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Logo of neurologyNeurologyAmerican Academy of Neurology
 
Neurology. Jan 8, 2013; 80(2): 196–202.
PMCID: PMC3589188
Randomized trial of minocycline in the treatment of HIV-associated cognitive impairment
Noeline Nakasujja, MD, PhD,corresponding author
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  • (1)NIH/NICHD 1R01HD070723-01 Role: Co- PI 2011 - 2016, (2)NIH/NICHD 1R01HD064416-01A1/Role: Co-PI,2011 - 2016, (3)NIH/NIMH R34 MH084782/Role:Co-PI 2010-2013, (4)NIMH MH083465 Role:Co-Investigator/coordinator 2008-2012, (5)R01 MH083568-01A2 Role:Coordinator/co-investigator 2008-2012, (6)NIH NINDS R21 NS065713-01A1 role:Co-investigator 2010-2012
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Sachiko Miyahara, PhD,
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  • Center for Biostatistics in AIDS Research at Harvard School of Public Health
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  • Harvard Center for AIDS Research
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Scott Evans, PhD,
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  • FDA, NIH, Genentech, HIV Neurobehavioral Research Center, Intermune, FzioMed, CIS Biotech, Pfizer, Roche, Partners Health (MGH), WeSTAT, Averion, Alcon, Harvard Medical School, Osaka University, Affymax, Boston University, Millenium, Novartis, Novel Therapeutics in HIV-associated Cognitive Disorders (Johns Hopkins), Initiative on Methods, Measurement, and Pain Assessment in Clinical Trials (IMPAACT)
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  • American Statistical Association, American Neurological Association, Statistical Society of Australia, Sunovion, Bohringer-Ingelheim, Interfarma, Deming Conference on Applied Statistics
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  • National Institute of Health (NIH) grants including the Neurologic AIDS Research Consortium grant NS32228 from NINDS and the Statistical and Data Management Center of the Adult AIDS Clinical Trials Group grant 1 U01 068634.
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Anthony Lee, MS,
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Seggane Musisi, MD,
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  • Non-profit entity: I sit on the Makerere University College Of Health Sciences Research And Ethics Committee
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  • Non-profit entity: 1.I received a return airticket and per diem for three days for participating in an NIMH writing workshop in Washington DC in April this year, 2. I received two airtickets to give talks at the Peter C Alderman Foundation Psycho-trauma Conferences in New York in November last year and Dar Es Salaam in July this year.
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  • I am the Eitor In Cheif of two Journals: 1. The African Journal of Traumatic Stress, 2010-2012, 2. The Makerere University Research Journal,2005-2012, I am on the Editorial Advisory Board of The African Journal Of Psychiatry. 2007-2012
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  • I teach at the Infectious Disease Institute,Mulago Hospital Hill, Kampala, where this research was carried out
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  • I have received the following research support: 1) 2011-2016 Ugandan Collaborator: Collaborative Hubs For International Research On Mental Health (19). Africa Focus On Interventional Research For Mental Health (AFFIRM). RFA-NIMH-11-070, 2) 2011-2012: Co-recipient of R01 supplementary grant: Outcome of Depression Treatment In HIV- Positive Patients In Care In Uganda. 3) 2008-2011: Co-recipient of R01 NIH grant: Depression effects and its treatment on Health Outcomes Among HIV-positive patients on ART in Uganda. 4) 2002-2010 : Recipient of two consecutive research grants from Government Of Sweden on The Sida/SAREC Makerere University - Karolinska Institutet Collaboration for PhD Research Training: i) Profiles Of Depression In The Lake Victoria Basin.ii) HIV-related Psychotic Disorders In The North Western Crescent Of The Lake Victoria basin. Musisi S as Senior Researcher (PI) and co-ordinator of the Mental Health Research Sub-program.
