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BMC Infect Dis. 2013; 13: 35.
Published online Jan 24, 2013. doi:  10.1186/1471-2334-13-35
PMCID: PMC3565906
Antimicrobial susceptibility and genetic characteristics of Neisseria gonorrhoeae isolates from India, Pakistan and Bhutan in 2007–2011
Sunil Sethi,1 Daniel Golparian,2 Manju Bala,3 Dorji Dorji,4 Muhammad Ibrahim,2 Kausar Jabeen,5 and Magnus Unemocorresponding author2
1Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
2WHO Collaborating Centre for Gonorrhoea and other STIs, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, SE-701 85, Örebro, Sweden
3WHO GASP SEAR Regional Reference Laboratory, Apex Regional STD Teaching, Training & Research Centre, VMMC & Safdarjang Hospital, New Delhi, India
4JDW/NR Hospital, Thimphu, Bhutan
5The Aga Khan University, Karachi, Pakistan
corresponding authorCorresponding author.
Sunil Sethi: sunilsethi10/at/hotmail.com; Daniel Golparian: dangohh/at/gmail.com; Manju Bala: manjubala_2/at/hotmail.com; Dorji Dorji: karmadoj08/at/gmail.com; Muhammad Ibrahim: ibrahimbaseer/at/hotmail.com; Kausar Jabeen: kausar.jabeen/at/aku.edu; Magnus Unemo: magnus.unemo/at/orebroll.se
Received November 1, 2012; Accepted January 22, 2013.
Background
Knowledge on antimicrobial drug resistance and genetic characteristics of Neisseria gonorrhoeae isolates circulating in India, Pakistan, and Bhutan is sorely lacking. In this paper, we describe the prevalence of antimicrobial resistance and molecular characteristics of N. gonorrhoeae isolates from India, Pakistan, and Bhutan in 2007–2011.
Methods
Antimicrobial susceptibility and β-lactamase production were tested for 65 N. gonorrhoeae isolates from India (n=40), Pakistan (n=18) and Bhutan (n=7) using Etest methodology (eight antimicrobials) and nitrocefin solution, respectively. Resistance determinants, i.e. penA, mtrR, porB1b, gyrA, and parC, were sequenced. N. gonorrhoeae multiantigen sequence typing (NG-MAST) was performed for molecular epidemiology.
Results
The highest resistance level was observed for ciprofloxacin (94%), followed by penicillin G (68%), erythromycin (62%), tetracycline (55%), and azithromycin (7.7%). All the isolates were susceptible to ceftriaxone, cefixime, and spectinomycin. Thirty-four (52%) of the isolates were producing β-lactamase. No penA mosaic alleles or A501-altered alleles of penicillin-binding protein 2 were identified. Forty-nine NG-MAST STs were identified, of which 42 STs have not been previously described worldwide.
Conclusions
Based on this study, ceftriaxone, cefixime, and spectinomycin can be used as an empirical first-line therapy for gonorrhoea in India, Pakistan, and Bhutan, whereas ciprofloxacin, penicillin G, tetracycline, erythromycin, and azithromycin should not be. It is imperative to strengthen the laboratory infrastructure in this region, as well as to expand the phenotypic and genetic surveillance of antimicrobial resistance, emergence of new resistance, particularly, to extended-spectrum cephalosporins, and molecular epidemiology.
Keywords: Gonorrhoea, Antimicrobial resistance, penA, Ceftriaxone, Southeast Asia, India, Bhutan, Pakistan
Gonorrhoea, caused by Neisseria gonorrhoeae, is the most common bacterial sexually transmitted infection (STI), with 106.1 million cases among adults estimated in 2008 across the globe [1]. This incidence, according to the World Health Organization (WHO) global estimates, represented a 21% increase since 2005 [1,2]. Accordingly, gonorrhoea is associated with high morbidity and socioeconomic consequences, remaining a major worldwide public health problem [3-5]. During the past 60–70 years, N. gonorrhoeae has repeatedly demonstrated its enormous ability to develop resistance in a rapid manner to all antimicrobial drugs introduced for first-line therapy, along with a large capacity for the prompt spread of the emerged resistant strains globally [3,4,6-12]. In recent decade in vitro resistance and treatment failures with the currently recommended treatment regimens for gonorrhoea, the extended-spectrum cephalosporins (ESCs) cefixime and ceftriaxone, have been verified in Japan and Europe [13-21]. The threat of widespread resistance to ceftriaxone (the last remaining treatment option for single antimicrobial therapy) and untreatable gonorrhoea is real [6,16,19,22]. In response to this emergent situation the WHO has published its ‘Global Action Plan to Control the Spread and Impact of Antimicrobial Resistance in Neisseria gonorrhoeae’ [5,23], and subsequently, the European Centre for Disease Prevention and Control (ECDC) and the Center for Disease Control and Prevention (CDC) in the United States have launched their supplementary regional response plans [24,25].
