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Logo of jbcThe Journal of Biological Chemistry
 
J Biol Chem. 2013 May 31; 288(22): 16139–16144.
Published online 2013 April 16. doi:  10.1074/jbc.M113.456780
PMCID: PMC3668769

Ataxia Telangiectasia-mutated- and Rad3-related Protein Regulates the DNA Damage-induced G2/M Checkpoint through the Aurora A Cofactor Bora Protein*An external file that holds a picture, illustration, etc.
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Bo Qin, Bowen Gao, Jia Yu,§ Jian Yuan,¶,1 and Zhenkun Lou‡,2

Abstract

Polo-like kinase1 (Plk1) activation is inhibited in response to DNA damage, and this inhibition contributes to the activation of the G2/M checkpoint, although the molecular mechanism by which Plk1 is inhibited is not clear. Here we report that the DNA damage signaling pathway inhibits Plk1 activity through Bora. Following UV irradiation, ataxia telangiectasia-mutated- and Rad3-related protein phosphorylates Bora at Thr-501. The phosphorylated Thr-501 is subsequently recognized by the E3 ubiquitin ligase SCF-β-TRCP, which targets Bora for degradation. The degradation of Bora compromises Plk1 activation and contributes to DNA damage-induced G2 arrest. These findings shed new light on Plk1 regulation by the DNA damage response pathway.

Keywords: Cell Biology, Cell Cycle, Checkpoint Control, DNA Damage Response, Protein Degradation

Introduction

Plk1 is a multi-functional protein controlling many processes in the cell cycle, including centrosome maturation, bipolar spindle formation, sister chromatid cohesion, activation of anaphase-promoting complex/cyclosome, control of cleavage furrow formation, and mitotic entry (1, 2). Plk1 promotes mitotic entry by inhibiting Wee1 (3), activating Cdc25 (4, 5) and inducing cyclin B nuclear import (6), which, in turn, activates cyclin B/Cdk1. Plk1 itself is activated by phosphorylation in its activation loop (Thr-210 in mammals) (4, 79). The mechanism responsible for Thr-210 phosphorylation has been elusive until two recent reports demonstrated that Bora cooperates with Aurora A to promote Plk1 phosphorylation at Thr-210 and Plk1 activation (10, 11).

Plk1 has also been shown to be a critical target of the DNA damage response pathway. In response to DNA damage, Plk1 activity is inhibited in an ataxia telangiectasia-mutated/ATR-dependent3 manner, and overexpression of constitutively active Plk1 overrides the DNA damage-induced G2/M checkpoint (1214). These findings suggest that inhibition of Plk1 is an important mechanism for activation of the G2/M checkpoint. Although Plk1 was speculated to be a direct target of ataxia telangiectasia-mutated /ATR-mediated signaling, the molecular mechanism of Plk1 inhibition remains unclear. Here we show that DNA damage leads to the phosphorylation of Bora by ATR, which is subsequently targeted for proteasome-mediated degradation. The resulting reduction in Bora protein levels causes inhibition of Plk1 and G2 arrest.

EXPERIMENTAL PROCEDURES

Plasmids

S/FLAG/streptavidin-binding peptide-tagged Bora was cloned into pIRES2-EGFP (Clontech). The Bora T501A mutant and Bora shRNA-resistant constructs were generated with a PCR-based mutagenesis kit (Stratagene).

Antibody and Chemicals

Bora antibody was raised by immunizing rabbit with N-terminal (1–210) Bora-GST fusion protein. pT501 Bora antibody was raised by using the phosphopeptide CMDSGYNpTQN.

Other antibodies used were anti-Bora (Epitomics), anti-FLAG (M2) (Sigma), β-actin antibody (Sigma), phospho-SQ/TQ antibody (Cell Signaling Technology, Inc.), β-TRCP antibody (Invitrogen), Chk1 antibody (Santa Cruz Biotechnology, Inc.), Chk1-pS317 antibody (Cell Signaling Technology, Inc.), ATR antibody (Genetex), Plk1-pT210 antibody (BD Biosciences), Plk1 antibody (Invitrogen), and phospho-H3 antibody (Abcam). The chemicals used were MG132 (Sigma) and ON01910 (a gift from Dr. Zheng Fu, Virginia Commonwealth University).

