PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of elifeeLifeRecent contentAbout eLifeFor authorsSign up for alerts
 
eLife. 2013; 2: e00362.
Published online 2013 April 16. doi:  10.7554/eLife.00362
PMCID: PMC3628086

Acute stress enhances adult rat hippocampal neurogenesis and activation of newborn neurons via secreted astrocytic FGF2

Russ Fernald, Reviewing editor
Russ Fernald, Stanford University, United States;

Abstract

Stress is a potent modulator of the mammalian brain. The highly conserved stress hormone response influences many brain regions, particularly the hippocampus, a region important for memory function. The effect of acute stress on the unique population of adult neural stem/progenitor cells (NPCs) that resides in the adult hippocampus is unclear. We found that acute stress increased hippocampal cell proliferation and astrocytic fibroblast growth factor 2 (FGF2) expression. The effect of acute stress occurred independent of basolateral amygdala neural input and was mimicked by treating isolated NPCs with conditioned media from corticosterone-treated primary astrocytes. Neutralization of FGF2 revealed that astrocyte-secreted FGF2 mediated stress-hormone-induced NPC proliferation. 2 weeks, but not 2 days, after acute stress, rats also showed enhanced fear extinction memory coincident with enhanced activation of newborn neurons. Our findings suggest a beneficial role for brief stress on the hippocampus and improve understanding of the adaptive capacity of the brain.

DOI: http://dx.doi.org/10.7554/eLife.00362.001

Research organism: Rat

eLife digest

A little stress can be good for you. Just over 100 years ago, psychologists Robert Yerkes and John Dodson suggested that cognitive performance improves as stress increases, although it falls off again if stress levels continue to rise. The hippocampus is a key brain region for both memory and the regulation of emotion, and is highly sensitive to the main class of stress hormones, glucocorticoids. One particular subregion of the hippocampus, the dentate gyrus, contains a high density of glucocorticoid receptors, and is also notable for being one of only two regions in the adult mammalian brain that can give rise to new neurons via a process called neurogenesis.

Chronic stress is known to impair memory and to reduce neurogenesis. However, the effects of acute stress are less clear-cut: early studies suggested that it suppressed the generation of new neurons, whereas several recent studies have observed no effect. Other work has shown that acute stress increases the expression of growth factors—substances that stimulate cellular growth and proliferation—which would seem to suggest that stress could enhance neurogenesis.

Now Kirby et al. have obtained further insights into the effects of acute stress on the proliferation of cells in the dentate gyrus. Exposing rats to a moderate acute stressor, namely being restrained for a few hours, led to increased neurogenesis in the dorsal, but not ventral, hippocampus. Injecting rats with the stress hormone corticosterone had the same effect. In both cases, the enhanced neurogenesis was accompanied by increased expression of a growth factor called FGF2, which is produced by glial cells called astrocytes.

Intriguingly, Kirby and co-workers found that the stressed rats performed better than control animals in a memory test. Moreover, the beneficial effects were seen if the rats performed the task 2 weeks after their stressful experience, but not if they performed the task 2 days after being stressed. This is pertinent because new neurons in the dentate gyrus become functional 2 weeks after being generated, which suggests that the stress-induced increase in neurogenesis could account for the rats' improved memory.

The work of Kirby and co-workers has thus identified a mechanism by which moderate acute stress could have beneficial effects on cognition. Given that acute stress can be harmful in other instances—leading, for example, to post-traumatic stress disorder—further work is required to identify the factors that determine whether a response to stress is adaptive or pathological.

DOI: http://dx.doi.org/10.7554/eLife.00362.002

Introduction

Stress is a powerful and essential mediator of mammalian behavior. Proper response to a perceived stressor facilitates survival at the individual level and species propagation at the population level. Despite this necessity, stress responses can become maladaptive. Chronic stress, for example, leads to a host of adverse health consequences, including cardiovascular disease, obesity, depression, and exacerbation of neurodegeneration (McEwen, 2004). Acute stress, defined as a single exposure on the scale of minutes to hours without cycles of recovery and re-exposure, has proven more enigmatic.

One model of stress effects on the brain, an inverted U function, explains the variable consequences of acute stress for brain health (Lupien and McEwen, 1997). In this model, while severe or prolonged stressors are detrimental, brief or moderate stressors actually enhance neural function. Behavioral studies focusing on the memory functions of the hippocampus have demonstrated such a relationship in rodents, where moderate stress enhances memory performance yet more severe stress impairs it (Conrad et al., 1999).

The hippocampus is exquisitely sensitive to stress and the primary stress hormone class, glucocorticoids (GCs). Within the dentate gyrus (DG) sub-region, in particular, there exists a high density of GC receptors that respond to elevated circulating GCs (De Kloet et al., 1998). In addition, the DG is strongly connected via the entorhinal cortex and medial septum to the basolateral amygdala (BLA), a brain region involved in emotional processing and an important mediator of many stress effects on the hippocampus (McGaugh, 2004). Both of these mediators of stress (GCs and BLA input) have been shown to regulate the unique population of neural progenitor cells (NPCs) that reside in the adult DG (Kirby and Kaufer, 2009; Kirby et al., 2012b).

Dentate NPCs proliferate and give rise to new neurons throughout the lifespan in several mammalian species, including rats, mice and primates (Abrous et al., 2005; Kirby and Kaufer, 2009). They become electrophysiologically active, integrate into local circuitry, play important modulatory roles in hippocampal memory function (Abrous et al., 2005) and can respond to stress and stress hormones at multiple phases of development (Kirby and Kaufer, 2009). Moreover, recent work indicates that newborn, immature neurons integrate multiple signals more readily than mature neurons and that they have enhanced excitability, possibly contributing to a disproportionately large role in new memory formation (Marín-Burgin et al., 2012).

Numerous studies show that chronic stress, in addition to impairing memory function, suppresses proliferation, survival and differentiation of new neurons in the adult DG (Wong and Herbert, 2004; Mirescu and Gould, 2006; Kirby and Kaufer, 2009). The effect of acute stress on neurogenesis, however, is unclear. While early work indicated suppression of proliferation following acute stress or GC injection (Cameron and Gould, 1994; Gould et al., 1997), subsequent studies have yielded mixed results, often reporting no change in proliferation following a variety of acute stressors (Thomas et al., 2006; Thomas et al., 2007; Dagyte et al., 2009; Hanson et al., 2011). In contrast, investigations of hippocampal growth factor secretion have shown that acute stress enhances expression of mitogenic growth factors such as basic fibroblast growth factor (FGF2) and nerve growth factor (NGF) (Mocchetti et al., 1996; Molteni et al., 2001), implying a potential for increased neurogenesis following acute stress. Indeed, a number of interventions that stimulate GC release such as acute exercise (Kronenberg et al., 2006) and acute sexual experience (Leuner et al., 2010) actually increase neurogenesis in the adult hippocampus. Combined, these studies suggest that adult hippocampal neurogenesis may follow an inverted U function similar to hippocampal memory—decreasing following chronic stress yet increasing in response to acute stressors.

We examined the effect of several forms of acute stress on adult hippocampal neurogenesis, seeking to resolve the contradictory evidence for enhanced vs impaired hippocampal plasticity. We found that acute stress or corticosterone (CORT, the primary rat GC) administration increased dorsal but not ventral hippocampus cell proliferation in adult rats. This increase was not dependent on input from the BLA and was accompanied by an increase in FGF2 expression in dorsal hippocampal astrocytes. Furthermore, we show that astrocyte-secreted FGF2 is necessary for CORT-induced enhancement in NPC proliferation in vitro. 2 weeks after acute stress, when newborn neurons are first becoming functional, we also find enhancement in hippocampus-dependent memory accompanied by enhanced activation of newborn neurons. These findings have important implications for understanding regulation of hippocampal plasticity in the face of environmental challenge and in distinguishing adaptive vs pathological stress responses. Moreover, they suggest that stress effects on adult neurogenesis may follow an inverted U function similar to that already demonstrated for hippocampal memory function (Lupien and McEwen, 1997).

Results

Acute stress increases dorsal hippocampus NPC proliferation

To investigate the effect of acute stress on adult neurogenesis in the DG (Figure 1), we chose three common models of acute stress in rodents: 30 min novel environment, 30 min footshock or 3 hr immobilization. Rats were handled for 5 days prior to stress exposure then perfused 3 hr after the beginning of the stressor (Figure 2A). Immobilization stress significantly increased the number of cells immunopositive for the proliferation marker Ki67 in the dorsal DG 3.23-fold above control (27.51 ± 2.74 control vs 88.99 ± 12.49 immob) while novel environment or footshock did not significantly alter Ki67+ count compared to control (Figure 2B,C). Plasma CORT was significantly elevated above control levels both 30 min and 3 hr after stressor initiation in immobilized rats but not in novel environment- or footshock-exposed rats (Figure 2D). This finding suggested that an increase in CORT might underlie the immobilization-induced increase in DG proliferation. To test that hypothesis, we next habituated handled rats to daily oil injections for 3 days, injected them with exogenous CORT (0, 5 or 40 mg/kg body weight) and assessed cell proliferation 3 hr later (Figure 2A). 40 mg/kg CORT significantly increased the number of Ki67+ proliferative cells in the dorsal DG 1.92-fold above oil-injected controls (26.19 ± 2.83 0 mg/kg CORT vs 50.29 ± 7.74 40 mg/kg CORT; Figure 2C,E). Plasma CORT levels were also consistently elevated 30 min and 3 hr after injection (Figure 2F), similar to the levels seen in immobilized rats. Notably, injection of 5 mg/kg CORT yielded similar plasma CORT levels to footshock (approximately 119 and 121 ng/ml, respectively) and also did not produce an increase in the numbers of Ki67+ cells. If rats were not habituated to injection, no difference in cell proliferation was found (Figure 2J,K). We next assessed short-term survival of newly-born cells following acute stress. 3 hr after the start of immobilization or CORT injection, rats were injected with the proliferative marker 5-bromodeoxyuridine (BrdU) then perfused 24 hr later. Both immobilization (Figure 2G,I) and 40 mg/kg CORT injection (Figure 2H,I) significantly increased the number of cells immunopositive for BrdU surviving 24 hr after termination of the stressor in the dorsal DG by 1.92 and 1.48-fold, respectively (34.88 ± 8.70 control vs 68.96 ± 10.23 immob; 39.80 ± 6.89 0 mg/kg CORT vs 58.95 mg/kg CORT).

