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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Int Anesthesiol Clin. Author manuscript; available in PMC 2013 June 6.
Published in final edited form as:
PMCID: PMC3674880
NIHMSID: NIHMS475099

General Anesthetics and Molecular Mechanisms of Unconsciousness

Abstract

General anesthetic agents are unique in clinical medicine, because they are the only drugs used to produce unconsciousness as a therapeutic goal. In contrast to older hypotheses that assumed all general anesthetics produce their central nervous system effects through a common mechanism, we outline evidence that general anesthesia represents a number of distinct pharmacological effects that are likely mediated by different neural circuits, and perhaps via different molecular targets. Within the context of this neurobiological framework, we review recent molecular pharmacological and transgenic animal studies. These studies reveal that different groups of general anesthetics, which can be discerned based on their clinical features, produce unconsciousness via distinct molecular targets and therefore via distinct mechanisms. We further postulate that different types of general anesthetics selectively disrupt different critical steps (perhaps in different neuronal circuits) in the processing of sensory information and memory that results in consciousness.

Keywords: General anesthesia, Consciousness, Molecular pharmacology, Trangenic animals, Ligand-gated ion channels, GABA-A receptor, NMDA receptor, Inhaled anesthetics, Intravenous anesthetics

Introduction: Unconsciousness is the Sine Qua Non of General Anesthesia

The term “anesthesia” was originally used by the ancient Greek surgeon Dioscorides and resurrected by Dr. Oliver Wendell Holmes to describe the insensible state produced by inhalation of ether (1,2). The goals of general anesthesia include amnesia, unconsciousness (also termed hypnosis), and immobilization. By definition, general anesthetics reversibly produce all three of these therapeutic effects (3,4). General anesthetic drugs include inhaled gases as well as intravenous agents. Other classes of drugs may be used by anesthetists to achieve specific clinical goals during surgery (5). For example, anesthetists often use drugs that selectively inhibit neuromuscular transmission to reduce patient movement and facilitate surgery. Benzodiazepines may be used to provide anxiolysis and anterograde amnesia, and opioids provide analgesia (an action that is produced by only a few general anesthetics). However, among the many drugs used by anesthetists, general anesthetics are uniquely used to produce unconsciousness.

In contrast to early hypotheses that it represents a single global state change in the CNS, we now view general anesthesia as a combination of specific behavioral effects (2). Indeed, the ability of general anesthetics to produce distinct therapeutic endpoints is not equal. Explicit memory is the most sensitive target of inhaled general anesthetics; consciousness (assessed by appropriate response to spoken commands) is preserved in the absence of memory during exposure to low doses. Unconsciousness is produced by lower concentrations of most general anesthetics than those that ablate movement in response to noxious stimuli (6). Moreover, different drugs display distinct relative potencies in their ability to produce different components of general anesthesia. Thus, the ratio of the drug concentration producing immobility during incision to that producing unconsciousness is 1.5 for nitrous oxide, about 3 for most of the inhaled halogenated ethers, and over 4 for the intravenous anesthetic propofol.

Distinguishing among the clinical goals of general anesthesia is not simply of academic interest. An immobile patient is desired by surgeons to improve exposure and precision, whereas patients primarily wish for oblivion and amnesia during surgery, creating the potential for divergent goals. Cases of unintended and undesired consciousness occur in about 1 per 750 general anesthetics, due largely to the use of selective neuromuscular blockade together with inadequate general anesthetic doses (7). Thus, there is interest in identifying and monitoring neurophysiological correlates of consciousness during general anesthesia, in order to titrate dosing precisely to patient needs.

Recent molecular and transgenic animal studies have identified a number of targets where general anesthetics act to produce their effects, revealing that different types of general anesthetics act through distinct mechanisms, and perhaps even different neural circuits. This review focuses on the general anesthetic drugs and their molecular targets, specifically those linked to consciousness1.

