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Acute myeloid leukemia (AML) with inversion of chromosome 3 is characterized by overexpression of EVI1 and carries a dismal prognosis. Arsenic-containing compounds have been described to be efficacious in malignancies overexpressing EVI1. Here, we describe a case of AML with inv(3)(q21q26.2) treated with the organic arsenical darinaparsin. Using a “personalized medicine approach,” two different arsenicals were screened for anti-leukemic effect against the patient’s cells ex vivo. The most promising compound, darinaparsin, was selected for in vivo treatment. Clinical effect was almost immediate, with a normalization of temperature, a stabilization of white blood cell (WBC) counts and an increased quality of life. Longitudinal monitoring of patient response and resistance incorporating significant correlative studies on patient-derived blood samples over the two cycles of darinaparsin given to this patient allowed us to evaluate potential mechanisms of response and resistance. The anti-leukemic effects of darinaparsin correlated with inhibition of the alternative NF-κB pathway and production of the inflammatory cytokine IL-8. Emergence of resistance was suspected during treatment cycle 2 and supported by xenograft studies in nude mice. Darinaparsin resistance correlated with an attenuation of the effect of treatment on the alternative NF-κB pathway. The results from this patient indicate that darinaparsin may be a good treatment option for inv(3) AML and that inhibition of the alternative NF-κB pathway may be predictive of response. Longitudinal monitoring of disease response as well as several correlative parameters allowed for the generation of novel correlations and predictors of response to experimental therapy in a heavily pretreated patient.
Acute myeloid leukemia (AML) with inversion or translocation of the long arm of chromosome 3 (inv(3; q21q26.2)/t(3;3; q21;q26.2), hereafter referred to as “inv(3)/t(3;3) AML” accounts for 1–2% of AML cases and predicts an extremely poor prognosis, with a 5-year overall survival of 5.7% (Lugthart et al., 2010). Due to the distinct clinicopathologic attributes associated with these chromosomal aberrations, inv(3)/t(3;3) AML has been included as a separate sub-category of “AML with recurrent genetic abnormalities” in the latest WHO classification (Swerdlow et al., 2008). Despite a characteristic clinical picture with a well-defined underlying molecular pathology, no targeted therapy currently exists for inv(3)/t(3;3) AML, although improved treatment regimens clearly are needed, as reflected by the poor overall performance of this patient category.
Molecularly, inv(3)/t(3;3) AML is characterized by the aberrant juxtaposition of the oncogene ecotropic viral integration site 1 (EVI1), with regulatory elements of ribophorin 1 (RPN1), resulting in overexpression of EVI1 (Buonamici et al., 2003). EVI1 is a transcription factor with a well-recognized role in the normal development of the hematopoietic system. It has been reported to function primarily as a transcriptional repressor, however, examples of an activating role in gene expression have also been described (Yuasa et al., 2005; Lugthart et al., 2011). Arsenic trioxide (ATO), an inorganic arsenical, has been investigated as a potential therapy for hematological diseases overexpressing EVI1. Shackelford et al. (2006) found that super-pharmacologic concentrations of ATO degrade EVI1, as well as several fusion proteins containing EVI1, in cell line models of AML. A clinical trial investigating the effect of ATO in combination with thalidomide in myelodysplastic syndrome (MDS), reported hematologic responses in three out of five patients with overexpression of EVI1, including responses in two out of three patients with inv(3)/t(3;3) (Raza et al., 2004). Here, we describe the treatment of a patient with inv(3) AML with the organic arsenical darinaparsin (reviewed in Mann et al., 2009). Although EVI1 expression was not modulated following treatment, we did monitor the response and development of resistance to darinaparsin. By assessing tumor gene expression longitudinally within the patient, we found changes in the alternative NF-κB pathway to be important correlates with response.
