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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Innate Immun. Author manuscript; available in PMC Feb 16, 2013.
Published in final edited form as:
PMCID: PMC3574571
NIHMSID: NIHMS407853
Lymph node macrophages
Elizabeth E. Gray and Jason G. Cyster*
Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco, 513 Parnassus Ave., San Francisco, CA 94143-0414
*Address correspondence to: Jason G Cyster, Jason.Cyster/at/ucsf.edu
Lymph node (LN) macrophages have long been known for their efficient uptake of lymph-borne antigens. A convergence of studies on innate and adaptive immune responses has led to exciting recent advances in understanding their more specialized properties: presenting antigens to B cells, dendritic cells and T cells, producing trophic factors and cytokines, and, remarkably, being permissive for viral infection, a property critical for mounting anti-viral responses. LN macrophages have been traditionally divided into subsets based on their subcapsular sinus and medullary locations. Here we classify LN macrophages into three subsets: subcapsular sinus macrophages (SSMs), medullary sinus macrophages (MSMs) and medullary cord macrophages (MCMs). We review the literature regarding the roles of these cells in innate and adaptive immune responses and requirements for their development. We also discuss challenges associated with their purification as well as the existence of additional heterogeneity among LN macrophages.
Keywords: Macrophage, phagocyte, lymphatic sinus, sialoadhesin/CD169, F4/80, Mac1, membrane blebs, antigen presentation
Macrophages are classically defined as highly phagocytic cells that internalize and degrade particles and pathogens and release mediators that alert the adaptive immune system to invaders. They are bone marrow derived and many are dependent on colony stimulating factor-1 (CSF1) for their development [1,2]. Although traditionally thought to develop from blood monocytes, recent studies indicate that this may not be the case for all macrophages [3]. All tissues in the body contain macrophages, which frequently take on specialized properties that allow them to support functions uniquely required in their tissue of residence. Thus, LN macrophages are distinct from those found elsewhere in the body and support LN-specific as well as more general immune functions.
Many LN macrophages are directly exposed to lymph fluid. Typically several afferent lymphatics enter the capsule of a single LN and afferent lymph passes into the narrow subcapsular sinus (SCS) that overlies the lymphocyte-rich cortex (Fig. 1). Lymph leaves the LN on the opposite (hilus) side via the efferent lymphatic vessel. The region of the LN adjacent to the efferent vessel, known as the medulla, contains numerous irregularly shaped and interconnected sinuses. Lymph travels from the SCS through this medullary sinus network before collecting in the efferent vessel. The tissue between the medullary sinuses, which often appears cylindrical in cross-section, is referred to as medullary cords (Fig. 1). LNs in humans are considerably larger than in mouse and rat and trabecular sinuses radiate from the SCS through the parenchyma to the medullary sinuses, providing an additional path for lymph flow [4,5].
Figure 1
Figure 1
Schematic of LN showing major zones and lymphatic sinuses and suggested nomenclature for macrophage subsets
LN macrophages have long been recognized based on their sinus-associated location and early uptake of lymph-borne antigens. Early anatomical studies led to the description of macrophages in the floor of the SCS, overlying follicles, that were quick to capture small amounts of lymph-borne substances, but appeared poorly phagocytic [68]. The macrophages associated with the medullary sinuses, by contrast, acquired large amounts of material that was readily observable within phagolysosomes [710]. The properties of these two types of sinus-associated macrophages will be discussed in detail below. The medulla also contains macrophages that reside outside the sinuses, situated within the medullary cords. Another macrophage type present in reactive LNs, though not unique to these organs, is the tingible body macrophage, which has a specialized role in the uptake of apoptotic cells within germinal centers.
Just as LNs filter lymph, the spleen filters blood. Terminal arterioles in the spleen release blood into a macrophage-lined space termed the marginal sinus. Two major types of macrophages are associated with this region: marginal metallophillic macrophages (MMMs) and marginal zone macrophages (MZMs) [11]. These cells share a number of properties with the sinus associated LN macrophages [12], and insights that have been made through studies in the spleen that are likely to apply to LN macrophages will be discussed.
Surface phenotyping with monoclonal antibodies has been an important approach in identifying and characterizing macrophage subsets. Perhaps the best-defined marker that is present on many macrophage types is the integrin Mac1 (also known as αM/β2, CD11b/CD18 or complement receptor-3) [1]. Mac1 is present on a variety of other immune cell types, for example being abundant on granulocytes, dendritic cells (DCs) and subsets of B cells. Two other markers, CD169 and F4/80, have also proved valuable in studying macrophage subsets.
CD169, also known as sialoadhesin or siglec-1, is the largest member of the sialic acid binding lectin (Siglec) family [13]. It is present on resident bone marrow macrophages, LN SCS and medullary macrophages and splenic MMMs as well as subsets of macrophages in non-lymphoid tissues, and it may be induced on monocytes under some inflammatory conditions [12,13]. It binds a number of heavily O-glycosylated proteins including CD43 and Mucin-1 [13]. Sialic acid-independent interactions occur with the cysteine-rich domain of the mannose receptor (MR) and with macrophage galactose-type N-acetylgalactosamine-specific lectin-1 (MGL1) [13,14].
F4/80 (EMR1) is a member of the EGF-7TM family of leukocyte heptahelical molecules [15]. While it has served as an excellent marker for identifying subsets of macrophages, its function is still largely obscure [15]. It is present on a wide range of macrophage types, including LN medullary macrophages, splenic red pulp macrophages, bone marrow macrophages, sinusoidal macrophages in the liver (Kupffer cells), and on extravascular macrophages in tissues, including microglia; it is dim on inflammatory monocytes and some DCs and can be expressed on eosinophils [15].
In this review we discuss current understanding regarding the major macrophage types uniquely present in LNs. We refer to the macrophage subsets from an anatomical perspective as these cells have limited motility and their positioning is tightly linked to their function. One major type of macrophage has been described in the SCS, and these cells are referred to as SSMs (subcapsular sinus macrophages). In an effort to simplify what can otherwise be a complex (and difficult to remember) surface marker-based nomenclature, and to highlight what we argue will be an important sub-classification of medullary macrophages, we refer to medullary sinus macrophages as MSMs and medullary cord macrophages as MCMs.
SSMs in rodents stud the lymphatic lining that overlies B cell follicles, forming a dense cellular sheet [1619]. In the mouse they are CD169hi, Mac1+ and lack expression of F4/80 [1720]. Many SSMs have a ‘head’ that projects into the sinus and long ‘tail’ processes that extend into the underlying follicle, with the ‘neck’ tightly inserted across the lymphatic lining cell layer (Fig. 1). Real-time imaging shows these cells to be resident in this transcellular position and, in contrast to the dendrites of DCs, their long tail processes are rather stable, perhaps attached to the extracellular matrix [1720]. The cues promoting their positioning are not defined, but lymph-derived factors seem likely to be involved since occlusion of afferent lymphatics leads to their movement into the follicle and disappearance [21,22]. Early studies of SSMs in rodents highlighted their ability to acquire various antigens including ferritin, colloidal carbon, liposomes and opsonized antigens. Comparisons of unimmunized and passively immunized animals showed that opsonization greatly increased antigen capture by SSMs [7,23]. Electron microscopy studies suggested the cells have a low rate of opsonized antigen internalization and degradation [23,24]. Their slower rate of ablation by clodronate loaded liposomes (CLLs) than medullary macrophages supports this view [10]. Further support for the idea that SSMs retain complexes on their surface came from real-time imaging experiments where phycoerythrin immune complexes (PE ICs) appeared to move along the surface of cell processes into follicles [20]. However, the resolution of these imaging experiments did not rule out the possibility that complexes are taken into early endosomes and recycled to the cell surface [25].