Distinct Clinical Groups of General Anesthetics

We and others have classified the general anesthetics into three groups based on their relative potencies for different clinical endpoints and their impact on EEG. This clinical classification of general anesthetics has been shown to correlate remarkably well with studies identifying molecular targets of these drugs, particularly those targets associated with loss of consciousness (or its correlate in animals) (8,9). Group 1 consists of etomidate, propofol, and barbiturates, intravenous drugs that are much more potent at producing unconsciousness than immobilization. For propofol, which has been studied more than other Group 1 agents, hypnosis is achieved at plasma concentrations around 3 µg/ml, whereas immobility during skin incision requires four-fold higher concentrations (10). These drugs shift cortical EEG toward lower frequencies, enabling reproducible correlation with anesthetic depth (11). Group 2 includes the gaseous anesthetics nitrous oxide (N2O), xenon (Xe) and cyclopropane, along with ketamine, an intravenous agent. Clinically, these drugs produce significant analgesia, while their potency as hypnotics and immobilizers are relatively weak. In fact, no analgesia is demonstrable using group 1 and group 3 general anesthetics at concentrations below those producing unconsciousness. Cardiovascular stability and a high frequency of reported dreamlike experiences are also features associated with Group 2 (12). The ratio of doses (alveolar concentrations) producing immobility versus unconsciousness for N2O is only 1.5, while that for xenon is 2 (13). N2O and ketamine may increase cortical EEG frequencies, and EEG-based anesthetic depth monitoring is not reliable for detecting the effects of N2O and ketamine (14,15). There is conflicting data on whether EEG-based monitoring is useful for xenon (16,17). Group 3 consists of the volatile halogenated anesthetics: halothane, enflurane, isoflurane, sevoflurane, and desflurane. These drugs induce amnesia, hypnosis and immobility in a predictable manner (4,18). The ratio of doses producing immobility to those producing unconsciousness for Group 3 drugs is between that for propofol and N2O, ranging from 2 to 3 (6). Group 3 drugs produce amnesia at doses (partial pressures) lower than those that produce unconsciousness. Volatile anesthetics reduce the spectral edge frequency of cortical EEG and anesthetic depth monitors produce reliable correlations with the level of consciousness.

Etomidate, Propofol, and Barbiturates (Group 1)

Loss of righting reflexes (LORR) and immobility produced by etomidate, propofol, and barbiturates are mediated by a subset of γ-aminobutyric acid type A (GABAA) receptors. The GABAA receptors are neurotransmitter-gated chloride channels that are members of the ligand-gated ion channel superfamily that also contains nicotinic acetylcholine receptors, glycine receptors, and serotonin type 3 receptors. GABAA receptors are located both postsynaptically and extrasynaptically on neurons and when activated, they reduce neuronal excitation. Genetic techniques have identified 18 different GABAA receptor subunits: 6α’s, 3β’s, 3γ’s, δ, ε, π, and 3ρ’s (19). Typical postsynaptic GABAA receptors contain α, β, and γ subunits, while extrasynaptic channels lack γ subunits and often contain δ. Group 1 anesthetics enhance GABA-mediated channel activation and prolong post-synaptic inhibitory currents (IPSCs), suppressing neuronal excitability.

Evidence supporting a major role for GABAA receptors in unconsciousness caused by Group 1 drugs is plentiful. Etomidate has a chiral carbon and R(+)-etomidate is 20-fold more potent at inducing LORR than S(−)-etomidate (20). Stereospecificity of the same degree is observed in molecular studies of GABAA receptor modulation by etomidate. Critical studies linking specific types of GABAA receptors to anesthetic-induced unconsciousness evolved from identification of a role for the β subunit in sensitivity to etomidate and propofol. GABAA receptors containing β2 and β3 subunits are sensitive to etomidate and propofol, but not those containing β1 subunits (21). Researchers identified amino acids on β2 and β3 that, when mutated to their homologs on β1, reduced receptor modulation by etomidate and propofol (22). Mutations were introduced into the β2 and β3 genes of mice (knock-in transgenic animals). To induce LORR in mice containing the β3(N265M) mutation requires at least four-fold higher propofol or etomidate doses than those that produce LORR in wild-type litter-mates (23,24). The β3(N265M) transgenic mice are also resistant to LORR produced by pentobarbital (25). Intriguingly, mice containing the β2(N265S) mutation show normal etomidate and propofol sensitivity for LORR, but they are less sedated than wild-type mice at low drug doses (26). Thus, Group 1 anesthetics induce LORR, the most commonly used correlate of unconsciousness in animals, through GABAA receptors containing β3 subunits, whereas sedation seems linked to GABAA receptors containing β2 subunits.