The patient was diagnosed with AML in 2005 at the age of 30. The karyotype performed on bone marrow cells at diagnosis was 46,XX,inv(3)(q21q26.2)×2(Figure 1A). She initially underwent two induction regimens followed by an allogeneic stem cell transplant, resulting in a complete remission. After 5years, the patient’s AML relapsed and she received a total of seven different chemotherapy-containing regimens in an effort to try to control her disease (see Table Table11 for details). Fluorescent in situ hybridization experiments confirmed the presence of a biallelic inversion of chromosome 3 (Figure (Figure1B).1B). Given previous reports suggesting that malignant cells with inv(3) might be sensitive to ATO, cells from the patient’s bone marrow were collected to determine response to arsenicals ex vivo (Raza et al., 2004; Shackelford et al., 2006). As shown in Figure Figure1C,1C, treatment with therapeutically attainable concentrations of darinaparsin induced significant cell-death, while ATO treatment only resulted in a negligible increase in cell death (Tsimberidou et al., 2009). Of note, ATO (As2O3) contains twice as much arsenic per mole as darinaparsin (C12H22AsN3O6S), thus, 1μM ATO contains equivalent amounts of arsenic as 2μM darinaparsin. Given these results, we requested approval from Health Canada to treat the patient with darinaparsin. However, her leukemia progressed rapidly, requiring additional high-dose salvage chemotherapy to control her disease before approval to administer darinaparsin could be obtained. Following the last high-dose regimen, the patient was hospitalized with cytopenia, persistent fever, and poor general condition corresponding to an ECOG performance status of 2. Ex vivo treatment of the patient’s peripheral blood mononuclear cells (PBMCs) immediately prior to starting darinaparsin treatment confirmed that tumor cell sensitivity to darinaparsin was intact (Figure (Figure11D).
Peripheral white blood cell (WBC) counts were measured each time the patient was seen at the hospital. As shown in Figure Figure2A,2A, darinaparsin immediately reduced the patient’s WBC counts and this effect was maintained while the patient remained on drug. The wave-like pattern in WBC counts, with two troughs per treatment cycle, suggests that darinaparsin exerted both immediate anti-cancer effects and more delayed effects. Microscopy of peripheral blood smears, showing nuclear blebbing, suggests cells were dying by apoptosis (Figure (Figure2B).2B). Induction of apoptosis has previously been described as a mechanism of anti-cancer activity of darinaparsin (Matulis et al., 2009).
Within 10h of the first darinaparsin infusion, the patient’s fever (Figure (Figure2C)2C) and night sweats resolved and she regained her appetite and energy. She was discharged home 3days after receiving the last dose of the first cycle. She enjoyed a good quality of life, with an ECOG performance status of 1 for more than 30days, while undergoing her second cycle of darinaparsin as an outpatient. Treatment was well tolerated with no observed side effects. The patient died from extramedullary manifestations and progression of her AML 36days after receiving the first dose of darinaparsin.
Given the rapid resolution of the patient’s symptoms following darinaparsin administration, we profiled levels of pro-inflammatory cytokines in plasma in response to treatment using a flow cytometry-based multiplex immunoassay kit. While all but three of the 11 cytokines investigated were below the limit of detection of the assay, high levels of interleukin (IL)-8 were detected prior to starting treatment (Figure (Figure2C).2C). IL-8 has both pro-survival and pro-proliferative properties and has previously been shown to be increased in AML patients (Waugh and Wilson, 2008; Kornblau et al., 2010). The patient’s pre-treatment IL-8 levels were about 10 times higher than what has been reported for normal controls (Kornblau et al., 2010). Interestingly, the changes observed in IL-8 plasma levels in response to darinaparsin treatment mirror the changes seen in the patient’s WBC counts (Figure (Figure2A)2A) and temperature (Figure (Figure2C),2C), suggesting that darinaparsin impacts temperature, as well as levels of leukemic cells and inflammatory cytokines (these effects may well be related) and that this may underlie the marked subjective and objective improvement in the patient’s symptoms. Pearson correlation coefficients between IL-8 expression, temperature, and WBC counts suggest that these three variables are indeed strongly positively related (Table (Table22).
To determine whether the delayed effects were due to arsenic accumulation in the patient, we measured elemental arsenic levels in patient plasma and PBMCs. Arsenic levels in plasma increased within the first 24h of treatment and continually rose throughout the treatment period (Figure (Figure2D).2D). After 4–5days off drug, plasma arsenic levels returned to baseline. Arsenic levels in PBMCs rose slower than plasma levels and remained elevated for several days after discontinuation of darinaparsin (Figure (Figure2E).2E). Thus, while it does not appear that arsenic accumulates in patient plasma under this dosing regimen, it seems that high arsenic levels in PBMCs are maintained several days after discontinuation of darinaparsin.
