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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Arch Dis Child. Author manuscript; available in PMC 2014 January 1.
Published in final edited form as:
PMCID: PMC3560933
NIHMSID: NIHMS437329

Season of Birth in a Nationwide Cohort of Coeliac Disease Patients

Benjamin Lebwohl, MD, MS,1,2 Peter HR Green, MD,1 Joseph A. Murray, MD,3 and Jonas F. Ludvigsson, MD, PhD2,4,*

Abstract

Background and objective

Genetic factors alone cannot explain the risk of developing coeliac disease (CD). Children born in summer months are likely to be weaned and introduced to gluten during winter when viral infections are more frequent. Earlier studies on birth season and CD are limited in sample size and results are contradictory.

Method

Case-control study. We used biopsy reports from all 28 Swedish pathology departments to identify individuals with CD, defined as small intestinal villous atrophy (n=29,096). The government agency Statistics Sweden then identified 144,522 controls matched for gender, age, calendar year and county. Through conditional logistic regression we examined the association between summer birth (March-August) and later CD diagnosis (outcome measure).

Results

Some 54.10% of individuals with CD vs. 52.75% of controls were born in the summer months. Summer birth was hence associated with a small increased risk of later CD (Odds ratio: 1.06; 95%CI=1.03–1.08; p<0.0001). Stratifying CD patients according to age at diagnosis, we found the highest OR in those diagnosed before age 2 years (OR=1.17; 95%CI=1.10–1.26), while summer birth was not associated with a CD diagnosis in later childhood (age 2–18 years: OR=1.02; 95%CI=0.97–1.08), but had a marginal effect on the risk of CD in adulthood (age ≥18years: OR=1.04; 95%CI=1.01–1.07).

Conclusions

In this study, summer birth was associated with an increased risk of later CD, but the excess risk was small, and general infectious disease exposure early in life is unlikely to be a major cause of CD.

Keywords: celiac, coeliac, epidemiology, risk factors, seasons, viral infection

INTRODUCTION

Coeliac disease (CD) occurs in approximately 1–2% of the Western population[1] and is characterized by small intestinal inflammation with villous atrophy. CD is strongly associated with Human Leukocyte Antigen (HLA) DQ2.5 (DQA1*05/DQB1*02)[2] and DQ8 (DQA1*03,DQB1*03:02)[3]. Although the CD concordance rate is high in monozygotic twins (above 70%),[4] HLA alone is not sufficient to predict future CD. Only one out of thirty DQ2+ individuals develop CD.[5] In recent years, a number of other potentially important genes have been linked to CD. Still, these genetic factors cannot entirely determine the risk of developing CD, nor can it explain the marked rise in CD seroprevalence that has occurred in the last decades.[6]

The most important environmental factor in CD is gluten, present in wheat, rye and barley. But also other nutritional factors such as short duration of breastfeeding have been implicated in the pathogenesis of CD,[7] although recent studies are contradictory.[8]

There is a paucity of research on non-dietary environmental risk factors in CD. Most[9, 10] but not all[11] studies have found an inverse relationship between smoking and adult-onset CD. In 1987, Kagnoff, et al suggested that Adenovirus may play a role in the pathogenesis of CD,[12] and prior rotavirus infection increases the risk of developing CD [13] but not active infection.[14] A recent German study found that earlier gastrointestinal disease (including both infections and non-infectious disease) increased the risk of later CD,[15] while our group found a non-significantly increased risk of CD in children with an infection at time of gluten introduction.[8] Recently we also showed that elective caesarean section is associated with later CD.[16] One potential explanation for the association with elective caesarean section involves changes in the individuals’ microbiota.

Month of birth could potentially affect both the microbiota and the innate immune system of individuals at risk of CD through different exposures to infectious disease. We know of five studies on birth season and CD,[13, 1720] but only one of these studies included more than 500 patients with CD[18] and all studies were restricted to CD diagnosed in childhood or adolescence.

We hypothesized that summer birth (children would then be weaned in winter time with heavy exposure to viral infections at time of gluten introduction) would be associated with later CD. This was based on the rationale that children born in summer months are more likely to have initial exposure to gluten during winter months, when concurrent viral infection is more probable. Another potential cause of seasonal variations in CD is lack of vitamin D. Low levels of vitamin D predisposes to certain immune-mediated diseases..[21] For example, individuals born in summer months are at increased risk of multiple sclerosis, perhaps due to lower levels of Vitamin D during foetal development (in wintertime).[22]

To test this hypothesis we examined birth season in a nationwide cohort of CD patients and in their age- and gender-matched controls.