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  • I have received the following academically related grants: 1) 2011-2016 Ugandan Collaborator: Collaborative Hubs For International Research On Mental Health (19). Africa Focus On Interventional Research For Mental Health (AFFIRM). RFA-NIMH-11-070, 2) 2011-2012: Co-recipient of R01 supplementary grant: Outcome of Depression Treatment In HIV- Positive Patients In Care In Uganda. 3) 2008-2011: Co-recipient of R01 NIH grant: Depression effects and its treatment on Health Outcomes Among HIV-positive patients on ART in Uganda. 4) 2002-2010 : Recipient of two consecutive research grants from Government Of Sweden on The Sida/SAREC Makerere University - Karolinska Institutet Collaboration for PhD Research Training: i) Profiles Of Depression In The Lake Victoria Basin.ii) HIV-related Psychotic Disorders In The North Western Crescent Of The Lake Victoria basin. Musisi S as Senior Researcher (PI) and co-ordinator of the Mental Health Research Sub-program. I teach at the Infectious Disease Institute,Mulago Hospital Hill, Kampala, where this research was carried out
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Elly Katabira, MD,
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Kevin Robertson, PhD,
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Allan Ronald, MD,
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David B. Clifford, MD,
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  • Biogen Idec/Quintiles - DSMB for progressive MS trial 2011 -, Biogen Idec scientific advisor regarding PML 2005-2012, Genentech PML Advisory Board regarding PML 2007 - 2011, Millennium Consulting regarding PML 2007-2012, Bristol Meyers Squibb consulting 2008-2011, Genzyme consultant 2009-2011, Genzyme DSMB for CARE MS Study 2008-2011, Pfizer DMC for pregabalin neuropsthy study 2010 - 2011, Pfizer DMC for MAdCAM study 2011 -, Millennium - Chair PML IAC for clinical trials 2009 - 2012, BMS - advisory panel on complication from clincial trial 2010, IAS-USA speaker honorarium 2010, 2011, 2012, CMSC/ACTRIMS speaker honorarium 2012, Wyeth consultant 2011, Genentech - honorarium for participation in Translational Immunology Summit 2011, Jannsen - Expert consultant on PML 2011, Drinker, Biddle, Reath - Chair Scientific Advisory Committee of PML Consortium Millennium - ADCETRIS trial adjudication committee member 2012 -
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  • (1)Biogen - Study to test mefloquine as treatment for PML (2008-2010)
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  • NIH UO1 NS32228 - Neurologic AIDS Research Consortium (Principal Investigator) 1993 - 2012, NIH UO1 AI69495 - Washington University AIDS Clinical Trails Unit (Principal Investigator) 2006 - 2013, NIMH 22005 CHARTER Project (Washington U Site PI) 2007 - 2011, NIMH MH058076 HIV NeuroCognitive Disorderss: a randomized clinical trial of CNS-targeted HAART (Site PI) 2007-2011, NIH R21 3857-53187 (Fogarty Institute) Neurologic manifestations of HIV-2 in West Africa (2008-2011), NIH R01 NR012907 (NINR,Ances - PI), Co-investigator, 2010-2015, NIH R01 NR012907 (NINR, Ances - PI), Co-investigator, 2011-2016, DIAN TTU 12-243040 (Alzheimer Association, Bateman PI), Co-investigator, 2012-2016.
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and Ned Sacktor, MD
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Research Support, Government Entities:
  • AI-35042 (Margolick) 4/1/04 - 3/31/14 1.20 calendar month NIAID Multicenter AIDS Cohort Study, The major goals of this project are to characterize the incidence and course of neurological disorders associated with HIV infection, to determine risk factors and predictors of neurological disease, and to determine effects of antiretroviral treatment on development and course of neurologic disease. No scientific or budgetary overlap. N01MH22005 (Igor Grant) 3/12/03-7/31/10 0.24 calendar month NIMH Long term effects of potent antiretroviral therapy on HIV-induced nervous system disease (CHARTER), This project will address several critical questions relating to the use of HAART in treating HIV-dementia including: 1) the frequency of neurological deficits in HIV infection in the era of HAART; 2) the frequency of neuropathy and other complications of HAART; 3) the sensitivity and predictive value of magnetic resonance spectroscopy for HIV-Dementia. No scientific or budgetary overlap. MH71150 (Sacktor) 4/08/05-3/31/10 1.20 calendar month NIMH Oxidative Stress Markers and HIV Dementia, The major goal of this project is to define the association between markers of oxidative stress and HIV dementia and to determine whether markers of oxidative stress predict either the reversibility or progression of HIV dementia. No scientific or budgetary overlap. U01-NS32228 (Sacktor) 8/1/06-11/30/09 1.44 calendar month NINDS Neurologic AIDS Research Consortium - International Project - HIV Dementia in Uganda: Response to Highly Active Antiretroviral