Figure 1.
Dorsal versus ventral DG.
Figure 2.
Acute stress increases adult cell proliferation in dorsal hippocampus.

Acute stress does not increase ventral hippocampus NPC proliferation

Recent work suggests different functional roles for the dorsal and ventral hippocampus in mediating spatial memory vs emotion regulation, respectively (Fanselow and Dong, 2010). Adult neurogenesis may also be differentially regulated in dorsal vs ventral hippocampus; while environmental enrichment enhances neurogenesis in both dorsal and ventral DG, chronic mild stress preferentially suppresses proliferation in the ventral subregions (Tanti et al., 2012). In our acute stress models, we found no effect of novel environment, footshock, immobilization or CORT injection (Figure 3A–C) on proliferative Ki67+ cell number in the ventral hippocampus (Figure 1B).

Figure 3.
Acute stress does not increase adult cell proliferation in ventral hippocampus.

BLA input does not modulate acute stress-induced proliferation

Work by McGaugh and colleagues shows that acute stress-induced alterations in hippocampal memory and long-term potentiation depend on input from the BLA (Quirarte et al., 1997; Roozendaal et al., 1999; Roozendaal et al., 2009b), a fear and emotion processing center. In addition, we have previously shown that BLA input regulates adult DG neurogenesis under basal conditions and is required for the activation of newborn neurons in a fear conditioning paradigm (Kirby et al., 2012b). To test whether BLA input is necessary for the acute stress-induced increase in proliferation, we performed unilateral excitotoxic lesions of BLA in adult rats (as described in [Kirby et al., 2012a, 2012b]). Animals were then exposed to immobilization stress or no stress control (Figure 4A). At the end of the stressor, each rat received a BrdU injection and was perfused 2 hr later. Plasma CORT response to immobilization was similar in lesioned and sham-operated rats (Figure 4B), suggesting intact hormonal stress response in BLA-lesioned, immobilized rats. Consistent with our findings in intact rats, sham-operated immobilized rats had a significant 2.6-fold increase in BrdU+ proliferative cells over no stress controls (6.37 ± 1.14 control vs 16.69 ± 2.11 immob; Figure 4C). BLA lesion suppressed proliferation ipsilateral to the lesion by approximately 1.5-fold (1.66-fold control, 1.44-fold immob), as we have previously reported, but did not block the stress-induced increase in proliferation (Figure 4D). These findings suggest that input from the BLA is not necessary for acute stress effects on adult hippocampal cell proliferation.

Figure 4.
Acute stress increases cell proliferation independent of BLA input.

CORT-induced increase in isolated NPC proliferation is dependent on astrocyte signaling

Given that the stress-induced increase in proliferation was not dependent on BLA input, we next tested whether stress-induced proliferation is a cell autonomous phenomenon. This was accomplished by quantifying the response of isolated adult rat hippocampal NPCs grown in vitro (as described in Gage, 2000) to acute CORT exposure. NPCs were FGF2 deprived for 24 hr then treated with 1 μM CORT (equivalent to approximately 350 ng/ml, the level measured in plasma of immobilized rats) or EtOH vehicle in either high (20 ng/ml) or low (0 ng/ml) FGF2. After 3 hr, BrdU was added to the cells and they were fixed 2 hr later (Figure 5A). Cultured rat hippocampal NPCs depend on FGF2 signaling for proliferation (Chipperfield et al., 2002). We found that while high FGF2 increased the percentage of proliferating BrdU+ NPCs over low FGF2 (1.21-fold in EtOH, 1.48-fold in 1 μM CORT), exposure to CORT did not affect the percent of proliferative cells in either high or low FGF2 media as compared to vehicle (Figure 5B). These data indicate that NPCs do not respond to acute CORT in isolation, but likely rely on input from another cell type in the neurogenic niche. A growing body of work indicates that astrocytes can strongly regulate NPC dynamics through secreted factors (Song et al., 2002), so we next tested whether astrocytes might participate in the regulation of NPCs in response to CORT. We treated primary hippocampal astrocytes cultured as described in (McCarthy and de Vellis, 1980) with 1 μM CORT or EtOH vehicle for 3 hr then extracted astrocyte conditioned media (ACM). When administered to isolated NPCs, CORT-treated ACM increased the percent of BrdU+ NPCs significantly over EtOH-treated coculture media (CoC) control by 1.52-fold (29.56 ± 2.81 CoC-EtOH vs 44.93 ± 2.82 ACM-1 μM CORT; Figure 5C,D). These findings suggest that astrocytes could mediate the in vivo increase in NPC proliferation following acute stress through secreted factors.

Figure 5.
ACM from CORT-treated astrocytes increases NPC proliferation.

Acute stress increases dorsal hippocampus FGF2 expression

Given that astrocytes secrete a variety of growth factors that support cell proliferation, we next investigated hippocampal growth factor expression in stressed rats. We quantified levels of the following growth factors previously reported to be mitogenic and/or elevated by acute stress (Mocchetti et al., 1996; Molteni et al., 2001; Ma et al., 2009) in the dorsal hippocampus of stressed and control rats: FGF2, brain derived neurotrophic factor (BDNF), nerve growth factor (NGF), FGF receptor 1 (FGFR1), FGFR2, FGFR3, FGFR4, vascular endothelial growth factor (VEGF), growth arrest and DNA damage–inducible 45β (GADD45β) as well as the early immediate gene, CFOS. Acute stress caused a significant increase in FGF2 mRNA and protein levels in the dorsal hippocampus (Figure 6A–D,I). Immobilization increased fgf2 mRNA 1.77 (±0.20) fold over control while 40 mg/kg CORT increased fgf2 mRNA by 1.70 (±0.23) fold over 0 mg/kg CORT (Figure 6A,B). Immobilization and 40 mg/kg CORT also significantly increased FGF2 protein levels in the dorsal hippocampus by 1.59 (±0.24) fold and 2.54 (±0.34) fold, respectively (Figure 6C,D,I). In contrast, bdnf exon IV mRNA levels were significantly decreased by immobilization and CORT (Figure 6E,F). BDNF protein level, however, was unchanged by either manipulation (Figure 6G–I). Acute stress did not consistently alter mRNA levels of ngf, fgfr1, fgfr2, fgfr3, fgfr4, vegf, bdnf exon IX, or gadd45β (Figure 7A–P). mRNA of the immediate early gene cfos was generally increased at 30 min after any manipulation, including oil injection (Figure 7Q,R).

Figure 6.
Acute stress increases FGF2 expression in dorsal hippocampus.
Figure 7.
mRNA expression levels in dorsal hippocampus following acute stressors.

Acute stress does not increase FGF2 expression in the ventral hippocampus

We next quantified FGF2 expression in ventral DG where we previously saw no effect of stress on cell proliferation. Immobilization and 40 mg/kg CORT injections significantly increased fgf2 mRNA levels in the ventral hippocampus, similar to the dorsal hippocampus (Figure 6J,K). However, protein levels of FGF2 were not increased by any of the acute stressors in the ventral portion of the hippocampus (Figure 6L–N). These results provide intriguing correlative evidence that increased FGF2 protein levels following acute immobilization or CORT exposure could underlie the observed increase in dorsal hippocampal proliferation.

Acute stress increases FGF2 expression in hilar astrocytes of the dorsal hippocampus

To determine whether astrocytes were the source of increased dorsal hippocampal FGF2 in vivo, we used confocal microscopy to quantify double immunohistochemical labeling for FGF2 and the astrocyte marker, glial fibrillary acidic protein (GFAP) in adult male rats treated as in Figure 2A. Consistent with previous reports (Bhatnagar et al., 1997), FGF2-immunoreactive cells were found throughout the DG and the hilus, with staining primarily in the cell body and nucleus. The DG is primarily composed of granule neurons and while almost every cell expressed FGF2, we found very few GFAP+ cells within the DG as expected. Mean optical density (with background correction) throughout the Z-stack of FGF2 expression in the DG revealed no effect of stress or CORT injection on DG FGF2 expression (Figure 8A,D). In the hilus, a mixture of GFAP+ and GFAP- cells was found, with almost all GFAP+ cells being FGF2+. By quantifying integrated optical density of individual FGF2+ cells that were either GFAP+ or GFAP−, we found that both immobilization and 40 mg/kg CORT injection increased FGF2 signal in GFAP+ cells significantly over their respective control groups (Figure 8B,D). No effect of immobilization or CORT was observed in GFAP− cells in the hilus (Figure 8C,D). These findings suggest that the stress-induced increase in FGF2 levels in the DG most likely comes from neighboring astrocytes in the hilus.

Figure 8.
Acute stress increases FGF2 expression in GFAP+ astrocytes in the dorsal hilus.