Mutations in the penA gene encoding the penicillin-binding protein 2 (PBP2) is the main determinant for decreased susceptibility and resistance to ESCs. Acquisition of a penA mosaic gene or an alteration of amino acid A501 in PBP2 result in increased minimum inhibitory concentrations (MICs) of ESCs [12,26,27]. Furthermore, mutations in the promoter or coding sequence of the repressor gene mtrR cause over-expression of the MtrCDE efflux pump system that export the ESCs out from the cell, and alterations of amino acid G101 and A102 in the porin PorB1b (the penB resistance determinant), which is encoded by the porB1b gene, result in decreased influx of the ESCs. Those mechanisms further decrease the susceptibility to ESCs as well as to other antimicrobials such as penicillins, tetracyclines and macrolides [11,12,28,29]. Resistance to ciprofloxacin is caused by initial mutations in the gyrA gene (in particular in codon 91 and 95) that encode an altered subunit GyrA of the enzyme DNA Gyrase, with subsequent mutations in parC (encoding an altered subunit ParC of the enzyme DNA Topoisomerase IV) to reach high-level resistance [28,30].
A wide variety of antimicrobials, such as ESCs, but also penicillins, fluoroquinolones, macrolides, tetracycline and spectinomycin, may still be used for the treatment of gonorrhoea in India and Pakistan. Resistance to penicillin, ciprofloxacin, and tetracycline has been reported in India, Sri Lanka, Pakistan, and Bangladesh [31-37]. Further, exceedingly rare resistance (mainly single isolates) to spectinomycin and azithromycin has been observed in India and Bangladesh [32,36,37]. Finally, decreased susceptibility to ESCs has been noted in India and Bangladesh, whereas resistance to ESCs has not yet been reported from this region [31-37]. However, few isolates from this region have been examined and, in most cases, disc diffusion methods that do not reflect the exact MIC have been used for antimicrobial susceptibility testing rather than quality assured, internationally validated methods to determine the exact MIC. Furthermore, genetic antimicrobial resistance determinants, with the exception of determinants for ciprofloxacin resistance [38], have never been studied.
Moreover, it is important to have detailed knowledge about the gonococcal strain populations circulating in different communities, temporal and geographical changes of the populations, and the emergence and transmission patterns of individual strains for the prevention and control of infection. Thus, a highly discriminative, objective, and reproducible characterisation of N. gonorrhoeae strains can be valuable [39]. Genotypic methods based on DNA sequencing are internationally recommended, of which N. gonorrhoeae multiantigen sequence typing (NG-MAST) [40] or full- or extended-length porB gene sequencing are currently the best methods for fast, objective, portable, highly discriminatory, reproducible, typeable, and high-throughput characterisation [39]. In India and Pakistan gonococcal epidemiology has been explored in a few, mostly outdated studies, using traditional, low discriminatory phenotypic typing methods, such as antimicrobial susceptibility testing, auxotyping, plasmid profiling, and serotyping [41-45]. Only a few minor studies have used genetic gel-based typing methods (e.g., restriction fragment length polymorphism of the whole genomic DNA [46], ribotyping, and Opa-typing [47]). Unfortunately, all these methods suffer from a lack of an objective reading and interpretation of results (compared to sequencing-based methods), international standardisation, and a database for international interlaboratory comparisons [39].
The present study aimed to investigate antimicrobial susceptibility, genetic resistance determinants focussing on extended-spectrum cephalosporins and ciprofloxacin, and the molecular epidemiology of N. gonorrhoeae strains that were circulating in India, Pakistan, and Bhutan in 2007–2011. Internationally recommended, validated, and quality assured methods were applied for antimicrobial susceptibility testing and molecular epidemiology (NG-MAST).
N. gonorrhoeae patients and isolates
All viable clinical isolates of N. gonorrhoeae collected at the VMMC & Safdarjang Hospital, New Delhi, India (n=40; 2007 (3), 2008 (1), 2009 (17), 2010 (19)), Aga Khan University, Karachi, Pakistan (n=18; 2008 (1), 2009 (4), 2010 (3), 2011 (10)), and JDW/NR Hospital, Thimphu, Bhutan (n=7; all from 2010) during 2007–2010 were examined. The isolates were initially cultured from specimens from the urethra (males, n=60) and cervix (females, n=5) of patients presenting with symptoms and signs of gonorrhoea, and verified as N. gonorrhoeae using growth characteristics on selective culture agar media, Gram staining and oxidase test. All gonococcal isolates were cultured and stored (in −70°C freezer or lyophilized) as an integral part of the routine diagnostics and no personal identification data of any patient was handled. Accordingly, no ethical approval was required. The N. gonorrhoeae isolates were subsequently transported to the WHO Collaborating Centre for Gonorrhoea and other STIs, Örebro University Hospital, Örebro, Sweden, where the isolates were recultured and verified as N. gonorrhoeae using growth characteristics on the selective modified Thayer-Martin media, Gram staining, oxidase test and the MicroTrak N. gonorrhoeae Culture Confirmation Test (Trinity Biotech Plc, Co Wicklow, Ireland). All further testing was performed at the WHO Collaborating Centre for Gonorrhoea and other STIs in Sweden.
The 2008 WHO N. gonorrhoeae reference strains [30] were used as quality controls in all antimicrobial susceptibility testing and genetic characterisation (see below).
Antimicrobial susceptibility testing
MIC (mg/L) of all isolates were determined for ceftriaxone, cefixime, penicillin G, tetracycline, erythromycin, azithromycin, ciprofloxacin, and spectinomycin using Etest methodology on Difco GC Medium Base (Becton, Dickinson and Company, Sparks, MD, USA) supplemented with 1% BBL IsoVitaleX Enrichment (Becton, Dickinson and Company, Sparks, MD, USA), according to the instructions from the manufacturer (AB bioMérieux, Solna, Sweden). MIC breakpoints used for determination of susceptibility, intermediate susceptibility, and resistance (Table (Table1)1) were largely in accordance with the European Committee on Antimicrobial Susceptibility Testing (EUCAST (http://www.eucast.org); clinical breakpoints, v2.0). Production of β-lactamase was identified by nitrocefin discs (Oxoid, Basingstoke, Hants, England).