Therapy - Leadership Award, The major goal of this project is to determine whether highly active antiretroviral therapy improves neuropsychological Test performance among HIV+ individuals in Uganda with cognitive impairment. Neurologic AIDS Research Consortium - Minocycline Project - A Randomized, Double-Blind, Placebo-Controlled Trial of Minocycline for HIV-Associated Cognitive Impairment - Leadership Award The major goal of this project is to determine whether minocycline is safe and efficacious for the treatment of HIV-associated cognitive impairment. No scientific or budgetary overlap. P30-MH075673 (McArthur) 6/1/06-5/31/11 1.80 calendar month NIMH Center for novel therapeutics for HIV-associated cognitive disorders, The major goal of this Clinical Outcomes Core is to validate new outcome measures for epidemiological research and treatment trials and to provide the statistical and data management support for studies involving the Center grant resources. No scientific or budgetary overlap. MH058076 (Ellis) 9/28/06-7/31/11 1.44 calendar month NIMH HIV Neurocognitive disorders: a randomized clinical trial of CNS-targetted HAART The major goal of this project is to determine whether designing antiretroviral regimens according to a CNS-targeted strategy improves neurocognitive outcomes compared to a non-CNS targeted strategy among HIV+ individuals with cognitive impairment. No scientific or budgetary overlap. MH083465 (Sacktor) 9/01/08-3/31/10 1.20 calendar month NIMH HIV Dementia and Sensory Neuropathy in Uganda, The major goal of this project is to describe the epidemiology of HIV dementia and HIV sensory neuropathy among untreated HIV+ individuals with moderate-advanced immunosuppression. No scientific or budgetary overlap.
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  • NONE
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  • NONE
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From the College of Health Sciences (N.N., S.M., E.K.), Makerere University, Kampala, Uganda; Harvard School of Public Health (S.M., S.E., A.L.), Boston, MA; University of North Carolina (K.R.), Chapel Hill, NC; University of Manitoba (A.R.), Winnipeg, Canada; Washington University (D.B.C.), St. Louis, MO; and Johns Hopkins University School of Medicine (N.S.), Baltimore, MD.
corresponding authorCorresponding author.
Correspondence to Dr. Nakasujja: drnoeline/at/yahoo.com
Received April 21, 2012; Accepted August 27, 2012.
Objective:
To evaluate the efficacy and safety of minocycline in the management of HIV-associated cognitive impairment.
Methods:
We enrolled HIV-positive participants with a CD4 count of 250 to 500 cells/μL in a randomized, double-blind, placebo-controlled study. They received 100 mg of minocycline or matching placebo orally every 12 hours for 24 weeks. Cognitive function was measured using the Uganda Neuropsychological Test Battery Summary Measure (U NP Sum) and the Memorial Sloan-Kettering (MSK) scale. The primary efficacy measure was the 24-week change in an average of 9 standardized U NP Sum z scores.
Results:
Seventy-three participants were enrolled. Of these, 90% were female, 49% were between the ages 30 and 39 years, and 74% had 6 or more years of education. One participant had MSK score of stage 1 (i.e., mild HIV dementia), and 72 participants had MSK stage 0.5 (i.e., equivocal or subclinical dementia) at the baseline evaluation. The minocycline effect on the 24-week change of the U NP Sum compared with placebo was 0.03 (95% confidence interval −0.51, 0.46; p = 0.37).
Conclusion:
Minocycline was safe and well tolerated in HIV-positive individuals. However, it did not improve HIV-associated cognitive impairment.
Classification of evidence:
This study provides Class II evidence that 100 mg of minocycline given orally every 12 hours for 24 weeks had no significant effect compared with placebo in the improvement of cognitive function in antiretroviral therapy–naive, HIV-positive patients.
Although combination antiretroviral therapy (cART) improves cognitive impairment,1 the treatment response may be unsatisfactory or short-lived, or the agents may be poorly tolerated in doses adequate for CNS penetration.24 In Uganda as well as some other African countries, almost 50% of cART-eligible patients, even those with HIV cognitive impairment, remain untreated.58
Sequestration of the HIV virus in brain tissue may require an adjunct potent drug that can cross the blood-brain barrier with the ability to interfere with the inflammatory events of HIV infection and improve the management of HIV cognitive impairment. Minocycline would offer a unique therapeutic strategy for HIV-associated neurocognitive disorders because of its antiinflammatory and neuroprotective effects as has been noted in in vivo studies.914 To validate these findings, we hypothesized that minocycline treatment for 24 weeks would improve HIV-associated cognitive impairment and would be safe and well tolerated in individuals with HIV.
