|Home | About | Journals | Submit | Contact Us | Français|
The term “mitochondrial permeability transition” (MPT) refers to an abrupt increase in the permeability of the inner mitochondrial membrane to low molecular weight solutes. Due to osmotic forces, MPT is paralleled by a massive influx of water into the mitochondrial matrix, eventually leading to the structural collapse of the organelle. Thus, MPT can initiate mitochondrial outer membrane permeabilization (MOMP), promoting the activation of the apoptotic caspase cascade as well as of caspase-independent cell death mechanisms. MPT appears to be mediated by the opening of the so-called “permeability transition pore complex” (PTPC), a poorly characterized and versatile supramolecular entity assembled at the junctions between the inner and outer mitochondrial membranes. In spite of considerable experimental efforts, the precise molecular composition of the PTPC remains obscure and only one of its constituents, cyclophilin D (CYPD), has been ascribed with a crucial role in the regulation of cell death. Conversely, the results of genetic experiments indicate that other major components of the PTPC, such as voltage-dependent anion channel (VDAC) and adenine nucleotide translocase (ANT), are dispensable for MPT-driven MOMP. Here, we demonstrate that the c subunit of the FO ATP synthase is required for MPT, mitochondrial fragmentation and cell death as induced by cytosolic calcium overload and oxidative stress in both glycolytic and respiratory cell models. Our results strongly suggest that, similar to CYPD, the c subunit of the FO ATP synthase constitutes a critical component of the PTPC.
Mitochondrial outer membrane permeabilization (MOMP) is widely considered as a point-of-no-return in the cascade of events whereby lethal signals that originate in the intracellular microenvironment are translated into apoptotic cell death.1-3 In addition, MOMP is involved in some instances of apoptosis as elicited by extracellular signals of distress as well as in non-apoptotic forms of cell death, including regulated necrosis.4,5 Irrespective of the initiating mechanism, MOMP results in: (1) the dissipation of the mitochondrial transmembrane potential (ΔΨm), in turn engendering a complete arrest of mitochondrial ATP synthesis and other ΔΨm-dependent activities (e.g., protein import) and (2) the release (into the cytosol) of cytotoxic proteins that normally serve physiological functions in the mitochondrial intermembrane space, such as cytochrome c.6-8 Thus, when MOMP affects the vast majority of a cell’s mitochondria both caspase-dependent and -independent mechanisms are activated to ultimately seal the cell’s fate.9 Owing to its central role in cell death regulation, MOMP is involved in the pathophysiology of human diseases as diverse as cancer, ischemia and viral infections.10,11
MOMP can either be initiated at the outer mitochondrial membrane, owing to the pore-forming activity of the pro-apoptotic BCL-2 family members BAX and BAK,12 or ensue the so-called “mitochondrial permeability transition” (MPT), an abrupt increase in the permeability of the inner mitochondrial membrane to low molecular weight solutes.9,13-15 Due to osmotic forces, MPT results in a massive influx of water into the mitochondrial matrix, eventually leading to the structural breakdown of the organelle.9,13 MPT appears to be mediated by the so-called “permeability transition pore complex” (PTPC), a multiprotein entity lined up at the junctions between the inner and outer mitochondrial membranes.9,13 During the last couple of decades, two models have been put forward to mechanistically link MPT with the observation that the PTPC can assume two distinct states of conductance.16 On one hand, it has been proposed that one of the main PTPC components, i.e., voltage-dependent anion channel (VDAC), would normally exist in an “open” state and would promote MPT upon “closure.”17,18 On the other hand, a low-conductance conformation of the PTPC has been suggested to allow for the physiological exchange of metabolites between the mitochondrial matrix and the cytosol. According to this model, the PTPC would mediate MPT following a conformational shift to a high-conductance state.19,20 To date, there is no model that would reconcile these two mutually exclusive working hypotheses.