Other studies have suggested that propofol-induced unconsciousness may be caused by indirect activation of cannabinoid receptors (27). Propofol was found to inhibit fatty acid amide hydrolase, an enzyme that degrades the endogenous cannabinoid receptor agonist, anandamide. This mechanism may also contribute to the unusual anti-emetic properties of propofol.

Nitrous oxide, Xenon, Cyclopropane and Ketamine (Group 2)

At clinical concentrations, the Group 2 drugs N2O, xenon, cyclopropane, and ketamine have little or no effect on GABAA receptors. Instead, these anesthetics potently inhibit N-methyl-D-aspartate (NMDA) receptors (2831), which are excitatory cation channels activated by the amino acid glutamate. Glutamate receptors are the major excitatory neurotransmitter-gated ion channels in mammalian brain, and are tetramers formed from a group of seven homologous subunits: NR1, NR2 (A through D), and NR3 (A and B). In the presence of N2O, Xe, cyclopropane or ketamine, NMDA receptor-mediated excitatory post-synaptic currents (EPSCs) are markedly inhibited, and presumably reduced excitatory signals in critical neuronal circuits causes unconsciousness.

Both pharmacological and molecular evidence supports a role for NMDA receptors in mediating unconsciousness produced by Group 2 drugs. Ketamine possesses a chiral carbon, and S-ketamine has been shown to be 4 times more potent than R-ketamine in human volunteers. In hippocampal neurons, S-ketamine also inhibits NMDA receptors more potently than R-ketamine (30), reflecting the relative stereoselectivity of in vivo actions. Transgenic mice lacking the NMDA receptor ε1 subunit (homologous to the human NR2A subunit) show resistance to LORR produced by ketamine (32,33) and by N2O (34). In the nematode Caenorhabditis Elegans, a null mutation of the NMDA-type receptor gene nmr-1 produces resistance to the effects of N2O (35), which are distinct from those produced by halogenated anesthetics. Furthermore, a mutation that confers resistance to halogenated anesthetics did not produce resistance to N2O (and vice versa). These behavioral observations parallel those in humans and mice, while the genetic experiments demonstrate that N2O and halogenated anesthetics have distinct molecular targets in C. Elegans. The behavioral effects of xenon in wild-type C. Elegans are similar to those produced by N2O, and are attenuated by knock-out of a non-NMDA glutamate receptor, glr-1 (36). This gene encodes a homolog of the human AMPA receptor. Xe and N2O inhibit both human NMDA and AMPA receptors (37).

Group 2 anesthetics also inhibit α4β2 neuronal nicotinic acetylcholine receptors (31,38), which modulate synaptic release of neurotransmitters. Ketamine stereospecificity has also been demonstrated in these receptors (39). Neuronal nicotinic receptors have not been linked to LORR, but they may be important mediators of anesthetic-induced amnesia (40). Group 2 general anesthetics also affect members of the two-pore (2P) domain potassium channel family (41). TREK-1 is an anesthetic-sensitive “background leak” K+ channel that regulates the resting membrane potential of neurons. TREK-1 channels are also activated by volatile anesthetics (42) and TREK-1 knockout mice have been created (43). The knock-out of TREK-1 reduces sensitivity to volatile anesthetic-induced LORR (see below), but testing against Group 2 drugs has not been reported.

Halogenated Volatile Anesthetics (Group 3)

Group 3 general anesthetics, comprising the halogenated ethers and alkanes, lack significant selectivity for general anesthetic target molecules (18). Group 3 drugs positively modulate GABAA receptors and closely related inhibitory glycine receptors, while also activating 2P potassium channels and inhibiting a variety of excitatory cation channels. There is also evidence that Group 3 drugs act at pre-synaptic targets.