FGF2 neutralization in CORT-ACM blocks increased NPC proliferation

To determine whether FGF2 was the astrocyte-derived factor driving NPC proliferation, we next examined the role of FGF2 in the effect of CORT-treated ACM on isolated NPCs. Consistent with previous work (Forget et al., 2006), media from untreated astrocytes showed no detectable FGF2 (0.12 ± 0.46 pg/ml). In contrast, astrocytes treated with 1 μM CORT for 3 hr secreted 3.5 ± 0.68 pg/ml FGF2 protein (Figure 9A). We next tested whether levels of FGF2 as low as 4 pg/ml were sufficient to stimulate NPC proliferation. Treating with 4 pg/ml rat recombinant FGF2 caused a significant 1.39-fold increase in BrdU labeling compared to vehicle (0 pg/ml FGF2), suggesting that the levels of FGF2 present in CORT-ACM were sufficient to stimulate NPC proliferation (11.95 ± 1.22% BrdU+, 0 pg/ml; 16.65 ± 1.72% BrdU+, 4 pg/ml, p=0.04). We next investigated whether blocking FGF2 function could prevent acute stress-induced enhancement of NPC proliferation using a neutralizing antibody against FGF2 (nAb). An ELISA for rat FGF2 revealed that the nAb decreased available FGF2 over a wide range of concentrations (Figure 9B). The nAb did not, however, affect the availability of FGF1 (Figure 9C), a closely related member of the FGF family. When tested in vitro, we found that FGF2 neutralization blocked FGF2-induced stimulation of NPC proliferation without affecting proliferation in FGF2-free conditions (Figure 9D). These findings suggest that the nAb blocks FGF2 signaling specifically and does not have nonspecific toxic effects on NPC growth. We then pretreated ACM from EtOH- or CORT-treated astrocytes with FGF2 nAb before exposing NPCs to the treated media. Pretreatment with the FGF2 nAb blocked the CORT-ACM induced increase in NPC proliferation (30.67 ± 3.44% BrdU positive in EtOH, no nAb vs 30.33 ± 2.59% BrdU positive in 1 μM CORT with nAb; Figure 9E), suggesting that increased astrocytic FGF2 is the driving signal for CORT-ACM-induced proliferation of adult hippocampal NPCs.

Figure 9.
Blocking FGF2 prevents CORT-ACM induced increase in NPC proliferation.

Acute stress leads to a delayed enhancement of fear extinction retention

Several studies suggest that newly-born neurons play an important functional role in hippocampal memory during an immature, highly plastic phase of their development (Kee et al., 2007; Deng et al., 2009; Kitamura et al., 2009; Stone et al., 2010). However, newborn neurons in the adult rat require two or more weeks to mature and become physiologically active (Snyder et al., 2009), implying that the enhancement in neurogenesis we observed following acute stress should require several weeks to influence behavior. We therefore assessed hippocampal memory using a fear conditioning task either 2 days or 2 weeks after acute stress. Immobilized and control rats were given 10 unsignaled, 1 s, 1 mA shocks in a fear conditioning chamber. The next day, they received five 10 min extinction trials where they were exposed to the fear conditioning chamber without shock. On the third day of testing, they received a single 10 min extinction probe trial without shock. When rats were tested 2 days after immobilization, control and immobilized rats showed similar freezing behavior during training, extinction and the 24 hr extinction probe (Figure 10A–C). When rats were tested 2 weeks after immobilization, immobilized rats did not differ from controls in percent time freezing during training or during the five extinction trials (Figure 10D,E). During the extinction probe, however, immobilized rats froze significantly less than control rats, indicating better retention of the fear extinction (Figure 10F). These results suggest that an incubation time is necessary for acute immobilization to improve fear memory and demonstrate enhanced hippocampal memory at a time when newborn neurons are highly plastic and sensitive to environmental input.

Figure 10.
Acute stress causes delayed enhancement of contextual fear extinction retention.

Acute stress leads to enhanced activation of newly born neurons

To determine if the neurons born 2 weeks before fear conditioning might play a role in the enhancement of fear memory retention, we quantified cell fate and activation in a subset of rats perfused 1 hr after the fear extinction probe. The number of surviving BrdU+ cells (Figure 10G) and the percent of BrdU+ cells expressing the immature neuronal marker doublecortin (DCX+) or the astrocytic marker GFAP+ (Figure 10H) were similar in immobilized and control rats. However, immobilized rats had significantly more immature (DCX+) cells expressing the immediate early gene cfos than control rats (1.57 ± 0.52% cfos+ vs 5.39 ± 1.40% cfos+; Figure 10I,J), suggesting greater activation of immature neurons in immobilized rats. These results indicate enhanced utilization of the highly plastic pool of new neurons born around the time of an acute stressor and suggest that stress-stimulated proliferation may support later memory benefits.

Discussion

The present study demonstrates that acute stress or exposure to the stress hormone CORT induces an increase in proliferation of hippocampal NPCs via increased secretion of astrocytic FGF2. This increase in proliferation is correlated with selective activation of the hyper-plastic newborn neurons and enhanced retention of fear extinction 2 weeks after the stressor. Taken together, these findings suggest a beneficial role for acute stress on hippocampal plasticity. Consistent with our findings, previous studies using similar stress paradigms show stress-induced enhancements in memory consolidation and growth factor expression (Roozendaal et al., 1999; Molteni et al., 2001; Roozendaal et al., 2009b). Acute stress may also support hippocampal LTP, another factor implicated in stimulating adult hippocampal neurogenesis (Korz and Frey, 2005). Notably, other manipulations that increase stress hormone secretion, such as exercise, sexual experience and mild immune challenge, similarly stimulate adult neurogenesis (Kronenberg et al., 2006; Wolf et al., 2009; Leuner et al., 2010; Buwalda et al., 2012). Recent work in adult squirrel monkeys also shows that coping with intermittent social stress through multiple pair separations and new pair formations both stimulates adult hippocampal neurogenesis and improves hippocampal memory (Lyons et al., 2010). These studies, along with our findings, evoke the interesting possibility that acute stress may be beneficial for brain health in general and hippocampal plasticity in particular.

Previous studies of adult NPC response to acute stress have yielded mixed results. While some initial work suggested that acute stress causes a decrease in NPC proliferation (Cameron and Gould, 1994; Gould et al., 1997), more recent publications have not replicated these findings (Thomas et al., 2006; Thomas et al., 2007; Dagyte et al., 2009; Hanson et al., 2011). Notably, while we found that acute CORT enhanced proliferation in well-handled, habituated rats, we did not find any effect of acute CORT on neurogenesis in rats that were not habituated to injection. Our findings therefore demonstrate that factors present before the stressor begins may alter the effect of acute stress, perhaps by changing the susceptibility of controls or stressed animals to manipulation. Not surprisingly, a rich literature exists regarding the role of handling on altering the rodent stress response (Korosi and Baram, 2010). Such experimental differences could explain discrepancies in the literature.

Previous research concerning the long-term effects of acute stress in rodents focuses primarily on models of traumatic stress and the resultant PTSD-like symptoms. Most prominently, the single prolonged stress model of PTSD uses three acute stressors in series (restraint, forced swim and ether) and results in delayed deficits in fear extinction, as well as enhanced anxiety (Knox et al., 2012a, 2012b). However, if any one of the three components of this stressor protocol are eliminated, deficits are no longer evident (Knox et al., 2012a), suggesting that stressor effects depend on the severity and length of the stressor, perhaps following the inverted U function previously described (Lupien and McEwen, 1997).

Previous work in primates has provided additional evidence for an inverted U relationship between stress and neural function. In multiple non-human primate species, for example, mild early life stress can lead to resilience against stress later in life, a phenomenon referred to as stress inoculation (Lyons and Parker, 2007; Katz et al., 2009; Parker and Maestripiero, 2011). In contrast, more severe early life stress can have the opposite outcome, increasing stress vulnerability later in adulthood. When combined with our current findings, these studies fit well with the proposed inverted U function for stress effects on brain health (Lupien and McEwen, 1997; Salehi et al., 2010; Luksys and Sandi, 2011). At high, traumatic levels, acute stress may result in maladaptive pathology (e.g., PTSD-like symptoms in the single prolonged stress model). At more moderate levels, though, such as immobilization in well-handled rats, acute stress may actually enhance function. Future research will be needed to more precisely define the limits of stimulating vs detrimental acute stress.

Many acute-stress-induced changes in hippocampal plasticity rely on functional input from the BLA. For example, while stress enhances hippocampal memory consolidation and LTP, lesion of the BLA blocks these enhancements (Quirarte et al., 1997; Roozendaal et al., 2009a, 2009b). We have recently reported that hippocampal cell proliferation and the activation of the newborn neurons in a fear-conditioning paradigm both depend on BLA neural input (Kirby et al., 2012b). In the current study, we found that while BLA lesion suppressed hippocampal cell proliferation as we have shown before, it did not affect the acute stress-induced increase in neurogenesis. These findings imply that acute stress regulation of neurogenesis may not rely on the same systems-level in vivo circuitry that mediates acute stress regulation of memory consolidation and LTP. In support of this hypothesis, we were able to model CORT-induced NPC proliferation in isolated NPCs. We found that while NPCs did not respond to acute CORT treatment independently in vitro, they did proliferate more in response to conditioned media from CORT-treated primary astrocytes, suggesting a role for secreted factors from local astrocytes in mediating stress effects on NPCs.

The dynamic role of astrocytes in facilitating neuronal function through secreted factors has gained much recognition (Eroglu and Barres, 2010). In addition to aiding in synaptic glutamate recycling, astrocytes secrete several factors such as thrombospondins (Eroglu and Barres, 2010), Hevin, SPARC (Kucukdereli et al., 2011) and glypicans (Allen et al., 2012) that regulate synaptic formation and function in mature neurons (Eroglu and Barres, 2010). Astrocytes express the glucocorticoid receptor, GR, and when they are exposed to high levels of GCs, GC-bound GR translocates to the nucleus and enhances FGF2 gene transcription (Molteni et al., 2001; Gubba et al., 2004; Unemura et al., 2012). FGF2 is a potent and necessary proliferative factor in adult NPCs (Chipperfield et al., 2002). We found that acute stress stimulated FGF2 expression in the dorsal hippocampus and in primary hippocampal astrocytes. We further showed that in the DG and hilus, the enhancement in FGF2 levels following stress is largely restricted to GFAP+ astrocytes. Notably, changes in FGF2 protein levels following acute stress closely paralleled the neurogenic response; that is, FGF2 levels were increased only in stress conditions that also stimulated neurogenesis. Neutralizing the astrocyte-secreted FGF2 prevented enhanced proliferation in cultured NPCs. These findings suggest a novel role for astrocytes in supporting hippocampal plasticity in response to an environmental stressor through secreted FGF2. Further research will be required to fully dissect the molecular mechanisms by which stress induces FGF2 secretion from astrocytes.