The precise molecular composition of the PTPC has been subject of intense investigation yet remains poorly understood.9 Indeed, whereas a few proteins have been indicated as core components of the PTPC, including voltage-dependent anion channel (VDAC), adenine nucleotide translocase (ANT) and cyclophilin D (CYPD), genetic experiments have confirmed a critical contribution to MPT in vivo only for the latter.21-23 Conversely, neither VDAC nor any of the main isoforms of ANT appear to be required for the lethal functions of the PTPC.24-26 Along similar lines, dozens of cytosolic (e.g., hexokinase II) and mitochondrial (e.g., translocator protein of 18 KDa) interactors of the PTPC have been described, suggesting that the PTPC constitutes a highly dynamic signaling entity,27 yet none of these proteins seems to be required for MPT-driven MOMP. Of note, both pro- and anti-apoptotic BCL-2 family members, including BAX, BID, BCL-2 and BCL-XL,18,20,28 have been shown to physically bind to—and hence modulate the function of—PTPC components, indicating that the molecular machineries mediating MPT-driven and primary MOMP do not operate in a mutually exclusive manner.
The mitochondrial F1FO ATP synthase has previously been shown to interact with ANT and the mitochondrial phosphate carrier (SLC25A3), both of which are involved in MPT,29,30 to form multiprotein complexes that catalyze the final steps of mitochondrial ATP synthesis also known as “ATP synthasomes.”31 In addition, CYPD as well as two distinct anti-apoptotic members of the BCL-2 family, namely BCL-XL and MCL-1, have recently been reported to regulate mitochondrial ATP synthesis by physically interacting with the F1FO ATP synthase.32-35 Driven by these observations and by the facts that: (1) a selective inhibitor of the FO subunit of ATP synthase, i.e., oligomycin, is able to prevent cell death as induced by tumor necrosis factor α (TNFα),36 the multi-kinase inhibitor staurosporine37 or BAX overexpression;38 and that (2) the activity of both the PTPC and F1FO ATP synthase is modulated by Mg2+ ions;39,40 we decided to investigate the role of FO ATP synthase subunits in MPT.
Here, we reveal the important finding that the c subunit of the FO ATP synthase is required for MPT-driven mitochondrial fragmentation and cell death triggered by cytosolic calcium overload and oxidative stress.
The membrane-spanning component of human mitochondrial ATP synthase (best known as FO, reflecting the fact that it can be inhibited by oligomycin) is composed by no less than nine polypeptides (a, b, c, d, e, f, g, F6 and 8), while the soluble catalytic core of the complex (F1) consists of five different subunits (α, β, γ, δ and ε).41,42 Among the nine polypeptides building up FO, a, b and c display an elevated degree of evolutionary conservation (subunits d-g, F6 and 8 are found in mitochondrial, but not prokaryotic, ATP synthases) and are sufficient for the complex to translocate protons across lipid bilayers.43,44 While the a subunit is encoded by mitochondrial DNA, b and c are encoded by the nuclear genome.45
It has previously been shown that ρ0 cells (which lack mitochondrial DNA) are equipped with a functional PTPC, de facto excluding a prominent role for the a subunit of the FO synthase in MPT.46,47 Moreover, (1) the c, but not the b, subunit has been ascribed with conductive properties;48 and (2) a peptide displaying a consistent degree of similarity to the c subunit has been indicated as a putative regulator of the PTPC.49 Therefore, we focused on the putative contribution of the c subunit of the FO ATP synthase to the lethal functions of the PTPC.
To understand whether and how the c subunit participates in MPT, we decided to genetically manipulate its expression levels in a cell model that heavily rely on glycolysis (rather than on mitochondria) for ATP synthesis, i.e., human cervical carcinoma HeLa cells.50 This choice stemmed from the fact that the activity of the PTPC is modulated by various intermediate metabolites, including NADH, ATP, ADP and inorganic phosphate.9 As the c subunit is coded by three distinct genes (ATP5G1, ATP5G2 and ATP5G3) that generate polypeptides bearing different mitochondrial import sequences but forming the very same mature protein,45 we had to envision a depletion strategy based on a mix of three commercial validated small-interfering RNAs (siRNAs) (Fig. 1A). In addition, we generated a construct for the overexpression of MYC-tagged ATP5G1 under the control of the cytomegalovirus (CMV) immediate early promoter. Upon transfection, the exogenous c subunit properly co-localized with the mitochondrial marker heat shock 60 kDa protein 1 (HSPD1, best known as HSP60), as determined by two-color 3D deconvolution immunofluorescence microscopy (Fig. 1B). As a control, we used a commercial non-targeting siRNA as well as a validated siRNA specific for the α polypeptide of the F1 subunit (ATP5A1) (Fig. S1A).
