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Mol Cell Biol. 1986 April; 6(4): 1058–1064.
PMCID: PMC367615

RNase P activity in the mitochondria of Saccharomyces cerevisiae depends on both mitochondrion and nucleus-encoded components.

Abstract

A requisite step in the biosynthesis of tRNA is the removal of 5' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA. This mitochondrial RNase P was sensitive to both micrococcal nuclease and protease, demonstrating that it requires both a nucleic acid and protein for activity. The presence of RNase P activity in vitro directly correlated with the presence of a locus on yeast mitochondrial DNA previously shown by genetic and biochemical studies to be required for tRNA maturation. The product of the locus, the 9S RNA, and this newly described mitochondrial RNase P activity cofractionated, providing further evidence that the 9S RNA is the RNA component of yeast mitochondrial RNase P.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Akaboshi E, Guerrier-Takada C, Altman S. Veal heart ribonuclease P has an essential RNA component. Biochem Biophys Res Commun. 1980 Sep 30;96(2):831–837. [PubMed]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Christianson T, Rabinowitz M. Identification of multiple transcriptional initiation sites on the yeast mitochondrial genome by in vitro capping with guanylyltransferase. J Biol Chem. 1983 Nov 25;258(22):14025–14033. [PubMed]
  • Denhardt DT. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. [PubMed]
  • Doersen CJ, Guerrier-Takada C, Altman S, Attardi G. Characterization of an RNase P activity from HeLa cell mitochondria. Comparison with the cytosol RNase P activity. J Biol Chem. 1985 May 25;260(10):5942–5949. [PubMed]
  • Frontali L, Palleschi C, Francisci S. Transcripts of mitochondrial tRNA genes in Saccharomyces cerevisiae. Nucleic Acids Res. 1982 Nov 25;10(22):7283–7293. [PMC free article] [PubMed]
  • Gardiner K, Pace NR. RNase P of Bacillus subtilis has a RNA component. J Biol Chem. 1980 Aug 25;255(16):7507–7509. [PubMed]
  • Guerrier-Takada C, Gardiner K, Marsh T, Pace N, Altman S. The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme. Cell. 1983 Dec;35(3 Pt 2):849–857. [PubMed]
  • Kline L, Nishikawa S, Söll D. Partial purification of RNase P from Schizosaccharomyces pombe. J Biol Chem. 1981 May 25;256(10):5058–5063. [PubMed]
  • Kole R, Baer MF, Stark BC, Altman S. E. coli RNAase P has a required RNA component. Cell. 1980 Apr;19(4):881–887. [PubMed]
  • Martin NC, Miller DL, Underbrink K, Ming X. Structure of a precursor to the yeast mitochondrial tRNAMetf. Implications for the function of the tRNA synthesis locus. J Biol Chem. 1985 Feb 10;260(3):1479–1483. [PubMed]
  • Martin N, Rabinowitz M, Fukuhara H. Isoaccepting mitochondrial glutamyl-tRNA species transcribed from different regions of the mitochondrial genome of Saccharomyces cerevisiae. J Mol Biol. 1976 Mar 5;101(3):285–296. [PubMed]
  • Martin NC, Underbrink-Lyon K. A mitochondrial locus is necessary for the synthesis of mitochondrial tRNA in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4743–4747. [PubMed]
  • Merten S, Synenki RM, Locker J, Christianson T, Rabinowitz M. Processing of precursors of 21S ribosomal RNA from yeast mitochondria. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1417–1421. [PubMed]
  • Miller DL, Martin NC. Characterization of the yeast mitochondrial locus necessary for tRNA biosynthesis: DNA sequence analysis and identification of a new transcript. Cell. 1983 Oct;34(3):911–917. [PubMed]
  • Morimoto R, Locker J, Synenki RM, Rabinowitz M. Transcription, processing, and mapping of mitochondrial RNA from grande and petite yeast. J Biol Chem. 1979 Dec 25;254(24):12461–12470. [PubMed]
  • Osinga KA, De Vries E, Van der Horst GT, Tabak HF. Initiation of transcription in yeast mitochondria: analysis of origins of replication and of genes coding for a messenger RNA and a transfer RNA. Nucleic Acids Res. 1984 Feb 24;12(4):1889–1900. [PMC free article] [PubMed]
  • Palleschi C, Francisci S, Bianchi MM, Frontali L. Initiation of transcription of a mitochondrial tRNA gene cluster in S. cerevisiae. Nucleic Acids Res. 1984 Oct 11;12(19):7317–7326. [PMC free article] [PubMed]
  • Palleschi C, Francisci S, Zennaro E, Frontali L. Expression of the clustered mitochondrial tRNA genes in Saccharomyces cerevisiae: transcription and processing of transcripts. EMBO J. 1984 Jun;3(6):1389–1395. [PubMed]
  • Robertson HD, Altman S, Smith JD. Purification and properties of a specific Escherichia coli ribonuclease which cleaves a tyrosine transfer ribonucleic acid presursor. J Biol Chem. 1972 Aug 25;247(16):5243–5251. [PubMed]
  • Romaniuk PJ, Uhlenbeck OC. Joining of RNA molecules with RNA ligase. Methods Enzymol. 1983;100:52–59. [PubMed]
  • Underbrink-Lyon K, Miller DL, Ross NA, Fukuhara H, Martin NC. Characterization of a yeast mitochondrial locus necessary for tRNA biosynthesis. Deletion mapping and restriction mapping studies. Mol Gen Genet. 1983;191(3):512–518. [PubMed]
  • Zassenhaus HP, Martin NC, Butow RA. Origins of transcripts of the yeast mitochondrial var 1 gene. J Biol Chem. 1984 May 10;259(9):6019–6027. [PubMed]

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