METHODS

We linked data on patients with biopsy-verified CD identified through Sweden’s 28 pathology departments. Controls matched for age, gender, calendar period and county were identified from the Swedish national Total Population Register, where we also obtained data on birth date.

Collection of biopsy data

We collected data on small intestinal biopsies (duodenum and jejunum) performed between July 1969 and February 2008 from all Swedish pathology departments. The data collection took place in 2006–2008. We searched computerized registers for data on arrival date of the biopsies, the personal identity number,[23] and morphology according to SnoMed classification codes. Most pathology departments were computerized in the 1990s and hence most biopsy reports originated after 1990. Initially we collected data on both villous atrophy (VA, Marsh 3), but also milder histopathology (Marsh 0–2).[24] In this study, we equated VA with CD. Later patient chart reviews of a random sample of 114 patients with VA found that 108 (95%) had CD,[25] and a manual review by two independent researchers of more than 1500 biopsy reports with VA or Marsh 1–2 found that other diagnoses than CD were very rare in Swedish patients with VA (the most common non-CD diagnosis was IBD and occurred in 0.3% of all patients with VA). [25] We did not require a positive CD serology for a diagnosis of CD, but in the above sample, 88% of patients undergoing patient chart validation and with available data on CD serology at time of biopsy had a positive serology. [25]

Cases and controls

We initially collected data on 351,403 biopsy reports (in 287,586 unique individuals); 29,148 of these had CD. After exclusion of patients where the biopsy could potentially have been obtained in the ileum, patients without matched controls, and patients who could not be matched by the government agency Statistics Sweden, there remained 29,096 patients with CD. Statistics Sweden then identified up to five controls for each individual with CD (n-total: 144,522). Controls were matched on gender, age, calendar year and county.

Seasonality data

The date of birth of all CD patients and matched controls were obtained from the Swedish Total Population Register, and birth season was dichotomized a priori into summer births (March 1st through August 31st) and winter births (September 1st through February 29th). This season cut-off definition is the same as the two other studies that dichotomized birth season. [18, 19]

Statistical analyses

The primary outcome of interest was the proportion of summer births among CD patients as compared to controls. We used conditional logistic regression to compare CD patients to controls, who were matched by age, gender, and calendar period. We also tested whether the association between CD and birth season varied by age of diagnosis of CD (<2, 2–18, ≥18), gender, and calendar period of diagnosis (≤1989, 1990–1999, ≥2000). We used the Breslow-Day test for homogeneity of odds ratios. All p values reported are two sided. All statistical calculations were performed with SAS version 9.2 (Cary, NC).

Ethics

The study was approved by the Research Ethics Review Board, Stockholm, Sweden. Since none of the participants was contacted and individual information was anonymized prior to the analyses, informed consent was not required.

RESULTS

Background

We used the same set of 29,096 individuals with CD and 144,522 controls matched for age, gender and calendar period as in our previous study on mortality in CD.[26] 16% of CD patients were diagnosed <2 years of age (Table I). Most study participants were female (62%), and the majority were diagnosed after year 1990.

Table I
Characteristics of Coeliac Disease Patients and Controls

Main results

54.10% of individuals with CD vs. 52.75% of controls were born in the summer months (Table I). Summer birth was hence associated with a small increased risk of later CD (Odds ratio, OR: 1.06; 95%CI=1.03–1.08; p<0.0001).

Stratifying CD patients according to age at diagnosis (Table II), we found the highest OR in those diagnosed before age 2 years (OR=1.17; 95%CI=1.10–1.26). Summer birth was not associated with a CD diagnosis in later childhood (age 2–18 years: OR=1.02; 95%CI=0.97–1.08), but weakly associated with an increased risk of CD in adulthood (age ≥18years: OR=1.04; 95%CI=1.01–1.07). The difference in age-specific odds ratios was statistically significant (Breslow-Day test for homogeneity: p=0.0017).

Table II
Distribution of Summer (March-August) vs. Winter (September-February) Births in Coeliac Disease Patients and Controls, Stratified by Age of Diagnosis

Stratified analysis by gender and calendar period is shown in Table III. The association between summer birth and risk of CD was similarly present in males (OR 1.08; 95% CI 1.03–1.12) and females (OR 1.04; 95% CI 1.01–1.08). The association between summer birth and CD decreased over time, and in patients diagnosed from 2000 and onwards, the OR was 1.02 (95% CI 0.98–1.06).