Volatile general anesthetics enhance the function of inhibitory GABAA and glycine receptors. Studies of GABAA receptors have identified amino acid residues located in the transmembrane domains of both α and β subunits that determine anesthetic sensitivity. Pharmacologic and genetic studies, however, suggest that volatile anesthetics produce unconsciousness via different GABAA receptor subunits than those targeted by the Group 1 drugs. Transgenic mice that lack the GABAA receptor β3 subunit show no change in their volatile anesthetic requirement for LORR (44). Transgenic mice bearing the point mutation β3(N265M) also show little change in volatile anesthetic concentrations associated with LORR (23), whereas this β3 mutation significantly reduces in vitro receptor modulation by Group 3 drugs. Surprisingly, β3(N265M) mice also show a small reduction in sensitivity for cyclopropane, which has minimal effects on GABAA receptors in vitro. Another well-studied mutation on GABAA receptors is α1 serine to histidine at position 270, which eliminates in vitro receptor enhancement by isoflurane and desflurane, but not halothane (45). Knock-in mice with the α1(S270H) mutation require moderately increased concentrations of isoflurane and enflurane to induce LORR (46). These transgenic animals showed no change in sensitivity to halothane, and volatile anesthetic concentrations producing immobility in α1(S270H) mice matched those in wild-type mice. Thus, α1 subunit-containing GABAA receptors contribute to Group 3 LORR, but not immobility.

Many two-pore domain K+ channels, including TREK-1, TREK-2, TASK-1, TASK-2, TASK-3 and TRESK are activated by Group 3 general anesthetics (42,47). TREK-1 and TASK-3 knockout mice required increased amounts of volatile anesthetic to produce both LORR and immobility (43,48). However, other volatile anesthetic-sensitive K+ channels such as TASK-2 appear to play little or no role in anesthesia (49), though they may produce some side-effects.

Like N2O, Xe and cyclopropane, the volatile anesthetics also inhibit excitatory glutamate receptors in the CNS (50). However, NMDA receptor ε1 subunit knockout mice that exhibit resistance to ketamine and N2O show no resistance to sevoflurane (34). Similarly, kainate receptor GluR6 subunit knockout mice exhibit minimal changes in volatile anesthetic sensitivity for immobility or fear conditioning (51). The latter requires both consciousness and learning. Other glutamate receptor subunits may mediate actions of volatile anesthetics.

A wide range of other ion channels are sensitive to Group 3 general anesthetics, including neuronal nicotinic acetylcholine receptors (52), serotonin type 3 receptors (53), Na+ channels (54), mitochondrial ATP-sensitive K+ channels (55), and cyclic nucleotide-gated HCN channels that mediate neuronal pacemaker currents (56). Some of these receptors may play important roles in mediating unconsciousness, but testing in genetically altered animals has not yet occurred.

Dexmedetomidine

Dexmedetomidine is an unusual intravenous drug that produces some behavioral effects that are characteristic of general anesthetics and illustrative of how distinct behavioral effects of sedative/hypnotic drugs may be mediated by quite specific neural circuits. The molecular targets of dexmedetomidine and the neural networks involved in its sedative action have been characterized in detail. The molecular targets for dexmedetomidine are central α2-adrenergic receptors (α2-AdRs) (57). Activation of spinal α2-AdRs is linked to dexmedetomidine’s anti-nociceptive activity, while α2-AdRs in sleep circuits of the brainstem, specifically the locus ceruleus (LC), mediate sedation that mimics NREM sleep. Selective ablation of the LC or injection of α2-AdR inhibitor into the LC prevents dexmedetomidine-induced sedation (58).

In addition, the sedative actions of Group 1 general anesthetics are apparently mediated by GABAA receptors in another sleep circuit nucleus of the brainstem, the tubo-mammilary nucleus (TMN) (59). A critical link between the LC and the TMN is the ventro-lateral pre-optic nucleus (VLPO), which is disinhibited when dexmedetomidine activates α2-AdRs in the TMN. The VLPO in turn inhibits the TMN via GABA release, causing effects similar to those caused by selective injection of Group 1 anesthetics or GABA agonists in this nucleus. Despite this link, dexmedetomidine is another drug that does not cause reliable slowing of cortical EEG frequencies (60). Indeed, it does not reliably produce unconsciousness, and is therefore proving useful for “awake craniotomy” cases that require patients to communicate with surgeons during cortical mapping (61).