Histamine promotes the transfer of Ca2+ from reticular stores to mitochondria, resulting in increased mitochondrial ATP synthesis.51,52 The administration of 100 μM histamine to HeLa cells, however, had no effect on mitochondrial ATP levels (assessed with a mitochondrially targeted firefly luciferase) (Fig. 1C). In addition, this response was not influenced by the depletion of the FO c subunit (Fig. 1C) or the F1 α subunit (Fig. S1B), confirming the near-to-purely glycolytic nature of this cellular model. Conversely, HeLa cells overexpressing the c subunit responded to histamine with a comparatively higher increase in mitochondrial ATP synthesis (Fig. 1D). Such a metabolic effect was paralleled by a reduction in Δψm, as monitored in cellula by means of a Δψm-sensitive fluorochrome (i.e., tetramethylrhodamine methyl ester, TMRM) (Fig. 1E). Moreover, the transfection-enforced overexpression of the FO c subunit promoted mitochondrial fragmentation, as assessed by the virtual reconstruction of the mitochondrial network and automated image analysis in cells co-expressing a mitochondrially targeted green fluorescent protein variant (mtGFP) (Fig. 1F). Of note, the increased number of mtGFP+ objects observed in HeLa cells overexpressing the FO c subunit did not stem from a net increase in mitochondrial mass, as the protein levels of the FO a subunit, the F1 α subunit, cytochrome c and VDAC1 were not altered in this setting (Fig. 1A). Moreover, neither Δψm nor the integrity of the mitochondrial network was significantly affected by the depletion of the FO c subunit (Fig. 1E and F) or that of the F1 α subunit (Fig. S1C).
Altogether, these results suggest that, in a near-to-purely glycolytic cell model, the genetic inhibition of F1FO ATP synthase subunits do not affect mitochondrial ATP synthesis.
To monitor the opening of the PTPC in living cells, we took advantage of the calcein-Co2+ assay.53,54 In this setting, cells are loaded with an esterified form of calcein, which is retained within the plasma membrane (thanks to the activity of cytoplasmic esterases) and freely diffuses into all subcellular compartments (including mitochondria), followed by bivalent cobalt (Co2+) ions. Co2+ ions quench the calcein-dependent cytoplasmic and nuclear, but not mitochondrial, fluorescence, as they are excluded from the mitochondrial matrix owing to the barrier function of the inner mitochondrial membrane. Thus, the induction of MPT can be followed in cellula as a further drop in calcein-dependent fluorescence.53,54
HeLa cells transfected with a control siRNA (or mock-transfected) and loaded with calcein-Co2+ responded to the administration of 1 μM ionomycin (a ionophore that triggers MPT by promoting a mitochondrial Ca2+ overload) with a rapid decrease in calcein fluorescence. Such a drop could be fully prevented by the pre-administration of cyclosporine a (CsA, a chemical inhibitor of CYPD), confirming that it resulted from the opening of the PTPC (Fig. 2A and B). Importantly, the depletion of the FO c subunit (Fig. 2A), but not that of the F1 α subunit (Fig. S1D), inhibited MPT as efficiently as did the pre-administration of CsA. In contrast, HeLa cells overexpressing the FO c subunit underwent MPT in response to 1 μM ionomycin with a consistently accelerated kinetics (Fig. 2B).
MPT is known to result in the structural collapse of the mitochondrial network, (at least in part) owing to the colloidosmotic swelling of the mitochondrial matrix.9,13 In line with this notion, HeLa cells transfected with a control siRNA and with a mtGFP-coding construct responded to 1 μM ionomycin by undergoing mitochondrial fragmentation, as indicated by a consistent increase in the number of mtGFP+ objects per cell. Such an increase could be virtually abolished by the pre-administration of CsA as well as by the depletion of the FO c subunit (Fig. 2C), but not by that of the F1 α subunit (Fig. S1C). Conversely, mitochondrial fragmentation ensuing the opening of the PTPC in response to 1 μM ionomycin was largely aggravated by the overexpression of the c subunit of the FO ATP synthase, yet this phenomenon could be fully suppressed by CsA (Fig. 2D).
