Search tips
Search criteria 


Logo of jbcThe Journal of Biological Chemistry
J Biol Chem. 2013 February 1; 288(5): 3439–3448.
Published online 2012 December 14. doi:  10.1074/jbc.M112.431932
PMCID: PMC3561562

ATP Alone Triggers the Outward Facing Conformation of the Maltose ATP-binding Cassette Transporter*


The maltose transporter MalFGK2 is a study prototype for ABC importers. During catalysis, the MalFG membrane domain alternates between inward and outward facing conformations when the MalK dimer closes and hydrolyzes ATP. Because a rapid ATP hydrolysis depends on MalE and maltose, it has been proposed that closed liganded MalE facilitates the transition to the outward facing conformation. Here we find that, in contrast to the expected, ATP is sufficient for the closure of MalK and for the conversion of MalFG to the outward facing state. The outward facing transporter binds MalE with nanomolar affinity, yet neither MalE nor maltose is necessary or facilitates the transition. Thus, the rapid hydrolysis of ATP observed in the presence of MalE and maltose is not because closed liganded MalE accelerates the formation of the outward facing conformation. These findings have fundamental implications for the description of the transport reaction.

Keywords: ABC Transporter, ATPases, Enzyme Kinetics, Membrane Proteins, Transport, Nanodiscs


ATP-binding cassette (ABC)3 transporters are found both in prokaryotes and eukaryotes and transport a wide variety of substrates in and out of cells. The understanding of their mode of operation is relevant for medically important processes such as multidrug resistance, chloride conductance, cholesterol transport, and surface-antigen presentation (1). In bacteria, the maltose transporter has been studied for several decades as the prototype of ABC importers (24). The transporter is comprised of a cytosolic homodimeric ABC domain MalK bound to a membrane domain heterodimer, MalFG. On its periplasmic side, the transporter associates with the substrate-binding protein MalE that acts as a receptor for maltose.

Major progress in the description of ABC transporters has been achieved by x-ray crystallography (57). In the structure of MalFGK2 without ligands, the sites for ATP binding on the MalK dimer are separated, and the MalFG membrane domain forms a cavity that is exposed to the cytosol (inward facing state) (8). In the structure obtained in the presence of MalE and nonhydrolyzable ATP analogs, the ATP-binding sites are bound together, whereas the MalFG cavity opens toward the periplasm (outward facing state) (6, 9). Together, these snapshots of the transporter structure have provided the molecular framework to explain how ATP binding and hydrolysis is coupled to membrane transport, the so-called alternating access mechanism. However, what triggers the transport reaction, why MalE stimulates ATP hydrolysis even in the absence of maltose, and how maltose is transferred from MalE to MalFG remain unclear.

MalE consists of two lobes with the maltose-binding site located at the interface. When the sugar binds, the two lobes close and eventually capture maltose with a high affinity (conformation termed closed liganded MalE) (10, 11). Because the ATPase activity of the transporter is maximal only in the presence of MalE and maltose, it has been proposed that closed liganded MalE binds the transporter and triggers ATP hydrolysis (12, 13). The x-ray structures were interpreted with this assumption in mind, leading to the model in Fig. 1A (2). In that model, the binding of closed liganded MalE to the transporter induces the pairing of MalK. The pairing of MalK triggers the MalFG outward facing conformation, which in turn forces the opening of MalE and the release of maltose. Upon ATP hydrolysis, the transporter returns to the inward facing state, and maltose enters the cytosol. In support of the model, an EPR spectroscopy study reported that the MalK nucleotide-binding interface closes only in the presence of MalE and ATP (14). A partial closure of the MalK interface was also observed when MalFGK2 was co-crystallized with closed liganded MalE. It was concluded that MalE facilitates the pairing of MalK and therefore hydrolysis of ATP (15). However, despite the evidence and the prevalence of the model, we recently reported that closed liganded MalE does not bind the transporter but dissociates when MalE captures maltose. We also reported that open state MalE binds with nanomolar affinity to the outward facing transporter and creates a low affinity receptor for maltose (16). These data, difficult to reconciliate with the former model, led us to propose a new model (Fig. 1B and legend for details about the binding affinities).