Newly-born neurons are implicated in numerous hippocampal memory functions (Aimone et al., 2010), particularly contextual fear conditioning. Contextual fear conditioning activates immature neurons (Kirby et al., 2012b) and when new neurons are selectively knocked down, fear extinction is impaired (Deng et al., 2009; Stone et al., 2010). Sahay et al. have further shown that prolonging survival of newly-born neurons through targeted knockdown of apoptotic pathways enhances discrimination between similar contexts in a fear conditioning task (Sahay et al., 2011). We found that 2 weeks, but not 2 days, after acute stress, immobilized rats showed enhanced contextual fear extinction retention compared to controls. We also found that immobilized rats had greater activation of newly born neurons in response to fear extinction recall. This time window coincides with the period in which the newborn cells are hyper-plastic and more likely to be recruited to active circuitry (Kee et al., 2007; Deng et al., 2009; Kirby et al., 2012b). Given that immobilized rats had a similar number of newborn neurons as controls, these data suggest that immobilized rats better utilize the pool of immature neurons, possibly contributing to their enhanced memory. However, this connection does not preclude the potential contribution of other aspects of hippocampal plasticity to the enhanced memory. Future research will be necessary to fully determine the role of immature neurons vs existing circuitry in enhancement of hippocampal memory.

A recent hypothesis posits that the dorsal and ventral hippocampus support different behavioral functions, with the dorsal region being important for spatial and declarative memory while the ventral region facilitates affective regulation (Fanselow and Dong, 2010). This division may also apply to neural stem cell regulation within the DG of each region where chronic stress preferentially effects the ventral over the dorsal hippocampus, perhaps reflecting the negative affective consequences of chronic stress (Tanti et al., 2012). In the present study, we found that acute stress or stress hormone exposure increased neurogenesis and FGF2 expression in the dorsal, but not in the ventral, hippocampus. Moreover, the memory benefits observed 2 weeks after stress were in contextual fear extinction, a task that relies on the kind of spatial memory hypothesized to depend on the dorsal DG (Fanselow and Dong, 2010). This selective involvement of the dorsal hippocampus implies that our acute stressor model functions as a cognitive stimulant for the declarative domains of hippocampal function rather than as a modulator of emotional responsivity.

The brain's response to acute stress can define the line between life-saving adaptation and long-term pathology. The current study suggests that moderate, acute stress may stimulate heightened brain plasticity via increased neurogenesis. These findings have important implications for understanding adaptive vs pathological responses to stress.

Materials and methods

Animals

Adult male Sprague-Dawley rats (Charles River) were pair-housed on a 12 hr light dark cycle with lights on at 07:00 hr. Rats were allowed to acclimate to the animal facility for 1 week before handling began. All animal procedures were approved by the UC Berkeley Animal Care and Use Committees.

Stressors

Novel environment, footshock, immobilization and control rats were all handled for 5 days. On the sixth day, they were exposed to a stressor or left undisturbed in the case of controls. The novel environment and footshock exposure both lasted 30 min and occurred in fear conditioning chambers described in the ‘Fear conditioning'. For footshock, rats were exposed to 1 mA, 1 s duration unsignaled shock 30 times. Rats were returned to their home cage after the 30 min exposure until the time of sacrifice. Immobilized rats were confined for 3 hr in decapicone bags (Braintree Scientific, Braintree, MA).

Corticosterone injection

For most experiments, rats were handled for 2 days, given a subcutaneous (SC) needle stick on day 3 and then injected with sesame oil (SC) for 2 days (days 4 and 5). On the sixth day, rats received 0, 5 or 40 mg/kg corticosterone (SC, Sigma, St. Louis, MO) suspended in sesame oil. CORT was either at 5 mg/ml or 40 mg/ml such that rats receiving 5 or 40 mg/kg corticosterone received equal volumes of oil relative to body weight. In the case of the rats not pre-injected, rats were handled for 3 days and then injected with 0, 5 or 40 mg/kg corticosterone as above on the fourth day.

BrdU injection

5-Bromo-2′-deoxyuridine (BrdU, Sigma) was dissolved in physiological saline. Rats were injected with BrdU (intraperitoneally, 200 mg/kg) 3 hr after the beginning of the stressor or injection unless otherwise noted.

BLA lesion

Excitotoxic lesions of the BLA were performed using unilateral stereotaxic infusion of N-methyl-d-aspartate (Sigma) as per (Kirby et al., 2012a, 2012b). Coordinates for BLA infusion were: −2.8 mm anterior/posterior (A/P), ±5.1 mm medial/lateral (M/L) relative to bregma; −6.8 mm (2 min) and −6.5 mm (1 min) relative to dura. Following 3 weeks of recovery, during which rats were handled regularly, rats were immobilized for 3 hr (n = 7 each, sham and unilateral lesion) or left undisturbed in their home cage (n = 7 each, sham and unilateral lesion, respectively). Tail vein blood samples were taken at the beginning and end of immobilization for plasma corticosterone quantification. At the end of immobilization, all rats received one injection of 100 mg/kg BrdU and were perfused 2 hr later.

Immunohistochemical staining

Rats were anesthetized with Euthasol euthanasia solution and transcardially perfused with ice cold 0.1 M phosphate buffered saline (PBS) followed by 4% paraformaldehyde in 0.1 M PBS. Brains were post-fixed for 24 hr at 4°C, equilibrated in 30% sucrose in 0.1 M PBS and then stored at −20°C. Immunostaining was performed on a 1 in 6 series of free-floating 30 µm cryostat sections.

Ki67 staining was done for sections from control (n = 10), novel environment (n = 6), footshock (n = 4), immobilized (n = 6), 0 mg/kg (n = 6), 5 mg/kg (n = 6) and 40 mg/kg (n = 6) CORT-injected rats as per (Kirby et al., 2012b) with a few additions. Sections were antigen-retrieved using 10 mM citrate buffer, pH 8.0 at 95°C for 20 min prior to peroxidase blocking and the primary antibody used was rabbit anti-Ki67 (1:500; Novus, St. Louis, MO).

BrdU staining was done as per (Kirby et al., 2012b) for sections from control (n = 6), immobilized (n = 5), 0 mg/kg (n = 6), and 40 mg/kg (n= 5) rats who were perfused 24 hr after the BrdU injection, which occurred 3 hr after the start of the stressor. Sections from control (n = 10) and immobilized (n = 8) rats who were perfused 2 weeks after immobilization/BrdU injection, 1 hr after the final fear extinction probe (see ‘Fear conditioning'), were also stained for BrdU using the same procedure. All stained BrdU and Ki67 sections were mounted on gelatin-coated slides, dehydrated in alcohols and coversliped with permount.

Double immunohistochemical labeling for FGF2 quantification in GFAP+ cells was done on sections from control (n = 2), immobilized (n = 6), 0 mg/kg CORT injected (n = 6) and 40 mg/kg CORT injected (n = 6) rats as per (Kirby et al., 2012b) with the following deviations. Primary antibodies were rabbit anti-FGF2 (1:500; Abcam, Cambridge, UK), and mouse anti-GFAP (1:1000; Cell Signalling, Danvers, MA). Secondary antibodies were AlexaFluor 488 donkey anti-rabbit (1:200; Invitrogen, Carlsbad, CA) and Cy3 donkey anti-mouse (1:200; Jackson ImmunoResearch, West Grove, PA). Following secondary incubation and rinsing, sections were mounted on gelatin-coated slides and coverslipped with Vectashield mounting medium with DAPI (Vector, Burlingame, CA).

Triple immunohistochemical labeling for cell fate analysis was done on sections from control (n = 6) and immobilized (n = 6) rats 2 weeks after immobilization/BrdU injection, after the final fear extinction probe (see ‘Fear conditioning') as per (Kirby et al., 2012b) with a few exceptions. Primary antibodies were goat anti-DCX (1:200; Santa Cruz Biotechnology, Dallas, TX), mouse anti-GFAP (1:100; Cell Signalling) and rat anti-BrdU (1:500; Abcam). Secondary antibodies were AlexaFluor 594 anti-goat, AlexaFluor 647 anti-mouse and biotin anti-rat (1:500; Jackson ImmunoResearch). Tertiary antibody was Streptavidin Alexa Fluor 488 (1:1000; Jackson ImmunoResearch). Double immunohistochemical staining for DCX and cfos was performed similarly, (n = 5 control, n = 7 immobilized) with goat anti-DCX as above and mouse anti-cfos (1:50; Santa Cruz Biotechnology). Secondary antibodies were as above for anti-goat and AlexaFluor 647 anti-mouse (1:500; Jackson ImmunoResearch). Sections were mounted on gelatin-coated slides and coversliped with DABCO antifading medium.

Quantification of Ki67+ and BrdU+ cells

Ki67- and BrdU-positive cells were counted in the dorsal and ventral dentate gyrus and subgranular zone using a 40× air objective (Zeiss, Oberkochen, Germany). The area sampled was calculated using StereoInvestigator software (MicroBrightfield, Williston, VT) and used to calculate the number of positive cells per micrometer (Lupien and McEwen, 1997).

Confocal analysis

For quantification of FGF2 immunoreactivity, 18 μm Z-stacks of 1 μm slices in the dorsal DG and hilus were acquired using a 20× air objective. Mean DG optical density was measured in ImageJ software using the summed Z-stack of FGF2 immunoreactivity. Mean optical density of FGF2- areas of tissue were subtracted from the DG intensity value to correct for background. Integrated optical density of GFAP+ and GFAP- negative cells in the hilus were determined by confirming GFAP expression in the Z-stack and then acquiring integrated optical density from the summed Z-stack of each individual cell. 51 to 97 GFAP+ cells and 16 to 57 GFAP- cells were sampled per rat. To quantify cFos expression in new neurons, BrdU positive cells were located in the dorsal dentate gyrus for each animal using a 40× oil objective and assessed in z-series of <1.0 μm slices to determine if other markers (DCX, GFAP) were co-expressed. Confocal images were captured on a Zeiss 510 META/NLO confocal microscope with a 40× oil objective.