SSMs were suggested in early studies to be involved in antigen capture and transfer to B cell follicles [23]. This process was directly visualized using 2-photon microscopy [1719]. SSMs were able to capture lymph borne viruses, antigen-loaded 200nm beads, and immune complexes via their ‘heads’ and to translocate and display these particles on their ‘tails’ for encounter by follicular B cells. Follicular B cells migrate through the dense mat of tail processes as they conduct their ‘random walk’ through the follicle, often making close contact with the macrophages. Cognate B cells acquired antigen directly from the macrophages via their B cell receptor [1719]. In the case of opsonized antigens, non-cognate B cells also acquired them via their complement receptors (CR1 and CR2) and facilitated their delivery to follicular dendritic cells [18,20]. The complexes often appeared to be in clumps on the SSM tails and could be acquired in large units by B cells. Whether additional macrophage molecules contribute to the interaction between the B cells and macrophages during antigen capture or transfer is not known. However, it is notable that SSMs express ICAM1 and VCAM1 [19], molecules that can facilitate integrin-enhancement of antigen gathering by cognate B cells [26]. It is also notable that lymphocytes can adhere to SSMs in tissue sections [27] hinting at the existence of additional surface molecule interactions between SSMs and lymphocytes.
Although the capacity of the SSM to capture a variety of lymph-borne antigens is well established, the receptors involved are less clear perhaps because there is often redundancy in the requirements for binding macromolecular complexes. SSMs express complement receptor 3 (Cr3) [19,20,28], and this receptor likely participates in the capture of opsonized antigens, though loss of this receptor-ligand system alone may not prevent their capture ([19] and unpubl. obs.). CD169 can bind appropriately sialylated antigens, and CD169 expressed by SSMs is also a ligand for the mannose receptor (MR) [12,13]. MR can be released in soluble form and may facilitate SSM binding of mannose-rich antigens [29]. Perhaps consistent with endogenous capture of soluble MR, some reports have described variable MR staining on SSMs [5,30,31] despite the absence of detectable mRNA expression by in situ hybridization [32]. Chicken egg-grown influenza virus bound to SSMs in a mannose-binding lectin (MBL) dependent manner [33]. Since MBL activates the complement cascade, this binding could involve complement receptors but might also indicate the presence of MBL receptors.
In an effort to study the cell biology of these unusual macrophages, several groups have attempted their isolation by LN digestion and cell sorting [19,20,3437]. Cells that were CD11bhiCD169hiF4/80CD11clo were identified and studied [20]. When taken from mice that had been immunized with PE ICs these cells were PE-labeled and perturbations that increased or decreased the number of SSMs in sections caused a corresponding increase or decrease in CD11bhiCD169hiF4/80CD11clo cell frequencies in LN cell suspensions [20]. However, in continued efforts to characterize these cells, we have found that the purity of this population can be variable and sometimes very low; instead the majority of cells staining for these markers are often lymphocytes that have acquired large macrophage-derived blebs (Fig. 2 and unpubl. obs.). These blebs carry all the markers associated with the SSMs, thus causing the attached cells to masquerade as SSMs during flow cytometric analysis. The lymphocytes capturing the blebs are not a random sampling of LN lymphocytes but are enriched for CD4 and CD8 double-negative, IL7Rαhi T cells and NK cells, and in situ assessments indicate that cells of these types are often enriched near CD169+ macrophages [38] (and manuscript in preparation). It seems possible that some of the CD169+CD11chi cells that have been detected by flow cytometry [36] may be DCs that have captured CD169+ macrophage blebs, given the limited evidence for cells expressing high amounts of both markers in tissue sections. We are continuing to investigate whether lymphocytes acquire macrophage blebs in vivo or during the process of tissue digestion and isolation. Perhaps the rigid nature of the macrophage processes or their tight association with the extracellular matrix make them prone to fragmentation during tissue preparation (in contrast to DCs that may quickly retract their mobile cell processes [39] during tissue digestion). Future studies should consider whether bleb capture reflects physiological interactions of lymphocytes with SSMs and what the functional role of this interaction might be. At the time of this writing we suspect that the macrophage gated populations examined in a number of studies may be heavily contaminated by bleb-coated lymphocytes. This may account for the low levels of CD169 transcripts detected in sorted ‘SSMs’ [20][and www.ImmGen.org] and is a consideration that must be kept in mind when analyzing gene expression data generated from such cell preparations. As in past studies that have led to the successful characterization of new cell types, it will be important in future work to routinely confirm the identity of isolated tissue macrophages using microscopy procedures to examine cell morphology and the distribution of key markers over the plasma membrane.
Figure 2
Figure 2
CD169+ macrophage-derived blebs are acquired by lymphocytes
Anatomically, medullary sinuses are irregularly shaped, fluid-containing spaces that are lined by lymphatic endothelial cells and traversed by fine reticular strands. They contain numerous MSMs, which are attached to and sometimes appearing as part of the sinus wall, as well as being attached to reticular fibers within the lumen [4,9,24,30,40,41]. Medullary sinuses also contain lymphocytes that are in the act of egressing the LN and small numbers of plasma cells (Fig. 1). Cortical sinuses connect to medullary sinuses and cells and lymph from the cortical sinuses flow into medullary sinuses [42]. Cortical sinuses are densely packed with egressing lymphocytes and, in contrast to the medullary sinuses, contain few if any macrophages [42]. The factors determining the different properties of these interconnected lymphatic sinuses are not known. The parenchyma between medullary sinuses corresponds to the medullary cords (Fig. 1). These structures are packed with cells, predominantly plasma cells and their precursors, macrophages, some small lymphocytes and DCs, and often contain a blood vessel. The medulla can represent a small part of the LN in the resting state but becomes enlarged at the peak of the plasma cell response with a great increase in the number of short-lived plasma cells. Polymorphonuclear cells enter LNs rapidly following immunization or infection and are often found in significant numbers in LN sinuses [9,4345]. Perhaps the three best-defined functions of the LN medulla are: (1) phagocytosis and clearance of pathogens and particulate antigens from the lymph; (2) supporting short-lived plasma cell survival; (3) providing a traffic path for egressing cells (and antibody) to reach the efferent lymph.
The most abundant macrophages in the mouse medullary regions share the CD169 and Mac1 markers with SSMs but are also F4/80+ [20,46]; many are also SIGNR1+ [47,48], MARCO+ [47,49], MR+ [5,3032] and LYVE1+ [50,51]. Based on their abundance in stained sections, we presume cells with these marker properties are predominantly MSMs (Fig. 1), though studies that combine detection of these markers with retention of the medullary sinus structure are needed to properly address this point. Studies of human LNs indicate the SIGNR1 relative, DC-SIGN, and MARCO (as well as LYVE1) can be present on the sinus lining endothelium as well as macrophages, highlighting the importance of performing adequate resolution microscopy to achieve accurate marker assignment [5,41]. Loss of function studies have established that SIGNR1 is the major receptor for bacterial dextrans as well as the capsular pneumococcal polysaccharide of Streptococcus pneumoniae in the spleen and it may also bind C1q [52]. MARCO is a member of the scavenger receptor family and can mediate binding of a range of bacteria [53,54].