Two opposed models for maltose transport. A, in the conventional model, closed liganded MalE triggers the outward facing conformation. MalE binds maltose (Kd = ~2 μm) and then associates with the inward facing transporter (Kd > ...

Here, we further investigate the effect of MalE, maltose, and ATP on the conformation of MalFGK2. Indeed, an important and central question is the mechanism leading to the outward facing conformation. The transition depends on MalE and maltose in the former model, but not in the newer model. Using cross-linking, co-sedimentation analysis, native gels, and fluorescence assays, we show that MalE and closed liganded MalE do not facilitate the closure of MalK dimer, nor the transition of MalFG to the outward facing state. Instead, we find that ATP alone is sufficient. Because MalE does not facilitate the binding of ATP to the transporter (16, 17), nor the outward facing conformation, the results imply that MalE must stimulate a step occurring during the return of the transporter to the inward facing state.



The detergent n-dodecyl-β-d-maltoside (DDM) was purchased from Anatrace. The lipids 1,2-dioleoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) were purchased from Avanti Polar Lipids. Superdex 200 10/300 GL, Resource 15Q, Sephadex G-25, and Ni2+-NTA chelating Sepharose columns were obtained from GE Healthcare. BioBeads were purchased from Bio-Rad. N-(1-Pyrene)-maleimide was obtained from AnaSpec. All other chemicals, including bis-maleimidoethane (BMOE) and N-ethylmaleimide, were obtained from Sigma. MalE and His-tagged MalFGK2 were purified as previously described (16), using Escherichia coli BL21 (DE3) carrying plasmids pBAD33-MalE or pBAD22-MalFGKhis.

Reconstitution of MalFGK2 in Nanodiscs and Proteoliposomes

The membrane scaffold protein MSP1D1 was obtained from the Sligar laboratory (18). The lipids 1,2-dioleoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) were mixed at a ratio of 7:3, dissolved in chloroform, and dried under a steam of nitrogen. The lipids were resuspended in TSG buffer (50 mm Tris-HCl, pH 8.0, 100 mm NaCl, 10% glycerol) containing 0.2% DDM. The MalFGK2 complex, the membrane scaffold proteins, and the solubilized lipids were mixed together at a protein:MSP:lipid ratio of 1:3:60 in TSG buffer containing 0.08% DDM. Detergent was removed with BioBeads (one-third volume) and gentle shaking (overnight, 4 °C). BioBeads were removed by sedimentation, and the resulting nanodiscs were purified through Superdex 200 10/30 gel filtration equilibrated in TSG buffer. Proteoliposomes were prepared at a protein:lipid ratio of 1:2000 in TSG buffer containing 0.08% DDM. Detergent was removed using BioBeads as for nanodiscs reconstitution. The proteoliposomes were harvested by centrifugation (100,000 × g, 1h, 4 °C) and resuspended in 20 mm Tris-HCl, pH 8.0. The orientation of the complex reconstituted in proteoliposomes was determined by the labeling of residue MalFT177C (in background MalKC40S) with 5-iodoacetamide fluorescein (5-IAF). Proteolipsomes (2 μm) were incubated with 5-IAF (10 μm) in 20 mm Tris-HCl, pH 8.0, 1 mm MgCl2 supplemented with or without 1% DDM. The reaction was stopped with DTT (2 mm), and unreacted 5-IAF was removed by gel filtration (Sephadex G-25). Proteoliposomes were extruded through a 100-nm polycarbonate filter before use.

Maltose Transport Assay

Proteoliposomes were reconstituted in R buffer (50 mm sodium phosphate, pH 6.5, 1 mm MgCl2, 5 mm ATP). The transport reaction was carried out with 10 μm MalE, 0.5 μm MalFGK2, and 20 μm [14C]maltose (57 μCi/μmol). At the indicated time, samples (50 μl) were removed and diluted into 1 ml of ice-cold 50 mm sodium phosphate buffer containing 10 mm maltose followed by filtration through a 0.22-μm nitrocellulose Millipore filter and scintillation counting. The number of cpm were converted into nmol using known amounts of [14C]maltose.

