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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Infect Control Hosp Epidemiol. Author manuscript; available in PMC 2013 February 13.
Published in final edited form as:
Infect Control Hosp Epidemiol. 2009 April; 30(4): 392–395.
doi:  10.1086/596606
PMCID: PMC3571094

Evaluation of the Effect of Treatment of Latent Tuberculosis Infection on QuantiFERON-TB Gold Assay Results


To evaluate the utility of the QuantiFERON-TB Gold assay for monitoring latent tuberculosis treatment efficacy, the assay was performed serially for healthcare workers receiving isoniazid therapy. After 9 months of isoniazid therapy, all of these healthcare workers remained QuantiFERON-TB Gold positive, and cellular proliferation assays revealed persistently strong purified protein derivative responses. These results do not support the use of the QuantiFERON-TB Gold assay to monitor therapy.

In the United States, the QuantiFERON-TB Gold (QFT-G) assay, an interferon-γ release assay (IGRA), has been advocated for use in tuberculosis (TB) screening programs,1 including those for healthcare workers (HCWs). Standard guidelines2 recommend treatment for HCWs who meet the diagnostic criteria for having latent TB infection (LTBI). However, it is unknown whether IGRAs such as the QFT-G assay can be used as markers of response to therapy for LTBI.

Here, we evaluated whether newly hired HCWs who initially tested positive for Mycobacterium tuberculosis infection with the QFT-G assay on routine screening and who then received a standard, monitored course of LTBI therapy would subsequently test negative with the assay. Therefore, we evaluated serial QFT-G assay results before, during, and 3 months after completion of a 9-month isoniazid treatment course. In parallel, we assessed the effect of treatment on the cellular immune response to purified protein derivative in extended in vitro proliferation assays.


Routine TB screening protocol

The Beth Israel Deaconess Medical Center Employee Occupational Health Services screens approximately 3,000 newly hired employees each year by tuberculin skin test; approximately 15% of these HCWs have positive tuberculin skin test results. QFT-G testing was introduced in 2006 for routine testing of newly hired employees. Baseline tuberculin skin testing was performed in accordance with guidelines of the Beth Israel Deaconess Medical Center Employee Occupational Health Services and the Centers for Disease Control and Prevention.2 Tuberculin skin tests (5 tuberculin U) were placed intradermally and read 48–72 hours later by experienced Employee Occupational Health Services nurses. Two-step testing was performed when indicated.2 All tuberculin skin test–positive (induration diameter greater than or equal to 10 mm) new employees had the QFT-G assay performed, typically on the day that the tuberculin skin test result was determined. Those with documented history of positive tuberculin skin test results (approximately 5% of the tuberculin skin test–positive individuals; the majority of these had a reading measured in millimeters from an outside institution) had QFT-G tests performed without tuberculin skin testing. Employees who had previously received at least 1 month of therapy for active TB or LTBI were excluded from QFT-G testing. All tuberculin skin test–positive new employees had a chest radiograph performed. QFT-G–positive employees were referred to the infectious diseases clinic for discussion of LTBI therapy.

Enrollment of participants

In the infectious diseases clinic, after deciding whether to receive 9 months of isoniazid therapy, all QFT-G–positive employees were invited to enroll in our study; enrollment commenced in November 2006. All participants signed written informed consent as approved by the Beth Israel Deaconess Medical Center Institutional Review Board.

Study protocol

Clinical data (including tuberculin skin test results, initial QFT-G test results, chest radiograph findings, and social history) for each participant were reviewed. Participants who had chosen to receive isoniazid treatment had repeat QFT-G tests performed before the initiation of isoniazid therapy (defined as baseline) and had serial QFT-G tests performed at 3, 6, 9, and 12 months after initiation of isoniazid therapy. At each time point, an extra tube of blood was collected for extended proliferation assays. In accordance with routine Employee Occupational Health Services protocol, all participants who were receiving therapy (300 mg of isoniazid and 50 mg of pyridoxine daily) presented monthly for discussion of medication compliance and symptoms, liver function testing, and new prescriptions. Participants who did not receive treatment had repeat QFT-G tests performed at the time of enrollment (baseline) and 12 months later.

QFT-G assay

Assays were performed according to the manufacturers’ recommendations.3 Accordingly, an interferon-γ concentration of 0.35 IU/mL in response to either early secretory antigenic target–6 or culture filtrate protein–10, relative to the negative control, was considered to be positive.

Extended proliferation assays

Extended proliferation assays were performed as described elsewhere,4 with use of purified protein derivative as the stimulatory antigen. In brief, human peripheral blood mononuclear cells (1 × 105 cells/well) were incubated in medium alone or in medium containing purified protein derivative (2 μg/mL). Plates were cultured for 6 days, pulsed with [methyl-3H]Thymidine for an additional 8 hours, harvested, and counted (in counts per minute).

Statistical analysis

Baseline and longitudinal data were analyzed using JMP software (version 7.0) and Microsoft Excel.


