(A) Cumulative analysis of the 5′ ends of unique CIP-TAP (orange) and CIP-PNK (green) sequences with respect to the YRNU motif of a consensus 21U-RNA locus. The scale (linear) is shown. The red segment preceded by U indicates the mature 21U-RNA.
(B) Graph showing the length distribution of CIP-TAP/csRNA reads (orange) and CIP-PNK/21U-RNA reads (green) mapping to 21U-RNA loci.
(C) Graph of csRNA levels plotted against corresponding 21U-RNA levels for annotated 21U locus. The points in red indicate previously annotated 21A/G/C piRNAs. Points near the X-axis (under the bracket) include 22G-RNAs previously mis-annotated as piRNAs.