Table 1
Table 1
Antimicrobial susceptibility of 65 Neisseria gonorrhoeae isolates from India (n=40), Pakistan (n=18), and Bhutan (n=7) in 2007–2011
Isolation of genomic DNA
Genomic DNA was isolated according to the manufacturer’s instructions in a robotised MagNA Pure instrument (Roche, Basel, Switzerland) using the MagNA Pure LC DNA Isolation Kit III.
Determination of genetic antimicrobial resistance determinants
PCR amplification and sequencing of gonococcal genetic resistance determinants focussing on ESCs and ciprofloxacin, i.e. the penA, mtrR, penB, gyrA, and parC genes, were performed as described elsewhere [30].
Molecular epidemiological characterisation
The more variable segments of porB (490 bp) and tbpB gene (390 bp) examined in NG-MAST [40] were sequenced as described previously [48]. NG-MAST allele numbers and sequence types (STs) were assigned on the NG-MAST website (http://www.ng-mast.net).
Antimicrobial susceptibility testing
The results of the antimicrobial susceptibility of the N. gonorrhoeae isolates (n=65) are summarised in Table Table1.1. Briefly, the resistance was highest to ciprofloxacin (94%), followed by penicillin G (68%), erythromycin (62%), tetracycline (55%), and azithromycin (7.7%). All isolates (100%) were susceptible to ceftriaxone (MIC range: <0.002-0.064 mg/L), cefixime (MIC range: <0.016-0.064 mg/L), and spectinomycin (MIC range: 4–16 mg/L). β-lactamase was produced by 52% (n=34) of the isolates (India: n=22, 55%): Pakistan: n=6, 33%); Bhutan: n=6, 86%)); all those isolates were considered resistant to penicillin G (Table (Table11).
Genetic characterisation of penA alleles
No penA mosaic alleles or A501-altered alleles of PBP2 were identified (Table (Table2).2). According to the previously described numbering of PBP2 amino acid sequences in N. gonorrhoeae[16], PBP2 allele IX was the most prevalent (22 isolates), followed by II (n=19), XIX (n=12), IV (n=7), Modified-XIV (n=3), XII (n=1), and XXXV (n=1). Of these PBP2 alleles, all except PBP2 XXXV contain an insertion of aspartate in amino acid position 345 (D345a), which explains the high level of intermediate susceptibility and resistance to penicillin among the isolates [11,28] (Table (Table22).
Table 2
Table 2
Genetic resistance determinants for ceftriaxone, cefixime and ciprofloxacin in Neisseria gonorrhoeae isolates from India (n=40), Pakistan (n=18), and Bhutan (n=7) in 2007–2011
Genetic characterisation of resistance determinants that increase the efflux or decrease the influx of antimicrobials
Of the 65 examined isolates, 27 (42%) showed mutations in the inverted repeat region of the mtrR promoter (A-deletion (n=24) and T-insertion (n=3)), while G45D amino acid substitution in the DNA-binding motif of MtrR was found in seven (11%) isolates, of which three contained additionally an A-deletion in the mtrR promoter region. Furthermore, one isolate from Pakistan displayed the recently described C-to-T transition mutation 120 bp upstream of the mtrC start codon, termed mtr120, that results in a novel consensus −10 element and generation of a novel promoter for mtrCDE transcription. This mutation also results in an over-expression of the MtrCDE efflux pump [29] (Table (Table2).2). Of the 15 azithromycin resistant or intermediately susceptible isolates, 11 showed the A-deletion and two a T-insertion in the mtrR promoter. Furthermore, penB alterations, causing a decreased influx of antimicrobials [12,26,28], were found in 60 of the 65 isolates (92%).
In the absence of penA mosaic alleles or A501-altered PBP2 amino acid sequences, isolates containing both the mtrR and penB resistance determinants still did not have substantially enhanced MICs of the ESCs (ceftriaxone MIC range: 0.002-0.064 mg/L; cefixime MIC range: <0.016-0.064 mg/L), that is, as compared with the remaining isolates (ceftriaxone MIC range: <0.002-0.064 mg/L; cefixime MIC range: <0.016-0.064 mg/L).
Genetic characterisation of ciprofloxacin resistance determinants
In the present study, gyrA ciprofloxacin resistance mutations were found universally: for example, S91F mutations were identified in all 65 (100%) isolates. In addition, gyrA D95G mutations were found in 45% of the isolates, D95N in 43%, D95A in 9.2%, and D95Y in 1.5%. Concerning parC, A91G mutations were identified in 33.8% of the isolates, followed by A91K in 13.8%, A92Q in 4.6%, S87N in 4.6%, D86N in 3.1%, S82I in 1.5%, and S87R in 1.5% (Table (Table22).
Molecular epidemiological characterisation
Among the 65 isolates, 49 NG-MAST STs were identified (Additional file 1: Table S1), of which 42 were novel STs (India: n=27; Pakistan: n=11; Bhutan: n=4). The predominant STs were ST6058 (n=5: Indian isolates), ST6057 (n=4: Indian isolates), and ST6064 (n=3: Bhutan isolates). The remaining STs were represented by only one (39 STs) or two isolates (7 STs).