Participant enrollment and QFT-G testing

Over a 1-year period, we enrolled 28 QFT-G–positive individuals, 17 of whom chose to initiate isoniazid therapy and 11 of whom opted not to receive isoniazid. Of the 17 participants who chose to receive isoniazid therapy, 5 subsequently completed 9 full months of isoniazid therapy in addition to the entire serial testing protocol. Of the 11 participants who chose not to receive treatment, 4 had a 12-month follow-up QFT-G test performed. Therefore, we were able to fully evaluate 9 QFT-G–positive individuals who successfully completed the serial testing protocol, 5 of whom had chosen to receive isoniazid treatment and 4 of whom had chosen not to receive the treatment. Baseline clinical characteristics of the 9 participants are detailed in the Table. The 5 participants who received treatment had clearly documented tuberculin skin test readings of 15-24 mm (Table). All 5 of these participants had positive QFT-G test results at all 4 time points from baseline through the completion of the 9-month isoniazid treatment course (at 0, 3, 6, and 9 months) (Figure, panel A). Furthermore, 3 of the participants who received treatment had an additional QFT-G test performed 3 months after completing the isoniazid treatment course (at 12 months), and all of these patients remained QFT-G positive (Figure, panel A). When the absolute values of early secretory antigenic target–6 and culture filtrate protein–10 responses were examined longitudinally for each individual antigen in the 5 participants, there were no clear patterns of increase or decrease over time (data not shown).

Comparison of results of serial QuantiFERON-TB Gold (QFT-G) assays and proliferation assays for participants who received isoniazid treatment and those who did not receive isoniazid treatment. A, QFT-G results for the 5 treated participants (participants ...
Baseline Clinical Characteristics of QuantiFERON-TB Gold–Positive Participants Who Completed the Study

All participants who did not receive treatment had tests performed at enrollment and 12 months later. All 4 participants were still positive at 12 months (Figure, panel B). None of the treated or untreated participants reported any exposure to patients with active TB at any time during the serial testing protocol.

Proliferation assays

Extended proliferation assays in which purified protein derivative was used as stimulatory antigen were performed serially for all 9 participants in parallel with the QFT-G assays (Figure, panels C and D). For unknown reasons, participant 1 (who did not receive treatment) had extremely high background counts in the medium control well at baseline. Nonetheless, samples from all 9 participants strongly responded to purified protein derivative stimulation at all time points. These results support the original diagnosis of LTBI and suggest that isoniazid treatment does not affect (at least for 9–12 months) the overall immunologic reactivity to M. tuberculosis antigens.


Our goal in the present study was to examine the effect of standard LTBI treatment on QFT-G assay results in a US occupational health setting in which isoniazid treatment compliance was monitored and reexposure to active TB was not a confounding factor. Our study design reflects the fact that the QFT-G assay has been sanctioned by the Centers for Disease Control and Prevention for use in HCW screening programs.1 All 5 newly hired HCWs who initially tested positive by the QFT-G assay on routine screening and who then received 9 months of closely monitored isoniazid therapy continued to have positive QFT-G results at the time of treatment completion; 3 of these HCWs had repeat QFT-G tests performed 3 months later, and results for all 3 HCWs were still positive.

Previous studies of the effect of LTBI therapy on IGRA results611 have generated conflicting data, with some studies suggesting that responses to TB-specific antigens might decrease sufficiently with treatment that IGRAs might provide a useful means of monitoring treatment efficacy, and other studies suggesting that responses might not decrease sufficiently for IGRAs to be used for this purpose. However, the comparability of these studies to each other and to our study is confounded by multiple factors, including repeated exposure of individuals to active TB during and after LTBI treatment,9 differences in LTBI treatment protocols, compared with the current US standard,68,10,11 and differences in study populations (eg, studies of recent contacts).7,8,10,11 Our study uniquely assesses the impact of the US standard LTBI treatment course on QFT-G test results in newly hired HCWs who have received a diagnosis of LTBI by routine hospital screening protocols; the majority of these HCWs were likely to have been remotely infected.

Although our sample size is small, our results unequivocally indicate that QFT-G test results should not be used to assess the effectiveness of recent or remote treatment courses for LTBI: the majority of individuals who initially test positive by the QFT-G assay will continue to test positive after 9 months of isoniazid treatment. This result has significant implications for HCW screening programs, because it should not be assumed that HCWs who report prior LTBI therapy but who still test positive by the QFT-G assay have not received appropriate treatment in the past. Moreover, neither physicians nor patients should expect reliable changes in QFT-G test results after standard treatment. Finally, our results neither support nor refute the idea that a positive IGRA result, in the absence of treatment for LTBI, is associated with a higher risk of reactivation of LTBI, compared with a positive tuberculin skin test result alone. Only long-term follow-up of large numbers of persons will be able to address this topic.


We thank the Beth Israel Deaconess Medical Center employee health team, for their efforts in organizing QuantiFERON-Gold testing, and the Beth Israel Deaconess Medical Center phlebotomy team, for their help with obtainment of blood samples.

Financial support. Pittsfield Anti-Tuberculosis Association (grant to N.R.P.) and Clinical Investigator Training Program through Beth Israel Deaconess Medical Center–Harvard/Massachusetts Institute of Technology Health Sciences and Technology, in collaboration with Pfizer and Merck (to N.R.P.). QuantiFERON-TB Gold kits were provided by Cellestis.


Potential conflicts of interest. E.N. reports that he has served as a consultant to Oxford Immunotech. All other authors report no conflicts of interest relevant to this article.


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