In the early hours following subcutaneous injection of labeled antigen, the major site of label accumulation in LNs is the medullary region. Although the sinuses and cords were not always resolved in these studies, the majority of antigen capture presumably occurred by the lymph-exposed MSMs. Based on morphological features and the amounts of labeled antigen they internalized, these cells are classified as highly active phagocytes and they have large lysosomes and heterogeneous collections of vesicles [4,8,9]. In contrast, work in rat LNs indicates that macrophages within the adjacent medullary cords (MCMs) have small lysosomes and homogenous vesicles [9]. This same report showed that MSMs have high acid phosphatase and low non-specific esterase activity whereas MCMs have the reciprocal enzyme activity pattern [9]; although the significance of these differences in enzyme activity is not known, they provide phenotypic evidence that these macrophages are distinct. As another distinction, MSMs are sometimes seen containing apoptotic polymorphonuclear cells such as eosinophils whereas MCMs frequently contain apoptotic plasma cells [9,43]. Substantial plasma cell apoptosis occurs after the peak of the primary immune response with almost 10% of the plasma cells at a given moment on day 11 after immunization being apoptotic [55]. Thus, MCMs may have a specialized ability to efficiently clear these membrane dense cells.
As well as clearing dying plasma cells, a likely non-phagocytic function of MCMs is provision of trophic support to plasmablasts and plasma cells. Examining mouse LNs at day 8 of the response to antigen in alum, Mohr et al., observed CD169F4/80+CD11bhi cells adjacent to plasma cells in the medullary region; although the structure of sinuses and cords were not always distinguishable in these sections, the close association with plasma cells suggests the macrophages were present within cords [37]. The isolated cells had a macrophage morphology using Giemsa-Grünwalt staining and were major producers of APRIL, a plasma cell survival factor [37]. The numbers of these ‘MCMs’ increased markedly by day 8 of the response. Whether these CD169F4/80+ cells correspond to the non-specific esterase high macrophages enriched within medullary cords in the rat needs investigation.
The extent of macrophage communication between medullary sinuses and cords is not clear though some work has suggested MSMs can be part of the sinus wall [41]. Although most particles entering the medullary sinuses are phagocytosed and degraded locally, limited amounts of antigen may enter the cords or reach the paracortex. In a mouse influenza virus vaccination model, labeled virus accumulated in the medullary region of draining LNs and some gained access to DCs in the cords, a pathway that may facilitate antigen presentation to lymphocytes [33]. Soluble protein antigens that are not efficiently captured by phagocytes may diffuse from medullary sinuses into cortical sinuses and gain access to the T zone [42]. T cells reaching LNs via the afferent lymph may also access the T zone via the medullary region [56]. T cells reaching LNs via afferent lymphatics emanating from non-lymphoid tissues are mostly effector or memory type [57] and this migration path may ensure they survey the medullary region for infected cells and are exposed to any locally produced inflammatory cytokines.
In human LNs, the phenotypic distinctions between SSMs and MSMs have not yet been well defined. One study has described CD14 and CD68 expressing macrophages situated in SCS and medullary locations and also distributed in the walls of the trabecular sinuses [5]. Whether these cells also express CD169 is not yet clear though another report noted the presence of CD169 on macrophages situated in a “perifollicular sinusoidal location”, possibly SSMs [58]. In contrast to mice, human SSMs as well as MSMs stained positively for DC-SIGN (CD209), the functional ortholog of SIGNR1 [5,59].
Like macrophages at other sites, medullary macrophages may have a role in taking up and sensing lipids. Mesenteric LNs are exposed to high amounts of triglyceride rich chylomicrons from dietary fat. In Angptl4-deficient mice that have defective regulation of short chain fatty acid generation these LNs undergo dramatic expansion and contain numerous lipid-laden macrophages, including multinucleated F4/80+ giant cells [60]. Whether changes in mesenteric LN macrophage inflammatory state occur during dietary changes in lipid uptake, and how such changes modulate the antigen clearance and immune functions of LNs are unknown.
The areas of LN parenchyma (or cortex) between B cell follicles show an unusually high amount of cellular heterogeneity. SSMs appear frequently to penetrate from the SCS region and to be present in interfollicular regions (Fig. 1). In addition, cells with the marker characteristics of MSMs, including F4/80, SIGNR1 and MARCO can be present, though the uniformity of marker expression may vary [20,47,48]. Although interfollicular regions usually contain some lymphatic sinus structure, it is often unclear whether these are blind ended structures that allow ‘pooling’ of lymph from the SCS or whether they are interconnected to the medullary (or cortical) sinuses. Moreover, in some cases, depending on the orientation of the LN during sectioning and the proximity of the B cell areas to the medulla, the distinction between interfollicular regions and medullary regions can be difficult to discern. Interfollicular areas contain a high frequency of B cells, T cells and DCs and are a major site of DC influx to the LN from the afferent lymph [56]. A further indication of their specialization is the directed movement of activated B cells and T cells into these regions during immune responses [61,62]. In the case of activated B cells, this movement is promoted by EBI2 (GPR183) and its oxysterol ligand 7α,25-dihydroxycholesterol [61,63]. It is possible that the reduced antibody responses observed in EBI2-deficient mice are a consequence of a reduced ability for activated B cells to interact with macrophages in interfollicular regions. Macrophages may also deliver antigens to DCs in this region to foster CD8 T cell responses, as discussed further below. As well as interfollicular regions, LN macrophages are occasionally observed extending more deeply along the B-T boundary or into the T zone [20,36].
LN medullary macrophages are recognized for their ability to capture, phagocytose and destroy pathogens draining from sites of infection, a process that helps prevent systemic spread [64]. Therefore it has come as something of a surprise to learn that SSMs and their CD169+ counterparts in the spleen, MMMs, are prone to infection by a range of viruses [35,6568]. These observations suggest a permissivity of SSMs and MMMs for viral infection [66]. The physiological basis for and benefits of this permissivity are beginning to come to light. In the case of systemic vesicular stomatitis virus (VSV) infection, many blood-exposed macrophage types become rapidly infected. However, viral replication is only detected in the spleen and here only within MMMs [69]. Building from evidence that type I IFN receptor signaling is highly restrictive for VSV replication, Honke et al., hypothesized that removing Usp18, a negative regulator of IFNαR1 signaling, would reduce the extent of viral replication [69]. Consistent with their model, they found that MMMs expressed Usp18 and that viral replication in the spleen was prevented in Usp18−/− mice, suggesting that MMMs, and thus perhaps SSMs, depend on Usp18-mediated negative regulation of IFNR1 to sustain a viral replication permissive state. This specialized property of MMMs and SSMs may account for the apparently selective replication of other viruses within these macrophages: vaccinia virus (VV) [66] and cow pox virus [67] replicate preferentially in SSMs after subcutaneous inoculation; murine cytomegalovirus infected both SSMs in the mediastinal LN and splenic MMMs following peritoneal inoculation [67]; lymphocytic choriomeningitis virus (LCMV) replicated in MMMs (and possibly MZMs) in the spleen after intravenous infection [68]. In addition, dengue virus replicated in SSMs following skin infection [70] though mice lacking IFNαR1 and IFNγR were studied here because of dengue’s ability to inhibit human but not mouse IFNR signaling; given the Usp18 findings it would be interesting to revisit this analysis and ask whether early dengue virus replication can be detected in SSMs of wild-type mice.
The permissivity of SSMs to infection may not be limited to viruses. Subcutaneously inoculated Toxoplasma gondii preferentially infects and replicates within SSMs [45] and IFN-I inhibits T. gondii growth in monocyte-derived macrophages [71]. Whether other properties of SSMs (and MMMs) make them unusually permissive to infection by intracellular pathogens, such as low lysosomal activity or repression of intracellular pathogen sensing pathways remains an interesting area for future investigation. Of course, these cells are unlikely to lack all anti-pathogen defense mechanisms. Indeed, following Leishmania donovani infection, splenic MZMs and MMMs showed IRF7 recruitment to phagosomes and IRF7 deficiency was associated with a small increase in intracellular parasite load [72]. Finally, the expression of CD4 by SSMs in rodents [9][and unpubl. obs.] raises the question of whether CD4 is expressed by human SSMs and, if so, what role these cells play during HIV propagation in human LNs.
