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Mol Cell Biol. 1988 November; 8(11): 4659–4668.
PMCID: PMC365555

Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts.


The phosphoprotein plastin was originally identified as an abundant transformation-induced polypeptide of chemically transformed neoplastic human fibroblasts. This abundant protein is normally expressed only in leukocytes, suggesting that it may play a role in hemopoietic cell differentiation. Protein microsequencing of plastin purified from leukemic T lymphocytes by high-resolution two-dimensional gel electrophoresis produced eight internal oligopeptide sequences. An oligodeoxynucleotide probe corresponding to one of the oligopeptides was used to clone cDNAs from transformed human fibroblasts that encoded the seven other oligopeptides predicted for human plastin. Sequencing and characterization of two cloned cDNAs revealed the existence of two distinct, but closely related, isoforms of plastin--l-plastin, which is expressed in leukocytes and transformed fibroblasts, and t-plastin, which is expressed in normal cells of solid tissues and transformed fibroblasts. The leukocyte isoform l-plastin is expressed in a diverse variety of human tumor cell lines, suggesting that it may be involved in the neoplastic process of some solid human tumors.

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Selected References

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  • Aebersold RH, Leavitt J, Saavedra RA, Hood LE, Kent SB. Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose. Proc Natl Acad Sci U S A. 1987 Oct;84(20):6970–6974. [PubMed]
  • Aebersold RH, Teplow DB, Hood LE, Kent SB. Electroblotting onto activated glass. High efficiency preparation of proteins from analytical sodium dodecyl sulfate-polyacrylamide gels for direct sequence analysis. J Biol Chem. 1986 Mar 25;261(9):4229–4238. [PubMed]
  • Cooper CS, Blair DG, Oskarsson MK, Tainsky MA, Eader LA, Vande Woude GF. Characterization of human transforming genes from chemically transformed, teratocarcinoma, and pancreatic carcinoma cell lines. Cancer Res. 1984 Jan;44(1):1–10. [PubMed]
  • Cooper HL, Feuerstein N, Noda M, Bassin RH. Suppression of tropomyosin synthesis, a common biochemical feature of oncogenesis by structurally diverse retroviral oncogenes. Mol Cell Biol. 1985 May;5(5):972–983. [PMC free article] [PubMed]
  • Dale RM, McClure BA, Houchins JP. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA. Plasmid. 1985 Jan;13(1):31–40. [PubMed]
  • Denhardt DT. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. [PubMed]
  • Goldman D, Goldin LR, Rathnagiri P, O'Brien SJ, Egeland JA, Merril CR. Twenty-seven protein polymorphisms by two-dimensional electrophoresis of serum, erythrocytes, and fibroblasts in two pedigrees. Am J Hum Genet. 1985 Sep;37(5):898–911. [PubMed]
  • Goldman D, Merril CR. Human lymphocyte polymorphisms detected by quantitative two-dimensional electrophoresis. Am J Hum Genet. 1983 Sep;35(5):827–837. [PubMed]
  • Goldman D, Merril CR, Polinsky RJ, Ebert MH. Lymphocyte proteins in Huntington's disease: quantitative analysis by use of two-dimensional electrophoresis and computerized densitometry. Clin Chem. 1982 Apr;28(4 Pt 2):1021–1025. [PubMed]
  • Goldstein D, Djeu J, Latter G, Burbeck S, Leavitt J. Abundant synthesis of the transformation-induced protein of neoplastic human fibroblasts, plastin, in normal lymphocytes. Cancer Res. 1985 Nov;45(11 Pt 2):5643–5647. [PubMed]
  • Goldstein D, Leavitt J. Expression of neoplasia-related proteins of chemically transformed HuT fibroblasts in human osteosarcoma HOS fibroblasts and modulation of actin expression upon elevation of tumorigenic potential. Cancer Res. 1985 Jul;45(7):3256–3261. [PubMed]