By continuously sampling during the treatment of this patient, we were able to perform correlative studies to investigate potential markers of response and resistance. A defining molecular pathology of inv(3)/t(3;3) AML is the aberrant overexpression of EVI1. Previous studies suggested that arsenic could decrease EVI1 expression, potentially contributing to the cytotoxic effects (Raza et al., 2004; Shackelford et al., 2006). To ascertain the effect of darinaparsin on EVI1, we first analyzed EVI1 protein expression levels in PBMCs by immunoblot. No change in EVI1 protein was observed during the first 72h of darinaparsin treatment (Figure (Figure3A).3A). After 72h, there was significant hemolysis of blood samples, preventing reliable quantification at later time-points. We also assessed whether the effect of darinaparsin observed in this patient was due to inhibition of EVI1’s gene regulatory activity by analyzing the expression levels of the tumor suppressor PTEN, a target gene repressed by EVI1, by quantitative PCR and western blot (Yoshimi et al., 2011). No induction of PTEN mRNA or protein was observed during the first 72h of treatment (Figures (Figures33A,B).
In order to determine the reproducibility of our results obtained in a single patient, we treated the EVI1-expressing myeloid leukemia cell line K562 with ATO and darinaparsin for 24 and 48hours. Analogous to what was observed in primary leukemic cells (Figures (Figures1C,D),1C,D), K562 cells were more sensitive to darinaparsin than to ATO treatment (Figure (Figure3C)3C) while neither darinaparsin nor ATO treatment modulated expression of EVI1 protein levels in K562 cells (Figure (Figure3D).3D). Thus, our results do not support modulation of EVI1 expression or repressive activity as part of the anti-leukemic mechanism of darinaparsin in either EVI1-expressing primary cells or cell lines. Note that decreased EVI1 mRNA expression was only described after several months in MDS patients treated with ATO and thalidomide (Raza et al., 2004).
Comparison of the in vivo WBC response to darinaparsin during cycle 1 with the response during cycle 2 suggests the emergence of resistance to darinaparsin (Figure (Figure2A).2A). In particular, the increase in WBC counts after darinaparsin infusion was stopped is greater in cycle 2 than in cycle 1. While the patient was still at home, enjoying a good quality of life at this time, these results suggest that resistance to darinaparsin was starting to develop. Further evidence of a resistant phenotype was provided by xenograft studies in nude mice (Figure (Figure4).4). In order to measure levels of leukemia initiating cells, we measured the engraftment of patient-derived PBMCs from a pre-treatment blood sample compared to PBMCs isolated during cycle 2. Engraftment is predictive of the number of leukemia initiating cells. Patient PBMCs isolated after 48h of darinaparsin treatment during cycle 2 showed greater levels of mouse marrow engraftment than pre-treatment PBMCs, suggesting elevated levels of leukemia initiating cells in the post-treatment sample from cycle 2.
To explore potential mechanisms of resistance to darinaparsin, we performed cDNA microarray analyses on PBMCs prior to and 48h after the start of each cycle of darinaparsin. The alternative NF-κB pathway was identified as one of the most modulated pathways during the course of darinaparsin treatment using Ingenuity Systems Pathway Analysis (Figure (Figure5A,5A, left panel). Expression patterns of three genes in the alternative NF-κB pathway were validated by quantitative PCR using gene-specific primers as shown in Figure Figure5B.5B. There is good agreement between results obtained by microarray and quantitative PCR. Darinaparsin decreased expression of several members of the alternative NF-κB pathway after 48h of treatment. This correlates with the observed decrease in production of IL-8 (Figure (Figure2C),2C), a well-characterized NF-κB target gene. Furthermore, gene expression in PBMCs prior to the second cycle of darinaparsin revealed an upregulated alternative NF-κB pathway that was not inhibited by darinaparsin during the second cycle, perhaps foreshadowing the emergence of resistance (Figure (Figure5A,5A, center and right panels). The attenuation of the response of the alternative NF-κB pathway to darinaparsin treatment coincides with emergence of resistance, thereby strengthening the hypothesis that this pathway plays a role in the sensitivity/resistance of inv(3) AML cells to darinaparsin.