Table III
Distribution of summer (March-August) vs. winter (September-February) births in coeliac disease patients and controls, stratified by gender and year of diagnosis

DISCUSSION

This study found a modest increased risk of later CD in individuals born in summer months. More importantly, the upper 95% CI was 1.08, ruling out a large effect of summer birth on the risk of CD. Overall, seasonal variation in food intake and infectious disease exposures are therefore unlikely to be a major cause of CD.

Of the four earlier studies on this topic, three found a positive association between CD and summer birth in subsets of study participants with CD.[13, 18, 19]

While Ivarsson et al found a 1.4-fold increased risk of future CD in children born in the summer and aged <2 years at diagnosis, they did not see an increased risk in children diagnosed with CD at the age of 2–15 years (relative risk=0.96; 0.81–1.1).[18] Interestingly enough, Lewy et al also found a seasonality pattern in girls diagnosed with CD before 24 months of age but not after,[20] and even if there was a seasonality pattern in boys with CD both before and after 24 months of age, the patterns differed.[20]

Data from a longitudinal study in Denver (54 cases with CD) found that 53% of CD cases vs. 48% of controls were born April-September.[13] A study from Massachusetts, published in abstract form, examined 382 children with CD and reported an increased risk of CD in individuals diagnosed <15 years, but not in individuals diagnosed 15–19 years of age.[19] Kokkonen et al examined 226 Finnish children but observed no association between birth season and risk of CD.[17]

The main difference between our and earlier studies lies in the number of patients with CD. Our study involved more than 29,000 patients with CD compared to 3244 patients in the five earlier studies combined.[13, 1720] This allowed for stratified analyses where we were able to detect a positive association between summer birth and CD in all age strata although only for children diagnosed <2 years, and adults diagnosed ≥18 years were these associations statistically significant. Another difference is our use of matched controls. Using conditional logistic regression approach we could examine study participants per stratum (one individual with CD compared to his or her matched controls), and we were hence able to eliminate the effect of gender, age, calendar period and county on our risk estimates.

We used biopsy data to ascertain CD. An earlier validation study has found that 96% of all Swedish adult gastroenterologists and 100% of all paediatricians biopsy patients with CD before diagnosis.[25] Hence our study is likely to have identified a very high proportion of patients with diagnosed CD. However we did not screen the Swedish population for CD and hence we were unable to study the association between birth season and undiagnosed CD.

It is unlikely that our findings are due to chance. Of the six summer months in this study, there was a positive association with CD in five (August being the exception). Our risk estimate was highly statistically significant (OR 1.06; 95% CI 1.03–1.08, p<0.0001). Still, we urge caution when interpreting our data, as the mechanism for this small association is unknown, it did not occur in the last calendar period, and our findings should therefore not influence how parents plan their conception and pregnancy.

The positive association between summer birth and future CD was only seen in children born until 1999. We cannot explain the lack of association in children born thereafter. One potential explanation is that the lack of statistical significance here is a chance finding, just as the opposite, statistically significant associations, may occur when many comparisions are performed. It is also possible that individuals diagnosed after 1999 are phenotypically different from children born earlier, due to the advent of easily available CD screening tools such as endomysium and tissue transglutaminase antibodies. If summer birth is associated with CD primarily in children with classic symptoms, this could also explain why we saw the highest OR in children diagnosed with CD before 2 years of age (when disease onset often consists of malabsorptive symptoms).

In this study we did not have access to data on exposures to infectious disease, age at gluten introduction and breastfeeding duration. However, we speculate that children born in summer are more exposed to viral infections at time of gluten introduction (winter). In our recent paper on 9,408 children with prospective data on nutrition and infectious disease, infection at time of gluten introduction was associated with an increased risk of future CD although the risk estimate failed to attain statistical significance (adjusted OR=1.8, 95%CI=0.9–3.6).[8] Infections (either viral or bacterial) could potentially increase the risk of CD in several ways. They may influence the microbiota, thereby compromising the mucosal barrier function.[27] Infections may also lead to the release of interferon gamma increasing HLA expression. [28] Importantly, some infections seem to influence tissue transglutaminase release,[29] which is a crucial factor in CD.