This study reports the antimicrobial susceptibility/resistance and molecular characteristics of N. gonorrhoeae isolates from India, Pakistan, and Bhutan in 2007–2011. A high prevalence of resistance was observed for ciprofloxacin (94%), penicillin G (68%), erythromycin (62%), and tetracycline (55%). These data are largely in accordance with previous surveys in the Southeast Asian region. Accordingly, in India, penicillin resistance has varied from 20% to 79%, tetracycline resistance from 0% to 97%, and ciprofloxacin resistance from 11% to 100% [34,49]. In Sri Lanka, 97% and 8.2% resistance have been reported to penicillin and ciprofloxacin, respectively, and in Bangladesh resistance to ciprofloxacin, penicillin, and tetracycline was found to be 76%, 33% and 57%, respectively [34]. Finally, in Pakistan 92%, 87%, and 78% resistance to ofloxacin, penicillin G, and tetracycline, respectively, has been noted [35].
Thus, in the present study none of the isolates was susceptible to ciprofloxacin, with 94% and 6% of the isolates being resistant and intermediately susceptible, respectively. gyrA mutations (S91F) were found universally, with many of the isolates containing additional mutations in the quinolone resistance determining regions of the gyrA and parC genes (Table (Table2),2), which confer a high level of resistance to fluoroquinolones (e.g., ciprofloxacin) [28,30,38]. The very high rate of fluoroquinolone resistance may be an indicator of the overuse and misuse of this class of antimicrobials in this region of the world, as caused by over-the-counter availability, unregulated and counterfeit medicines, self-medication or unqualified practitioners who prescribe a full range of treatments [49,50]. Although fluoroquinolones are no longer recommended for first-line treatment of gonorrhoea in most parts of the world [4,11,51-54], most worryingly they are still being used excessively by, in particular, private practitioners and quacks in Southeast Asia.
Another very commonly prescribed antimicrobial in the Southeast Asian region is azithromycin, which is frequently used in syndromic management of STIs because of the convenience of single oral dose therapy for many infections and its efficacy against several STI pathogens, including N. gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Haemophilus ducreyi, Klebsiella granulomatis, and Treponema pallidum[53,55]. Previously, only some single N. gonorrhoeae isolates with resistance to azithromycin have been reported in India and Bangladesh [36,37]. However, in the present study only 77% of the isolates were susceptible to azithromycin and 23% showed resistance (7.7%) or intermediate susceptibility (15%), showing its unsuitability for use as an empirical first-line therapy for gonorrhoea in this region. Furthermore, resistance to azithromycin, including very high-level resistance, has been described in many countries globally [4,11,12,28,56-60]. Of the 15 isolates that showed resistance or intermediate susceptibility to azithromycin in the present study, 13 (87%) showed mutations in the mtrR promoter, which enhances the expression of the MtrCDE efflux pump, exporting azithromycin out from the gonococcal cells and thus confers increased azithromycin MICs in these isolates [11,12,28,29].
Among the isolates examined in this study, no resistance was observed to ceftriaxone, cefixime, and spectinomycin. Accordingly, ceftriaxone, cefixime, and spectinomycin can be recommended as an empirical first-line therapy of gonorrhoea in this region, although judicious use of these antimicrobials (particularly spectinomycin) is imperative. Some single isolates with spectinomycin resistance and strains with “less susceptibility” to ceftriaxone have been reported in India and Bangladesh [32-34,37]. However, few isolates from this region have been examined and mostly disc diffusion methods (with lower breakpoints for “less susceptibility” to ceftriaxone and resistance to spectinomycin compared with international MIC-determining resistance methods) for antimicrobial susceptibility testing have been used rather than internationally validated, quality assured MIC-determining methods. Nevertheless, our data, including MICs of up to 0.064 mg/L for ceftriaxone and cefixime, emphasise the importance of promptly implementing expanded antimicrobial resistance surveillance in India, Pakistan, and Bhutan. Corresponding to the absence of resistance to ESCs, no penA mosaic alleles or A501-altered PBP2 amino acid sequences were found in any of the isolates. These alterations of the penA gene are critical for resistance to cefixime and ceftriaxone [11-13,16,19].
Disquietingly, a majority of the gonococcal infections (as well as other STIs) in India, Pakistan, and Bhutan remain undiagnosed (using laboratory testing), or go unreported, or both. STIs are also still considered a taboo, and accordingly, people do not visit physicians with their ailment. In other cases the patients have been inadequately treated multiple times when they reach the hospitals, or the samples are inadequately transported to the laboratories, both of which diminish the likelihood of recovering gonococcus in culture. Moreover, most of the laboratories are not well equipped to provide adequate culture and characterisation facilities. Hence, there is a need to strengthen the existing system by providing more resources and training in this region for enhanced surveillance and detection of gonorrhoea and other STIs.