Cysteine Cross-linking Experiments

Cross-linking reactions were performed in 50 mm Tris-HCl, pH 8.0, 10 mm MgCl2 containing 0.5 μm MalFGK2 proteoliposomes mixed with 1 mm ATP, 10 μm MalE, and 1 mm maltose as indicated. The samples were incubated with 50 μm BMOE (10 min at room temperature) and then treated with N-ethylmaleimide (5 mm) before analysis by SDS-PAGE.

Fluorescence Labeling and Spectroscopy

The dye N-(1-pyrene)-maleimide was used to label the MalFGK2 cysteine mutants. Briefly, the purified MalFGK2 complex was incubated with 0.5 mm DTT (30 min on ice) and passed through a G25 column. The labeling reaction was then performed by mixing MalFGK2 and N-(1-pyrene)-maleimide at a molar ratio of 1:10 in TSG buffer containing 0.01% DDM and 1 mm TCEP for 2 h at room temperature. The unreacted N-(1-pyrene)-maleimide was removed by Superdex 200 10/30 gel filtration. The purified and labeled complex was reconstituted in proteoliposomes as described above. The labeling efficiency was determined by absorbance spectroscopy (343 nm) using an extinction co-efficient of 40,000 cm−1 m−1. A typical ratio was between 1.2 and 1.4. Because there are two cysteine substitutions per MalFGK2 variant, this is equal to ~60–70% labeling efficiency. The pyrene-labeled MalFGK2 variants retained ~80% ATPase activity and 70% transport activity of wild type MalFGK2 (see Table 2). The fluorescent measurements were carried out at 25 °C in FL buffer (50 mm Tris-HCl, pH 8.0, 5 mm MgCl2) on a Cary Eclipse spectrofluorometer. The fluorescence spectrums were recorded from 365 to 520 nm with excitation wavelength at 345 nm (5-nm slide width). The binding of MalE to MalFGK2 was measured by fluorescence quenching assay, using MalE-31C labeled with the maleimide derivative ATTO-655 (Atto-Tech), as previously described (16). The fluorescent measurements were carried out at 25 °C in FL buffer.

ATPase activity of the pyrene-labeled transporters reconstituted in proteoliposomes

Other Methods

The transporter ATPase activity was determined by measuring the release of inorganic phosphate using photo colorimetric method (16). Linear gradient native gel electrophoresis, sedimentation assay, and protein iodination were performed as described (16, 19).


ATP Controls the Outward Facing Conformation of the Transporter

Cysteine residues were introduced at diagnostic positions on MalK, MalF, and MalG, so that sulfhydryl-based cross-linking methods could be employed to monitor the conformation of the transporter in the lipid bilayer (Fig. 2A). The cysteine residues were introduced into a complex mutated at the endogenous Cys-40 of MalK because this position naturally reacts with maleimide reagents (data not shown). According to the crystallographic data (8, 9), the distance between the periplasmic positions MalF446/MalG234 increases from ~9 to ~18 Å during the conformational transition from the inward facing state to the outward facing state. Meanwhile, the distance between the cytosolic positions MalK83/MalK83 and MalF396/MalG185 decreases from ~ 21 to ~8 Å. We thus employed the homobifunctional cross-linker BMOE with a spacer arm of 8 Å to monitor these conformational changes (Fig. 2).

ATP triggers the transporter outward facing conformation. A, the asterisks indicate the location of the cysteine residues introduced in MalF, MalG, and MalK and their relative positions in the inward and outward facing conformations. B, orientation of ...

We note that the C-terminal tail of MalG in the crystal structure is located near the MalK dimer interface and may potentially interfere with the formation of the cross-link MalK83/MalK83. However, as reported by others, an efficient ATP-dependent cross-linking occurs with this pair (and also with MalK85/MalK85), showing that the C-terminal tail of MalG is sufficiently mobile in solution to allow cross-link formation (20, 21). We also considered the possibility that MalFGK2 becomes inaccessible to MalE when the transporter is reconstituted in liposomes. This was ruled out because (i) the transporter was functional (Table 1), and its activity was dependent on MalE and maltose, (ii) the sedimentation of MalE with the proteoliposome was dependent on MalFGK2 and nucleotides (Fig. 3, A and C), and (iii) the thiol-reactive membrane-impermeable reagent 5-IAF labeled the periplasmic side of the complex with at least 50% efficiency (Fig. 2B).

