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Genome Announc. 2013 May-Jun; 1(3): e00264-13.
Published online 2013 May 23. doi:  10.1128/genomeA.00264-13
PMCID: PMC3662823

Complete Genome Sequence of Corynebacterium urealyticum Strain DSM 7111, Isolated from a 9-Year-Old Patient with Alkaline-Encrusted Cystitis

Abstract

Corynebacterium urealyticum is a common skin colonizer with potent urease activity. It is clinically recognized as an opportunistic pathogen causing urinary tract infections. The annotated genome sequence of strain DSM 7111, isolated from the urine of a young boy with an ectopic kidney, provides new insights into the pathomechanisms of this bacterium.

GENOME ANNOUNCEMENT

Corynebacterium urealyticum (formerly Corynebacterium group D2) is commonly isolated from the skin of hospitalized patients who are receiving broad-spectrum antibiotics (1). This bacterium has been well known for more than 30 years as an opportunistic pathogen causing mainly acute or encrusted cystitis, encrusted pyelitis, and pyelonephritis (1). It has also been detected as an uncommon pathogen in the urinary tract of small animals, such as cats and dogs (2, 3). The urease activity of C. urealyticum plays a key role in the pathogenesis of urinary tract infections and in the formation of struvite (magnesium ammonium phosphate) stones (4). In cases of chronic infections the medical treatment requires the administration of drugs and additional surgical intervention (1). The efficiency and outcome of the treatment are often affected by multiple resistances of C. urealyticum to a broad range of antibiotics (5). Antibiotic resistance genes detected in the genome of the clinical isolate C. urealyticum strain DSM 7109 are part of mobile DNA elements, which suggests that horizontal gene transfer is the main mechanism contributing to the development of multidrug resistance in this species (6).

Curealyticum DSM 7111 (ATCC 43040) was isolated from urine samples of a 9-year-old patient with an ectopic kidney (7). Genomic DNA was purified from an overnight culture as described previously (6). Genome sequencing was performed in a combined approach using the 454 Genome Sequencer FLX system (Roche Applied Science) and the Ion Torrent PGM Sequencer (Life Technologies). Pyrosequencing resulted in 799,528 reads and 80,374,097 detected bases, whereas semiconductor sequencing revealed 484,970 reads and 56,643,033 bases. The reads were assembled with the GS de novo Assembler (version 2.6) and the G4ALL software package (version 1.0.5) (http://g4all.sourceforge.net/) to yield 74 contigs sequenced with 59-fold coverage. The remaining gaps in the genome sequence were closed by PCR amplification and subsequent sequencing of the DNA fragments with an ABI 3730xl DNA Analyzer (Life Technologies). The deduced chromosome of C. urealyticum DSM 7111 has a size of 2,316,065 bp with an average G+C content of 64.24%. The manual annotation of the genome sequence was supported by the GenDB platform (8) and the REGANOR gene prediction server (9). Putative pseudogenes were identified with the CLC Genomics Workbench (CLC bio). Subsequent analysis of the sequence data followed established bioinformatic protocols for corynebacterial genomes (6). The annotation of the C. urealyticum DSM 7111 genome sequence revealed 1,935 protein-coding genes, 3 rRNA operons, and 54 tRNAs. The EDGAR software (10) detected 79 genes that are specific for C. urealyticum DSM 7111 and not present in the genome of the type strain C. urealyticum DSM 7109. Some of these strain-specific genes are clustered and encode a siderophore biosynthesis pathway, an iron ABC transport system, and a multicopper oxidase system, suggesting a different iron acquisition mechanism in C. urealyticum DSM 7111. Moreover, a putative phenylacetic acid degradation pathway might contribute to the virulence of C. urealyticum DSM 7111.

Nucleotide sequence accession number.

The genome project has been deposited in GenBank under the accession number CP004085.

ACKNOWLEDGMENTS

This genome project was supported by the CLIB Graduate Cluster Industrial Biotechnology. L.C.G. and S.C.S. acknowledge the receipt of a CAPES/DAAD and CAPES/PDSE scholarship.

We thank Susanne Götker (Bielefeld University) for performing PCR assays during the gap closure-phase of the project.

Footnotes

Citation Guimarães LC, Soares SC, Albersmeier A, Blom J, Jaenicke S, Azevedo V, Soriano F, Tauch A, Trost E. 2013. Complete genome sequence of Corynebacterium urealyticum strain DSM 7111, isolated from a 9-year-old patient with alkaline-encrusted cystitis. Genome Announc. 1(3):e00264-13. doi:10.1128/genomeA.00264-13.

REFERENCES

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Articles from Genome Announcements are provided here courtesy of American Society for Microbiology (ASM)