Real time quantitative PCR

Rats were lightly anesthetized with isoflurane and rapidly decapitated 30 min or 3 hr after the beginning of their respective stressors (n = 6/grp). Bilateral hippocampi were dissected and flash frozen in liquid nitrogen. Trunk blood was collected for plasma corticosterone quantification. One hippocampus per rat was used for mRNA expression quantification. The other was used for western blot analysis, right and left side being counterbalanced among groups. Real-time reverse transcriptase PCR was run on Trizol-extracted RNA with primers detailed in Table 1.

Table 1.
List of primers

Primer sequences were designed using Primer1 software and checked for specificity using BLAST. Extracted RNA was treated with DNase (DNA-free, Ambion, Carlsbad, CA), and two-step PCR was used, following manufacturer instructions for iScript cDNA synthesis kit (BioRad, Hercules, CA) and then iQ SYBR Green Supermix. Samples were run in a BioRad IQ5 real-time PCR machine. After the PCR was complete, specificity of each primer pair was confirmed using melt curve analysis. Ct values were determined using PCR miner (Zhao and Fernald, 2005) and normalized to the reference ribosomal RNA, RPLP. Fold change in mRNA expression is relative to no stress control rats.

Western blot

Protein was extracted by homogenizing in RIPA buffer with protease inhibitor (1:100; Calbiochem, Billerica, MA) and phosphatase inhibitor (1:10; Roche, Basel, Switzerland). Following 30 min incubation on ice, samples were centrifuged at 12,000×g for 30 min at 4°C. The extracted protein was stored at −80°C. Total protein content was assessed using a BCA kit (Pierce, Waltham, MA). Samples were diluted 1:1 in laemmli buffer (Biorad) + 5% β-mercaptoethanol (Fisher, Waltham, MA) and run on 4–20% Mini-PROTEAN TGX gels (Biorad) at 100 V for 1.5 hr in 1× Tris-glycine-SDS buffer. They were then transferred to nitrocellulose membrane (Biorad) at 100 V for 1 hr in 1× Tris-glycine-SDS buffer with 20% methanol. Membranes were blocked for 1 hr with 5% milk in 0.1 M Tris buffered saline with 1% Tween-20 (Fisher)(TBS-t). Membranes were incubated in primary (rabbit anti-FGF2, 1:100; Abcam; mouse anti-actin, 1:10,000; Roche; rabbit anti-bdnf, 1:500; Abcam) in blocking solution overnight at 4°C. The next day, membranes were rinsed three times with TBS-t then incubated in secondary (LiCor [Lincoln, NE] IRDye 680LT Donkey anti-mouse or LiCor IRDye 800CW Donkey anti-rabbit, 1:20,000) for 1 hr. After three final rinses, membranes were visualized using a LiCor Odyssey scanner. The correct band size was found relative to a LiCor IRDye (680/800) protein marker ladder. All bands were quantified using LiCor Odyssey software, corrected for background and expressed relative to their corresponding actin band. Fold change in protein expression was then calculated relative to no stress control.

Plasma corticosterone sampling

All blood samples were centrifuged at 2000×g for 15 min and plasma was extracted and stored at −20°C until assayed. Corticosterone was measured using a Corticosterone EIA kit (Enzo Life Sciences, Farmingdale, NY).

Culturing of hippocampal NPCs

Isolation of neural stem/progenitor cells from adult rodents are described in detail in (Gage, 2000). Progenitors used in these experiments were purchased from Millipore (Billerica, MA; SCR022). Cells were cultured under standard conditions (37°C, 5% CO2) on poly-ornithine (Sigma) and laminin (Invitrogen) coated plates in N2-supplemented (Invitrogen) Dulbecco's modified Eagle medium (DMEM)/F-12 (1:1) (Invitrogen) with 20 ng/ml recombinant human FGF-2 (PeproTech, Rocky Hill, NJ).

Culturing of primary hippocampal astrocytes

Primary astrocyte cultures were prepared from P1–2 day old Sprague Dawley rat pup hippocampi using the method described by McCarthy and Vellis (McCarthy and de Vellis, 1980). Briefly, hippocampi were dissected in ice-cold media, chopped and digested using papain from papaya latex extract (Sigma) in HBSS (Invitrogen) for 20 min at 37°C. Papain was inactivated using 10% horse serum, cells were centrifuged for 1 min at 350×g and resuspended in HBSS and triturated by passing through serological and flame-polished pipettes of progressively smaller bores. Cells were then plated in DMEM (Invitrogen) supplemented with 10% fetal bovine serum (Axenia BioLogix, Dixon, CA) and 1% Penicillin/Streptomycin (Invitrogen) at a density of 3 × 106 in T75 flasks. After reaching confluency, flasks were shaken on an orbital shaker at 225 rpm for 2 hr at 37°C. Cells were then washed 5× with warm PBS to remove suspended microglia. Astrocytes were then trypsinized and re-plated in 100 mm dishes. 24 hr after plating, astrocytes were treated with 1 µM CORT or equivalent volume of EtOH vehicle for 3 hr. ACM was then collected, filtered with a 0.2 µm sterile filter and stored at −20°C.

Cell treatment for BrdU-labeling

In all studies, NPCs were FGF2 deprived for 24 hr then treated for 3 hr with the appropriate media. They were then pulsed with 30 µM BrdU and fixed 2 hr later with 4% paraformaldehyde for 10 min. Cell treatments were: 0 ng/ml FGF2 (+EtOH, n = 6; +CORT, n = 5), 20 ng/ml FGF2 (+EtOH, n = 5; +CORT, n = 5), CoC media (+EtOH, n = 6; +CORT, n = 6), ACM (+EtOH, n = 6; +CORT, n = 6). Treatments for the rat recombinant FGF2 experiment were 0 pg/ml FGF2 (n = 8) and 4 pg/ml FGF2 (n = 7).

Immunocytochemical BrdU staining and quantification

Fixed cells were rinsed with 0.1 M PBS, denatured in 1 N HCl at 37°C, rinsed and blocked in 5% normal donkey serum, 0.3% triton-100 in PBS. Cells were then incubated overnight at 4°C in mouse anti-brdu (1:500; BD Biosciences, Franklin Lakes, NJ) in 2% normal donkey serum in PBS. Cells were then rinsed and incubated in Cy3 anti-mouse (1:500; Jackson Immunoresearch) in 2% normal donkey serum in PBS, rinsed, counterstained with DAPI (1:20,000 in PBS) and coverslipped with DABCO anti-fading medium. BrdU+ and DAPI+ cells were counted in randomly sampled sites within each well using StereoInvestigator software (Microbrightfield) and a 20× air objective (Zeiss).

FGF2 neutralization

CoC media or ACM from primary astrocytes treated with EtOH or CORT was incubated with neutralizing FGF2 antibody (Millipore) for 1 hr at 37°C prior to use on NPCs. For nAb dose testing, NPCs were treated with 0 ng/ml FGF2 (0 µg/ml nAb, n = 8; 10 µg/ml nAb, n = 5, 20 µg/ml nAb, n = 3) or 20 ng/ml FGF2 (0 µg/ml nAb, n = 8; 10 µg/ml nAb, n = 7, 20 µg/ml nAb, n = 8). For ACM treatment with the FGF2 nAb, all n = 6.

FGF2 ELISA for ACM

For nAb specificity tests, the recommended standard curves of FGF1 and FGF2 from their respective rat-specific ELISAs (Antibodies Online) were preincubated in 0, 10 or 20 μg/ml nAb at 37°C for 1 hr before being quantified in the ELISA according to manufacturer's instructions. ACM from CORT (n = 3) and EtOH (n = 3) treated astrocytes was analyzed using the same rat FGF2 ELISA (Antibodies Online, Atlanta, GA).

Fear conditioning

Rats were immobilized for 3 hr or left undisturbed, then injected with 200 mg/kg BrdU. Either 2 days or 2 weeks after immobilization, rats were trained in contextual fear conditioning as per (Kirby et al., 2012b; 2 days: n = 10 con, n = 10 immob; 2 weeks: n = 20 con, n = 18 immob). The next day, rats were exposed to the fear conditioning context five times for 10 min each time with no shock (extinction). 24 hr later, they were re-exposed to the conditioning chamber for 10 min with no shock (extinction probe). A subset of rats (2 days: n = 10 con, n = 10 immob; 2 weeks: n = 10 con, n = 8 immob) was tested on an elevated plus maze 1 day prior to contextual fear conditioning (data not shown).

Statistics

In most studies, data were analyzed using a one-way ANOVA followed by Dunnett's posthoc tests with the appropriate control group as reference. For in vivo CORT injection studies with 30 min and 3 hr time points, data were analyzed using two-way ANOVA followed by Dunnett's posthoc test (0 mg/kg-30 min used as reference). For cell culture studies, data were analyzed using two-way ANOVA followed by Dunnett's posthoc tests. For BLA lesion studies, a repeated measures two-way ANOVA was used with hemisphere being the paired variable. When only two groups were being compared, unpaired t-tests were used in all cases. p≤0.05 was considered significant.

Acknowledgements

EDK was supported by a CIRM predoctoral fellowship and DoD NDSEG fellowship; LAB was supported by NSF GRFP.

Funding Statement

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Funding Information

This paper was supported by the following grants:

  • National Institute of Mental Health, Biobehavioral Research Awards for Innovative New Scientists (NIMH BRAINS) R01 MH087495 to Daniela Kaufer.
  • National Alliance for Research on Schizophrenia and Depression (NARSAD) Young Investigator Award R01 NS066005 to Daniela Kaufer.

Additional information

Competing interests

The authors declare that no competing interests exist.