The affinity of MalE for the transporter in nanodiscs determined by fluorescence quenching assay as previously described (16)
ATP controls the binding of MalE to the transporter. A, the binding of MalE to MalFGK2 in proteoliposomes was analyzed by co-sedimentation assay. [125I]MalE (~10,000 cpm, 10 μm) was incubated with MalFGK2 proteoliposomes (2 μm ...

We then probed what the transporter conformation is in the membrane. In the absence of ligands (apo state), there was only a little cross-link formation with the pairs MalK83/MalK83 and MalF396/MalG185 (pairs located in the cytosolic side of the membrane), whereas cross-links readily formed with the pair MalF446/MalG234 (located on the periplasmic side) (Fig. 2, C–E). These results were expected because the transporter resides naturally in the inward facing state in the absence of ligands (8). In the presence of ATP, however, a predominant cross-link appeared with the pairs MalK83/MalK83 and MalF396/MalG185, whereas the cross-link of MalF446/MalG234 was decreased by ~90%, as determined by densitometry analysis (data not shown). The binding of ATP, and not its hydrolysis, was responsible for this conformational change because ATPγS produced the same effect (Fig. 2F). Strikingly, the addition of MalE with or without maltose did not cause any differences in the cross-linking efficiencies (Fig. 2, C–F). Together, these data provided strong preliminary evidence that ATP on its own controls the transition of the transporter from inward to outward facing state.

ATP Controls the Binding of MalE to the Transporter

We reported recently that MalE binds with nanomolar affinity to the outward facing transporter (16). Because ATP alone appears sufficient for the transition to the outward facing conformation, we hypothesized that ATP alone should also control the binding of MalE to the transporter. Accordingly, the stabilization of MalFGK2 in the outward facing state with ATP plus vanadate allowed an efficient co-sedimentation of MalE with the transporter (Fig. 3A, lanes 2). In the absence of vanadate, the binding of MalE was weak because the transporter hydrolyzes ATP and returns to the inward facing conformation, which has no affinity for MalE. Accordingly, when the mutants MalK83/MalK83 and MalF396/MalG185 were treated with ATP plus BMOE to stabilize the transporter in the outward facing state, the binding of MalE was as efficient as with ATP plus vanadate (Fig. 3A, compare lanes 2 with lanes 4). In contrast, when the transporter was locked in the inward facing state with the pair MalF446/MalG234, the binding of MalE was at background levels, even in the presence of ATP and vanadate (Fig. 3A, right panel, lane 4). The results therefore confirm that ATP controls the transition from inward to outward facing state, which in turn controls the binding of MalE to the transporter.

We next employed a fluorescence-based assay to determine the binding affinity of MalE to MalFGK2 in the inward and outward facing states. We reconstituted the transporter in nanodisc because the system is well suited for spectroscopic analysis (22). First, we confirmed on native gel that MalE binds to the transporter with the same characteristics in nanodiscs as in proteoliposomes (Fig. 3, compare B with A; ATP dependence). We then titrated the fluorescent-labeled MalE with an increasing amount of transporter (Fig. 4). The data showed that MalE binds with a high affinity (Kd = ~50–80 nm) only when the transporter is stabilized in the outward facing conformation with ATP-vanadate or ATP-BMOE (binding data and standard deviations reported in Table 1). In contrast, in the absence of nucleotides, the binding affinity of MalE for the transporter was ~7 μm and increased furthermore to ~45 μm when MalE was liganded with maltose (Table 1 and Fig. 3C). Because the transporter might spontaneously acquire its outward facing conformation even in the absence of ATP, the affinity of liganded MalE to the inward facing state may in fact be lower than 45 μm. Indeed, the interaction of maltose-bound MalE was virtually null when the transporter was stabilized in the inward facing state using the cysteine pair MalF446/MalG234 (Fig. 4D). The reaction was not saturable, and therefore the affinity could not be determined, in agreement with earlier binding assays in proteoliposomes (23). We concluded that ATP controls the transporter outward facing conformation and thereby controls the high affinity binding of MalE to the transporter.