Taken together, these findings demonstrate that the c subunit of the FO ATP synthase plays a critical role in the CsA-dependent opening of the PTPC induced by cytosolic Ca2+ overload.
Together with the massive transfer of Ca2+ from the endoplasmic reticulum to the cytosol, oxidative stress, i.e., the condition in which reactive oxygen species (ROS) accumulate in spite of enzymatic and non-enzymatic antioxidant defense systems, is one of the best-characterized homeostatic perturbations that promotes PTPC opening in vitro and in vivo.9,21,23 Accordingly, the mitochondria of HeLa cells transfected with a control siRNA and exposed to a high dose (2 mM) of the prototypic pro-oxidant hydrogen peroxide (H2O2) underwent a rapid and irreversible depolarization. Conversely, the ΔΨm of HeLa cells depleted of the FO c subunit dissipated upon the administration of high-dose H2O2 with a consistently delayed kinetics (Fig. 3A). Along similar lines, while the mitochondria of mock-transfected HeLa cells responding to low-dose H2O2 (500 µM) depolarized in a relatively slow fashion, this process was dramatically accelerated in cells that overexpressed the c subunit of the FO ATP synthase (Fig. 3B). Consistent with these observations, cytochrome c could be readily detected by immunoblotting in cytosolic extracts of HeLa cells transfected with a control siRNA and treated with H2O2 (Fig. 3C), indicating that—in control conditions—these cells respond to oxidative stress by undergoing MOMP. Importantly, the amount of cytochrome c released into the cytosol by HeLa cells exposed to H2O2 was significantly reduced by the siRNA-mediated downregulation of the FO c subunit (Fig. 3C).
The ultimate functional consequence of MPT-driven MOMP is the activation of caspase-dependent and -independent executioner mechanisms of cell death.4,9 Indeed, approximately 25% and 40% of HeLa cells died upon exposure to ionomycin and H2O2, respectively, as indicated by the uptake of propidium iodide (PI), which can enter cells only upon the permeabilization of plasma membranes.55 Importantly, the depletion of FO c subunit, but not that of the F1 α subunit, considerably reduced the lethal effect of Ca2+ overload and oxidative stress in HeLa cells (Fig. 3D; Fig. S1E). The transfection-enforced overexpression of the FO c subunit per se induced some extent of cell death, yet failed to significantly aggravate the lethal response of HeLa cells to ionomycin and H2O2 (Fig. 3E), reinforcing the notion that these genetic and pharmacological interventions modulate a single functional module.
Finally, to test our observations in a more physiologically relevant model of MPT-driven cell death, we monitored the response of cultured rat cortical neurons (which mainly rely on mitochondria for ATP synthesis) to glutamate, a neurotransmitter that causes the potentially lethal release of Ca2+ ions from the endoplasmic reticulum (a phenomenon known as excitotoxicity).56,57 To this aim, rat cortical neurons were transfected with a GFP-encoding plasmid (to focus the analysis on transfected cells) together with either a control siRNA or with a siRNA mix targeting the FO c subunit, followed by a short exposure (30 min) to glutamate 48 h later. Thereafter, neurons were cultured in standard conditions for additional 24 h and eventually labeled with PI. In these conditions, approximately 20% of GFP+ neurons transfected with the control siRNA stained positively for PI, yet only about 10% of neurons efficiently receiving the siRNAs specific for the FO c subunit did so (Fig. 3F). Of note, the siRNAs originally designed to target the human form of the FO c subunit were efficient in cells from Rattus norvegicus as well, in spite of a few sequence misalignments (Fig. 3G).
Altogether, these data demonstrate that the c subunit of the FO ATP synthase regulates not only MPT but also its major functional consequences, including MOMP and cell death, both in glycolytic and respiratory cell models.
As a pathophysiologically relevant process, MPT can precipitate the unwarranted demise of post-mitotic cells in response to multiple stimuli. In particular, MPT has been involved in the acute loss of neurons and cardiomyocytes induced by ischemia/re-oxygenation and excitotoxicity.23,58-60 The molecular mechanisms that trigger the opening of the PTPC in these settings share a prominent oxidative component and the cytosolic accumulation of Ca2+ ions, which de facto are tightly linked to each other.61 During the last decades, several proteins have been suggested to constitute core components of the PTPC, including various isoforms of VDAC and ANT as well as CYPD, yet only the latter appears to be truly required for MPT in vivo.21,22,24-26 Here, we demonstrate that the c subunit of the FO ATP synthase is also necessary for MPT and its functional consequences, i.e., MOMP and cell death.
