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Genome Announc. 2013 Jan-Feb; 1(1): e00160-12.
Published online 2013 February 21. doi:  10.1128/genomeA.00160-12
PMCID: PMC3587934

Draft Genome Sequence of Enterococcus faecalis PC1.1, a Candidate Probiotic Strain Isolated from Human Feces

Abstract

Enterococcus faecalis is commonly isolated from the gastrointestinal tract of healthy infants and adults, where it contributes to host health and well-being. We describe here the draft genome sequence of E. faecalis PC1.1, a candidate probiotic strain isolated from human feces.

GENOME ANNOUNCEMENT

Enterococcus faecalis is a facultative anaerobic bacterium that is a primary colonizer of the infant gastrointestinal tract and is also prevalent in the adult gastrointestinal tract (13). E. faecalis is typically considered to be a beneficial gut bacterium that has been shown to be capable of modulating the activities of specific animal pathogens (4, 5) and the host immune response (6, 7); consequently, several strains have been applied as probiotics for the maintenance of a healthy gut and the treatment of gut diseases, including diarrhea, irritable bowel syndrome, and ulcerative colitis (810). Despite this, E. faecalis is now also widely recognized as a potential gut pathogen that is capable of compromising the epithelial barrier (11), inducing inflammation in mouse models of experimental colitis (12, 13) and causing chromosomal instability in colonic epithelial cells (14, 15). Key intraspecies variations with relevance to pathogenesis exist, and thus, we sought to elucidate further the genomic potential of an E. faecalis isolate from a healthy human gut and to assess its potential as a probiotic candidate. We describe here the draft genome sequence of E. faecalis PC1.1, isolated as part of the Australian Human Gut Microbiome Project.

E. faecalis PC1.1 was isolated from a pooled fecal sample collected from healthy human subjects by plating aerobically on brain heart infusion (BHI) medium. Genomic DNA was prepared, and a 454 Life Sciences Genome Sequencer (GS) FLX system was used at the J. Craig Venter Institute (JCVI) to generate 2,753,924 bp of DNA sequence at 30× coverage. The individual sequence reads were assembled using the Newbler Assembler v2.3 to generate 79 contigs. The contig N50 was approximately 57.3 kb and the largest contig assembled was approximately 132.5 kb. Finally, the DNA sequences were annotated using the JCVI prokaryotic annotation pipeline.

The draft genome has a G+C content of 37.6% and contains 2,673 genes, with 2,619 protein-coding genes and 54 structural RNAs. As anticipated, the annotated genome of E. faecalis PC1.1 revealed the presence of factors relevant to the colonization of and persistence in the human gut, including protein orthologs with putative roles in mediating adhesion to host structural factors, including collagen, fibronectin, and fibrinogen. In addition, we identified a putative lectin with a suspected role in mediating adhesion to host structural glycans and also several proteins with likely roles in harvesting host glycans. The E. faecalis PC1.1 genome encodes a putative gelatinase and serine proteinase; however, it does not appear to encode an enterococcal surface protein or enterococcal cytolysin, which have been shown previously to be important for virulence.

This preliminary study provides insight into the genomic factors that contribute to the colonization of and persistence in the gut. Nontoxigenic Clostridium difficile can effectively colonize the gut of hamsters and humans and prevent colonization by toxigenic C. difficile strains (reviewed in reference 16); further studies will be necessary to determine the probiotic potential of E. faecalis PC1.1, including its ability to similarly prevent colonization and/or modulate the activities of pathogenic microbes and to productively influence the immune response.

Nucleotide sequence accession numbers.

This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession no. ADKN00000000. The version described here is the first version, ADKN01000000.

ACKNOWLEDGMENTS

This research was performed as part of CSIRO’s contribution to the International Human Microbiome Consortium. This project was supported by the Commonwealth of Australia under the International Science Linkages program, by CSIRO's Transformational Biology Capability Platform in support of the Preventative Health Flagship Research Program and NIH award number U54AI084844 to Karen E. Nelson. We gratefully acknowledge the support provided by CSIRO’s OCE Science Leader scheme (to M.M.) and the OCE Postdoctoral Fellow program (awarded to E.K.).

We thank CSIRO Human Nutrition for collecting the fecal samples and Seungha Kang and Desma Grice for providing critical reading and suggestions to improve the manuscript.

Footnotes

Citation Ó Cuív P, Klaassens ES, Smith WJ, Mondot S, Durkin AS, Harkins DM, Foster L, McCorrison J, Torralba M, Nelson KE, Morrison M. 2013. Draft genome sequence of Enterococcus faecalis PC1.1, a candidate probiotic strain isolated from human feces. Genome Announc. 1(1):e00160-12. doi:10.1128/genomeA.00160-12.