Herein, we describe for the first time the treatment of a patient with inv(3) AML with the organic arsenical darinaparsin. Darinaparsin stabilized disease in a heavily pretreated patient and greatly improved quality of life. Therapy was well tolerated with almost immediate clinical benefit and no observed side effects. Clinical benefit was suggested by a simple ex vivo cell-death assay performed prior to starting therapy, indicating that this may be useful as a predictive marker of in vivo response. Unexpectedly, we did not find evidence that treatment directly affected EVI1 levels or activity. Instead, our results indicate that inhibition of the alternative NF-κB pathway by darinaparsin correlated with response and plasma levels of the inflammatory cytokine IL-8.
The strategy outlined here serves as an example of the power of a personalized medicine approach in patients for whom no accepted standard therapy is available. In particular, for patients suffering from uncommon types of malignancies, it is imperative to gather as much information on treatment effect, response, and resistance at every given opportunity. This series of experiments highlights how multiple platforms can be used to assess mechanisms of response and resistance. Here, we included everything from genomics to in vivo stem cell analysis.
In this specific case, the literature suggested that arsenicals might be efficacious in malignancies with inv(3), thus we tested two arsenicals using a simple ex vivo death assay. Interestingly, the drug that performed the best in our ex vivo assay was not ATO [the drug for which activity in inv(3) malignancies had previously been reported], but rather the organic arsenical darinaparsin (Raza et al., 2004; Shackelford et al., 2006; Mann et al., 2009). Darinaparsin has previously been tested in hematological malignancies, including acute leukemias (Clinical trial ID: NCT00592046). Encouragingly, the patient experienced marked subjective and objective improvement after starting darinaparsin, however, after about a month, her condition rapidly deteriorated. Our results highlight a key advantage of longitudinal sampling of the same patient over his or her entire disease trajectory. The availability of blood samples from both the time during which the malignant cells exhibited sensitivity to treatment (cycle 1) and the time at which resistance was starting to become apparent (cycle 2) allowed us to compare treatment responses at these time points. Using an unbiased gene expression based approach, we identified the alternative NF-κB pathway as being constitutively activated in the patient’s AML blasts prior to treatment and showed that this activation was attenuated after 48h of darinaparsin. However, prior to the start of cycle 2, the NF-κB pathway was upregulated further and was no longer responsive to darinaparsin treatment (Figure (Figure5).5). The abrogation of a putative mechanism of response at the time of emergence of resistance strengthens the hypothesis that this pathway plays an important role in resistance/sensitivity to darinaparsin in this patient. Levels of circulating IL-8 in plasma may function as a surrogate marker of NF-κB pathway activation and could conceivably be used to monitor treatment response in IL-8 secreting AML.
Based on the results outlined above, further investigation is warranted into (1) EVI1 overexpressing AMLs and (2) AMLs with activation of the alternative NF-κB pathway as potential targets for darinaparsin treatment.
All clinical investigations and treatments were performed with patient consent under a compassionate use protocol approved by the Jewish General Hospital Research Ethics Committee and Health Canada.
K562 cells were purchased from ATCC and grown in IMDM medium supplemented with 10% fetal bovine serum and antibiotics.
G-banded karyotypes were obtained from bone marrow mononuclear cells according to standard cytogenetic procedures and described according to the International System for Human Cytogenetic Nomenclature 2009. FISH experiments were performed on interphasic nuclei and metaphases using the EVI1 Breakapart probe LPH036 (Cytocell, Cambridge, UK).
Peripheral blood was collected in heparin tubes prior to starting treatment and every time the patient was seen in hospital. The blood was spun down to remove the plasma (stored at −80°C) followed by isolation of PBMCs by Ficoll gradient separation. RNA, DNA, and whole cell extracts were isolated as described below. Surplus PBMCs were frozen in 10% DMSO/90% fetal bovine serum at −80°C.
For a detailed description see Hardin et al. (1992). Briefly, cells were washed once in FACS wash buffer (5mM NaN3 in PBS supplemented with 5% fetal bovine serum) and then stained with 50μg/mL PI (Sigma) in hypotonic buffer (0.1% sodium citrate and 0.1% Triton X-100 in ddH20). Approximately 500,000 cells/condition were stained with 0.5mL PI stain and analyzed on a BD Biosciences FACSCalibur flow cytometer. Cell death was measured as the percentage of cells with sub-G0 DNA content.
Elemental arsenic levels were measured in patient plasma and PBMCs by inductively coupled plasma mass spectrometry (Chemical Solutions Ltd, Mechanicsburg, PA, USA).