We thus conducted a phase I/II, randomized, double-blind, placebo-controlled study to assess the efficacy, tolerability, and safety of minocycline for the treatment of HIV-associated cognitive impairment in Uganda. In contrast to a prior study15 in the United States in which all HIV-positive individuals were receiving cART, this study was conducted in HIV-positive individuals who were not eligible for ART in the Uganda setting. Thus, the direct effect of minocycline and placebo in the absence of ART was examined in this study.
Recruitment/enrollment
During the screening process from April 2008 to September 2009, we selected every fifth patient out of a list of ART-naive, HIV-positive patients with a CD4 count in the range of 250 to 500 cells/μL who presented on each clinic day at the Infectious Diseases Clinic, Kampala, Uganda. This specialized outpatient HIV health unit offers free care and serves as a national referral center, providing specialist consultations for infected patients including those who are not responding to treatment from other health facilities. More than 9,000 HIV/AIDS clients have been registered since its establishment in 2004.
The screening included a measure for cognitive impairment using the 0- to 12-point International HIV Dementia Scale.16 Any person scoring ≤10 points was suspected of having HIV dementia as previously determined during the validation of the instrument. Two research assistants administered a detailed neuropsychological test battery that included the Timed Gait, Grooved Pegboard Dominant Hand, Grooved Pegboard Nondominant Hand, Color Trails 1, Color Trails 2, Symbol Digit, World Health Organization–University of California at Los Angeles (WHO-UCLA) Verbal Learning Test trial 5, WHO-UCLA Verbal Learning Test delayed recall, and Digit Span forward and Digit Span backward.
The selection criteria for the study participants are shown in table 1. The study had 2 steps: step 1 was the double-blind phase in which individuals received minocycline or placebo for 24 weeks; step 2, an optional open phase, was available for those who completed step 1 and every participant who agreed to enroll received minocycline for 24 additional weeks.
Table 1
Table 1
Eligibility criteria
Standard protocol approvals, registrations, and patient consents
The study clinical trial identifier number in ClinicalTrials.gov was NCT00855062.
The Makerere University School of Medicine Research and Ethics Committee as well as the Uganda National Council for Science and Technology approved the use of human subjects. All participants gave informed consent before enrollment into the study.
Intervention
In this double-blind, placebo-controlled study, we randomized 73 participants to receive either 100 mg of minocycline orally every 12 hours or matching placebo orally every 12 hours daily for 24 weeks. We performed a neurologic examination and safety laboratory tests including serum chemistry profiles (electrolytes, liver function tests), hematology, and CD4 lymphocyte counts at screening and weeks 4, 12, and 24. A neuropsychological test battery was performed at screening, entry, 12 weeks, and 24 weeks. The presence of depression symptomatology was assessed with the Center for Epidemiologic Studies Depression Scale.17 We measured the participants' functional performance with the Karnofsky scale and Instrumental Activities of Daily Living (IADL). Individuals who had higher scores on the Karnofsky scale at subsequent visits compared with the baseline score were considered to be “better” in their functional performance. The level of cognitive function was scored using the Memorial Sloan-Kettering scale.
The participants were randomly assigned to a treatment group based on a preassigned randomization list. The list was kept by the study pharmacist who had no access to the patients' clinical evaluations both at baseline and through follow-up. Once a patient had been screened and found fit for the study, the research assistant would notify the pharmacist of the participant. The pharmacist would then assign a number in the randomization list as appropriate.
Outcome measures
The primary objective of the study was the change in neurocognitive performance from baseline to 24 weeks as measured by the Uganda Neuropsychological Test Battery Summary Measure (U NP Sum). Each test score was standardized to normal, 1, or 2 SDs from the mean in comparison to normative values of an age-, sex-, and education-matched general non-HIV population.18 The U NP Sum was defined as the mean of the z scores of the following tests: Grooved Pegboard Dominant Hand, Grooved Pegboard Nondominant Hand, Color Trails 1, Color Trails 2, Symbol Digit, WHO-UCLA Verbal Learning Test trial 5 total, WHO-UCLA Verbal Learning Test delayed recall, Digit Span forward, and Digit Span backward. The secondary objective of the study was the measure of safety including the frequency of adverse events and abnormal results on laboratory tests, and changes over time in vital signs and symptoms.