Quantitative Real-time PCR

mRNA was extracted with a PARIS kit (Applied Biosystems). Quantitative real-time PCR was carried out with a one-step Brilliant II SYBR Green QRT-PCR Master Mix kit (Agilent Technologies). Primers of human Bora and GAPDH were purchased from Qiagen. Bora mRNA expression levels were calculated on the basis of the 2−ΔCt value normalized to GAPDH.

Cell Culture

293T, H1299, A549, and U2OS were maintained in RPMI 1640 medium with 10% fetal bovine serum at 37 °C in 5% CO2. For the cell cycle, cells were fixed with cold 70% (v/v) ethanol, stained with propidium iodide, and then run on the fluorescence activated cell sorter machine.

Immunoprecipitation and Western Blot Analysis

Cells were lysed with NETN buffer (20 mm Tris-HCl (pH 8.0), 100 mm NaCl, 1 mm EDTA, and 0.5% Nonidet P-40) containing 50 mm glycerophosphate, 10 mm NaF, and protease inhibitor mixture on ice for 20 min. The cell lysates were obtained by centrifugation and then incubated with anti-FLAG beads (M2, Sigma) or protein A beads bound to anti-Bora antibody for 1 h at 4 °C. The immunocomplexes were then washed with NETN buffer three times and separated by SDS-PAGE. Immunoblotting was performed following standard procedures. Band intensity was quantified by ImageJ analysis (National Institutes of Health).

RNA Interference

Bora shRNAs (NM_024808.2), β-TRCP shRNA (NM_003939.2), and ATR shRNA (NM_001184) were purchased from Sigma. The Bora shRNA target sequence was as follows: GCTTAAGAGTTCCTCGCATAT (targeted to the 3′ UTR of Bora mRNA). The β-TRCP shRNA target sequence was as follows: GCGTTTCAATAATGGCATGAT, GCGTTGTATTCGATTTGATAA, CCATTAAAGTTGCGGTATTTA, GCACATAAACTCGTATCTTAA, GCTGAACTTGTGTGCAAGGAA. The ATR shRNA target sequence was as follows: GCCGCTAATCTTCTAACATTA, GCCAAAGTATTTCTAGCCTAT, CTGTGGTTGTATCTGTTCAAT. Lentiviruses of Bora, β-TRCP, and ATR shRNAs were made according to the protocol of the manufacturer.

Immunofluorescence

Bora knockdown U2OS cells were plated on glass coverslips and transfected with the indicated constructs. 48 h after transfection, cells were left untreated or treated with UV radiation (20 J/m2). After the indicated time, cells were fixed in 3% paraformaldehyde for 10 min at room temperature and then stained with phospho-H3 antibody using the standard protocol.

Statistics

Experiments with three replicates were carried out, and statistical analyses were performed by two-tailed Student's t test. Values of p < 0.05 were considered significant.

RESULTS AND DISCUSSION

Degradation of Bora following DNA Damage

During our investigation of Bora, we unexpectedly found that Bora protein levels rapidly decreased after UV treatment (Fig. 1A). The decrease in Bora levels was both time- and dose-dependent (Fig. 1, A and B). To examine whether the decrease in Bora protein levels was a general response to genotoxic stresses, we used other DNA damage inducers. As shown in Fig. 1C, camptothecin (CPT) induced down-regulation of Bora protein. However, we did not observe a significance change in Bora level following ionizing radiation (IR), suggesting that the Bora level is regulated by replication stress. Our initial findings used epitope-tagged Bora (Fig. 1, A–C). We further confirmed these findings by examining the levels of endogenous Bora after DNA damage. As shown in Fig. 1D, endogenous Bora levels decreased with similar kinetics as those of recombinant Bora.

FIGURE 1.
Bora is degraded in a proteasome-dependent manner following DNA damage. A–C, 293T cells were transfected with a construct encoding FLAG-Bora. Cells were left untreated or treated with UV (20 J/m2), camptothecin (CPT, 1 μm), irradiation ...

We next examined the mechanism underlying DNA damage-induced Bora down-regulation. Because Bora levels change throughout the cell cycle (15, 16), it is possible that the apparent down-regulation of Bora is due to an indirect effect of cell cycle populations. However, the down-regulation of Bora occurred quickly (as early as 1 h) following DNA damage, whereas the overall cell cycle profile did not change significantly at this time (Fig. 1E). Therefore, it is unlikely that the change in Bora levels is due to a change in cell cycle populations. We also found that Bora mRNA levels did not change significantly following UV irradiation (Fig. 1F), suggesting that Bora down-regulation is regulated at the posttranscriptional level. Previous work has demonstrated that Bora is degraded by the ubiquitin-proteasome pathway and regulates mitotic exit (15, 16). It is possible that Bora protein levels are also regulated by the ubiquitin-proteasome pathway following DNA damage. To test this hypothesis, we pretreated cells with the proteasome inhibitor MG132. As shown in Fig. 1G, MG132 inhibited the UV-induced down-regulation of Bora. Furthermore, we observed increased Bora ubiquitination following UV irradiation (Fig. 1H). These results suggest that Bora is degraded through the proteasome-mediated pathway following DNA damage.