Author contributions

EDK, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article.

SEM, Acquisition of data, Analysis and interpretation of data.

WGS, Conception and design, Acquisition of data, Analysis and interpretation of data.

DC, Conception and design, Acquisition of data, Drafting or revising the article.

MJL, Acquisition of data, Drafting or revising the article.

LAB, Acquisition of data, Drafting or revising the article.

DK, Conception and design, Drafting or revising the article.

Ethics

Animal experimentation: All animal procedures were approved by the UC Berkeley Animal Care and Use Committees and performed in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All surgery was performed under sodium pentobarbital anesthesia with appropriate pre and post-operative analgesia, and every effort was made to minimize suffering.

References

  • Abrous DN, Koehl M, Le Moal M. 2005. Adult neurogenesis: from precursors to network and physiology. Physiol Rev 85:523–69doi: doi: 10.1152/physrev.00055.2003. [PubMed] [Cross Ref]
  • Aimone JB, Deng W, Gage FH. 2010. Adult neurogenesis: integrating theories and separating functions. Trends Cogn Sci 14:325–37doi: doi: 10.1016/j.tics.2010.04.003. [PMC free article] [PubMed] [Cross Ref]
  • Allen NJ, et al. 2012. Astrocyte glypicans 4 and 6 promote formation of excitatory synapses via GluA1 AMPA receptors. Nature 486:410–4doi: doi: 10.1038/nature11059. [PMC free article] [PubMed] [Cross Ref]
  • Bhatnagar M, Cintra A, Chadi G, Lindberg J, Oitzl M, De Kloet ER, et al. 1997. Neurochemical changes in the hippocampus of the brown norway rat during aging. Neurobiol Aging 18:319–27doi: doi: 10.1016/S0197-4580(97)80314-4. [PubMed] [Cross Ref]
  • Buwalda B, Scholte J, De Boer SF, Coppens CM, Koolhaas JM. 2012. The acute glucocorticoid stress response does not differentiate between rewarding and aversive social stimuli in rats. Horm Behav 61:218–26doi: doi: 10.1016/j.yhbeh.2011.12.012. [PubMed] [Cross Ref]
  • Cameron HA, Gould E. 1994. Adult neurogenesis is regulated by adrenal steroids in the dentate gyrus. Neuroscience 61:203–9doi: doi: 10.1016/0306-4522(94)90224-0. [PubMed] [Cross Ref]
  • Chipperfield H, Bedi KS, Cool SM, Nurcombe V. 2002. Heparan sulfates isolated from adult neural progenitor cells can direct phenotypic maturation. Int J Dev Biol 46:661–70. [PubMed]
  • Conrad CD, Lupien SJ, McEwen BS. 1999. Support for a bimodal role for type II adrenal steroid receptors in spatial memory. Neurobiol Learn Mem 72:39–46doi: doi: 10.1006/nlme.1998.3898. [PubMed] [Cross Ref]
  • Dagyte G, Van der Zee EA, Postema F, Luiten PG, Den Boer JA, Trentani A, et al. 2009. Chronic but not acute foot-shock stress leads to temporary suppression of cell proliferation in rat hippocampus. Neuroscience 162:904–13doi: doi: 10.1016/j.neuroscience.2009.05.053. [PubMed] [Cross Ref]
  • De Kloet ER, Vreugdenhil E, Oitzl MS, Joels M. 1998. Brain corticosteroid receptor balance in health and disease. Endocr Rev 19:269–301doi: doi: 10.1210/er.19.3.269. [PubMed] [Cross Ref]
  • Deng W, Saxe MD, Gallina IS, Gage FH. 2009. Adult-born hippocampal dentate granule cells undergoing maturation modulate learning and memory in the brain. J Neurosci 29:13532–42doi: doi: 10.1523/JNEUROSCI.3362-09.2009. [PMC free article] [PubMed] [Cross Ref]
  • Eroglu C, Barres BA. 2010. Regulation of synaptic connectivity by glia. Nature 468:223–31doi: doi: 10.1038/nature09612. [PubMed] [Cross Ref]
  • Fanselow MS, Dong H-W. 2010. Are the dorsal and ventral hippocampus functionally distinct structures. Neuron 65:7–19doi: doi: 10.1016/j.neuron.2009.11.031. [PMC free article] [PubMed] [Cross Ref]
  • Forget C, Stewart J, Trudeau L-E. 2006. Impact of basic FGF expression in astrocytes on dopamine neuron synaptic function and development. Eur J Neurosci 23:603–16doi: doi: 10.1111/j.1460-9568.2006.04570.x. [PubMed] [Cross Ref]
  • Gage FH. 2000. Mammalian neural stem cells. Science 287:1433–8doi: doi: 10.1126/science.287.5457.1433. [PubMed] [Cross Ref]
  • Gould E, McEwen BS, Tanapat P, Galea LA, Fuchs E. 1997. Neurogenesis in the dentate gyrus of the adult tree shrew is regulated by psychosocial stress and NMDA receptor activation. J Neurosci 17:2492–8. [PubMed]
  • Gubba EM, Fawcett JW, Herbert J. 2004. The effects of corticosterone and dehydroepiandrosterone on neurotrophic factor mRNA expression in primary hippocampal and astrocyte cultures. Brain Res Mol Brain Res 127:48–59doi: doi: 10.1016/j.molbrainres.2004.05.004. [PubMed] [Cross Ref]
  • Hanson ND, Owens MJ, Boss-Williams KA, Weiss JM, Nemeroff CB. 2011. Several stressors fail to reduce adult hippocampal neurogenesis. Psychoneuroendocrinology 36:1520–9doi: doi: 10.1016/j.psyneuen.2011.04.006. [PMC free article] [PubMed] [Cross Ref]
  • Katz M, Liu C, Schaer M, Parker KJ, Ottet MC, Epps A, et al. 2009. Prefrontal plasticity and stress inoculation-induced resilience. Dev Neurosci 31:293–9doi: doi: 10.1159/000216540. [PMC free article] [PubMed] [Cross Ref]
  • Kee N, Teixeira CM, Wang AH, Frankland PW. 2007. Preferential incorporation of adult-generated granule cells into spatial memory networks in the dentate gyrus. Nat Neurosci 10:355–62doi: doi: 10.1038/nn1847. [PubMed] [Cross Ref]
  • Kirby ED, Jensen K, Goosens KA, Kaufer D. 2012a. Stereotaxic surgery for excitotoxic lesion of specific brain areas in the adult rat. J Vis Exp 65:4079doi: doi: 10.3791/4079. [PubMed] [Cross Ref]
  • Kirby ED, Kaufer D. 2009. Stress and adult neurogenesis in the mammalian central nervous system. In: Soreq H, Friedman A, Kaufer D, editors. , editors. STRESS: from molecules to behavior. Weinheim: Wiley-Blackwell; p. 71–91.
  • Kirby ED, Friedman AR, Covarrubias D, Ying C, Sun WG, Goosens KA, et al. 2012b. Basolateral amygdala regulation of adult hippocampal neurogenesis and fear-related activation of newborn neurons. Mol Psychiatry 17:527–36doi: doi: 10.1038/mp.2011.71. [PubMed] [Cross Ref]
  • Kitamura T, Saitoh Y, Takashima N, Murayama A, Niibori Y, Ageta H, et al. 2009. Adult neurogenesis modulates the hippocampus-dependent period of associative fear memory. Cell 139:814–27doi: doi: 10.1016/j.cell.2009.10.020. [PubMed] [Cross Ref]
  • Knox D, Nault T, Henderson C, Liberzon I. 2012a. Glucocorticoid receptors and extinction retention deficits in the single prolonged stress model. Neuroscience 223:163–73doi: doi: 10.1016/j.neuroscience.2012.07.047. [PubMed] [Cross Ref]
  • Knox D, George SA, Fitzpatrick CJ, Rabinak CA, Maren S, Liberzon I. 2012b. Single prolonged stress disrupts retention of extinguished fear in rats. Learn Mem 19:43–9doi: doi: 10.1101/lm.024356.111. [PubMed] [Cross Ref]
  • Korosi A, Baram TZ. 2010. Plasticity of the stress response early in life: mechanisms and significance. Dev Psychobiol 52:661–70doi: doi: 10.1002/dev.20490. [PMC free article] [PubMed] [Cross Ref]
  • Korz V, Frey JU. 2005. Bidirectional modulation of hippocampal long-term potentiation under stress and no-stress conditions in basolateral amygdala-lesioned and intact rats. J Neurosci 25:7393–400doi: doi: 10.1523/JNEUROSCI.0910-05.2005. [PubMed] [Cross Ref]
  • Kronenberg G, Bick-Sander A, Bunk E, Wolf C, Ehninger D, Kempermann G. 2006. Physical exercise prevents age-related decline in precursor cell activity in the mouse dentate gyrus. Neurobiol Aging 27:1505–13doi: doi: 10.1016/j.neurobiolaging.2005.09.016. [PubMed] [Cross Ref]
  • Kucukdereli H, Allen NJ, Lee AT, Feng A, Ozlu MI, Conatser LM, et al. 2011. Control of excitatory CNS synaptogenesis by astrocyte-secreted proteins Hevin and SPARC. Proc Natl Acad Sci USA 108:E440–9doi: doi: 10.1073/pnas.1104977108. [PubMed] [Cross Ref]
  • Leuner B, Glasper ER, Gould E. 2010. Sexual experience promotes adult neurogenesis in the hippocampus despite an initial elevation in stress hormones. PLoS One 5:e11597doi: doi: 10.1371/journal.pone.0011597. [PMC free article] [PubMed] [Cross Ref]
  • Luksys G, Sandi C. 2011. Neural mechanisms and computations underlying stress effects on learning and memory. Curr Opin Neurobiol 21:502–8doi: doi: 10.1016/j.conb.2011.03.003. [PubMed] [Cross Ref]
  • Lupien SJ, McEwen BS. 1997. The acute effects of corticosteroids on cognition: integration of animal and human model studies. Brain Res Brain Res Rev 24:1–27doi: doi: 10.1016/S0165-0173(97)00004-0. [PubMed] [Cross Ref]
  • Lyons DM, Parker KJ. 2007. Stress inoculation-induced indications of resilience in monkeys. J Trauma Stress 20:423–33doi: doi: 10.1002/jts.20265. [PubMed] [Cross Ref]
  • Lyons DM, Buckmaster PS, Lee AG, Wu C, Mitra R, Duffey LM, et al. 2010. Stress coping stimulates hippocampal neurogenesis in adult monkeys. Proc Natl Acad Sci USA 107:14823–7doi: doi: 10.1073/pnas.0914568107. [PubMed] [Cross Ref]
  • Ma DK, Jang MH, Guo JU, Kitabatake Y, Chang ML, Pow-Anpongkul N, et al. 2009. Neuronal activity-induced Gadd45b promotes epigenetic DNA demethylation and adult neurogenesis. Science 323:1074–7doi: doi: 10.1126/science.1166859. [PMC free article] [PubMed] [Cross Ref]
  • Marín-Burgin A, Mongiat LA, Pardi MB, Schinder AF. 2012. Unique processing during a period of high excitation/inhibition balance in adult-born neurons. Science 335:1238–42doi: doi: 10.1126/science.1214956. [PMC free article] [PubMed] [Cross Ref]
  • McCarthy KD, de Vellis J. 1980. Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J Cell Biol 85:890–902doi: doi: 10.1083/jcb.85.3.890. [PMC free article] [PubMed] [Cross Ref]
  • McEwen BS. 2004. Protection and damage from acute and chronic stress: allostasis and allostatic overload and relevance to the pathophysiology of psychiatric disorders. Ann N Y Acad Sci 1032:1–7doi: doi: 10.1196/annals.1314.001. [PubMed] [Cross Ref]
  • McGaugh JL. 2004. The amygdala modulates the consolidation of memories of emotionally arousing experiences. Ann Rev Neurosci 27:1–28doi: doi: 10.1016/j.nlm.2008.10.010. [PubMed] [Cross Ref]
  • Mirescu C, Gould E. 2006. Stress and adult neurogenesis. Hippocampus 16:233–8doi: doi: 10.1002/hipo.20155. [PubMed] [Cross Ref]
  • Mocchetti I, Spiga G, Hayes VY, Isackson PJ, Colangelo AM. 1996. Glucocorticoids differentially increase nerve growth factor and basic fibroblast growth factor expression in the rat brain. J Neurosci 16:2141–8. [PubMed]
  • Molteni R, Fumagalli F, Magnaghi V, Roceri M, Gennarelli M, Racagni G, et al. 2001. Modulation of fibroblast growth factor-2 by stress and corticosteroids: from developmental events to adult brain plasticity. Brain Res Brain Res Rev 37:249–58doi: doi: 10.1016/S0165-0173(01)00128-X. [PubMed] [Cross Ref]
  • Parker KJ, Maestripiero D. 2011. Identifying key features of early stressful experiences that produce stress vulnerability and resilience in primates. Neurosci Biobehav Rev 35:1466–83doi: doi: 10.1016/j.neubiorev.2010.09.003. [PMC free article] [PubMed] [Cross Ref]
  • Quirarte GL, Roozendaal B, McGaugh JL. 1997. Glucococrticoid enhancement of memory storage involves noradrenergic activation in the basolateral amygdala. Proc Natl Acad Sci USA 94:14048–53doi: doi: 10.1073/pnas.94.25.14048. [PubMed] [Cross Ref]
  • Roozendaal B, McEwen BS, Chattarji S. 2009a. Stress, memory and the amygdala. Nat Rev Neurosci 10:423–33doi: doi: 10.1038/nrn2651. [PubMed] [Cross Ref]
  • Roozendaal B, Nguyen BT, Power AE, McGaugh JL. 1999. Basolateral amygdala noradrenergic influence enables enhancement of memory consolidation induced by hippocampal glucocorticoid receptor activation. Proc Natl Acad Sci USA 96:11642–7doi: doi: 10.1073/pnas.96.20.11642. [PubMed] [Cross Ref]
  • Roozendaal B, McReynolds JR, Van der Zee EA, Lee S, McGaugh JL, McIntyre CK. 2009b. Glucocorticoid effects on memory consolidation depend on functional interactions between the medial prefrontal cortex and basolateral amygdala. J Neurosci 29:14299–308doi: doi: 10.1523/JNEUROSCI.3626-09.2009. [PMC free article] [PubMed] [Cross Ref]
  • Sahay A, Scobie KN, Hill AS, O'Carroll CM, Kheirbek MA, Burghardt NS, et al. 2011. Increasing adult hippocampal neurogenesis is sufficient to improve pattern separation. Nature 472:466–70doi: doi: 10.1038/nature09817. [PMC free article] [PubMed] [Cross Ref]
  • Salehi B, Cordero MI, Sandi C. 2010. Learning under stress: the inverted-U-shape function revisited. Learn Mem 17:522–30doi: doi: 10.1101/lm.1914110. [PubMed] [Cross Ref]
  • Snyder JS, Choe JS, Clifford MA, Jeurling SI, Hurley P, Brown A, et al. 2009. Adult-born hippocampal neurons are more numerous, faster maturing, and more involved in behavior in rats than in mice. J Neurosci 29:14484–95doi: doi: 10.1523/JNEUROSCI.1768-09.2009. [PMC free article] [PubMed] [Cross Ref]
  • Song H, Stevens CF, Gage FH. 2002. Astroglia induce neurogenesis from adult neural stem cells. Nature 417:39–44doi: doi: 10.1038/417039a. [PubMed] [Cross Ref]
  • Stone SS, Teixeira CM, Zaslavsky K, Wheeler AL, Martinez-Canabal A, Wang AH, et al. 2010. Functional convergence of developmentally and adult-generated granule cells in dentate gyrus circuits supporting hippocampus-dependent memory. Hippocampus 21:1348–62doi: doi: 10.1002/hipo.20845. [PubMed] [Cross Ref]
  • Tanti A, Rainer Q, Minier F, Surget A, Belzung C. 2012. Differential environmental regulation along the septo-temporal axis of the hippocampus. Neuropharmacology 63:374–84doi: doi: 10.1016/j.neuropharm.2012.04.022. [PubMed] [Cross Ref]
  • Thomas RM, Hotsenpiller G, Peterson DA. 2007. Acute psychosocial stress reduces cell survial in adult hippcampal neurogenesis without altering proliferation. J Neurosci 27:2734–43doi: doi: 10.1523/JNEUROSCI.3849-06.2007. [PubMed] [Cross Ref]
  • Thomas RM, Urban JH, Peterson DA. 2006. Acute exposure to predator odor elicits a robust increase in corticosterone and a decrease in activity without altering proliferation in the adult rat hippocampus. Exp Neurol 201:308–15doi: doi: 10.1016/j.expneurol.2006.04.010. [PubMed] [Cross Ref]
  • Unemura K, Kume T, Kondo M, Maeda Y, Izumi Y, Akaike A. 2012. Glucocorticoids decrease astrocyte numbers by reducing glucocorticoid receptor expression in vitro and in vivo. J Pharmacol Sci 119:30–9doi: doi: 10.1254/jphs.12047FP. [PubMed] [Cross Ref]
  • Wolf SA, Steiner B, Wengner A, Lipp M, Kammertoens T, Kempermann G. 2009. Adaptive peripheral immune response increases proliferation of neural precursor cells in the adult hippocampus. FASEB J 23:3121–8doi: doi: 10.1096/fj.08-113944. [PubMed] [Cross Ref]
  • Wong EY, Herbert J. 2004. The corticoid environment: a determining factor for neural progenitors' survival in the adult hippocampus. Eur J Neurosci 20:2491–8doi: doi: 10.1111/j.1460-9568.2004.03717.x. [PMC free article] [PubMed] [Cross Ref]
  • Zhao S, Fernald RD. 2005. Comprehensive algorithm for quantitative real-time polymerase chain reaction. J Comput Biol 12:1045–62doi: doi: 10.1089/cmb.2005.12.1047. [PMC free article] [PubMed] [Cross Ref]
eLife. 2013; 2: e00362.
Published online 2013 April 16. doi:  10.7554/eLife.00362.014