Another possible mechanism is the effect of low vitamin D levels both in the mother due to lack of UV light exposure in mid-pregnancy or in the child at the time of gluten introduction or viral infection.[22]

In conclusion, summer birth was associated with an increased risk of later CD, but the excess risk was small, largely limited to children diagnosed before age 2, and was not seen in the last calendar period. Seasonal exposures early in life, such as infectious diseases, are unlikely to be a major cause of CD. Future studies should focus on testing putative mechanisms underlying this modest association between birth season and risk of CD.

What is already known on this topic

  • Environmental factors play a role in the aetiology of coeliac disease
  • There is little knowledge about non-nutritional environmental factors in coeliac disease

What this study adds

  • Birth in the summer months was a risk factor for coeliac disease.
  • Summer birth as a risk factor was most prominent for children diagnosed with coeliac disease before age 2.
  • Seasonal differences in coeliac disease risk were small.

Acknowledgments

Funding

BL: The American Scandinavian Foundation, the Coeliac Sprue Association, and the National Center for Research Resources, a component of the National Institutes of Health (KL2 RR024157).

JAM: The National Institutes of Health – DK071003 and DK057892.

JFL: Örebro University Hospital, Karolinska Institutet, the Swedish Society of Medicine, the Swedish Research Council – Medicine (522-2A09-195), the Swedish Coeliac Society and the Fulbright Commission.

Abbreviations used in this article

CD
Coeliac disease
CI
Confidence Interval
HR
Hazard Ratio
VA
Villous atrophy

Footnotes

Details of ethics approval: This project (2006/633-31/4) was approved by the Research Ethics Review Board, Stockholm, Sweden on June 14th, 2006.

Guarantor:

JFL

Competing interests

None

Contributions:

ICMJE criteria for authorship read and met: BL, PG, JM, JFL.

Agree with the manuscript’s results and conclusions: BL, PG, JM, JFL.

Designed the experiments/the study: BL, JFL.

Collected data: JFL.

Analyzed the data: BL.

Wrote the first draft of the paper: JFL.

Contributed to the writing of the paper: BL, PG, JM, JFL.

Contributed to design of study and interpretation of the data analyses: BL, PG, JM, JFL.

Interpretation of data, approved the final version of the manuscript: BL, PG, JM, JFL.

Responsible for data integrity: JFL.

Supervised the project including data analyses: JFL.

Obtained funding: JFL.