It might be argued that our findings do not reflect a direct implication of the FO c subunit in MPT, but rather alterations in the levels of ATP, ADP and inorganic phosphate, since all these metabolites (and many others) are known to influence the propensity of the PTPC to open.9 We are confident that this is not the case: since (1) the depletion of the FO c subunit in a near-to-purely glycolytic cell model, i.e., human cervical carcinoma HeLa cells,50 failed to alter the levels of mitochondrial ATP synthesis, yet inhibited MPT, mitochondrial fragmentation and cell death as triggered by cytosolic Ca2+ overload or oxidative stress, and (2) the downregulation of the F1 α subunit (which—similar to the FO c subunit—is required for the catalytic activity of the F1FO ATP synthase)62 completely failed to affect MPT and its functional consequences.
Experiments to elucidate the precise molecular mechanisms whereby the c subunit of the FO ATP synthase participates in MPT are underway. By ion exchange chromatography, we have succeeded in separating a multiprotein complex that contains ANT, VDAC1, CYPD, hexokinase, the FO c subunit as well as multiple F1 components (data not shown), in line with the existence of a supramolecular entity involving several constituents of the PTPC as well as the ATP synthasome.27,29-31 Now, it will be interesting to identify the direct physical interactors of the FO c subunit among the core PTPC components and its best known regulators (including pro- and anti-apoptotic BCL-2 family members as well as the oncosuppressor protein TP53).63,64 To test the actual pathophysiological relevance of our findings, it will be crucial to generate appropriate knockout models. Most likely, similar to the knockout of genes coding for other factors involved in mitochondrial respiration (e.g., cytochrome c),65 a whole body Atp5g1−/− genotype will turn out to be embryonic lethal. In addition, the murine genome appears to contain multiple Atp5g1-related coding sequences, further complicating the generation of bona fide Atp5g1−/− mice. The consistent degree of redundancy affecting PTPC-related proteins (many of which exist in at least three isoforms) has previously caused problems for the creation of Vdac- and Ant-deficient animals.24-26 Actually, the discovery of a fourth isoform of murine ANT (i.e., Ant4),66 has casted doubts on the conclusions of Kokoszka and colleagues, indicating that ANT would be dispensable for MPT.26 Irrespective of these unresolved issue, well-designed conditional knockout strategies and/or the identification of mutations by which the vital and lethal functions of the FO c subunit can be uncoupled may allow for the elucidation of exact contribution of this protein to MPT.
Finally, it is tempting to speculate, yet remains to be formally demonstrated, that agents targeting the FO c subunit and/or its interaction with other components of the PTPC might exert prominent cytoprotective effects by subverting MPT, MOMP and cell death as induced by pathogenic insults.
Unless otherwise noted, chemicals were purchased from Sigma-Aldrich, cell culture media and supplements from Gibco-Life Technologies and plasticware from Corning Life Sciences. Human cervical carcinoma HeLa cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 units/mL penicillin G sodium and 100 µg/mL streptomycin sulfate. Rat cortical neurons were obtained from 1–3-day-old rats, as previously described,67 and maintained in GlutaMax®-containing Neurobasal®-A medium supplemented with B-27® supplements, 100 units/mL penicillin G sodium and 100 µg/mL streptomycin sulfate.