Anesthetic Targets and Models of Drug-Induced Unconsciousness

Consciousness is a complex mechanism for filtering and processing both new primary information and memory into high-fidelity subjective constructs (qualia). In the context of this functional model of the conscious mind, impaired consciousness represents reduction or elimination of subjective construct fidelity, which can be caused by 1) impaired primary sensation; 2) impaired filtering of information or 3) impaired processing. There is little support for the idea that general anesthetics impair primary sensation (detection, reception). Group 2 drugs such as ketamine and nitrous oxide appear to act by impairing signal filtration, perhaps altering the amounts or intensities of sensory inputs, or by allowing leakage of unconscious signals into conscious processing. This lack of filtering “jams” or overwhelms the mind with signals, leading to a “dissociative” state, frequently associated with reports of vivid dreamlike experiences. In parallel, cortical activity is maintained or increased by nitrous oxide and ketamine, suggesting enhanced, perhaps overloaded neural activity. Interestingly, ketamine and nitrous oxide have minimal effects on important “unconscious” processes such as breathing and blood-pressure regulation, suggesting that their actions are somewhat selective for filtering functions, which probably occur in sub-cortical circuits. Thus, information filtering seems to be particularly sensitive to drugs that inhibit excitatory glutamate and nicotinic acetylcholine receptors in the CNS.

In contrast, Group 1 and Group 3 drugs that act by enhancing GABAA receptor activity appear to impair both unconscious and conscious processing, including associative cortex and synchronous integration among multiple cortical areas. Cortical EEG activity shifts toward lower frequencies in the presence of these drugs, and large doses can lead to the total loss of coordinated neural activity (iso-electric EEG). Unconscious processes are suppressed by these general anesthetics less than the conscious mind, perhaps because they are less dependent on coordinated integration among multiple cortical areas.

Conclusions

General anesthetics are the sole class of drugs used by physicians for inducing unconsciousness. General anesthetics have traditionally been considered to be non-specific drugs with widespread effects on the CNS. As a result, it has long been thought that these drugs can teach us little about the nature of consciousness or the mechanisms through which it can be inhibited. However, molecular pharmacology and transgenic animal studies are revealing that some general anesthetics are in fact quite selective for important CNS targets and structures that are critical for modulating the processes associated with consciousness. As we discover more about the plethora of molecules involved in neuronal activity and the anatomy of the circuitry in which they function, studies of general anesthetic mechanisms will likely reveal more important insights into how consciousness is modulated. At the same time, this information will be critical for designing improved anesthetic agents with more selectivity against consciousness.

Acknowledgments

Support for this work was provided by NIH grants to SAF (GM66724 and GM58448) and the MGH Dept. of Anesthesia & Critical Care.

Footnotes

1Research linking general anesthetic targets to behavior has been carried out in laboratory animals, such as transgenic mice. Using animals requires measuring correlates of human behavior. For example, the most commonly used surrogate for unconsciousness in animals is loss of righting reflexes (LORR), a test of response to a non-noxious postural change. Whether these experimental observations in mice are relevant to the nuances of human consciousness is not known.

Contributor Information

Stuart A. Forman, Dept. of Anaesthesia, Harvard Medical School, Boston, MA; Dept. of Anesthesia & Critical Care, Massachusetts General Hospital, Boston, MA.

Victor A. Chin, Dept. of Anesthesia & Critical Care, Massachusetts General Hospital, Boston, MA.

