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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Nat Immunol. Author manuscript; available in PMC 2013 November 1.
Published in final edited form as:
Nat Immunol. 2013 May; 14(5): 514–522.
Published online 2013 April 7. doi: 10.1038/ni.2569

Figure 6

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Primary human B cells stimulated with T. cruzi produce IL-17

Purified human CD19+ tonsillar B cells were cultured for 72 hr in presence of recombinant IL-10 or BAFF and incubated with T. cruzi Tryp with or without α-Tsial mAb. B cells were also treated with Btk (AVL-292) or CD45 inhibitors or DMSO control. Production of (a) IL-17A or (b) IL-17F determined by ELISA. Data (3 replicates per condition) are shown as means ± s.e.m. *, P ≤ 0.05 **, P ≤ 0.005 (calculated by one-way ANOVA with Bonferroni correction). Results are representative of 3 independent experiments.

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