Decision letter

Russ Fernald, Reviewing editor
Russ Fernald, Stanford University, United States;

eLife posts the editorial decision letter and author response on a selection of the published articles (subject to the approval of the authors). An edited version of the letter sent to the authors after peer review is shown, indicating the substantive concerns or comments; minor concerns are not usually shown. Reviewers have the opportunity to discuss the decision before the letter is sent (see review process). Similarly, the author response typically shows only responses to the major concerns raised by the reviewers.

Thank you for choosing to send your work entitled “Acute stress enhances adult hippocampal neurogenesis and memory via secreted astrocytic FGF2 in rats” for consideration at eLife. Your article has been evaluated by a Senior editor and 3 reviewers, one of whom is a member of our Board of Reviewing Editors.

The Reviewing editor and the other reviewers discussed their comments before we reached this decision, and the Reviewing editor has assembled the following comments to help you prepare a revised submission. The reviewers agree that this is a thorough and interesting study, but they have the following suggestions:

1) This study reveals a new domain of the inverse-U phenomenon in stress and glucocorticoid action. To make the paper more broadly appealing, this should be discussed in the Introduction.

2) The Introduction should also include comments about the BLA given its role in mediating interactions between the amygala and hippocampus.

3) To be convinced on the causal role of astrocytic FGF2, additional data are needed to demonstrate that the in vivo increase in FGF2 is from astrocytes. Since neonatal astrocyte cultures are often contaminated, these sources need to be excluded using, e.g., in situ hybridization or immunohistochemistry on hippocampus tissue of similarly aged animals in vivo.

4) A related concern is the certainty that no other members of the FGF family are involved. Is the FGF2 blocking antibody specific to that form?