Citations

1. Nuland SB. Doctors : the biography of medicine. 1st ed. New York: Knopf; 1988.
2. Kissin I. A concept for assessing interactions of general anesthetics. Anesth Analg. 1997;85:204–210. [PubMed]
3. Grasshoff C, Rudolph U, Antkowiak B. Molecular and systemic mechanisms of general anaesthesia: the 'multi-site and multiple mechanisms' concept. Current Opinion in Anaesthesiology. 2005;18:386–391. [PubMed]
4. Mashour GA, Forman SA, Campagna JA. Mechanisms of general anesthesia: from molecules to mind. Best Pract Res Clin Anaesthesiol. 2005;19:349–364. [PubMed]
5. Woodbridge PD. Changing concepts concerning depth of anesthesia. Anesthesiology. 1957;18:536–550. [PubMed]
6. Eger EI., 2nd Age, minimum alveolar anesthetic concentration, and minimum alveolar anesthetic concentration-awake. Anesthesia & Analgesia. 2001;93:947–953. [PubMed]
7. Forman SA. Awareness During General Anesthesia: Concepts and Controversies. Seminars in Anesthesia, Perioperative Medicine and Pain. 2006;25:211–218.
8. Grasshoff C, Drexler B, Rudolph U, Antkowiak B. Anaesthetic drugs: linking molecular actions to clinical effects. Curr Pharm Des. 2006;12:3665–3679. [PubMed]
9. Solt K, Forman SA. Correlating the clinical actions and molecular mechanisms of general anesthetics. Curr Opin Anaesthesiol. 2007;20:300–306. [PubMed]
10. Smith C, McEwan AI, Jhaveri R, et al. The interaction of fentanyl on the Cp50 of propofol for loss of consciousness and skin incision. Anesthesiology. 1994;81:820–828. [PubMed]
11. Kuizenga K, Wierda JM, Kalkman CJ. Biphasic EEG changes in relation to loss of consciousness during induction with thiopental, propofol, etomidate, midazolam or sevoflurane. Br J Anaesth. 2001;86:354–360. [PubMed]
12. Lynch C, 3rd, Baum J, Tenbrinck R. Xenon anesthesia. Anesthesiology. 2000;92:865–868. [PubMed]
13. Goto T, Nakata Y, Ishiguro Y, et al. Minimum alveolar concentration-awake of Xenon alone and in combination with isoflurane or sevoflurane. Anesthesiology. 2000;93:1188–1193. [PubMed]
14. Anderson RE, Jakobsson JG. Entropy of EEG during anaesthetic induction: a comparative study with propofol or nitrous oxide as sole agent. Br J Anaesth. 2004;92:167–170. [PubMed]
15. Rampil IJ, Kim JS, Lenhardt R, et al. Bispectral EEG index during nitrous oxide administration. Anesthesiology. 1998;89:671–677. [PubMed]
16. Laitio RM, Kaskinoro K, Sarkela MO, et al. Bispectral index, entropy, and quantitative electroencephalogram during single-agent xenon anesthesia. Anesthesiology. 2008;108:63–70. [PubMed]
17. Goto T, Nakata Y, Saito H, et al. Bispectral analysis of the electroencephalogram does not predict responsiveness to verbal command in patients emerging from xenon anaesthesia. Br J Anaesth. 2000;85:359–363. [PubMed]
18. Campagna JA, Miller KW, Forman SA. Mechanisms of Actions of Inhaled Anesthetics. New England Journal of Medicine. 2003;348:2110–2124. [PubMed]
19. Whiting PJ. The GABA-A receptor gene family: new targets for therapeutic intervention. Neurochemistry International. 1999;34:387–390. [PubMed]
20. Husain SS, Ziebell MR, Ruesch D, et al. 2-(3-Methyl-3H-diaziren-3-yl)ethyl 1-(1-phenylethyl)-1H-imidazole-5-carboxylate: a derivative of the stereoselective general anesthetic etomidate for photolabeling ligand-gated ion channels. Journal of Medicinal Chemistry. 2003;46:1257–1265. [PubMed]
21. Hill-Venning C, Belelli D, Peters JA, Lambert JJ. Subunit-dependent interaction of the general anaesthetic etomidate with the gamma-aminobutyric acid type A receptor. British Journal of Pharmacology. 1997;120:749–756. [PubMed]
22. Belelli D, Lambert JJ, Peters JA, et al. The interaction of the general anesthetic etomidate with the gamma-aminobutyric acid type A receptor is influenced by a single amino acid. Proceedings of the National Academy of Sciences of the United States of America. 1997;94:11031–11036. [PubMed]