Equilibrium titration of MalE binding to MalFGK2 in nanodiscs. The MalFGK2 complex in nanodiscs was incubated with ATP (1 mm), BMOE (50 μm), and vanadate (10 μm) as indicated (10 min at room temperature). The nanodiscs were repurified ...

MalE Does Not Facilitate the Transition to the Outward Facing Conformation

As an additional way to probe the transporter conformational state and to explore the transition kinetics, we employed the fluorescent dye N-(1-pyrene) maleimide. When attached to a protein thiol, the dye produces two distinct emission peaks at 375 and 395 nm. When two pyrene-thiols interact within 6–10 Å distance, they form excited state dimers (excimers) that emit at longer wavelengths than the lone excited fluorophore (24, 25). The transporter labeled with the pyrene probe at the cysteine positions described above retained ~80% ATPase activity and 70–80% maltose transport activity as compared with the wild type complex (Table 2). The fluorescence spectra were recorded between 365 and 520 nm to monitor the conformational changes in the presence of ATP, MalE, and maltose (Fig. 5, red traces), compared with without ligands (black traces). With the pairs MalK83/MalK83 and MalF396/MalG185, which monitor the conformational changes on the cytosolic side of the membrane, ATP decreased the monomeric emissions but increased the excimer emissions (Fig. 5, A and B). The reverse was observed with the pair MalF446/MalG234. This was expected because the pair monitors the conformational change on the opposite side of the membrane (Fig. 5C). In contrast, MalE and maltose had no effect on the monomeric and excimer emissions, in agreement with the conclusion that ATP alone supports the outward facing conformation of the transporter. Furthermore, we observed that MalE and maltose had no effect on the kinetics of the conformational transition (Fig. 6). These time-resolved experiments showed that liganded MalE is unable to modify the kinetics of MalK closure, even though we employed a cysteine pair that produced the highest fluorescence signal difference (i.e., MalK83/MalK83). We therefore conclude that MalE is not required, nor does it facilitate the closure of the nucleotide-binding domain in the intact and functional maltose ABC transporter.

Neither MalE nor maltose facilitates the transition to the outward facing state. The steady state fluorescence emission spectra of pyrene-labeled MalFGK2 recorded in the absence (black traces) or presence of ATP, MalE, and maltose (red traces) using the ...
Transition kinetics toward the outward facing conformation. The time course fluorescence emission spectra for the pair MalK83/MalK83 were monitored after the addition of ATP, MalE, and maltose as indicated. The spectra were recorded at 375 nm to monitor ...


We report that ATP alone is sufficient for the closure of the MalK interface and for the conversion of MalFG to the outward facing conformation. This conclusion is based on cysteine cross-links and pyrene-based fluorescence of probes placed at diagnostic positions on the transporter. The cysteine substitutions and the pyrene label affect modestly the ATPase activity of the transporter, and do not affect its capacity to import maltose (i.e., 70–80% activity of the wild type; Table 2). The conclusion we reach is in fact not surprising but rather in agreement with other studies reporting that ATP drives the dimerization of MalK in the membrane or in solution (2628). The conclusion is also consistent with the x-ray structures that showed the rigid body rotation of MalFG upon closure of MalK (8). That ATP alone regulates the conformation of the transporter has also been reported for other type I and type II importers (ModBC and BtuCD, respectively), as well as for ABC exporters (7, 2932).