RNA interference experiments were performed by transfecting cells with a commercial control siRNA (AllStars RNAi Controls, herein referred to as siSCR), with a validated siRNA specific for ATP5A1 (siATP5A1, sense 5′-GGCUGGAUUUGAAGCUUAAdTdT-3′) or with a mix of siRNAs targeting ATP5G1 (5′-GCUCUGAUCCGCUGUUGUAdTdT-3′), ATP5G1 (5′-CGGAGAUACUGACAGAUGAdTdT-3′) and ATP5G3 (5′-AGGGCUCUACGGUAUUUAAdTdT-3′), all purchased from Qiagen. siRNAs were transfected by means of the HiPerfect® transfection reagent, as per manufacturer’s instructions.68
For transient overexpression experiments, a pCMV6 entry-based plasmid coding for a MYC-tagged variant of ATP5G1 under the control of the CMV immediate early promoter (RC200292) was obtained from OriGene. For the quantification of mitochondrial ATP levels, a VR1012-based construct encoding a mitochondrially targeted variant of the Photinus pyralis luciferase under the control of the CMV immediate early promoter was employed.50 For the study of mitochondrial morphology, a pcDNA3-based plasmid coding for a mitochondrially targeted variant of GFP (mtGFP) under the control of the CMV immediate early promoter was used.52 For the quantification of cell death in neurons, a pcDNA3-based construct coding for GFP under the control of the CMV immediate early promoter was used.69 Plasmid (co-)transfections were performed via the standard Ca2+-phosphate technique.70
Dead cells were quantified by virtue of their ability to take up propidium iodide (PI), reflecting the breakdown of the plasma membrane.55,71 To this aim, HeLa cells were processed as indicated in the Tali® Apoptosis Kit (Molecular Probes-Life Technologies) and analyzed on a Tali® Image-Based cytometer (Invitrogen-Life Technologies). Conversely, rat neurons were stained with 1 μM PI (Molecular Probes-Life Technologies) for 5 min at room temperature (RT), then fixed with 4% paraformaldehyde (PFA) and stained with 1 μM Hoechst 33342 (Molecular Probes-Life Technologies) for 20 min at RT. Coverslips were then mounted on slides and analyzed on an Axiovert 200M microscope (Carl Zeiss) equipped with a 40× water immersion objective (N.A. 1.2, from Carl Zeiss) and a CoolSnap HQ CCD camera (Photometrics). Fifty random fields per condition were acquired in MetaMorph® (Molecular Devices) and images were analyzed with CellProfiler (Broad Institute) using a customized pipeline for the quantification of PI+ GFP+ cells.
HeLa Cells were loaded with 1 mM calcein acetoxymethyl ester and Co2+ as instructed by the Image-IT® LIVE Mitochondrial Transition Pore Assay Kit (Molecular Probes-Life Technologies). Cells were then imaged by means of 490 ± 20 nm excitation and 525 nm longpass emission filters on a Axiovert 200M fluorescence microscope equipped with a 40× water immersion objective (N.A. 1.2, from Carl Zeiss). Finally, images were analyzed with MetaMorph® and quenching rates were determined as the slopes of the fluorescence trace over a period of 60 sec post-stimulation.
Cells were loaded with 1 nM tetramethylrhodamine methyl ester (TMRM, from Molecular Probes-Life Technologies) in Krebs-Ringer buffer supplemented with 250 μM sulfinpyrazone, then placed in a humidified chamber at 37°C and imaged with a LiveScan Swept Field Confocal Microscope (Nikon Instruments Inc.) equipped with a 60× oil immersion (N.A. 1.4, from Nikon Instruments Inc.) every 30 sec for 30 min. TMRM fluorescence was analyzed by means of the NIS Elements software package (Nikon Instruments Inc.), and depolarization rates were defined as the slopes of the fluorescence trace over a period of 10 min post-stimulation.
HeLa cells expressing a mitochondrially targeted variant of the Photinus pyralis luciferase were perfused with a modified Krebs-Ringer buffer containing 125 mM NaCl, 5 mM KCl, 1 mM Na3PO4, 1 mM MgSO4, 1 mM CaCl2, 20 μM luciferin and 20 mM HEPES buffer (pH 7.4 at 37°C), and luciferin-dependent luminescence was monitored with a customized luminometer (Elettrofor), as previously described.50
HeLa cells expressing a mitochondrially targeted variant of GFP,69 were imaged with an IX-81 automated epifluorescence microscope (Olympus) equipped with a 60× oil immersion objective (N.A. 1.35, from Olympus) and an ORCA-R2 CCD camera (Hamamatsu Photonics K.K.). Selected cells were followed over time, and z-stacks were subjected to digital deconvolution by means of a Wiener deconvolution filter and a theoretical point-spread function provided by the Xcellence software (Olympus). GFP+ objects were quantified with the “3D object counter” plug-in of the open-source Fiji software (freely available at http://fiji.sc/), whereas 3D representations were obtained with the “3D Viewer” plug-in.