Viral replication in SSMs may augment LN immune responses in a number of ways. In the case of subcutaneous VSV infection, viral replication in SSMs was necessary for the production of sufficient IFN-I to protect intranodal nerves from infection [35]. The IFN-I appeared to come both from the SSMs and from plasmacytoid DCs that were recruited into their proximity [35]. Similarly, splenic MMMs and MZMs are the major IFN-I producers following intravenous HSV infection [73]. A second function of permissive viral replication in SSMs may be to generate intact viral particles to provoke antibody responses, since viral particles are more effective in promoting B cell activation than free antigens [74]. In the case of systemic VSV infection, ablation of CD169+ cells by diphtheria toxin (DT) treatment of mice expressing the DT receptor in CD169+ cells (CD169-DTR mice), or removal of Usp18 and thus suppression of viral replication in CD169+ cells, led to a reduced antibody (and T cell) response [69]. However, following subcutaneous VSV infection, macrophage ablation by CLL treatment led to an augmented antibody response [35]. This different outcome might indicate that the less selective CLL-based macrophage-ablation tool led to a more drastic loss of viral clearance capability, thus increasing exposure of adaptive immune cells to virus or virally-induced inflammatory mediators. Future studies of the LN response to VSV in mice in which CD169+ cells have been selectively ablated may help address this discrepancy.
A third benefit of SSM viral replication permissivity may be to facilitate the development of effector CD8 T cell responses, either directly, by presenting antigen to T cells, or indirectly, by antigen transfer to nearby DCs. Following subcutaneous VV infection, 85% of the infected LN cells were CD169+ macrophages, with the majority of these being F4/80− SSMs [75]. Antigen specific CD8 T cells accumulated in proximity to these macrophages in interfollicular regions, but most CD8 T cell contacts were with DCs, and priming was more successful via DCs [66,75]. Presentation by the macrophages alone was able to stimulate some T cell division, but was not good at generating effector CD8 T cells. One factor to keep in mind in this context was the use of DT treatment of CD11c-DTR mice to study the antigen presenting contribution of DCs versus macrophages. Many LN macrophages, as well as splenic MZ macrophages, express sufficient amounts of CD11c to be ablated by DT treatment of CD11c-DTR mice [35,76,77][and unpubl. obs.] and consistent with this, a ~10-fold reduction in VV infected macrophages was noted in the DT treated CD11c-DTR mice [75]. Thus, the functional properties being studied following DT treatment are of the toxin-resistant fraction of macrophages.
Perhaps the replication of virus in CD169+ macrophages permits subsequent infection of nearby DCs, facilitating their full activation, recruitment of CD8 T cells and antigen presentation. Further evidence for antigen transfer from sinus associated macrophages to T cell-priming DCs comes from the finding that antibody-mediated targeting of antigen to CD169+ MMMs in the spleen led to cross-presentation by CD8+ DCs to achieve effective CTL activation [77]. Similarly, CD169+ macrophages participated in apoptotic cell capture in the spleen but presentation to T cells was by neighboring CD8+CD103+ DCs [78]. SSMs in LNs captured subcutaneously injected apoptotic cells and when SSMs and other CD169+ cells were ablated by treating CD169-DTR mice with DT, CD8 T cell priming against an apoptotic cell antigen failed [36]. CD11chi cells were involved in antigen presentation to the CD8 T cells, and we suspect that these cells acquired apoptotic cell-derived antigen from SSMs or CD169+ interfollicular macrophages [36]. This study suggests a further mechanism by which SSMs might contribute to anti-viral CD8 T cell responses is by capturing apoptotic cells or cell debris draining to lymph nodes from infected tissues and making viral antigen available for the subsequent priming of CD8 T cell responses.
During the splenic response to LCMV there was marked upregulation of CXCR3 ligands in the MZ, likely made by MMMs, and CXCR3-mediated guidance of CD8 T cells to the MZ was critical for generation of short-term effector cells [68]. These data suggested that recruitment of cognate T cells into the region of high viral antigen density is important for generating short-term effector cells. Although the study did not determine whether the macrophages were directly involved in antigen presentation to the CD8 T cells or whether a DC intermediary was involved, it seems likely that the CXCR3 receptor-ligand system will operate along with the CCR5 receptor-ligand system [75] during LN macrophage infections that result in effector CD8 T cell recruitment and activation.
The ability of infected SSMs to present antigens in the context of MHC class I is indicated by their ability to be killed by CD8 cells during Toxoplasma infection [79]. However, whether they express sufficient amounts of costimulatory molecules to support naïve T cell priming is less clear, and most studies to date suggest they are not well equipped for this function, though they are apparently competent to promote activation of the effector type iNKT cell [80].
The ability of SSMs to present CD1d-restricted lipid antigens to iNKT cells was tested by immunizing mice with 200nm silica microspheres coated with αGalCer containing liposomes [80]. These particles colocalized with CD169 staining cells in mediastinal LNs 2 hr after intraperitoneal immunization and real-time imaging showed that iNKT cells made increased numbers of contacts with SSMs 6 hr after antigen administration. Depletion of macrophages (but not DCs or B cells) using CLLs led to a loss of iNKT cell activation. Given that lipid loading on CD1d is thought to require passage through endocytic compartments, these data suggest that SSMs internalized some of the lipid. However, whether the cells actively phagocytose the 200nm microspheres or instead absorb some of the surface displayed liposomes remains to be assessed. Recent evidence has highlighted the importance of CD1d presentation of the endogenous lipid βGlcCer and provides evidence that upregulation of lipid presentation occurs following exposure to innate stimuli such as LPS [81]. Given the ability of SSMs to bind lymph borne LPS [82], endogenous lipid presentation may be another pathway of SSM-mediated iNKT cell activation.
To fully understand LN macrophage heterogeneity and to be able to genetically probe LN macrophage functions it is necessary to characterize their development. The developmental pathways of different tissue macrophage types are only now beginning to be elucidated [3]. In mice lacking CSF1 (op/op mice) there is a loss of CD169+ SSMs (as well as splenic MMMs and MZMs) whereas medullary F4/80+ cells are retained [83,84]. The CSF1-dependence of SSMs confirms their macrophage identity [3]. A second key cytokine requirement for SSMs (as well as splenic MMMs and MZMs) is LTα1β2 [20,85,86]. Although mice lacking this TNF family member or its receptor, LTβR, lack most or all LNs the role of LTβR signaling in LNs can be studied by treating mice with the soluble antagonist, LTβR-Ig [87]. Bockade of LTβR signaling for 4 weeks caused a marked decrease in CD169+F4/80− cell abundance in LN sections and a reduction in CD169-staining cells detected by flow cytometry [20]. While the latter analysis suffers from contamination of the gated populations by macrophage-bleb coated lymphocytes, it appears that the number of bleb coated cells decreases in proportion with the decrease in macrophage frequency. B cells were a necessary source of LTα1β2 for development and/or maintenance of a normal SSM population [20] as they are for MMMs and MZMs [86]. LTβR signals via NFkB transcription factors and hematopoietic cell deficiency in NFkB p52 causes deficiencies in splenic CD169+ macrophages [88]; the role of NFkB transcription factors in LN macrophages has not yet been reported. TNF also contributes to MMM and MZM development/maintenance, though more weakly than LTβR, whereas it may not be required for LN macrophage homeostasis [89]. LTα1β2 levels in LNs under homeostatic conditions are limiting for SSM induction or maintenance as mice that transgenically overexpress LTα1β2on B cells have a thicker layer of SSMs and greater frequencies of CD169-staining cells in flow cytometry [20]. It seems likely that the LTα1β2-dependence of SSMs serves as a mechanism to achieve homeostasis between the size of the B cell follicle, which can grow rapidly during immune responses, and the surface coverage by SSMs. Following ablation by CLL treatment, SSMs and MSMs took several months to be replenished [10]. However, when the cells were more selectively ablated by DT treatment of CD169-DTR mice, there was substantial reconstitution within 10 days [36]. Similarly, when MMMs were ablated by DT treatment of CD11c-DTR or CD169-DTR mice, they reappeared in significant numbers by day 10 [76,78]. It seems possible that the slow rate of SSM replacement in mice treated with CLL reflects a long-lasting effect of this macrophage toxin. CD169+ LN macrophages were replaced efficiently (>80%) by bone marrow-derived cells in radiation chimeras, confirming their bone marrow origin [20].
