Primary human B cells stimulated with T. cruzi produce IL-17
Purified human CD19+ tonsillar B cells were cultured for 72 hr in presence of recombinant IL-10 or BAFF and incubated with T. cruzi Tryp with or without α-Tsial mAb. B cells were also treated with Btk (AVL-292) or CD45 inhibitors or DMSO control. Production of (a) IL-17A or (b) IL-17F determined by ELISA. Data (3 replicates per condition) are shown as means ± s.e.m. *, P ≤ 0.05 **, P ≤ 0.005 (calculated by one-way ANOVA with Bonferroni correction). Results are representative of 3 independent experiments.