Immunofluorescence microscopy was performed according to standard procedures.72 Briefly, cells were fixed in 4% PFA for 20 min at RT, washed three times in PBS and permeabilized with 0.1% Triton X-100 (v:v in PBS) for 5 min at RT. Thereafter, unspecific binding sites were blocked by incubating cells in 2% powdered milk (w:v in PBS) for 1 h at RT. Cells were then incubated overnight at 4°C with primary antibodies specific for HSPD1 (1:100 in blocking buffer) or MYC (1:25 in blocking buffer) (both from Abcam). Finally, primary antibodies were revealed by means of appropriate AlexaFluor 488® and AlexaFluor 594® conjugates (Molecular Probes-Life Technologies). Images were acquired on an Axiovert 220 M microscope equipped with a 100× oil immersion Plan Neofluar® objective (N.A. 1.3, from Carl Zeiss) and a CoolSnap HQ CCD camera. Each field was acquired over 21 z-planes spaced by 0.5 μm, and z-stacks were deconvoluted with the “Parallel Iterative Deconvolution” plug-in of Fiji.
Immunoblotting was performed as previously described, with minor modifications.73 In brief, to obtain whole-cell extracts, cells were washed, harvested and lysed in RIPA buffer (50 mM TRIS-HCl pH 7.8, 150 mM NaCl, 1% IGEPAL CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM dithiothreitol) supplemented with 2 mM Na3VO4, 2 mM NaF, 1 mM phenylmethylsulfonyl fluoride and Complete Protease Inhibitor Cocktail® (Roche Diagnostics Corp.). Alternatively, cytosolic extracts were obtained by digitonin permeabilization, as previously described.74 Thereafter, protein extracts (30 µg/lane) were separated on precast 4–12% SDS-PAGE gels (Life Sciences), electrotransfered onto PVDF membranes (Bio-Rad) and probed with antibodies specific for the FO a subunit (Abcam), the FO c subunit (Abcam), the F1 α subunit (Abcam), cytochrome c (BD Biosciences) and VDAC1 (Abcam). An antibody specific for β actin (Sigma-Aldrich) was used to monitor equal lane loading. Finally, membranes were incubated with appropriate horseradish peroxidase-conjugated secondary antibodies (Southern Biotech), followed by chemiluminescence detection with the SuperSignal West Pico® reagent and CL-XPosure® X-ray films (both from Thermo Scientific-Pierce).
Unless otherwise indicated, assays were performed in triplicate independent instances, yielding comparable results. Data, which are presented as means ± SEM, were analyzed with Microsoft Excel (Microsoft Co.). Statistical significance was determined by means of ANOVA followed by two-tailed unpaired Student’s t-tests. p values < 0.05 were considered statistically significant.
We would like to thank Dr. Massimo Negrini, Giulia Fonti, Augusto Bevilacqua and Oliver Kepp for their extraordinary help. A.R. and C.G. are financed by the Italian Ministry of Health and Associazione Italiana per la Ricerca sul Cancro (AIRC), respectively. A.B. is supported by a Fondazione Italiana Sclerosi Multipla (FISM) fellowship (2010/B/1). S.P. is supported by a FISM training fellowship (2010/B/13). S.M. is supported by Fondazione Italiana per la Ricerca sul Cancro (FIRC) fellowship. M.R.W. is supported by the Polish National Science Center (UMO-2011/11/M/NZ3/02128) and BIO-IMAGing in Research Innovation and Education (FP7-REGPOT-2010-1). G.K. is supported by the European Commission (ArtForce); Agence National de la Recherche (ANR); Ligue contre le Cancer (Equipe labelisée); Fondation pour la Recherche Médicale (FRM); Institut National du Cancer (INCa); LabEx Immuno-Oncologie; Fondation de France; Fondation Bettencourt-Schueller; AXA Chair for Longevity Research; Cancéropôle Ile-de-France and Paris Alliance of Cancer Research Institutes (PACRI). P.P. is financed by AIRC, Telethon (GGP09128 and GGP11139B), the Italian Ministry of Education, University and Research and the Italian Ministry of Health.
No potential conflicts of interest were disclosed.
Supplemental materials may be found here:
Previously published online: www.landesbioscience.com/journals/cc/article/23599