Statistical methods
The primary analysis followed the intention-to-treat principle, and all 73 randomized participants were included. The number needed to treat was 25. Because the primary end point was a continuous variable, a new dichotomous variable (0 = did not reach a clinically meaningful cutoff of 0.5; 1 = reached the cutoff) was created and the proportions of participants who reached the clinically meaningful cutoff from the minocycline and control groups were used to calculate the number needed to treat.
The study sample size was arrived at by assuming that both arms (minocycline and placebo) would have an SD of 0.7 in the 24-week change in U NP Sum, a 17% loss-to-follow-up rate, 10% noncompliance, and Pitman efficiency of 0.864. To be able to detect a difference of 0.5 (a minimum clinically relevant difference) in the 24-week U NP Sum changes with 85% power and a type I error rate of 0.05 required 100 subjects (50 per group).
Because 19% of participants had missing 24-week U NP Sum for various dropout reasons, the missing values were imputed by a multiple regression imputation method. Once all values were imputed, a regression model was fit to estimate the efficacy of minocycline on the 24-week change of U NP Sum, adjusting for the baseline U NP Sum. Two different sensitivity analyses were also conducted: 1) a linear regression model based on observed outcomes, and 2) a mixed model with all observed and repeatedly measured outcomes. All secondary analyses were based on observed data. For continuous outcomes, linear regression models adjusting for a baseline score were used, and for categorical outcomes, logistic regression models were used. For both primary and secondary analyses, the Bonferroni correction was applied on type I error to adjust for multiplicity.
We also examined the primary objective using the reliable change index (RCI). The RCIs between the minocycline and placebo groups were compared using a z test to determine the effect on the 24-week change of U NP Sum.
Classification of evidence
The primary research question for this study was: Does minocycline improve cognitive function among HIV-positive individuals? This intervention study provides Class II evidence that minocycline treatment of 100 mg given orally every 12 hours for 24 weeks does not improve HIV-associated cognitive impairment among HIV-positive individuals with a CD4 count of 250 to 500 cells/μL (minocycline effect on the 24-week change of cognitive function compared with placebo was −0.03, 95% confidence interval [CI] −0.51 to 0.46; p = 0.37).
The study was initially planned to enroll 100 participants. After half the participants completed the double-blind phase, an interim analysis of primary and secondary outcome measures was performed and reviewed by the Neurological AIDS Research Consortium Data Safety and Monitoring Board (DSMB). The DSMB recommended early termination of the study because of outcome futility, and participants were informed and discharged after completing the final study visits. The overall accrual and study step discontinuation in each study arm are summarized in the figure. Of 73 randomized participants, 52 (71%) completed the 24-week protocol, and 28 (38%) completed the 48-week protocol. As shown in table 2, 90% of the participants were women. The modal age group was 30 to 39 years (49%) and most (53%) had received 6 to 10 years of education. The treatment arms were similar in baseline characteristics. Only 1 participant in the minocycline arm had stage-1 dementia (i.e., mild HIV dementia), and the remainder of the 72 participants had stage 0.5 (i.e., equivocal or subclinical dementia). The minocycline effect on the 24-week change of the U NP Sum compared with placebo was −0.03 (95% CI −0.51, 0.46; p = 0.37) (table 3). The upper CI did not reach the prespecified clinically meaningful cutoff point of 0.5, and this result rules out a significant minocycline effect compared with placebo. The effect of minocycline on the 24-week change in the CD4 cell count after adjusting for the baseline CD4 count was 8.11 (95% CI −27.23, 43.45; p value = 0.65). The mean CD4 count decreased by 25 cells/μL in the minocycline arm and by 28 cells/μL in the placebo arm; however, this was not significantly different. Two participants (6%) in the placebo arm and 1 participant (3%) in the minocycline arm had improved score on the Karnofsky functional performance scale at week 24 compared with baseline (95% CI 0.04, 0.06; p = 0.61). Four participants (14%) in the minocycline arm and 3 participants (12%) in the placebo arm had an improved IADL score at week 24 compared with their baseline. The treatment effect on the IADL (estimated by odds ratio) was 1.28 (95% CI 0.26, 6.34; p = 0.76). The 95% CI of being “better" for the minocycline arm was 0.05, 0.37 and 0.03, 0.34 for the placebo arm.