The N. gonorrhoeae population in India, Pakistan, and Bhutan was found to be highly diversified, with 49 NG-MAST STs identified among the 65 isolates examined (Additional file 1: Table S1). The high number of unique STs (n=39) may be a consequence of random sampling (only viable isolates examined) over several years, sub-optimal diagnostic procedures, incomplete epidemiological surveillance and ineffective contact tracing, local emergence of new STs, and import of strains from abroad. Nevertheless, some minor ST clusters were identified, indicating multiple sexual transmission chains. Furthermore, of the 18 isolates from Pakistan, one isolate was assigned ST368 that also was found in India, suggesting circulation of a few common lineages between the two countries, whereas 11 isolates were of new STs. One isolate from India also belonged to ST1407, which has been shown to be a internationally spread successful gonococcal clone that accounts for the majority of the decreased susceptibility and resistance to ESCs and treatment failures with cefixime worldwide [12,17-19,61,62]. This clone has also shown its ability to develop high-level resistance to ceftriaxone [19]. Surprisingly, the ST1407 isolate from India did not contain the penA mosaic allele XXXIV (instead it contained the penA allele XXXV) [16] that has been strongly associated with ST1407 [12,17,20,61,63]. Accordingly, despite showing high-level resistance to ciprofloxacin and intermediate susceptibility to azithromycin, the Indian ST1407 isolate displayed low MICs of ceftriaxone (0.008 mg/L) and cefixime (<0.016 mg/L). The seven isolates from Bhutan (belonging to ST6061, ST6062, ST6063, and ST6064) did not share STs with any of the isolates from India or Pakistan.
Because of the small number of isolates examined in the present study, the selection bias for these isolates cultured during several years and the high number of gonococcal infections remaining undiagnosed using laboratory testing in the included countries, the results of the present study need to be interpreted with caution. In future studies, additional isolates will hopefully be available as well as epidemiological data linked to the gonococcal isolates.
N. gonorrhoeae strains circulating in India, Pakistan, and Bhutan are genetically highly diverse and exhibit a high resistance to ciprofloxacin, penicillins, erythromycin, and tetracycline. Furthermore, the resistance or intermediate susceptibility to azithromycin was also high. Consequently, cefixime, ceftriaxone, and spectinomycin, which all isolates were susceptible to, are the only antimicrobials that can be recommended for empirical first-line therapy of gonorrhoea in this region. It is essential to strengthen diagnostics capabilities, case reporting, and contact tracing, as well as the surveillance of gonorrhoea and the antimicrobial susceptibility patterns and new emergence of resistance to, in particular, the ESCs (i.e. cefixime and ceftriaxone) in Southeast Asia. Finally, it is also imperative to inform timely and evidence-based update treatment recommendations.
Competing interests
The authors declare that they no competing interest.
Authors’ contributions
MU, MB, DG, and SS designed the study, analysed, and interpreted the data. MB, DD, and KJ performed all the initial diagnostics and provided the gonococcal isolates. DG, MI, and SS performed all the laboratory analyses. All authors were involved in the preparation of this paper. All authors read and approved the final manuscript.
Pre-publication history
The pre-publication history for this paper can be accessed here:
Supplementary Material
Additional file 1: Table S1
Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) STs of Neisseria gonorrhoeae isolates from India (n=40), Pakistan (n=18), and Bhutan (n=7) in 2007–2011.
Acknowledgments
This work was supported by the Örebro County Council Research Committee and the Foundation for Medical Research at Örebro University Hospital, Sweden, and the Indian Council of Medical Research (ICMR) that provided an International Fellowship to Sunil Sethi.
  • World Health Organization (WHO) Global incidence and prevalence of selected curable sexually transmitted infections - 2008. Geneva: World Health Organization; 2012. Available at: http://www.who.int/reproductivehealth/publications/rtis/2008_STI_estimates.pdf (Accessed: December 20, 2012)
  • World Health Organization (WHO) Prevalence and incidence of selected sexually transmitted infections: Chlamydia trachomatis, Neisseria gonorrhoeae, syphilis and Trichomonas vaginalis. Methods used by WHO to generate 2005 estimates. Geneva: World Health Organization; 2011. ISBN:978 92 4 150245 0.
  • Tapsall J. Antimicrobial resistance in Neisseria gonorrhoeae. WHO/CDS/DRS/2001.3:16. Geneva: World Health Organization; 2001.
  • Tapsall JW, Ndowa F, Lewis DA. et al. Meeting the public health challenge of multidrug- and extensively drug-resistant Neisseria gonorrhoeae. Exp Rev Anti Infect Ther. 2009;7:821–834. doi: 10.1586/eri.09.63. [PubMed] [Cross Ref]
  • World Health Organization (WHO) Department of Reproductive Health and Research. Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae. Geneva: WHO; 2012. pp. 1–36. Available at: http://www.who.int/reproductivehealth/publications/rtis/9789241503501 (Accessed: December 20, 2012)
  • Bolan GA, Sparling PF, Wasserheit JN. The emerging threat of untreatable gonococcal infection. N Engl J Med. 2012;366:485–487. doi: 10.1056/NEJMp1112456. [PubMed] [Cross Ref]
  • Cole MJ, Unemo M, Hoffmann S. et al. The European gonococcal antimicrobial surveillance programme, 2009. Euro Surveill. 2011;16:pii=19995.