Our results, however, disagree with the accepted notion that closed liganded MalE facilitates the closure of the MalK dimer. This mechanism was proposed (and perhaps intuitively accepted) because a rapid ATP hydrolysis activity occurs only in the presence of MalE and maltose. To the best of our knowledge, this model has been directly tested by EPR spectroscopy only (14). Why the study concluded that MalK closure strictly depends on MalE is puzzling. The spin label reduced the MalK ATPase activity by 2-fold (from 2,100 to 1,150 nmol/min/mg), yet the transporter was still active. The spin labels were, however, placed at two different positions on the same MalK subunit, giving rise to four different possible ways of spin interactions. Most importantly, the EPR distance measurements were performed in detergent solution, whereas the transporter ATPase is ~100-fold higher than in the membrane and poorly dependent on MalE and maltose (16). The sensitivity of EPR in the membrane environment is indeed problematic because of the large spin decay inherent to intermolecular dipolar interactions (33), and accordingly, the authors could not report the interspin distances with the transporter reconstituted in liposomes. Nevertheless, this pioneering work showed that ATP is essential for the pairing of MalK, and this conclusion is consistent with our results.

Our results also contrast with the interpretation of the crystal structure that captured closed liganded MalE with the transporter in a semi-closed conformation (15). It was concluded that closed liganded MalE binds to MalFG and induces the preclosure of MalK and thus ATP hydrolysis. Our pyrene-based fluorescence experiments rather show that the kinetics of MalK pairing is the same, whether MalE and maltose are present or not (Fig. 5). In addition, we find that the binding of closed liganded MalE to the inward facing transporter is quasi-null in solution (Kd > 45 μm; Table 1). In contrast, the binding of MalE to the outward facing transporter occurs with nanomolar affinity (Kd = ~60 nm; Table 1). Clearly, a far more dominant interaction is taking place between ATP-bound outward facing MalFGK2 and unliganded open state MalE. Given the high concentration of ATP in the cytosol (~1 mm), we think this mode of interaction represents the resting state of the transporter. This view is supported by an in vivo cross-linking analysis, which reported that MalE is bound to the transporter even in the absence of maltose (34). This view is also consistent with our conclusion that transporter-bound MalE forms a receptor for maltose (16). In contrast, a model involving the low affinity interaction between closed liganded MalE and MalFGK2 (Kd > 45 μm), as a way to deliver the sugar and activate the transporter, seems unsustainable because the concentration of periplasmic MalE decreases further as the environmental maltose concentration decreases.

An obvious question raised by this work is how MalE stimulates the MalK ATPase. The co-crystal of closed liganded MalE with MalFGK2 has led to the proposal that MalE lowers the energy barrier for ATP hydrolysis (15). However, it cannot be excluded that the binding of MalE to the outward facing transporter instead stimulates the release of ADP and/or Pi and thus the ATP turnover rate (and furthermore in the presence of maltose). Earlier studies suggested that ATP product release may coincide with the reorientation of MalFGK2 to the inward facing conformation (14). For the mammalian P-glycoprotein, it was proposed that phosphate release is coupled to transport (35). Further work should therefore address what is the rate-limiting step during maltose transport. Our current work shows that the affinity of the transporter for ATP is not increased with MalE (16) and that ATP alone is sufficient to convert the transporter to the outward facing conformation. Together, these results imply that MalE must stimulate a step that occurs when the transporter returns to the inward facing conformation. This conclusion is compatible with the autoregulation model presented in Fig. 1B.

*This work was supported by an infrastructure grant from the Canadian Foundation for Innovation.

3The abbreviations used are:

ATP-binding cassette
5-iodoacetamide fluorescein.