The requirements for MSM and MCM development are less well understood than for SSMs. F4/80+ macrophages were retained in the medullary region of mice following a month of LT-blockade [20] and CSF1-deficient mice also retained F4/80+ cells [83,84]. Whether the F4/80+CD169+ (‘MSM’) and F4/80+CD169− (‘MCM’) subsets were equally retained has not yet been investigated. Recent studies have indicated that CSF1R deficiency causes a more penetrant loss of some macrophage types than occurs in CSF1-deficient mice, the difference likely reflecting the activity of IL34 as a second CSF1R ligand [3,90]. It will be important to determine whether medullary macrophages are more dependent on CSF1R than CSF1. Such studies combined with lineage tracing experiments should help determine whether each LN macrophage type differentiates from a common precursor, perhaps with medullary macrophages giving rise to SSMs in a LT-and CSF1-dependent manner, or whether the cells have distinct precursors.
As noted above, there are a number of similarities between LN SSMs and splenic MMMs: both are CD169hi, depend on CSF1, B cells and LTα1β2, are prone to viral infection and are situated at the interface of the follicle and sinus. However, there are also notable distinctions, such as the ready capture of immune complexes by SSMs but not MMMs. This may reflect anatomical differences of the tissues but could also relate to intrinsic differences in these cells that match their functions with the types of pathogens and antigens they typically encounter. In a similar way, LN MSMs and splenic MZMs share markers such as SIGNR1 and MARCO and the property of being highly phagocytic, but important differences exist such as higher CD169 expression on MSMs and lack of F4/80 expression on MZMs. The parallels between LN MCMs and macrophages in the spleen are less clear at present but given the abundant F4/80 expression in the splenic red pulp and the role of this compartment in supporting short-lived plasma cells, it seems likely that there will be an MCM-related subset of red pulp macrophages. We anticipate that the related subsets of LN and splenic macrophages will share much of their developmental program but that differences in the final maturation signals will specify fates unique to their tissue of residence.
Recent studies in rodents have led to advances in the understanding of SSMs, including their function in antigen capture, translocation and display to B cells, their permissivity to pathogen replication and their roles in CD8 T cell and iNKT cell responses. Advances in our understanding of medullary macrophages have been more limited. The highly phagocytic MSMs mediate clearance of particulates, pathogens and dying cells from the lymph, while MCMs may support plasma cell survival and clear away their dead. A key challenge in further defining the differences between medullary sinus and medullary cord macrophages will be to maintain the sinus spaces intact to allow cells within sinuses and cords to be more routinely distinguished. This should in turn facilitate improved mapping of surface markers and functional properties onto MSMs and MCMs. In humans, a more detailed comparison of SSMs and MSMs is also needed to determine whether these subsets have distinct properties, similar to their rodent counterparts. Generation of genetic tools that allow ablation of individual LN macrophage subsets will also help advance the field. Further work is needed to understand the macrophage complexity in interfollicular regions where SSMs, MSMs and perhaps additional macrophage types comingle and crosstalk with DCs and lymphocytes.
Sinus lining lymphatic endothelium in LNs expresses endocytic receptors [41,91] and can take up a variety of materials, sometimes in large amounts. For example, silica-coated iron oxide nanoparticles were preferentially taken up by LYVE1+ SCS lining cells rather than macrophages [92] and viral particles have been found in medullary sinus lining cells [19]. Future work needs to explore how sinus lining endothelial cells and the closely associated macrophages interact and share their antigen handling and barrier functions.
While this review has attempted to emphasize general themes in LN macrophage biology, it is likely that important differences exist between macrophages in LNs draining different tissue beds. One indication of this is provided by the lipid accumulation in mesenteric LN macrophages of angptl4-deficient mice. Another may be the ‘firewall’ function of mesenteric LNs in preventing commensal bacteria that breach the intestinal epithelial barrier from reaching systemic circulation [93]. We can anticipate that just as stromal cells, endothelial cells and DCs from peripheral and mucosal LNs differ, so will key aspects of macrophage phenotype and function. This may be as a consequence of different factors flowing to the LN in the lymph but may also reflect local morphogenic differences within the LN. Moreover, although macrophages don’t have the remarkable migratory behavior of DCs, there are some conditions such as during solid tumor growth where considerable numbers of tissue macrophages may reach and modify draining LNs.
To properly characterize LN macrophages, and their relatives in the spleen, improved procedures for their isolation to high purity are needed. Methods that allow their culture will also greatly enhance their study. New insights into their biology are also likely to emerge from a more complete assessment of their developmental pathway(s) and relationships to other macrophage populations in the body.
Acknowledgments
We thank Tri Phan and Cliff Lowell for helpful discussions and comments on the manuscript. Work from the Cyster laboratory discussed in this review was supported in part by NIH grant AI45073.
1. Gordon S, Taylor PR. Monocyte and macrophage heterogeneity. Nat Rev Immunol. 2005;5:953–964. [PubMed]
2. Gordon S. The macrophage: Past, present and future. Eur J Immunol. 2007;37 (Suppl 1):S9–17. [PubMed]
3. Hashimoto D, Miller J, Merad M. Dendritic cell and macrophage heterogeneity in vivo. Immunity. 2011;35:323–335. [PubMed]