5) In addition, can you show actual levels of FGF2 present rather than relative levels, and is any FGF2 released from untreated astrocyte cultures (an important question since untreated ACM has no effect on NPC proliferation)?

6) The fear extinction data are interesting, but they feel like they belong in a different paper.

eLife. 2013; 2: e00362.
Published online 2013 April 16. doi:  10.7554/eLife.00362.015

Author response

1) This study reveals a new domain of the inverse-U phenomenon in stress and glucocorticoid action. To make the paper more broadly appealing, this should be discussed in the Introduction.

We have added a paragraph introducing and discussing the inverted U function as the second paragraph of the Introduction. In addition, we have also discussed how this function potentially relates to previous literature on adult neurogenesis in the fifth paragraph and to our findings in the sixth paragraph.

2) The Introduction should also include comments about the BLA given its role in mediating interactions between the amygala and hippocampus.

Comments about the role of the BLA in influencing hippocampal stress responses and adult neurogenesis have been added to the third paragraph of the Introduction.

3) To be convinced on the causal role of astrocytic FGF2, additional data are needed to demonstrate that the in vivo increase in FGF2 is from astrocytes. Since neonatal astrocyte cultures are often contaminated, these sources need to be excluded using, for example, in situ hybridization or immunohistochemistry on hippocampus tissue of similarly aged animals in vivo.

We have added new data on FGF2 protein expression in GFAP+ astrocytes in adult male rats in a new Figure 7 (former Figure 7 is now Figure 8 and so on). We measured FGF2 expression in astrocytes using double immunohistochemical labeling for FGF2 protein and the astrocyte marker, glial fibrillary acidic protein (GFAP), in fixed hippocampal tissue sections from rats described in Figure 2 (con, immob, 0 mg/kg and 40 mg/kg CORT), all perfused 3 hr after immobilization or injection, as appropriate.

Consistent with previous reports [1], FGF2-immunoreactive cells were found throughout the DG and the hilus, with staining primarily in the cell body and nucleus. Z-stacks of the dentate gyrus and hilus were acquired using confocal microscopy, and used to identify GFAP+ vs GFAP- cells. The dentate gyrus is primarily composed of dentate granule neurons and while almost every cell expressed FGF2, we found very few GFAP+ cells within the DG, as expected. Given the ubiquitous and closely-packed FGF2 immunoreactivity in the DG, we had to measure mean optical density (with background correction) throughout the Z-stack of FGF2 expression in the DG (rather than in individual cells). No effect of stress or CORT injection on DG FGF2 expression was found using this measure.

In contrast, in the hilus, we could clearly identify and measure individual GFAP+ (and GFAP-) cells. Almost all GFAP+ cells were FGF2+. Given the mixed cell population but ubiquitous presence of FGF2 in GFAP+ cells, we measured integrated optical density of individual FGF2+ cells that were either GFAP+ or GFAP-. We found increased FGF2 signal in GFAP+ but not GFAP- cells in the hilus following both immobilization and 40 mg/kg CORT injection. These findings suggest that the FGF2 increase seen in whole hippocampal lysates by real time quantitative PCR and western blot (Figure 6) are most likely due to astrocytic FGF2 secretion. Moreover, while other cell types in other hippocampal subregions not quantified here could contribute to overall changes in FGF2, the DG and hilus are the regions flanking the neurogenic niche in the subgranular zone, and are therefore the most likely sources of growth factor changes that influence neurogenesis. These data are presented in the Results section following the section describing Figure 6. The relevant methods have also been added to the Materials and methods section where appropriate.

4) A related concern is the certainty that no other members of the FGF family are involved. Is the FGF2 blocking antibody specific to that form?

Of all the 23 members of the FGF family, FGF2 is the most potent growth factor for NPCs [2]. It is also one of the few FGFs known to be predominantly secreted by astrocytes (as opposed to neurons) [2] in the adult CNS. However, FGF1 has been reported to both stimulate NPCs [3] and be secreted from adult CNS astrocytes [4]. Previous work using the FGF2 nAb that we used in the present studies confirm that the nAb does not cross react with bovine or human FGF1 [5–7]. To confirm that the nAb does not neutralize rat FGF1, we performed ELISAs with either rat FGF2 or rat FGF1 and compared standard curves of protein dilutions with or without preincubation with the nAb. We found that while FGF2 was significantly neutralized at all concentrations tested, FGF1 availability in the assay was not significantly affected by the nAb. These data are now presented in Figure 9B,C. Given these specificity data and the prominent, nearly exclusive role of FGF2 in adult NPC proliferation, we feel relatively confident that the effects of this FGF2 nAb are specific to FGF2 neutralization in these studies.

5) In addition, can you show actual levels of FGF2 present rather than relative levels, and is any FGF2 released from untreated astrocyte cultures (an important question since untreated ACM has no effect on NPC proliferation)?

The FGF2 levels are now expressed in pg/ml from a rat FGF2 ELISA. Consistent with previous work, when astrocytes were only exposed to vehicle, FGF2 levels were similar to blank, suggesting very low basal secretion of FGF2 [8]. However, when treated with 1 μM CORT, FGF2 was found in the media at 3.5 pg/ml on average (Figure 9A). To confirm that FGF2 at concentrations in this range were sufficient to stimulate NPC proliferation, we treated adult rat NPCs with 0 and 4 pg/ml rat recombinant FGF2 for 3h, pulsed with BrdU and then fixed the cells 2h later (design in Figure 5A). We found that as little as 4 pg/ml rat rFGF2 increased the percent of BrdU+ NPCs (reported in the text of the results section for Figure 9). These data demonstrate that the levels of FGF2 found in ACM after CORT stimulation are sufficient to enhance NPC proliferation.

6) The fear extinction data are interesting, but they feel like they belong in a different paper.

Though the fear extinction data do represent a departure from the mechanistic data focusing on time points close to the stressor, we feel that including these data, and in particular the new neuron activation findings, are important to the behavioral relevance of the rest of the manuscript. Adult NPCs require 2–4 weeks to mature and become physiologically active in the adult rat [9]. Therefore, the relevance of an enhancement in proliferation immediately after stress is questionable without some longer-term effects. Since we do not see more new neurons persisting in immobilized rats two weeks after the stressor (Figure 10G,H), it might seem that the effect of acute stress does not have any functional output.

However, our finding that the neurons that have survived over the two weeks activate more in response to fear extinction in immob than control rats, and that these rats have a coincident behavioral memory benefit that does not appear at earlier time points, strongly suggests that the enhanced neurogenic potential of the hippocampus, following acute stress, has important behavioral relevance. Including behavioral and neuron activation data such as this is a common practice in the adult neurogenesis literature [10–12]. We therefore request to keep these data in the manuscript as we feel many researchers in the adult neurogenesis field will find them helpful in interpreting our data and integrating it into their understanding of how both stress and adult neurogenesis relate to memory and hippocampal function. We have added some additional explanation to the Figure 10 results sections to emphasize these points.

References

1. Bhatnagar, M et al. Neurochemical changes in the hippocampus of the brown norway rat during aging. Neurobiol Aging 18, 319–27 (1997)

2. Reuss, B. & von Bohlen un Halbach, O. Fibroblast growth factors and their receptors in the central nervous system. Cell and Tissue Research 313, 139–57 (2003) 10.1007/s00441-003-0756-7

3. Cheng, X et al. Acidic fibroblast growth factor delivered intranasally induces neurogenesis and angiogenesis in rats after ischemic stroke. Neurological Research 33, 675–80 (2011) 10.1179/1743132810Y.0000000004

4. Lee, M et al. Acidic fibroblast growth factor (FGF) potentiates glial-mediated neurotoxicity by activating FGFR2 IIIb protein. Journal of Biological Chemistry 286, 41230–45 (2011) 10.1074/jbc.M111.270470

5. Wang, L et al. A novel monoclonal antibody to fibroblast growth factor 2 effectively inhibits growth of hepatocellular carcinoma xenografts. Molecular Cancer Therapy 11, 864‐872 (2012) 10.1158/1535-7163.MCT-11-0813

6. Amano, O, Yoshitake, Y, Nishikawa, K & Iseki, S. Immunocytochemical localization of basic fibroblast growth factor in the rat pituitary gland. Archives of Histology and Cytology 56, 269–76 (1993)

7. Matsuzaki, K, Yoshitake, Y, Matuo, Y, Sasaki, H & Nishikawa, K. Monoclonal antibodies against heparin-binding growth factor II/basic fibroblast growth factor that block its biological activity: Invalidity of the antibodies for tumor angiogenesis. Proc Natl Acad Sci USA 86, 9911–5 (1989)

8. Forget, C, Stewart, J & Trudeau, L-E. Impact of basic FGF expression in astrocytes on dopamine neuron synaptic function and development. Eur J Neurosci 23, 603–16 (2006) 10.1111/j.1460-9568.2006.04570.x

9. Snyder, JS et al. Adult-born hippocampal neurons are more numerous, faster maturing, and more involved in behavior in rats than in mice. J Neurosci 29, 14484–95 (2009) 10.1523/JNEUROSCI.1768-09.2009

10. Epp, JR, Haack, AK & Galea, LA. Activation and survival of immature neurons in the dentate gyrus with spatial memory is dependent on time of exposure to spatial learning and age of cells at examination. Neurobiology of Learning and Memory 95, 316–25 (2011) 10.1016/j.nlm.2011.01.001

11. Farioli-Vecchioli, S et al. The timing of differentiation of adult hippocampal neurons is crucial for spatial memory. PLoS Biology 6, 2188–204 (2008) 10.1371/journal.pbio.0060246

12. Kee, N, Teixeira, CM, Wang, AH & Frankland, PW. Preferential incorporation of adult-generated granule cells into spatial memory networks in the dentate gyrus. Nat Neurosci 10, 355–62 (2007) 10.1038/nn1847


Articles from eLife are provided here courtesy of eLife Sciences Publications, Ltd