1. Rees D. C., Johnson E., Lewinson O. (2009) ABC transporters. The power to change. Nat. Rev. Mol. Cell Biol. 10, 218–227 [PMC free article] [PubMed]
2. Davidson A. L., Dassa E., Orelle C., Chen J. (2008) Structure, function, and evolution of bacterial ATP-binding cassette systems. Microbiol. Mol. Biol. Rev. 72, 317–364 [PMC free article] [PubMed]
3. Shilton B. H. (2008) The dynamics of the MBP-MalFGK2 interaction. A prototype for binding protein dependent ABC-transporter systems. Biochim. Biophys. Acta 1778, 1772–1780 [PubMed]
4. Bordignon E., Grote M., Schneider E. (2010) The maltose ATP-binding cassette transporter in the 21st century. Towards a structural dynamic perspective on its mode of action. Mol. Microbiol. 77, 1354–1366 [PubMed]
5. Procko E., O'Mara M. L., Bennett W. F., Tieleman D. P., Gaudet R. (2009) The mechanism of ABC transporters. General lessons from structural and functional studies of an antigenic peptide transporter. FASEB J. 23, 1287–1302 [PubMed]
6. Oldham M. L., Chen J. (2011) Snapshots of the maltose transporter during ATP hydrolysis. Proc. Natl. Acad. Sci. U.S.A. 108, 15152–15156 [PubMed]
7. Hollenstein K., Dawson R. J., Locher K. P. (2007) Structure and mechanism of ABC transporter proteins. Curr. Opin. Struct. Biol. 17, 412–418 [PubMed]
8. Khare D., Oldham M. L., Orelle C., Davidson A. L., Chen J. (2009) Alternating access in maltose transporter mediated by rigid-body rotations. Mol. Cell. 33, 528–536 [PMC free article] [PubMed]
9. Oldham M. L., Khare D., Quiocho F. A., Davidson A. L., Chen J. (2007) Crystal structure of a catalytic intermediate of the maltose transporter. Nature 450, 515–521 [PubMed]
10. Spurlino J. C., Lu G. Y., Quiocho F. A. (1991) The 2.3-A resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis. J. Biol. Chem. 266, 5202–5219 [PubMed]
11. Sharff A. J., Rodseth L. E., Spurlino J. C., Quiocho F. A. (1992) Crystallographic evidence of a large ligand-induced hinge-twist motion between the two domains of the maltodextrin binding protein involved in active transport and chemotaxis. Biochemistry 31, 10657–10663 [PubMed]
12. Davidson A. L., Shuman H. A., Nikaido H. (1992) Mechanism of maltose transport in Escherichia coli. Transmembrane signaling by periplasmic binding proteins. Proc. Natl. Acad. Sci. U.S.A. 89, 2360–2364 [PubMed]
13. Davidson A. L. (2002) Mechanism of coupling of transport to hydrolysis in bacterial ATP-binding cassette transporters. J. Bacteriol. 184, 1225–1233 [PMC free article] [PubMed]
14. Orelle C., Ayvaz T., Everly R. M., Klug C. S., Davidson A. L. (2008) Both maltose-binding protein and ATP are required for nucleotide-binding domain closure in the intact maltose ABC transporter. Proc. Natl. Acad. Sci. U.S.A. 105, 12837–12842 [PubMed]
15. Oldham M. L., Chen J. (2011) Crystal structure of the maltose transporter in a pretranslocation intermediate state. Science 332, 1202–1205 [PubMed]
16. Bao H., Duong F. (2012) Discovery of an auto-regulation mechanism for the maltose ABC transporter MalFGK2. PLoS One 7, e34836. [PMC free article] [PubMed]
17. Reich-Slotky R., Panagiotidis C., Reyes M., Shuman H. A. (2000) The detergent-soluble maltose transporter is activated by maltose binding protein and verapamil. J. Bacteriol. 182, 993–1000 [PMC free article] [PubMed]
18. Denisov I. G., Grinkova Y. V., Lazarides A. A., Sligar S. G. (2004) Directed self-assembly of monodisperse phospholipid bilayer Nanodiscs with controlled size. J. Am. Chem. Soc. 126, 3477–3487 [PubMed]
19. Dalal K., Duong F. (2010) Reconstitution of the SecY translocon in nanodiscs. Methods Mol. Biol. 619, 145–156 [PubMed]