4. Young B, Heath JW. Wheater’s functional histology. 4. Churchill Livingstone; 2000.
5. Angel CE, Chen CJ, Horlacher OC, Winkler S, John T, Browning J, MacGregor D, Cebon J, Dunbar PR. Distinctive localization of antigen-presenting cells in human lymph nodes. Blood. 2009;113:1257–1267. [PubMed]
6. Clark SL. The reticulum of lymph nodes in mice studied with the electron microscope. Am J Anat. 1962;110:217–257. [PubMed]
7. Nossal GJ, Ada GL, Austin CM, Pye J. Antigens in immunity. 8. Localization of 125-i-labelled antigens in the secondary response. Immunology. 1965;9:349–357. [PubMed]
8. Fossum S. The architecture of rat lymph nodes. Iv. Distribution of ferritin and colloidal carbon in the draining lymph nodes after foot-pad injection. Scand J Immunol. 1980;12:433–441. [PubMed]
9. Steer HW, Foot RA. Changes in the medulla of the parathymic lymph nodes of the rat during acute gastro-intestinal inflammation. J Anat. 1987;152:23–36. [PubMed]
10. Delemarre FG, Kors N, Kraal G, van Rooijen N. Repopulation of macrophages in popliteal lymph nodes of mice after liposome-mediated depletion. J Leukoc Biol. 1990;47:251–257. [PubMed]
11. Kraal G, Mebius R. New insights into the cell biology of the marginal zone of the spleen. Int Rev Cytol. 2006;250:175–215. [PubMed]
12. Martinez-Pomares L, Gordon S. Cd169(+) macrophages at the crossroads of antigen presentation. Trends Immunol. 2011 [PubMed]
13. Crocker PR, Paulson JC, Varki A. Siglecs and their roles in the immune system. Nat Rev Immunol. 2007;7:255–266. [PubMed]
14. Kumamoto Y, Higashi N, Denda-Nagai K, Tsuiji M, Sato K, Crocker PR, Irimura T. Identification of sialoadhesin as a dominant lymph node counter-receptor for mouse macrophage galactose-type c-type lectin 1. J Biol Chem. 2004;279:49274–49280. [PubMed]
15. Gordon S, Hamann J, Lin HH, Stacey M. F4/80 and the related adhesion-gpcrs. Eur J Immunol. 2011;41:2472–2476. [PubMed]
16. Farr AG, Cho Y, De Bruyn PP. The structure of the sinus wall of the lymph node relative to its endocytic properties and transmural cell passage. Am J Anat. 1980;157:265–284. [PubMed]
17. Carrasco YR, Batista FD. B cells acquire particulate antigen in a macrophage-rich area at the boundary between the follicle and the subcapsular sinus of the lymph node. Immunity. 2007;27:160–171. [PubMed]
18. Phan TG, Grigorova I, Okada T, Cyster JG. Subcapsular encounter and complement-dependent transport of immune complexes by lymph node b cells. Nat Immunol. 2007;8:992–1000. [PubMed]
19. Junt T, Moseman EA, Iannacone M, Massberg S, Lang PA, Boes M, Fink K, Henrickson SE, Shayakhmetov DM, Di Paolo NC, van Rooijen N, Mempel TR, Whelan SP, von Andrian UH. Subcapsular sinus macrophages in lymph nodes clear lymph-borne viruses and present them to antiviral b cells. Nature. 2007;450:110–114. [PubMed]
20. Phan TG, Green JA, Gray EE, Xu Y, Cyster JG. Immune complex relay by subcapsular sinus macrophages and noncognate b cells drives antibody affinity maturation. Nat Immunol. 2009;10:786–793. [PMC free article] [PubMed]
21. Mebius RE, Hendriks HR, Breve J, Kraal G. Macrophages and the activity of high endothelial venules. The effect of interferon-gamma. Eur J Immunol. 1990;20:1615–1618. [PubMed]
22. Mebius RE, Streeter PR, Breve J, Duijvestijn AM, Kraal G. The influence of afferent lymphatic vessel interruption on vascular addressin expression. J Cell Biol. 1991;115:85–95. [PMC free article] [PubMed]
23. Szakal AK, Holmes KL, Tew JG. Transport of immune complexes from the subcapsular sinus to lymph node follicles on the surface of nonphagocytic cells, including cells with dendritic morphology. J Immunol. 1983;131:1714–1727. [PubMed]
24. Fossum S, Vaaland JL. The architecture of rat lymph nodes. I. Combined light and electron microscopy of lymph node cell types. Anat Embryol (Berl) 1983;167:229–246. [PubMed]
25. Cyster JG. B cell follicles and antigen encounters of the third kind. Nat Immunol. 2010;11:989–996. [PubMed]
26. Fleire SJ, Goldman JP, Carrasco YR, Weber M, Bray D, Batista FD. B cell ligand discrimination through a spreading and contraction response. Science. 2006;312:738–741. [PubMed]
27. van den Berg TK, Breve JJ, Damoiseaux JG, Dopp EA, Kelm S, Crocker PR, Dijkstra CD, Kraal G. Sialoadhesin on macrophages: Its identification as a lymphocyte adhesion molecule. J Exp Med. 1992;176:647–655. [PMC free article] [PubMed]
28. Witmer MD, Steinman RM. The anatomy of peripheral lymphoid organs with emphasis on accessory cells: Light-microscopic immunocytochemical studies of mouse spleen, lymph node, and peyer’s patch. Am J Anat. 1984;170:465–481. [PubMed]
29. Taylor PR, Zamze S, Stillion RJ, Wong SY, Gordon S, Martinez-Pomares L. Development of a specific system for targeting protein to metallophilic macrophages. Proc Natl Acad Sci U S A. 2004;101:1963–1968. [PubMed]
30. Takahashi K, Donovan MJ, Rogers RA, Ezekowitz RA. Distribution of murine mannose receptor expression from early embryogenesis through to adulthood. Cell Tissue Res. 1998;292:311–323. [PubMed]
31. McKenzie EJ, Taylor PR, Stillion RJ, Lucas AD, Harris J, Gordon S, Martinez-Pomares L. Mannose receptor expression and function define a new population of murine dendritic cells. J Immunol. 2007;178:4975–4983. [PubMed]
32. Linehan SA, Martinez-Pomares L, Stahl PD, Gordon S. Mannose receptor and its putative ligands in normal murine lymphoid and nonlymphoid organs: In situ expression of mannose receptor by selected macrophages, endothelial cells, perivascular microglia, and mesangial cells, but not dendritic cells. J Exp Med. 1999;189:1961–1972. [PMC free article] [PubMed]
33. Gonzalez SF, Lukacs-Kornek V, Kuligowski MP, Pitcher LA, Degn SE, Kim YA, Cloninger MJ, Martinez-Pomares L, Gordon S, Turley SJ, Carroll MC. Capture of influenza by medullary dendritic cells via sign-r1 is essential for humoral immunity in draining lymph nodes. Nat Immunol. 2010;11:427–434. [PMC free article] [PubMed]
34. Barral P, Polzella P, Bruckbauer A, van Rooijen N, Besra GS, Cerundolo V, Batista FD. Cd169(+) macrophages present lipid antigens to mediate early activation of inkt cells in lymph nodes. Nat Immunol. 2010;11:303–312. [PMC free article] [PubMed]
35. Iannacone M, Moseman EA, Tonti E, Bosurgi L, Junt T, Henrickson SE, Whelan SP, Guidotti LG, von Andrian UH. Subcapsular sinus macrophages prevent cns invasion on peripheral infection with a neurotropic virus. Nature. 2010;465:1079–1083. [PMC free article] [PubMed]
36. Asano K, Nabeyama A, Miyake Y, Qiu CH, Kurita A, Tomura M, Kanagawa O, Fujii SI, Tanaka M. Cd169-positive macrophages dominate antitumor immunity by crosspresenting dead cell-associated antigens. Immunity. 2010;34:85–95. [PubMed]
37. Mohr E, Serre K, Manz RA, Cunningham AF, Khan M, Hardie DL, Bird R, MacLennan IC. Dendritic cells and monocyte/macrophages that create the il-6/april-rich lymph node microenvironments where plasmablasts mature. J Immunol. 2009;182:2113–2123. [PubMed]