Phosphorylation of Bora at Thr-501 by ATR

To investigate how Bora is regulated by the DNA damage response pathway, we first examined whether Bora could be phosphorylated following DNA damage. As shown in supplemental Fig. S1A, Bora was phosphorylated at the SQ/TQ motifs, which are ataxia telangiectasia-mutated/ATR consensus phosphorylation motifs. Among the possible SQ/TQ motifs of Bora, the TQ motif at amino acid 501 of Bora is the only conserved one from human to Xenopus (supplemental Fig. S2). To confirm that this site is phosphorylated after DNA damage, we generated a phosphospecific antibody against Thr-501. As shown in Fig. 2A, WT Bora was phosphorylated at Thr-501 following DNA damage, whereas mutation at Thr-501 (T501A) abolished Bora phosphorylation. We also confirmed that phosphorylation of endogenous Bora at Thr-501 was increased after UV irradiation (Fig. 2B). Interestingly, Thr-501 is localized at the DS497GXXT501 motif, which acts as a degron for Bora (16). Both Ser-497 and Thr-501 have been shown to be phosphorylated by Plk1 during mitosis, and the phosphorylation of Ser-497/Thr-501 is important for the binding of β-TRCP to Bora and subsequent Bora degradation during mitotic exit (16). However, the phosphorylation of Thr-501 following DNA damage does not require Plk1 because the Plk1 inhibitor ON01910 (17) had no effect on Thr-501 phosphorylation (Fig. 2C). Instead, the phosphorylation of Thr-501 following DNA damage required PI3K-like kinases because the pan-PI3K-like kinase inhibitor caffeine (18) inhibited Thr-501 phosphorylation of both ectopically expressed and endogenous Bora (Fig. 2C and supplemental Fig. S1B). Because ATR is an upstream PI3K-like kinase activated by replication stress, we down-regulated ATR and found that down-regulation of ATR significantly inhibited endogenous Bora phosphorylation at Thr-501 (Fig. 2D). These results suggest that ATR phosphorylates Thr-501 of Bora following UV radiation.

FIGURE 2.
Bora is phosphorylated by ATR at Thr-501, which targets Bora to β-TRCP-mediated degradation. A, 293T cells transfected with constructs encoding FLAG-WT Bora or T501A Bora (T501A) were irradiated with UV light, and 4 h later, cells were lysed and ...

Phosphorylation of Bora at T501 is Required for β-TRCP Binding and Bora Degradation

Because phospho-Thr-501 is a β-TRCP binding site (16), we hypothesized that ATR mediated phosphorylation at Thr-501 resulted in β-TRCP binding and Bora degradation. Consistent with our hypothesis, mutating Thr-501 to Ala prevented DNA damage-induced ubiquitination and down-regulation of Bora (Fig. 2, E and F). The Bora T501A mutant was also more stable than WT Bora after DNA damage (Fig. 2G). These results suggest that Thr-501 is critical for Bora degradation following DNA damage. To confirm that phospho-Thr-501 is recognized by β-TRCP following DNA damage, we performed coimmunoprecipitation experiments. As shown in Fig. 2H, DNA damage induced the interaction between WT Bora and β-TRCP. However, the binding between the T501A mutant and β-TRCP could not be detected. To further confirm that β-TRCP is required for DNA damage-induced degradation of Bora, we used shRNA to knock down β-TRCP. As shown in Fig. 2I, the degradation of Bora was abolished in cells depleted of β-TRCP. These results suggest that DNA damage-induced phosphorylation of Bora at Thr-501 is required for β-TRCP binding and subsequent Bora degradation.