20. Daus M. L., Grote M., Müller P., Doebber M., Herrmann A., Steinhoff H. J., Dassa E., Schneider E. (2007) ATP-driven MaIK dimer closure and reopening and conformational changes of the “EAA” motifs are crucial for function of the maltose ATP-binding cassette transporter (MaIFGK2). J. Biol. Chem. 282, 22387–22396 [PubMed]
21. Grote M., Bordignon E., Polyhach Y., Jeschke G., Steinhoff H. J., Schneider E. (2008) A comparative electron paramagnetic resonance study of the nucleotide-binding domains' catalytic cycle in the assembled maltose ATP-binding cassette importer. Biophys. J. 95, 2924–2938 [PubMed]
22. Bayburt T. H., Sligar S. G. (2010) Membrane protein assembly into Nanodiscs. FEBS Lett. 584, 1721–1727 [PMC free article] [PubMed]
23. Austermuhle M. I., Hall J. A., Klug C. S., Davidson A. L. (2004) Maltose-binding protein is open in the catalytic transition state for ATP hydrolysis during maltose transport. J. Biol. Chem. 279, 28243–28250 [PubMed]
24. Sahoo D., Narayanaswami V., Kay C. M., Ryan R. O. (2000) Pyrene excimer fluorescence. A spatially sensitive probe to monitor lipid-induced helical rearrangement of apolipophorin III. Biochemistry 39, 6594–6601 [PubMed]
25. Lehrer S. S. (1995) Pyrene excimer fluorescence as a probe of protein conformational change. Subcell. Biochem. 24, 115–132 [PubMed]
26. Hunke S., Mourez M., Jehanno M., Dassa E., Schneider E. (2000) ATP modulates subunit-subunit interactions in an ATP-binding cassette transporter (MalFGK2) determined by site-directed chemical cross-linking. J. Biol. Chem. 275, 15526–15534 [PubMed]
27. Daus M. L., Grote M., Müller P., Doebber M., Herrmann A., Steinhoff H. J., Dassa E., Schneider E. (2007) ATP-driven MalK dimer closure and reopening and conformational changes of the “EAA” motifs are crucial for function of the maltose ATP-binding cassette transporter (MalFGK2). J. Biol. Chem. 282, 22387–22396 [PubMed]
28. Smith P. C., Karpowich N., Millen L., Moody J. E., Rosen J., Thomas P. J., Hunt J. F. (2002) ATP binding to the motor domain from an ABC transporter drives formation of a nucleotide sandwich dimer. Mol. Cell 10, 139–149 [PMC free article] [PubMed]
29. Gerber S., Comellas-Bigler M., Goetz B. A., Locher K. P. (2008) Structural basis of trans-inhibition in a molybdate/tungstate ABC transporter. Science 321, 246–250 [PubMed]
30. Goetz B. A., Perozo E., Locher K. P. (2009) Distinct gate conformations of the ABC transporter BtuCD revealed by electron spin resonance spectroscopy and chemical cross-linking. FEBS Lett. 583, 266–270 [PubMed]
31. Joseph B., Jeschke G., Goetz B. A., Locher K. P., Bordignon E. (2011) Transmembrane gate movements in the type II ATP-binding cassette (ABC) importer BtuCD-F during nucleotide cycle. J. Biol. Chem. 286, 41008–41017 [PMC free article] [PubMed]
32. Locher K. P. (2009) Review. Structure and mechanism of ATP-binding cassette transporters. Philos. Trans. R. Soc. Lond. B Biol. Sci. 364, 239–245 [PMC free article] [PubMed]
33. McHaourab H. S., Steed P. R., Kazmier K. (2011) Toward the fourth dimension of membrane protein structure. Insight into dynamics from spin-labeling EPR spectroscopy. Structure 19, 1549–1561 [PMC free article] [PubMed]
34. Daus M. L., Berendt S., Wuttge S., Schneider E. (2007) Maltose binding protein (MalE) interacts with periplasmic loops P2 and P1 respectively of the MalFG subunits of the maltose ATP binding cassette transporter (MalFGK2) from Escherichia coli/Salmonella during the transport cycle. Mol. Microbiol. 66, 1107–1122 [PubMed]
35. Urbatsch I. L., Sankaran B., Bhagat S., Senior A. E. (1995) Both P-glycoprotein nucleotide-binding sites are catalytically active. J. Biol. Chem. 270, 26956–26961 [PubMed]

Articles from The Journal of Biological Chemistry are provided here courtesy of American Society for Biochemistry and Molecular Biology