Figure
Figure
Participant flow chart
Table 2
Table 2
Baseline characteristics of study participants
Table 3
Table 3
Neuropsychological standardized z-score change and minocycline effect at 24 weeks
None of the secondary cognitive outcome measures showed a difference between the 2 treatment arms with one exception. The 24-week change of the delayed verbal memory recall in a general linear model adjusting for baseline neuropsychological test score showed a benefit for the placebo arm of −0.718 (95% CI −1.35, −0.08; p value = 0.03).
The mean (SD) RCI for the minocycline and placebo groups was 0.84 (1.43) and 0.95 (1.28), respectively. The minocycline effect on the 24-week change of U NP Sum compared with the placebo effect was not statistically significant (p = 0.76). There were no significant differences between the minocycline and placebo arms on the subjective and performance-based functional measures, measures of mood, or viral load assessments.
Safety
Of note were 7 cases (19.4%) of grade-2 hyperpigmentation that occurred among patients taking minocycline. This usually happened by week 12; however, the condition would resolve by the subsequent clinic visits. Two patients experienced a grade-4 rash related to the study drug. The probability of not experiencing any signs and symptoms by week 24 was higher for the minocycline group but was not significantly different from the placebo arm using the log-rank test (p = 0.58).
There was 1 death that was unrelated to study drug. This patient had only 11% adherence to study drug at the end of the fourth week after randomization. In the sixth week, the study drug was withdrawn after continuing adherence problems. She was later diagnosed to be coinfected with pulmonary tuberculosis, to which she later succumbed.
In this randomized, double-blind, placebo-controlled study, 100 mg of minocycline given orally every 12 hours had no significant effect over placebo in the improvement of cognitive function in ART-naive, HIV-positive patients. The drug, however, was safe and well tolerated. Apart from the delayed verbal recall test, there were no differences detected among the 2 arms in the change scores for the secondary outcome measures of neuropsychological test performance. The level of functional improvement between the 2 study arms was similar. These results are consistent with a second trial of minocycline for the treatment of HIV-associated cognitive impairment recently performed in HIV-positive individuals with neurocognitive impairment on cART.15
Cognitive impairment as seen in the study population continues to be an important manifestation of HIV infection, affecting 40% to 60% of HIV seropositive individuals even after the initiation of highly active antiretroviral therapy.19 The cognitive impairment observed in this particular study group could also have been due to non-HIV comorbid infections or conditions. Hepatitis C infection is one such condition but it is rare among HIV-positive individuals in Uganda, with a prevalence of 3%,20,21 and thus an unlikely cause for the observations made. Tuberculosis with CNS involvement can affect the level of consciousness and consequently the level of cognitive function.22 However, the screening criteria for this particular study excluded all subjects who had evidence of an active tuberculous infection. Nutritional deficiencies including micronutrients can be a primary causative factor in HIV disease progression, thereby contributing to cognitive dysfunction.23 The population in this study, however, was mostly observed to be adequately nourished.
There are usually differences in response to pharmacologic agents among males and females. Our study population was mostly female and thus the low number of males may have obscured sex effects of performance on tests as well as the effects of the investigational drug.
Other factors such as depression or side effects from medications have also been associated with cognitive impairment.24 We screened for depression with the Center for Epidemiologic Studies Depression Scale, and the study sample had equal distribution of individuals with the same range of symptoms within the placebo and minocycline arms. All study participants were prescribed trimethoprim-sulfamethoxazole for prophylaxis in compliance with Uganda treatment guidelines. This drug has no known effects on cognitive function.