  • WHO Western Pacific and South East Asian Gonococcal Antimicrobial Surveillance Programmes. Surveillance of antibiotic resistance in Neisseria gonorrhoeae in the WHO Western Pacific and South East Asian Regions, 2010. Commun Dis Intell. 2012;36:95–100. [PubMed]
  • Kirkcaldy RD, Ballard RC, Dowell D. Gonococcal resistance: are cephalosporins next? Curr Infect Dis Rep. 2011;13:196–204. doi: 10.1007/s11908-011-0169-9. [PubMed] [Cross Ref]
  • Kubanova A, Frigo N, Kubanov A. et al. The Russian gonococcal antimicrobial susceptibility programme (RU-GASP)–national resistance prevalence in 2007 and 2008, and trends during 2005–2008. Euro Surveill. 2010;15:pii=19533. [PubMed]
  • Unemo M, Shafer WM. Antibiotic resistance in Neisseria gonorrhoeae: origin, evolution, and lessons learned for the future. Ann N Y Acad Sci. 2011;1230:E19–E28. doi: 10.1111/j.1749-6632.2011.06215.x. [PubMed] [Cross Ref]
  • Unemo M, Nicholas R. Emergence of multidrug-resistant, extensively drug-resistant and untreatable gonorrhea. Future Microbiol. 2012;7:1401–1422. doi: 10.2217/fmb.12.117. [PubMed] [Cross Ref]
  • Cámara J, Serra J, Ayats J. et al. Molecular characterization of two high-level ceftriaxone-resistant Neisseria gonorrhoeae isolates detected in Catalonia, Spain. J Antimicrob Chemother. 2012;67:1858–1860. doi: 10.1093/jac/dks162. [PubMed] [Cross Ref]
  • Deguchi T, Yasuda M, Yokoi S. et al. Treatment of uncomplicated gonococcal urethritis by double-dosing of 200 mg cefixime at a 6-h interval. J Infect Chemother. 2003;9:35–39. doi: 10.1007/s10156-002-0204-8. [PubMed] [Cross Ref]
  • Ison CA, Hussey J, Sankar KN. et al. Gonorrhoea treatment failures to cefixime and azithromycin in England, 2010. Euro Surveill. 2011;16(14):pii=19833. [PubMed]
  • Ohnishi M, Golparian D, Shimuta K. et al. Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: detailed characterization of the first strain with high-level resistance to ceftriaxone. Antimicrob Agents Chemother. 2011;55:3538–3545. doi: 10.1128/AAC.00325-11. [PMC free article] [PubMed] [Cross Ref]
  • Unemo M, Golparian D, Syversen G. et al. Two cases of verified clinical failures using internationally recommended first-line cefixime for gonorrhoea treatment, Norway, 2010. Euro Surveill. 2010;15(47):pii=19721. [PubMed]
  • Unemo M, Golparian D, Stary A. et al. First Neisseria gonorrhoeae strain with resistance to cefixime causing gonorrhoea treatment failure in Austria, 2011. Euro Surveill. 2011;16(43):pii=19998. [PubMed]
  • Unemo M, Golparian D, Nicholas R. et al. High-level cefixime- and ceftriaxone-resistant Neisseria gonorrhoeae in France: novel penA mosaic allele in a successful international clone causes treatment failure. Antimicrob Agents Chemother. 2012;56:1273–1280. doi: 10.1128/AAC.05760-11. [PMC free article] [PubMed] [Cross Ref]
  • Unemo M, Golparian D, Potočnik M. et al. Treatment failure of pharyngeal gonorrhoea with internationally recommended first-line ceftriaxone verified in Slovenia, September 2011. Euro Surveill. 2012;17(25):pii=20200. [PubMed]
  • Yokoi S, Deguchi T, Ozawa T. et al. Threat to cefixime treatment of gonorrhea. Emerg Infect Dis. 2007;13:1275–1277. [PMC free article] [PubMed]
  • Whiley DM, Goire N, Lahra MM. et al. The ticking time bomb: escalating antibiotic resistance in Neisseria gonorrhoeae is a public health disaster in waiting. J Antimicrob Chemother. 2012;67:2059–2061. doi: 10.1093/jac/dks188. [PubMed] [Cross Ref]
  • Ndowa F, Lusti-Narasimhan M, Unemo M. The serious threat of multidrug-resistant and untreatable gonorrhoea: the pressing need for global action to control the spread of antimicrobial resistance, and mitigate the impact on sexual and reproductive health. Sex Transm Infect. 2012;88:317–318. doi: 10.1136/sextrans-2012-050674. [PubMed] [Cross Ref]
  • European Centre for Disease Prevention and Control (ECDC) Response plan to control and manage the threat of multidrug-resistant gonorrhoea in Europe. Stockholm: ECDC; 2012. pp. 1–23. Available at: http://www.ecdc.europa.eu/en/publications/Publications/1206-ECDC-MDR-gonorrhoea-response-plan.pdf (Accessed: December 20, 2012)
  • Centers for Disease Control and Prevention (CDC) Cephalosporin-resistant Neisseria gonorrhoeae public health response plan. 2012. pp. 1–43. Available at: http://www.cdc.gov/std/gonorrhea/default.htm (Accessed: December 20, 2012)
  • Zhao S, Duncan M, Tomberg J. et al. Genetics of chromosomally mediated intermediate resistance to ceftriaxone and cefixime in Neisseria gonorrhoeae. Antimicrob Agents Chemother. 2009;53:3744–3751. doi: 10.1128/AAC.00304-09. [PMC free article] [PubMed] [Cross Ref]
