;150(4):761–763. [PubMed]
  • Magnarelli LA, Meegan JM, Anderson JF, Chappell WA. Comparison of an indirect fluorescent-antibody test with an enzyme-linked immunosorbent assay for serological studies of Lyme disease. J Clin Microbiol. 1984 Aug;20(2):181–184. [PMC free article] [PubMed]
  • Pantazis CG, Kniker WT. Assessment of blood leukocyte microbial killing by using a new fluorochrome microassay. J Reticuloendothel Soc. 1979 Aug;26(2):155–170. [PubMed]
  • Pennell DR, Wand PJ, Schell RF. Evaluation of a quantitative fluorescence immunoassay (FIAX) for detection of serum antibody to Borrelia burgdorferi. J Clin Microbiol. 1987 Nov;25(11):2218–2220. [PMC free article] [PubMed]
  • Peterson PK, Clawson CC, Lee DA, Garlich DJ, Quie PG, Johnson RC. Human phagocyte interactions with the Lyme disease spirochete. Infect Immun. 1984 Nov;46(2):608–611. [PMC free article] [PubMed]
  • Russell H, Sampson JS, Schmid GP, Wilkinson HW, Plikaytis B. Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease. J Infect Dis. 1984 Mar;149(3):465–470. [PubMed]
  • Sachsenmeier KF, Schell K, Morrissey LW, Pennell DR, West RM, Callister SM, Schell RF. Detection of borreliacidal antibodies in hamsters by using flow cytometry. J Clin Microbiol. 1992 Jun;30(6):1457–1461. [PMC free article] [PubMed]
  • Schmitz JL, Schell RF, Hejka AG, England DM. Passive immunization prevents induction of Lyme arthritis in LSH hamsters. Infect Immun. 1990 Jan;58(1):144–148. [PMC free article] [PubMed]
  • Steere AC, Grodzicki RL, Kornblatt AN, Craft JE, Barbour AG, Burgdorfer W, Schmid GP, Johnson E, Malawista SE. The spirochetal etiology of Lyme disease. N Engl J Med. 1983 Mar 31;308(13):733–740. [PubMed]
  • Wilske B, Schierz G, Preac-Mursic V, Weber K, Pfister HW, Einhäupl K. Serological diagnosis of erythema migrans disease and related disorders. Infection. 1984 Sep-Oct;12(5):331–337. [PubMed]

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