Whole cell extracts were made from patient samples and K562 cells using Tris/NaCl/triton x-100 buffer (50mM Tris-HCl pH 8.0, 150mM NaCl and 1% triton X-100 supplemented with protease inhibitor cocktail (Roche) and PhosStop (Roche)(. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membrane. Primary antibodies for PTEN, EVI1, and GAPDH were all purchased from Cell Signaling. Specific binding was detected with horseradish peroxidase-labeled secondary antibodies and visualized with enhanced chemiluminescence.
RNA was extracted from patient samples using Qiagen AllPrep DNA/RNA mini kit. RNA was converted to cDNA using Superscript II reverse transcriptase (Invitrogen). Specific qPCR amplification was performed using a 7500 Fast Thermocycler (Applied Biosystems) and either Taqman chemistry (PTEN, 18S, and GAPDH) or SYBR green chemistry and specific primers. BIRC3: 5′-TCC GTC AAG TTC AAG CCA GTT-3′ and 5′-GGG CTG TCT GAT GTG GAT AGC-3′; TNFSF1B: 5′-GGC CAG ACC AGG AAC TGA AA-3′ and 5′-GAT GAA GTC GTG TTG GAG AAC GT-3′; and NFkB2: 5′-ACG AGG GAC CAG CCA AGA T-3′ and 5′-GCA CGA GGT GGG TCA CTG T-3′.
Cytokine profiling of patient plasma samples was done using a Human Th1/Th2 11plex FlowCytomix Multiplex kit (eBioscience, San Diego, CA, USA) according to the manufacturer’s instructions.
Studies were performed with approval from the University of Rochester Institutional Review Board, IUCOC. NOD/SCID/IL2Rγc mice were sublethally irradiated with 2.7Gy (270rad) using a RadSource X-ray irradiator (RadSource, Boca Raton, FL, USA) the day before transplantation. Cells to be assayed were thawed, counted, and then injected via tail vein in a final volume of 0.2mL of PBS with 0.5% FBS. After 12weeks, animals were sacrificed and BM was collected. To determine human cell engraftment the BM cells were labeled with antihuman CD45 (Becton Dickinson) and analyzed via flow cytometry.
Microarray analyses were performed using Agilent (Mississauga, ON, USA) Human 60K expression arrays at Genome Quebec. Data were analyzed using FlexArray and Ingenuity Pathway Analysis Software.
Raw text files for the one-color Agilent array were imported in R version 2.14.0 using the package limma (PMID 16646809). We processed the data by first subtracting the background using normexp, took the log2 of the remaining intensity and applied quantile normalization to the entire set of arrays. Differential expression of genes was computed using limma (PMID 16646809). We considered genes as differentially expressed if the mean normalized signal of the two conditions is higher than five and the differential expression is greater than twofold change.
Darinaparsin (300mg/m2) was administered by intravenous infusion on five consecutive days followed by 16days off drug. Following the end of the first 21-day cycle, this protocol was repeated for cycle 2.
For in vitro experiments, all error-bars represent the standard error of the mean of three replicates. Significance was determined by one-way analysis of variance followed by Newman–Keuls post-tests usingPrism version 3.0 (GraphPad software, San Diego, CA, USA). For the mouse work, significance was calculated using an unpaired t-test (two-tailed).
*, p<0.05; **, p<0.01; ***, p<0.001.
Pearson correlations in Table Table22 were calculated using the correlation function in Microsoft Excel with the data points shown.
Robert A. Morgan is Senior Vice President, Regulatory Affairs and Quality/Pharmaceutical Development of ZIOPHARM Oncology, Inc. and a stockholder of ZIOPHARM. ZIOPHARM is the licensee of darinaparsin and is developing darinaparsin for various indications in oncology.
The authors wish to thank Dr. Hugues de Thé for helpful suggestions. We also would like to acknowledge Christian Young for expert assistance with flow cytometric analyses and Genome Québec for assistance with gene expression microarray analysis. Finally, we wish to thank our patient for participating in clinical research. Funding: This work was supported by a grant from the Canadian Institutes of Health Research and the Canadian Foundation for Innovation. Torsten H. Nielsen holds a Cole Foundation fellowship. Kevin P. Callahan holds a fellowship from the American Cancer Society. Koren K. Mann is a chercheur-boursier of the Fonds de la recherche en santé-Québec (FRSQ).