  • Tomberg J, Unemo M, Davies C. et al. Molecular and structural analysis of mosaic variants of penicillin-binding protein 2 conferring decreased susceptibility to expanded-spectrum cephalosporins in Neisseria gonorrhoeae: role of epistatic mutations. Biochemistry. 2010;49:8062–8070. doi: 10.1021/bi101167x. [PMC free article] [PubMed] [Cross Ref]
  • Lewis DA. The Gonococcus fights back: is this time a knock out? Sex Transm Infect. 2010;86:415–421. doi: 10.1136/sti.2010.042648. [PubMed] [Cross Ref]
  • Ohneck EA, Zalucki YM, Johnson PJ. et al. A novel mechanism of high-level, broad-spectrum antibiotic resistance caused by a single base pair change in Neisseria gonorrhoeae. MBio. 2011;20:2(5). [PMC free article] [PubMed]
  • Unemo M, Fasth O, Fredlund H. et al. Phenotypic and genotypic characterization of the 2008 WHO Neisseria gonorrhoeae reference strain panel for use in global quality assurance and quality control of gonococcal antimicrobial resistance (AMR) surveillance for public health purposes. J Antimicrob Chemother. 2009;63:1142–1151. doi: 10.1093/jac/dkp098. [PubMed] [Cross Ref]
  • Bala M, Ray K, Kumari S. Alarming increase in ciprofloxacin and penicillin resistant Neisseria gonorrhoeae isolates in New Delhi, India. Sex Transm Dis. 2003;30:523–525. doi: 10.1097/00007435-200306000-00010. [PubMed] [Cross Ref]
  • Bala M, Ray K, Salhan S. First case of spectinomycin resistant Neisseria gonorrhoeae isolate in New Delhi, India. Sex Transm Infect. 2005;81:186–187. doi: 10.1136/sti.2004.011569. [PMC free article] [PubMed] [Cross Ref]
  • Bala M, Ray K, Gupta SM. et al. Changing trends of antimicrobial susceptibility patterns of Neisseria gonorrhoeae in India and the emergence of ceftriaxone less susceptible N. gonorrhoeae strains. J Antimicrob Chemother. 2007;60:582–586. doi: 10.1093/jac/dkm238. [PubMed] [Cross Ref]
  • Ray K, Bala M, Kumari S. et al. Antimicrobial resistance of Neisseria gonorrhoeae in selected World Health Organization Southeast Asia Region countries: an overview. Sex Transm Dis. 2005;32:178–184. doi: 10.1097/01.olq.0000154490.40381.15. [PubMed] [Cross Ref]
  • Jabeen K, Nizamuddin S, Irfan S. et al. Increasing trend of resistance to penicillin, tetracycline, and fluoroquinolone resistance in Neisseria gonorrhoeae from Pakistan (1992–2009) J Trop Med. 2011;2011:960501. [PMC free article] [PubMed]
  • Bala M. Characterization of profile of multidrug-resistant Neisseria gonorrhoeae using old and new definitions in India over a decade: 2000–2009. Sex Transm Dis. 2011;38:1056–1058. doi: 10.1097/OLQ.0b013e31822e6361. [PubMed] [Cross Ref]
  • Bala M. Antimicrobial resistance in Neisseria gonorrhoeae in South-East Asia. Regional Health Forum. 2011;15:63–73. Available at: http://www.searo.who.int/LinkFiles/Regional_Health_Forum_RHF_Vol_15_No_1.pdf (Accessed: December 20, 2012)
  • Kulkarni S, Bala M, Sane S. et al. Mutations in the gyrA and parC genes of quinolone-resistant Neisseria gonorrhoeae isolates in India. Int J Antimicrob Agents. 2012;40:549–553. doi: 10.1016/j.ijantimicag.2012.08.007. [PubMed] [Cross Ref]
  • Unemo M, Dillon JA. Review and international recommendation of methods for typing Neisseria gonorrhoeae isolates and their implications for improved knowledge of gonococcal epidemiology, treatment, and biology. Clin Microbiol Rev. 2011;24:447–458. doi: 10.1128/CMR.00040-10. [PMC free article] [PubMed] [Cross Ref]
  • Martin IM, Ison CA, Aanensen DM. et al. Rapid sequence-based identification of gonococcal transmission clusters in a large metropolitan area. J Infect Dis. 2004;189:1497–1505. doi: 10.1086/383047. [PubMed] [Cross Ref]
  • Agarwal SK, Deb M, Prakash K. et al. Auxotyping of Neisseria gonorrhoeae as an additional epidemiological marker. Indian J Med Res. 1992;95:227–229. [PubMed]
  • Divekar AA, Gogate AS, Shivkar LK. Association between auxotypes, serotypes and antibiotic susceptibilities of Neisseria gonorrhoeae isolated from women in Mumbai (formerly Bombay), India. Sex Transm Dis. 1999;26:358–363. doi: 10.1097/00007435-199907000-00009. [PubMed] [Cross Ref]
  • Khaki P, Bhalla P, Sharma P. et al. Epidemiological analysis of Neisseria gonorrhoeae isolates by antimicrobial susceptibility testing, auxotyping and serotyping. Indian J Med Microbiol. 2007;25:225–229. doi: 10.4103/0255-0857.34763. [PubMed] [Cross Ref]
  • Kulkarni S, Bala M, Risbud A. Antimicrobial susceptibility testing, auxotyping, and serotyping of Neisseria gonorrhoeae strains isolated in India. Sex Transm Dis. 2012;39:188–190. doi: 10.1097/OLQ.0b013e3182389a24. [PubMed] [Cross Ref]