38. Gray EE, Suzuki K, Cyster JG. Cutting edge: Identification of a motile il-17-producing {gamma}{delta} t cell population in the dermis. J Immunol. 2011 [PMC free article] [PubMed]
39. Miller MJ, Hejazi AS, Wei SH, Cahalan MD, Parker I. T cell repertoire scanning is promoted by dynamic dendritic cell behavior and random t cell motility in the lymph node. Proc Natl Acad Sci U S A. 2004;101:998–1003. [PubMed]
40. Fossum S. The architecture of rat lymph nodes. Ii. Lymph node compartments. Scand J Immunol. 1980;12:411–420. [PubMed]
41. Martens JH, Kzhyshkowska J, Falkowski-Hansen M, Schledzewski K, Gratchev A, Mansmann U, Schmuttermaier C, Dippel E, Koenen W, Riedel F, Sankala M, Tryggvason K, Kobzik L, Moldenhauer G, Arnold B, Goerdt S. Differential expression of a gene signature for scavenger/lectin receptors by endothelial cells and macrophages in human lymph node sinuses, the primary sites of regional metastasis. J Pathol. 2006;208:574–589. [PubMed]
42. Grigorova IL, Panteleev M, Cyster JG. Lymph node cortical sinus organization and relationship to lymphocyte egress dynamics and antigen exposure. Proc Natl Acad Sci U S A. 2010;107:20447–20452. [PubMed]
43. Nossal GJ, Abbot A, Mitchell J. Antigens in immunity. Xiv. Electron microscopic radioautographic studies of antigen capture in the lymph node medulla. J Exp Med. 1968;127:263–276. [PMC free article] [PubMed]
44. Yang CW, Strong BS, Miller MJ, Unanue ER. Neutrophils influence the level of antigen presentation during the immune response to protein antigens in adjuvants. J Immunol. 2010;185:2927–2934. [PMC free article] [PubMed]
45. Chtanova T, Schaeffer M, Han SJ, van Dooren GG, Nollmann M, Herzmark P, Chan SW, Satija H, Camfield K, Aaron H, Striepen B, Robey EA. Dynamics of neutrophil migration in lymph nodes during infection. Immunity. 2008;29:487–496. [PMC free article] [PubMed]
46. Hume DA, Robinson AP, MacPherson GG, Gordon S. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen f4/80. Relationship between macrophages, langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J Exp Med. 1983;158:1522–1536. [PMC free article] [PubMed]
47. Kang YS, Yamazaki S, Iyoda T, Pack M, Bruening SA, Kim JY, Takahara K, Inaba K, Steinman RM, Park CG. Sign-r1, a novel c-type lectin expressed by marginal zone macrophages in spleen, mediates uptake of the polysaccharide dextran. Int Immunol. 2003;15:177–186. [PubMed]
48. Geijtenbeek TB, Groot PC, Nolte MA, van Vliet SJ, Gangaram-Panday ST, van Duijnhoven GC, Kraal G, van Oosterhout AJ, van Kooyk Y. Marginal zone macrophages express a murine homologue of dc-sign that captures blood-borne antigens in vivo. Blood. 2002;100:2908–2916. [PubMed]
49. Elomaa O, Kangas M, Sahlberg C, Tuukkanen J, Sormunen R, Liakka A, Thesleff I, Kraal G, Tryggvason K. Cloning of a novel bacteria-binding receptor structurally related to scavenger receptors and expressed in a subset of macrophages. Cell. 1995;80:603–609. [PubMed]
50. Schledzewski K, Falkowski M, Moldenhauer G, Metharom P, Kzhyshkowska J, Ganss R, Demory A, Falkowska-Hansen B, Kurzen H, Ugurel S, Geginat G, Arnold B, Goerdt S. Lymphatic endothelium-specific hyaluronan receptor lyve-1 is expressed by stabilin-1+, f4/80+, cd11b+ macrophages in malignant tumours and wound healing tissue in vivo and in bone marrow cultures in vitro: Implications for the assessment of lymphangiogenesis. J Pathol. 2006;209:67–77. [PubMed]
51. Grigorova IL, Schwab SR, Phan TG, Pham TH, Okada T, Cyster JG. Cortical sinus probing, s1p1-dependent entry and flow-based capture of egressing t cells. Nat Immunol. 2009;10:58–65. [PMC free article] [PubMed]
52. Kang YS, Do Y, Lee HK, Park SH, Cheong C, Lynch RM, Loeffler JM, Steinman RM, Park CG. A dominant complement fixation pathway for pneumococcal polysaccharides initiated by sign-r1 interacting with c1q. Cell. 2006;125:47–58. [PubMed]
53. van der Laan LJ, Dopp EA, Haworth R, Pikkarainen T, Kangas M, Elomaa O, Dijkstra CD, Gordon S, Tryggvason K, Kraal G. Regulation and functional involvement of macrophage scavenger receptor marco in clearance of bacteria in vivo. J Immunol. 1999;162:939–947. [PubMed]
54. Areschoug T, Gordon S. Scavenger receptors: Role in innate immunity and microbial pathogenesis. Cell Microbiol. 2009;11:1160–1169. [PubMed]
55. Smith KGC, Hewitson TD, Nossal GJV, Tarlinton DM. The phenotype and fate of the antibody-forming cells of the splenic foci. Eur J Immunol. 1996;26:444–448. [PubMed]
56. Braun A, Worbs T, Moschovakis GL, Halle S, Hoffmann K, Bolter J, Munk A, Forster R. Afferent lymph-derived t cells and dcs use different chemokine receptor ccr7-dependent routes for entry into the lymph node and intranodal migration. Nat Immunol. 2011;12:879–887. [PubMed]
57. Mackay CR, Marston WL, Dudler L. Naive and memory t cells show distinct pathways of lymphocyte recirculation. J Exp Med. 1990;171:801–817. [PMC free article] [PubMed]
58. Hartnell A, Steel J, Turley H, Jones M, Jackson DG, Crocker PR. Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations. Blood. 2001;97:288–296. [PubMed]
59. Granelli-Piperno A, Pritsker A, Pack M, Shimeliovich I, Arrighi JF, Park CG, Trumpfheller C, Piguet V, Moran TM, Steinman RM. Dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin/cd209 is abundant on macrophages in the normal human lymph node and is not required for dendritic cell stimulation of the mixed leukocyte reaction. J Immunol. 2005;175:4265–4273. [PMC free article] [PubMed]
60. Lichtenstein L, Mattijssen F, de Wit NJ, Georgiadi A, Hooiveld GJ, van der Meer R, He Y, Qi L, Koster A, Tamsma JT, Tan NS, Muller M, Kersten S. Angptl4 protects against severe proinflammatory effects of saturated fat by inhibiting fatty acid uptake into mesenteric lymph node macrophages. Cell Metab. 2010;12:580–592. [PMC free article] [PubMed]
61. Pereira JP, Kelly LM, Cyster JG. Finding the right niche: B cell migration in the early phases of t-dependent antibody responses. Int Immunol. 2010;22:413–419. [PMC free article] [PubMed]
62. Kerfoot SM, Yaari G, Patel JR, Johnson KL, Gonzalez DG, Kleinstein SH, Haberman AM. Germinal center b cell and t follicular helper cell development initiates in the interfollicular zone. Immunity. 2011;34:947–960. [PMC free article] [PubMed]
63. Hannedouche S, Zhang J, Yi T, Shen W, Nguyen D, Pereira JP, Guerini D, Gaumgarten BU, Roog S, Wen B, Knochenmuss R, Moel S, Gessier F, Kelly LM, Vanek M, Laurent S, Preuss I, Miault C, Christen I, Karuna R, Li W, Koo D-I, Suply T, Schmedt C, Peters EC, Flachetto R, Kataopodis A, Spanka C, Roy M-O, Detheux M, Chen YA, Schultz PG, Cho CY, Seuwen K, Cyster JG, Sailer AW. Oxysterols direct immune cell migration through ebi2. Nature. 2011;475:524–527. [PubMed]
64. Phan TG, Gray EE, Cyster JG. The microanatomy of b cell activation. Curr Opin Immunol. 2009;21:258–265. [PubMed]
65. Norbury CC, Malide D, Gibbs JS, Bennink JR, Yewdell JW. Visualizing priming of virus-specific cd8+ t cells by infected dendritic cells in vivo. Nat Immunol. 2002;3:265–271. [PubMed]