REFERENCES

1. Hopkins MJ, Macfarlane GT, Furrie E, Fite A, Macfarlane S. 2005. Characterisation of intestinal bacteria in infant stools using real-time PCR and northern hybridisation analyses. FEMS Microbiol. Ecol. 54:77–85. [PubMed]
2. Kirtzalidou EI, Mitsou EK, Pramateftaki P, Kyriacou A. 2012. Screening fecal enterococci from Greek healthy infants for susceptibility to antimicrobial agents. Microb. Drug Resist. 18:578–85. [PubMed]
3. Noble CJ. 1978. Carriage of group D streptococci in the human bowel. J. Clin. Pathol. 31:1182–1186. [PMC free article] [PubMed]
4. Cebrián R, Baños A, Valdivia E, Pérez-Pulido R, Martínez-Bueno M, Maqueda M. 2012. Characterization of functional, safety, and probiotic properties of Enterococcus faecalis UGRA10, a new AS-48-producer strain. Food Microbiol. 30:59–67. [PubMed]
5. Soerjadi AS, Lloyd AB, Cumming RB. 1978. Streptococcus faecalis, a bacterial isolate which protects young chickens from enteric invasion by salmonellae. Aust. Vet. J. 54:549–550. [PubMed]
6. Are A, Aronsson L, Wang S, Greicius G, Lee YK, Gustafsson JA, Pettersson S, Arulampalam V. 2008. Enterococcus faecalis from newborn babies regulate endogenous PPARγ activity and IL-10 levels in colonic epithelial cells. Proc. Natl. Acad. Sci. U. S. A. 105:1943–1948. [PubMed]
7. Wang S, Ng LH, Chow WL, Lee YK. 2008. Infant intestinal Enterococcus faecalis down-regulates inflammatory responses in human intestinal cell lines. World J. Gastroenterol. 14:1067–1076. [PMC free article] [PubMed]
8. Chen CC, Kong MS, Lai MW, Chao HC, Chang KW, Chen SY, Huang YC, Chiu CH, Li WC, Lin PY, Chen CJ, Li TY. 2010. Probiotics have clinical, microbiologic, and immunologic efficacy in acute infectious diarrhea. Pediatr. Infect. Dis. J. 29:135–138. [PubMed]
9. Enck P, Zimmermann K, Menke G, Müller-Lissner S, Martens U, Klosterhalfen S. 2008. A mixture of Escherichia coli (DSM 17252) and Enterococcus faecalis (DSM 16440) for treatment of the irritable bowel syndrome—a randomized controlled trial with primary care physicians. Neurogastroenterol. Motil. 20:1103–1109. [PubMed]
10. Tsuda Y, Yoshimatsu Y, Aoki H, Nakamura K, Irie M, Fukuda K, Hosoe N, Takada N, Shirai K, Suzuki Y. 2007. Clinical effectiveness of probiotics therapy (bio-three) in patients with ulcerative colitis refractory to conventional therapy. Scand. J. Gastroenterol. 42:1306–131. [PubMed]
11. Steck N, Hoffmann M, Sava IG, Kim SC, Hahne H, Tonkonogy SL, Mair K, Krueger D, Pruteanu M, Shanahan F, Vogelmann R, Schemann M, Kuster B, Sartor RB, Haller D. 2011. Enterococcus faecalis metalloprotease compromises epithelial barrier and contributes to intestinal inflammation. Gastroenterology 141:959–971. [PubMed]
12. Balish E, Warner T. 2002. Enterococcus faecalis induces inflammatory bowel disease in interleukin-10 knockout mice. Am. J. Pathol. 160:2253–2257. [PubMed]
13. Kim SC, Tonkonogy SL, Albright CA, Tsang J, Balish EJ, Braun J, Huycke MM, Sartor RB. 2005. Variable phenotypes of enterocolitis in interleukin 10-deficient mice monoassociated with two different commensal bacteria. Gastroenterology 128:891–906. [PubMed]
14. Huycke MM, Abrams V, Moore DR. 2002. Enterococcus faecalis produces extracellular superoxide and hydrogen peroxide that damages colonic epithelial cell DNA. Carcinogenesis 23:529–536. [PubMed]
15. Wang X, Huycke MM. 2007. Extracellular superoxide production by Enterococcus faecalis promotes chromosomal instability in mammalian cells. Gastroenterology 132:551–561. [PubMed]
16. Gerding DN. 2012. Clostridium difficile infection prevention: biotherapeutics, immunologics, and vaccines. Discov. Med. 13:75–83. [PubMed]

Articles from Genome Announcements are provided here courtesy of American Society for Microbiology (ASM)