The early penetration of the HIV virus into brain tissue leads to sequestration of the virus,25 and therefore the minocycline dose of 200 mg/d used in the study may have been inadequate to change inflammatory events or provide neuroprotective properties within the CNS even though minocycline has relatively good brain penetration.26,27 In addition, neuronal injury or dysfunction occurs early in HIV infection, and may be unresponsive to potential antiinflammatory or neuroprotective effects of minocycline started during late-stage HIV infection. Surrogate markers such as neuroimaging or CSF biomarkers may be needed to evaluate early evidence of neuroprotection within the CNS rather than the clinical neuropsychological tests and functional outcomes used in this evaluation. The duration of observation for cognitive change was relatively short; evaluations conducted for a longer period of time could possibly yield different results. The neurocognitive testing was conducted 3 times in the 24 weeks, and this may have resulted in practice effects that may have contributed to the improvement in both groups and thus may have obscured differences between the minocycline and placebo groups. Unfortunately, this kind of evaluation was not designed for this study. Interestingly, the probability of individuals not experiencing signs and symptoms of “any cause” was higher in the minocycline arm compared with placebo by week 24. The signs and symptoms included any clinical event such as cough, fever, and nausea, among others, and the patients seemed to fare better on the treatment. This may be secondary to the antiinflammatory properties offered by the drug.28
Minocycline has been shown to have a neuroprotective effect in animal models for a variety of neurologic conditions.13,29 In humans, the drug has been shown to have therapeutic benefit in clinical trials of multiple sclerosis and stroke.10,30 However, in a study of 412 individuals with amyotrophic lateral sclerosis, participants taking minocycline deteriorated more quickly as measured by the amyotrophic lateral sclerosis functional rating scale of gross and fine motor tasks, bulbar function, and respiratory function compared with those receiving placebo.31
Supplementary Material
Abstract in Arabic
ACKNOWLEDGMENT
The authors thank Alice Namudde, Hope Mackline, and Charles Ssebunya for the data collection. The authors extend their gratitude to the study participants for their willingness to participate in the study.
GLOSSARY
cARTcombination antiretroviral therapy
CIconfidence interval
DSMBData Safety and Monitoring Board
IADLInstrumental Activities of Daily Living
RCIreliable change index
U NP SumUganda Neuropsychological Test Battery Summary Measure
WHO-UCLAWorld Health Organization–University of California at Los Angeles

Footnotes
Go to Neurology.org for full disclosures. Funding information and disclosures deemed relevant by the authors, if any, are provided at the end of the article.
AUTHOR CONTRIBUTIONS
N. Nakasujja contributed to the design, conduction of the study, interpretation of data, and manuscript preparation. S. Miyahara, S. Evans, and A. Lee contributed to the data analysis, interpretation of the data and manuscript writing. S. Musisi and E. Katabira contributed to the conduction of the study, interpretation of data, and manuscript writing. K. Robertson and A. Ronald contributed to the design of the study, interpretation of data, and manuscript writing. D.B. Clifford contributed to the conduction of the study, interpretation of data, and manuscript writing. N. Sacktor contributed to the design and conduction of the study, interpretation of data, and manuscript writing.
STUDY FUNDING
The study was supported by the Neurologic AIDS Research Consortium, NS32228.
DISCLOSURE
N. Nakasujja receives support from the NIH (NIMH, NICHD). S. Miyahara reports no disclosures. S. Evans receives support from the NIH and has had consultancies (e.g., DSMB, educational presentations, advisory roles) with Millenium, Pfizer, Averion, Massachusetts General Hospital, Roche, Harvard Clinical Research Institute, IMPAACT, InterMune, HANC, Genentech, HNRC, CIS Biotech, Affymax, FzioMed, Novartis, Alcon, Sunovion, Boehringer-Ingelheim, ASA, ANA, BIDMC, Osaka University, Statistical Society of Australia, Interfarma, BASS, Society for Clinical Trials, FDA, PERI, Harvard Medical School, the Graybill Conference, the Deming Conference on Applied Statistics, MBSW, New Jersey Chapter of ASA, NIH, Merck, Schering-Plough, and the Center for Novel Therapeutics in HIV-Associated Cognitive Disorders (Johns Hopkins). A. Lee receives support from NIH (NIAID). S. Musisi, E. Katabira, and K. Robertson receive support from NIH (NIMH). A. Ronald reports no disclosures. D.B. Clifford serves on Data Safety Boards for Biogen, GSK, Millennium, Genzyme, Genentech, and Pfizer. He has been a consultant to Amgen, Brinker Biddle and Reath (PML Consortium), Genentech, Genzyme, Bristol-Myers Squibb, Johns Hopkins University, Millennium, Biogen Idec, Janssen, and Pfizer. He has received research support from Biogen Idec, NeurogesX, Tibotec, and Pfizer. He has received speaking fees from University of Kentucky and CMSC/ACTRIMS. N. Sacktor receives support from the NIH (NIMH, NINDS, NIA, NIAID). Go to Neurology.org for full disclosures.
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