23. Jurd R, Arras M, Lambert S, et al. General anesthetic actions in vivo strongly attenuated by a point mutation in the GABA(A) receptor beta3 subunit. FASEB Journal. 2003;17:250–252. [PubMed]
24. Liao M, Sonner JM, Husain SS, et al. R (+) etomidate and the photoactivable R (+) azietomidate have comparable anesthetic activity in wild-type mice and comparably decreased activity in mice with a N265M point mutation in the gamma-aminobutyric acid receptor beta3 subunit. Anesthesia & Analgesia. 2005;101:131–135. [PubMed]
25. Zeller A, Arras M, Jurd R, Rudolph U. Identification of a molecular target mediating the general anesthetic actions of pentobarbital. Mol Pharmacol. 2007;71:852–859. [PubMed]
26. Reynolds DS, Rosahl TW, Cirone J, et al. Sedation and anesthesia mediated by distinct GABA(A) receptor isoforms. Journal of Neuroscience. 2003;23:8608–8617. [PubMed]
27. Patel S, Wohlfeil ER, Rademacher DJ, et al. The general anesthetic propofol increases brain N-arachidonylethanolamine (anandamide) content and inhibits fatty acid amide hydrolase. Br J Pharmacol. 2003;139:1005–1013. [PubMed]
28. Jevtovic-Todorovic V, Todorovic SM, Mennerick S, et al. Nitrous oxide (laughing gas) is an NMDA antagonist, neuroprotectant and neurotoxin. Nat Med. 1998;4:460–463. [PubMed]
29. Franks NP, Dickinson R, de Sousa SL, et al. How does xenon produce anaesthesia? Nature. 1998;396:324. [PubMed]
30. Zeilhofer HU, Swandulla D, Geisslinger G, Brune K. Differential effects of ketamine enantiomers on NMDA receptor currents in cultured neurons. Eur J Pharmacol. 1992;213:155–158. [PubMed]
31. Raines DE, Claycomb RJ, Scheller M, Forman SA. Nonhalogenated alkane anesthetics fail to potentiate agonist actions on two ligand-gated ion channels. Anesthesiology. 2001;95:470–477. [PubMed]
32. Petrenko AB, Yamakura T, Fujiwara N, et al. Reduced sensitivity to ketamine and pentobarbital in mice lacking the N-methyl-D-aspartate receptor GluRepsilon1 subunit. Anesth Analg. 2004;99:1136–1140. [PubMed]
33. Sato Y, Kobayashi E, Hakamata Y, et al. Chronopharmacological studies of ketamine in normal and NMDA epsilon1 receptor knockout mice. Br J Anaesth. 2004;92:859–864. [PubMed]
34. Sato Y, Kobayashi E, Murayama T, et al. Effect of N-methyl-D-aspartate receptor epsilon1 subunit gene disruption of the action of general anesthetic drugs in mice. Anesthesiology. 2005;102:557–561. [PubMed]
35. Nagele P, Metz LB, Crowder CM. Nitrous oxide (N(2)O) requires the N-methyl-D-aspartate receptor for its action in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2004;101:8791–8796. [PubMed]
36. Nagele P, Metz LB, Crowder CM. Xenon acts by inhibition of non-N-methyl-D-aspartate receptor-mediated glutamatergic neurotransmission in Caenorhabditis elegans. Anesthesiology. 2005;103:508–513. [PubMed]
37. Yamakura T, Harris RA. Effects of gaseous anesthetics nitrous oxide and xenon on ligand-gated ion channels. Comparison with isoflurane and ethanol. Anesthesiology. 2000;93:1095–1101. [PubMed]
38. Coates KM, Flood P. Ketamine and its preservative, benzethonium chloride, both inhibit human recombinant alpha7 and alpha4beta2 neuronal nicotinic acetylcholine receptors in Xenopus oocytes. Br J Pharmacol. 2001;134:871–879. [PubMed]
39. Friederich P, Dybek A, Urban BW. Stereospecific interaction of ketamine with nicotinic acetylcholine receptors in human sympathetic ganglion-like SH-SY5Y cells. Anesthesiology. 2000;93:818–824. [PubMed]
40. Raines DE, Claycomb RJ, Forman SA. Nonhalogenated anesthetic alkanes and perhalogenated nonimmobilizing alkanes inhibit alpha(4)beta(2) neuronal nicotinic acetylcholine receptors. Anesth Analg. 2002;95:573–577. [PubMed]