Phosphorylation of Bora at Thr-501 Is Important for G2/M Checkpoint Activation

Because Bora is important for Plk1 activation during mitotic entry (10, 11), we hypothesized that Plk1 inhibition after DNA damage is caused by decreased Bora levels. To test this hypothesis, we transfected cells with shRNA-resistant WT and T501A Bora, and depleted endogenous Bora with shRNA. Cells were then UV-irradiated. Consistent with previous reports (1214), the phosphorylation of Plk1 Thr-210 was decreased following DNA damage, and this correlated with the lower wild-type Bora protein level. We did not observe a significant decrease of Plk1 levels. This is different from a previous report (19) reporting Plk1 degradation induced by DNA damage. However, our results are consistent with several other reports (1214) (Fig. 3). These results suggest that phosphorylation and degradation of Bora is important for Plk1 inhibition following DNA damage.

FIGURE 3.
Degradation of Bora is required for G2/M checkpoint activation. U2OS cells were infected with lentiviruses encoding Bora shRNA. 48 h later, cells were transfected with shRNA-resistant WT or T501A Bora. Cells were left untreated or UV-irradiated (20 J/m ...

Plk1 activation is an important trigger for the mitotic entry, and impaired Plk1 activity decreases the population of mitotic cells, which contributes to the G2/M arrest. To explore the effect of Bora on G2/M checkpoint activation after DNA damage, we examined the G2/M checkpoint activation in cells overexpressing WT or T501A Bora. We found that DNA damage resulted in a significant decrease of the mitotic population in cells expressing WT Bora within 1 h of UV irradiation, suggesting that the G2/M checkpoint is intact in these cells. Conversely, there was no significant change in mitotic population in cells expressing T501A Bora. On the other hand, the mitotic population in both WT and T501A Bora-expressing cells decreased with IR treatment. This is consistent with the observation that Bora levels were not regulated by IR (Fig. 1C). These results suggest that Thr-501 phosphorylation of Bora is important for UV-induced but not IR-induced G2/M checkpoint activation (Fig. 3, B and C).

Bora degradation has been shown to be important to mitotic exit (15, 16). To exclude the possibility that the accumulation of mitotic cells in cells expressing mutant Bora is due to mitotic exit defect, we treated cells with UV radiation and then incubated cells in the presence of nocodazole. Consistent with Fig. 3, B and C, in cells expressing WT Bora, fewer cells entered mitosis after UV treatment. However, mitotic entry was less affected in cells expressing T501A Bora (Fig. 3D). These results suggest that UV-induced degradation of Bora is required for Plk1 inhibition and G2 arrest.

Cell cycle progression is under tight regulation to maintain the genomic integrity. The G2/M checkpoint stops cells from undergoing mitosis upon DNA damage until damaged DNA is repaired. One of the major targets of the G2/M checkpoint is Plk1, which is inactivated following DNA damage. However, how the DNA damage response pathway regulates Plk1 activity is not clear. Here we show that Bora is a direct target of ATR and that the phosphorylation of Bora by ATR results in Bora degradation, which is required for Plk1 inhibition and the activation of the G2/M checkpoint. We propose a working model in Fig. 4. During the normal cell cycle, Bora is up-regulated in G2 phase and binds to the Plk1 Polo-box domain and kinase domain. This changes the conformation of Plk1, making the Plk1 kinase domain accessible to Aurora A-mediated phosphorylation and activation. Activated Plk1 then phosphorylates its downstream targets and promotes mitotic entry (16). When cells encounter UV irradiation, ATR kinase is activated and phosphorylates Bora at Thr-501. This phosphorylation at the degron is recognized by β-TRCP, resulting in the degradation of Bora through the proteasome degradation system. Plk1 is thus kept inactivated, and mitotic entry is delayed. In summary, our studies reveal a key molecular mechanism by which the DNA damage signaling pathway regulates Plk1.

FIGURE 4.
A working model of DNA damage-induced G2/M checkpoint activation through the Bora-Plk1 pathway. Under normal, unstressed conditions, Bora accumulates in G2 phase, binds to Plk1, and facilitates Plk1 activation by Aurora A. Plk1 then activates its downstream ...

Supplementary Material

Supplemental Data:

*This work was supported, in whole or in part, by National Institutes of Health Grants CA130996, CA129344, and CA148940 (to Z. L.). This work was also supported by the Richard Schulze Family Foundation, by National Basic Research Program of China 973 Program Grant 2013CB530700), and by National Natural Science Foundation of China Grants 81222029 and 31270806 (to J. Y.).

An external file that holds a picture, illustration, etc.
Object name is sbox.jpgThis article contains supplemental Figs. S1 and S2.

3The abbreviations used are:

ATR
ataxia telangiectasia-mutated and Rad3-related
IR
ionizing radiation.

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