  • Nizamuddin S, Jabeen K, Zafar A. Evaluation of predominant Neisseria gonorrhoeae strain types and its correlation with fluoroquinolone resistance in Pakistan. J Pak Med Assoc. 2011;61:446–449. [PubMed]
  • Bindu MP, Rajendran P, Thyagarajan SP. et al. Molecular typing of Neisseria gonorrhoeae from hospital and community isolates by restriction fragment length polymorphism. Int J STD AIDS. 2002;13:124–130. doi: 10.1258/0956462021924767. [PubMed] [Cross Ref]
  • Khaki P, Bhalla P, Fayaz AM. et al. Molecular typing of Neisseria gonorrhoeae isolates by Opa-typing and ribotyping in New Delhi, India. Int J Microbiol. 2009;2009:934823. [PMC free article] [PubMed]
  • Unemo M, Sjöstrand A, Akhras M. et al. Molecular characterization of Neisseria gonorrhoeae identifies transmission and resistance of one ciprofloxacin-resistant strain. APMIS. 2007;115:231–241. doi: 10.1111/j.1600-0463.2007.apm_487.x. [PMC free article] [PubMed] [Cross Ref]
  • Sethi S, Sharma D, Mehta SD. et al. Emergence of ciprofloxacin resistant Neisseria gonorrhoeae in north India. Indian J Med Res. 2006;123:707–710. [PubMed]
  • Brhlikova P, Harper I, Jeffery R. et al. Trust and the regulation of pharmaceuticals: South Asia in a globalised world. Global Health. 2011;7:10. doi: 10.1186/1744-8603-7-10. [PMC free article] [PubMed] [Cross Ref]
  • Bignell C. 2009 European (IUSTI/WHO) guideline on the diagnosis and treatment of gonorrhoea in adults. Int J STD AIDS. 2009;20:453–457. doi: 10.1258/ijsa.2009.009160. [PubMed] [Cross Ref]
  • Centers for Disease Control and Prevention (CDC) Update to CDC’s sexually transmitted diseases treatment guidelines, 2006: Fluoroquinolones no longer recommended for treatment of gonococcal infections. MMWR Morb Mortal Wkly Rep. 2007;56:332–336. [PubMed]
  • Centers for Disease Control and Prevention (CDC) Sexually Transmitted Diseases Treatment Guidelines, 2010. Atlanta, GA: CDC; 2010. Available at: http://www.cdc.gov/std/treatment/2010/STD-Treatment-2010-RR5912.pdf (Accessed December 20, 2012)
  • Centers for Disease Control and Prevention (CDC) Update to CDC’s Sexually Transmitted Diseases Treatment Guidelines, 2010: Oral cephalosporins no longer a recommended treatment for gonococcal infections. MMWR Morb Mortal Wkly Rep. 2012;61:590–594. [PubMed]
  • NACO. Operational guidelines for programme managers and service providers for strengthening STI/RTI services. NACO; 2007. Available at: http://nacoonline.org/upload/Policies%20&%20Guidelines/21,%20Operational%20Guidelines%20STI%20RTI.pdf (Accessed December 20, 2012)
  • Chisholm SA, Dave J, Ison CA. High-level azithromycin resistance occurs in Neisseria gonorrhoeae as a result of a single point mutation in the 23S rRNA genes. Antimicrob Agents Chemother. 2010;54:3812–3816. doi: 10.1128/AAC.00309-10. [PMC free article] [PubMed] [Cross Ref]
  • Galarza PG, Abad R, Canigia LF. et al. New mutation in 23S rRNA gene associated with high level of azithromycin resistance in Neisseria gonorrhoeae. Antimicrob Agents Chemother. 2010;54:1652–1653. doi: 10.1128/AAC.01506-09. [PMC free article] [PubMed] [Cross Ref]
  • Katz AR, Komeya AY, Soge OO. et al. Neisseria gonorrhoeae with high-level resistance to azithromycin: case report of the first isolate identified in the United States. Clin Infect Dis. 2012;54:841–843. doi: 10.1093/cid/cir929. [PubMed] [Cross Ref]
  • Palmer HM, Young H, Winter A. et al. Emergence and spread of azithromycin-resistant Neisseria gonorrhoeae in Scotland. J Antimicrob Chemother. 2008;62:490–494. doi: 10.1093/jac/dkn235. [PubMed] [Cross Ref]
  • Starnino S, Stefanelli P. Neisseria gonorrhoeae Italian Study Group I. Azithromycin-resistant Neisseria gonorrhoeae strains recently isolated in Italy. J Antimicrob Chemother. 2009;63:1200–1204. doi: 10.1093/jac/dkp118. [PubMed] [Cross Ref]
  • Golparian D, Hellmark B, Fredlund H. et al. Emergence, spread and characteristics of Neisseria gonorrhoeae isolates with in vitro decreased susceptibility and resistance to extended-spectrum cephalosporins in Sweden. Sex Transm Infect. 2010;86:454–460. doi: 10.1136/sti.2010.045377. [PubMed] [Cross Ref]
  • Chisholm SA, Unemo M, Quaye N. et al. Molecular epidemiological typing within the European Gonococcal Antimicrobial Resistance Surveillance Programme reveals predominance of a multidrug-resistant clone. Euro Surveill. 2013;18(3):pii=20358. [PubMed]
  • Heymans R, Bruisten SM, Golparian D. et al. Clonally related Neisseria gonorrhoeae isolates with decreased susceptibility to the extended-spectrum cephalosporin cefotaxime in Amsterdam, the Netherlands. Antimicrob Agents Chemother. 2012;56:1516–1522. doi: 10.1128/AAC.05481-11. [PMC free article] [PubMed] [Cross Ref]
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