41. Gruss M, Bushell TJ, Bright DP, et al. Two-pore-domain K+ channels are a novel target for the anesthetic gases xenon, nitrous oxide, and cyclopropane. Mol Pharmacol. 2004;65:443–452. [PubMed]
42. Franks NP, Honore E. The TREK K2P channels and their role in general anaesthesia and neuroprotection. Trends Pharmacol Sci. 2004;25:601–608. [PubMed]
43. Heurteaux C, Guy N, Laigle C, et al. TREK-1, a K+ channel involved in neuroprotection and general anesthesia. Embo J. 2004;23:2684–2695. [PubMed]
44. Quinlan JJ, Homanics GE, Firestone LL. Anesthesia sensitivity in mice that lack the beta3 subunit of the gamma-aminobutyric acid type A receptor. Anesthesiology. 1998;88:775–780. [PubMed]
45. Borghese CM, Werner DF, Topf N, et al. An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice. J Pharmacol Exp Ther. 2006;319:208–218. [PubMed]
46. Sonner JM, Werner DF, Elsen FP, et al. Effect of isoflurane and other potent inhaled anesthetics on minimum alveolar concentration, learning, and the righting reflex in mice engineered to express alpha1 gamma-aminobutyric acid type A receptors unresponsive to isoflurane. Anesthesiology. 2007;106:107–113. [PubMed]
47. Patel AJ, Honore E, Lesage F, et al. Inhalational anesthetics activate two-pore-domain background K+ channels. Nature Neuroscience. 1999;2:422–426. [PubMed]
48. Linden AM, Sandu C, Aller MI, et al. TASK-3 knockout mice exhibit exaggerated nocturnal activity, impairments in cognitive functions, and reduced sensitivity to inhalation anesthetics. J Pharmacol Exp Ther. 2007;323:924–934. [PubMed]
49. Gerstin KM, Gong DH, Abdallah M, et al. Mutation of KCNK5 or Kir3.2 potassium channels in mice does not change minimum alveolar anesthetic concentration. Anesth Analg. 2003;96:1345–1349. [PubMed]
50. Dildy-Mayfield JE, Eger EI, 2nd, Harris RA. Anesthetics produce subunit-selective actions on glutamate receptors. J. Pharm. Exp. Ther. 1996;276:1058–1065. [PubMed]
51. Sonner JM, Vissel B, Royle G, et al. The effect of three inhaled anesthetics in mice harboring mutations in the GluR6 (kainate) receptor gene. Anesth Analg. 2005;101:143–148. [PubMed]
52. Flood P, Role LW. Neuronal nicotinic acetylcholine receptor modulation by general anesthetics. Toxicol. Lett. 1998;100–101:149–153. [PubMed]
53. Stevens RJ, Rusch D, Davies PA, Raines DE. Molecular properties important for inhaled anesthetic action on human 5-HT3A receptors. Anesth Analg. 2005;100:1696–1703. [PubMed]
54. Roch A, Shlyonsky V, Goolaerts A, et al. Halothane directly modifies Na+ and K+ channel activities in cultured human alveolar epithelial cells. Mol Pharmacol. 2006;69:1755–1762. [PubMed]
55. Turner LA, Fujimoto K, Suzuki A, et al. The interaction of isoflurane and protein kinase C-activators on sarcolemmal KATP channels. Anesth Analg. 2005;100:1680–1686. [PubMed]
56. Chen X, Sirois JE, Lei Q, et al. HCN subunit-specific and cAMP-modulated effects of anesthetics on neuronal pacemaker currents. J Neurosci. 2005;25:5803–5814. [PubMed]
57. Sanders RD, Maze M. Alpha2-adrenoceptor agonists. Curr Opin Investig Drugs. 2007;8:25–33. [PubMed]
58. Nelson LE, Lu J, Guo T, et al. The alpha2-adrenoceptor agonist dexmedetomidine converges on an endogenous sleep-promoting pathway to exert its sedative effects. Anesthesiology. 2003;98:428–436. [PubMed]
59. Nelson LE, Guo TZ, Lu J, et al. The sedative component of anesthesia is mediated by GABA(A) receptors in an endogenous sleep pathway. Nat Neurosci. 2002;5:979–984. [PubMed]
60. Bol CJ, Vogelaar JP, Mandema JW. Anesthetic profile of dexmedetomidine identified by stimulus-response and continuous measurements in rats. J Pharmacol Exp Ther. 1999;291:153–160. [PubMed]
61. Souter MJ, Rozet I, Ojemann JG, et al. Dexmedetomidine sedation during awake craniotomy for seizure resection: effects on electrocorticography. J Neurosurg Anesthesiol. 2007;19:38–44. [PubMed]