66. Hickman HD, Takeda K, Skon CN, Murray FR, Hensley SE, Loomis J, Barber GN, Bennink JR, Yewdell JW. Direct priming of antiviral cd8+ t cells in the peripheral interfollicular region of lymph nodes. Nat Immunol. 2008;9:155–165. [PubMed]
67. Hsu KM, Pratt JR, Akers WJ, Achilefu SI, Yokoyama WM. Murine cytomegalovirus displays selective infection of cells within hours after systemic administration. J Gen Virol. 2009;90:33–43. [PMC free article] [PubMed]
68. Hu JK, Kagari T, Clingan JM, Matloubian M. Expression of chemokine receptor cxcr3 on t cells affects the balance between effector and memory cd8 t-cell generation. Proc Natl Acad Sci U S A. 2011;108:E118–127. [PubMed]
69. Honke N, Shaabani N, Cadeddu G, Sorg UR, Zhang DE, Trilling M, Klingel K, Sauter M, Kandolf R, Gailus N, van Rooijen N, Burkart C, Baldus SE, Grusdat M, Lohning M, Hengel H, Pfeffer K, Tanaka M, Haussinger D, Recher M, Lang PA, Lang KS. Enforced viral replication activates adaptive immunity and is essential for the control of a cytopathic virus. Nat Immunol. 2011;13:51–57. [PubMed]
70. Fink K, Ng C, Nkenfou C, Vasudevan SG, van Rooijen N, Schul W. Depletion of macrophages in mice results in higher dengue virus titers and highlights the role of macrophages for virus control. Eur J Immunol. 2009;39:2809–2821. [PubMed]
71. Schmitz JL, Carlin JM, Borden EC, Byrne GI. Beta interferon inhibits toxoplasma gondii growth in human monocyte-derived macrophages. Infect Immun. 1989;57:3254–3256. [PMC free article] [PubMed]
72. Phillips R, Svensson M, Aziz N, Maroof A, Brown N, Beattie L, Signoret N, Kaye PM. Innate killing of leishmania donovani by macrophages of the splenic marginal zone requires irf-7. PLoS Pathog. 2010;6:e1000813. [PMC free article] [PubMed]
73. Eloranta ML, Alm GV. Splenic marginal metallophilic macrophages and marginal zone macrophages are the major interferon-alpha/beta producers in mice upon intravenous challenge with herpes simplex virus. Scand J Immunol. 1999;49:391–394. [PubMed]
74. Bachmann MF, Rohrer UH, Kundig TM, Burki K, Hengartner H, Zinkernagel RM. The influence of antigen organization on b cell responsiveness. Science. 1993;262:1448–1451. [PubMed]
75. Hickman HD, Li L, Reynoso GV, Rubin EJ, Skon CN, Mays JW, Gibbs J, Schwartz O, Bennink JR, Yewdell JW. Chemokines control naive cd8+ t cell selection of optimal lymph node antigen presenting cells. J Exp Med. 2011;208:2511–2524. [PMC free article] [PubMed]
76. Probst HC, Tschannen K, Odermatt B, Schwendener R, Zinkernagel RM, Van Den Broek M. Histological analysis of cd11c-dtr/gfp mice after in vivo depletion of dendritic cells. Clin Exp Immunol. 2005;141:398–404. [PubMed]
77. Backer R, Schwandt T, Greuter M, Oosting M, Jungerkes F, Tuting T, Boon L, O’Toole T, Kraal G, Limmer A, den Haan JM. Effective collaboration between marginal metallophilic macrophages and cd8+ dendritic cells in the generation of cytotoxic t cells. Proc Natl Acad Sci U S A. 2010;107:216–221. [PubMed]
78. Miyake Y, Asano K, Kaise H, Uemura M, Nakayama M, Tanaka M. Critical role of macrophages in the marginal zone in the suppression of immune responses to apoptotic cell-associated antigens. J Clin Invest. 2007;117:2268–2278. [PMC free article] [PubMed]
79. Chtanova T, Han SJ, Schaeffer M, van Dooren GG, Herzmark P, Striepen B, Robey EA. Dynamics of t cell, antigen-presenting cell, and pathogen interactions during recall responses in the lymph node. Immunity. 2009;31:342–355. [PubMed]
80. Barral P, Eckl-Dorna J, Harwood NE, De Santo C, Salio M, Illarionov P, Besra GS, Cerundolo V, Batista FD. B cell receptor-mediated uptake of cd1d-restricted antigen augments antibody responses by recruiting invariant nkt cell help in vivo. Proc Natl Acad Sci U S A. 2008;105:8345–8350. [PubMed]
81. Brennan PJ, Tatituri RV, Brigl M, Kim EY, Tuli A, Sanderson JP, Gadola SD, Hsu FF, Besra GS, Brenner MB. Invariant natural killer t cells recognize lipid self antigen induced by microbial danger signals. Nat Immunol. 2011;12:1202–1211. [PMC free article] [PubMed]
82. Lu M, Munford RS. The transport and inactivation kinetics of bacterial lipopolysaccharide influence its immunological potency in vivo. J Immunol. 2011;187:3314–3320. [PMC free article] [PubMed]
83. Witmer-Pack MD, Hughes DA, Schuler G, Lawson L, McWilliam A, Inaba K, Steinman RM, Gordon S. Identification of macrophages and dendritic cells in the osteopetrotic op/op mouse. J Cell Sci. 1993;104(Pt 4):1021–1029. [PubMed]
84. Cecchini MG, Dominguez MG, Mocci S, Wetterwald A, Felix R, Fleisch H, Chisholm O, Hofstetter W, Pollard JW, Stanley ER. Role of colony stimulating factor-1 in the establishment and regulation of tissue macrophages during postnatal development of the mouse. Development. 1994;120:1357–1372. [PubMed]
85. Rennert PD, Browning JL, Mebius R, Mackay F, Hochman PS. Surface lymphotoxin alpha/beta complex is required for the development of peripheral lymphoid organs. J Exp Med. 1996;184:1999–2006. [PMC free article] [PubMed]
86. Tumanov AV, Grivennikov SI, Shakhov AN, Rybtsov SA, Koroleva EP, Takeda J, Nedospasov SA, Kuprash DV. Dissecting the role of lymphotoxin in lymphoid organs by conditional targeting. Immunol Rev. 2003;195:106–116. [PubMed]
87. Browning JL. Inhibition of the lymphotoxin pathway as a therapy for autoimmune disease. Immunol Rev. 2008;223:202–220. [PubMed]
88. Poljak L, Carlson L, Cunningham K, Kosco-Vilbois MH, Siebenlist U. Distinct activities of p52/nf-kappa b required for proper secondary lymphoid organ microarchitecture: Functions enhanced by bcl-3. J Immunol. 1999;163:6581–6588. [PubMed]
89. Pasparakis M, Kousteni S, Peschon J, Kollias G. Tumor necrosis factor and the p55tnf receptor are required for optimal development of the marginal sinus and for migration of follicular dendritic cell precursors into splenic follicles. Cell Immunol. 2000;201:33–41. [PubMed]
90. MacDonald KP, Palmer JS, Cronau S, Seppanen E, Olver S, Raffelt NC, Kuns R, Pettit AR, Clouston A, Wainwright B, Branstetter D, Smith J, Paxton RJ, Cerretti DP, Bonham L, Hill GR, Hume DA. An antibody against the colony-stimulating factor 1 receptor depletes the resident subset of monocytes and tissue- and tumor-associated macrophages but does not inhibit inflammation. Blood. 2010;116:3955–3963. [PubMed]
91. Prevo R, Banerji S, Ni J, Jackson DG. Rapid plasma membrane-endosomal trafficking of the lymph node sinus and high endothelial venule scavenger receptor/homing receptor stabilin-1 (feel-1/clever-1) J Biol Chem. 2004;279:52580–52592. [PubMed]
92. Bumb A, Regino CA, Egen JG, Bernardo M, Dobson PJ, Germain RN, Choyke PL, Brechbiel MW. Trafficking of a dual-modality magnetic resonance and fluorescence imaging superparamagnetic iron oxide-based nanoprobe to lymph nodes. Mol Imaging Biol. 2011;13:1163–1172. [PMC free article] [PubMed]
93. Macpherson AJ, Geuking MB, Slack E, Hapfelmeier S, McCoy KD. The habitat, double life, citizenship, and forgetfulness of iga. Immulogical Reviews. 2011;245:132–146. [PubMed]