  • Gunning P, Mohun T, Ng SY, Ponte P, Kedes L. Evolution of the human sarcomeric-actin genes: evidence for units of selection within the 3' untranslated regions of the mRNAs. J Mol Evol. 1984;20(3-4):202–214. [PubMed]
  • Hendricks M, Weintraub H. Tropomyosin is decreased in transformed cells. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5633–5637. [PubMed]
  • Kondo I, Hamaguchi H. Evidence for the close linkage between lymphocyte cytosol polypeptide with molecular weight of 64,000 (LCP1) and esterase D. Am J Hum Genet. 1985 Nov;37(6):1106–1111. [PubMed]
  • Leavitt J, Bushar G, Kakunaga T, Hamada H, Hirakawa T, Goldman D, Merril C. Variations in expression of mutant beta actin accompanying incremental increases in human fibroblast tumorigenicity. Cell. 1982 Feb;28(2):259–268. [PubMed]
  • Leavitt J, Goldman D, Merril C, Kakunaga T. Changes in gene expression accompanying chemically-induced malignant transformation of human fibroblasts. Carcinogenesis. 1982;3(1):61–70. [PubMed]
  • Leavitt J, Gunning P, Kedes L, Jariwalla R. Smooth muscle alpha-action is a transformation-sensitive marker for mouse NIH 3T3 and Rat-2 cells. Nature. 316(6031):840–842. [PubMed]
  • Leavitt J, Kakunaga T. Expression of a variant form of actin and additional polypeptide changes following chemical-induced in vitro neoplastic transformation of human fibroblasts. J Biol Chem. 1980 Feb 25;255(4):1650–1661. [PubMed]
  • Leavitt J, Latter G, Lutomski L, Goldstein D, Burbeck S. Tropomyosin isoform switching in tumorigenic human fibroblasts. Mol Cell Biol. 1986 Jul;6(7):2721–2726. [PMC free article] [PubMed]
  • Leavitt J, Leavitt A, Attallah AM. Dissimilar modes of expression of beta- and gamma-actin in normal and leukemic human T lymphocytes. J Biol Chem. 1980 Jun 10;255(11):4984–4987. [PubMed]
  • Lin CS, Leavitt J. Cloning and characterization of a cDNA encoding transformation-sensitive tropomyosin isoform 3 from tumorigenic human fibroblasts. Mol Cell Biol. 1988 Jan;8(1):160–168. [PMC free article] [PubMed]
  • Maniatis T, Hardison RC, Lacy E, Lauer J, O'Connell C, Quon D, Sim GK, Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. [PubMed]
  • Matsumura F, Lin JJ, Yamashiro-Matsumura S, Thomas GP, Topp WC. Differential expression of tropomyosin forms in the microfilaments isolated from normal and transformed rat cultured cells. J Biol Chem. 1983 Nov 25;258(22):13954–13964. [PubMed]
  • Matsushima K, Shiroo M, Kung HF, Copeland TD. Purification and characterization of a cytosolic 65-kilodalton phosphoprotein in human leukocytes whose phosphorylation is augmented by stimulation with interleukin 1. Biochemistry. 1988 May 17;27(10):3765–3770. [PubMed]
  • Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. [PubMed]
  • Ohtsuka E, Matsuki S, Ikehara M, Takahashi Y, Matsubara K. An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions. J Biol Chem. 1985 Mar 10;260(5):2605–2608. [PubMed]
  • Paterson H, Reeves B, Brown R, Hall A, Furth M, Bos J, Jones P, Marshall C. Activated N-ras controls the transformed phenotype of HT1080 human fibrosarcoma cells. Cell. 1987 Dec 4;51(5):803–812. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Tabin CJ, Bradley SM, Bargmann CI, Weinberg RA, Papageorge AG, Scolnick EM, Dhar R, Lowy DR, Chang EH. Mechanism of activation of a human oncogene. Nature. 1982 Nov 11;300(5888):143–149. [PubMed]
  • Varma M, Aebi U, Fleming J, Leavitt J. A 60-kDa polypeptide in mammalian cells with epitopes related to actin. Exp Cell Res. 1987 Nov;173(1):163–173. [PubMed]
  • Witt DP, Brown DJ, Gordon JA. Transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus. J Cell Biol. 1983 Jun;96(6):1766–1771. [PMC free article] [PubMed]

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