References

1. Walker MM, Murray JA, Ronkainen J, et al. Detection of Celiac Disease and Lymphocytic Enteropathy by Parallel Serology and Histopathology in a Population-Based Study. Gastroenterology. 2010;139:112–9. [PMC free article] [PubMed]
2. Karell K, Louka AS, Moodie SJ, et al. HLA types in celiac disease patients not carrying the DQA1*05-DQB1*02 (DQ2) heterodimer: results from the European Genetics Cluster on Celiac Disease. Hum Immunol. 2003;64:469–77. [PubMed]
3. Sollid LM, Qiao SW, Anderson RP, et al. Nomenclature and listing of celiac disease relevant gluten T-cell epitopes restricted by HLA-DQ molecules. Immunogenetics. 2012;64:455–60. [PMC free article] [PubMed]
4. Nistico L, Fagnani C, Coto I, et al. Concordance, disease progression, and heritability of coeliac disease in Italian twins. Gut. 2006;55:803–8. [PMC free article] [PubMed]
5. Sollid LM. Coeliac disease: dissecting a complex inflammatory disorder. Nat Rev Immunol. 2002;2:647–55. [PubMed]
6. Catassi C, Kryszak D, Bhatti B, et al. Natural history of celiac disease autoimmunity in a USA cohort followed since 1974. Ann Med. 2010;42:530–8. [PubMed]
7. Ivarsson A, Hernell O, Stenlund H, et al. Breast-feeding protects against celiac disease. Am J Clin Nutr. 2002;75:914–21. [PubMed]
8. Welander A, Tjernberg AR, Montgomery SM, et al. Infectious disease and risk of later celiac disease in childhood. Pediatrics. 2010;125:e530–6. [PubMed]
9. Snook JA, Dwyer L, Lee-Elliott C, et al. Adult coeliac disease and cigarette smoking [see comments] Gut. 1996;39:60–2. [PMC free article] [PubMed]
10. Suman S, Williams EJ, Thomas PW, et al. Is the risk of adult coeliac disease causally related to cigarette exposure? Eur J Gastroenterol Hepatol. 2003;15:995–1000. [PubMed]
11. Ludvigsson JF, Montgomery SM, Ekbom A. Smoking and celiac disease: a population-based cohort study. Clin Gastroenterol Hepatol. 2005;3:869–74. [PubMed]
12. Kagnoff MF, Paterson YJ, Kumar PJ, et al. Evidence for the role of a human intestinal adenovirus in the pathogenesis of coeliac disease. Gut. 1987;28:995–1001. [PMC free article] [PubMed]
13. Stene LC, Honeyman MC, Hoffenberg EJ, et al. Rotavirus infection frequency and risk of celiac disease autoimmunity in early childhood: a longitudinal study. Am J Gastroenterol. 2006;101:2333–40. [PubMed]
14. Rostami-Nejad M, Rostami K, Sanaei M, et al. Rotavirus and coeliac autoimmunity among adults with non-specific gastrointestinal symptoms. Saudi Med J. 2010;30:891–4. [PubMed]
15. Decker E, Engelmann G, Findeisen A, et al. Cesarean delivery is associated with celiac disease but not inflammatory bowel disease in children. Pediatrics. 2010;125:e1433–40. [PubMed]
16. Marild K, Stephansson O, Montgomery S, et al. Pregnancy outcome and risk of celiac disease in offspring: a nationwide case-control study. Gastroenterology. 2012;142:39–45. e3. [PMC free article] [PubMed]
17. Kokkonen J, Simila S, Vuolukka P. The incidence of coeliac disease and pyloric stenosis in children in Northern Finland. Ann Clin Res. 1982;14:123–8. [PubMed]
18. Ivarsson A, Hernell O, Nystrom L, et al. Children born in the summer have increased risk for coeliac disease. J Epidemiol Community Health. 2003;57:36–9. [PMC free article] [PubMed]
19. Tanpowpong P, Vassallo M, Katz AK, et al. Season of Birth and Celiac Disease in Massachusetts Children. Gastroenterology; Conference on Digestive Disease Week; 2011; Chicago. 2011. pp. S442–S.
20. Lewy H, Meirson H, Laron Z. Seasonality of birth month of children with celiac disease differs from that in the general population and between sexes and is linked to family history and environmental factors. J Pediatr Gastroenterol Nutr. 2009;48:181–5. [PubMed]
21. Munger KL, Levin LI, Hollis BW, et al. Serum 25-hydroxyvitamin D levels and risk of multiple sclerosis. JAMA. 2006;296:2832–8. [PubMed]
22. Salzer J, Svenningsson A, Sundstrom P. Season of birth and multiple sclerosis in Sweden. Acta Neurol Scand. 2010;122:70–3. [PubMed]
23. Ludvigsson JF, Otterblad-Olausson P, Pettersson BU, et al. The Swedish personal identity number: possibilities and pitfalls in healthcare and medical research. Eur J Epidemiol. 2009;24:659–67. [PMC free article] [PubMed]
24. Marsh MN. Gluten, major histocompatibility complex, and the small intestine. A molecular and immunobiologic approach to the spectrum of gluten sensitivity (‘celiac sprue’) Gastroenterology. 1992;102:330–54. [PubMed]
25. Ludvigsson JF, Brandt L, Montgomery SM, et al. Validation study of villous atrophy and small intestinal inflammation in Swedish biopsy registers. BMC Gastroenterol. 2009;9:19. [PMC free article] [PubMed]
26. Ludvigsson JF, Montgomery SM, Ekbom A, et al. Small-intestinal histopathology and mortality risk in celiac disease. JAMA. 2009;302:1171–8. [PubMed]
27. Sanz Y, De Pama G, Laparra M. Unraveling the ties between celiac disease and intestinal microbiota. Int Rev Immunol. 2011;30:207–18. [PubMed]
28. Koning F. Celiac disease: quantity matters. Semin Immunopathol. 2012;34:541–9. [PMC free article] [PubMed]
29. Sarmiento L, Galvan JA, Cabrera-Rode E, et al. Type 1 diabetes associated and tissue transglutaminase autoantibodies in patients without type 1 diabetes and coeliac disease with confirmed viral infections. J Med Virol. 2012;84:1049–53. [PubMed]