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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Braz Oral Res. Author manuscript; available in PMC 2013 January 30.
Published in final edited form as:
PMCID: PMC3558839
NIHMSID: NIHMS374271

Genetics and Caries – Perspectives1

Alexandre Rezende Vieira, DDS, MS, PhD, Associate Professor and Director of Clinical Research

Abstract

Caries remains the most prevalent non-contagious infectious disease in humans. It is clear that the current approaches to decrease the prevalence of caries in human populations, including water fluoridation and school-based programs, are not enough to protect everyone. The scientific community has suggested the need for innovative work in a number of areas in cariology, encompassing disease etiology, epidemiology, definition, prevention, and treatment. We have pioneered the work on genetic studies to identify genes and genetic markers of diagnostic, prognostic, and therapeutic value. This paper summarizes a presentation that elaborated on these initial findings.

Keywords: Dental Caries, Genetics, Genes

Although there is growing evidence that genetics contributes to caries, historically there has been a lack of studies approaching the problem from this front. Caries is the consequence of the interaction of host factors, microbial infection, and substrate that favors the cariogenic microbiota. It is not difficult to propose underlying genetic mechanisms modulating each of these factors, such as saliva factors, which influence bacterial adhesion or acidic buffer capacity.

When looking at models that can incorporate genetic influences, it is important to consider the facts. In regards to types of diet and sugar consumption, a decline in caries experience based on DMFT/dmft scores in the past 5 decades in the US can be seen despite the very high sugar intake of the population1,2. This decline is likely due to measures such as school-base education programs and fluoride exposure and in general promoting dietary changes alone have little or no impact in the future caries experience of the population. Water fluoridation has also shown an effect in caries experience in the US in the same period, with a decline in DMFT scores. However, not all individuals living in fluoridated areas experienced less disease, suggesting fluoride alone is not enough to protect everyone.

Human models, including studies with twins, provide evidence that caries has a genetic component. The classic Vipeholm study3 clearly showed that the increased exposure to foods rich in sugar increased the severity of caries, but even though the individuals in the study consumed caramels four times a day in between meals, 20% of them had not developed any caries lesions after one year. This result suggests individual susceptibility also modulates caries experience. Only one publication related to the Vipeholm study looked at familial aggregation related to caries, and it showed that parents and siblings of subjects of the Vipeholm study who did not develop any caries lesions had significantly lower caries prevalence than the parents and siblings of the remaining subjects4. Twin studies also provide evidence that genetics influence caries. Several variables related to caries experience (i.e. number of teeth present, percentage of teeth restored, percentage of surfaces restored, percentage of teeth affected by caries, percentage of surfaces affected by caries) show statistical significant concordance rates in monozygote twins, but not in dizygote twins5. These studies measure the heritability, or the amount of variation in the disease frequency that is due to genetic variation, and twins have been studied in the US, Brazil, and China, with heritability values ranging from 25% to 80%, depending on the disease-related variable studied.

Animal models have also been used to investigate the genetics contribution to caries, particularly in Japan. The basic design of this approach involves crossing strains particularly susceptible to the disease with strains resistant. Loci in chromosomes 1, 2, 7, 8, and 17 were suggested to contribute to caries susceptibility68.

Our studies of the genetics contribution to caries involve two study designs: candidate gene approaches and genome wide scan approaches. Since caries is particularly influenced by the environment (oral hygiene habits, types of diet, fluoride exposure, access to care), the main emphasis of our group is characterizing the study population to decrease heterogeneity. We observed that a collection of about 100 dry skulls derived from individuals that lived in the northeast of the United States more than 100 years ago had high caries experience, but not all specimens had signs of the disease9. These individuals lived in times prior to organized dentistry and likely had the same oral hygiene practices and dietary habits of the general population. Since we have access to population samples from many areas, we decided to focus on groups with limited access to dental care and information about oral health. We performed the first genome wide linkage studies in caries in a group of families from the Philippines. These families lived in rural areas and had similar cultural and behavioral habits. We detected suggestive linkage between low caries experience and loci 5q13.3, 14q11.2, and Xq27.1. Also, high caries experience was linked to loci 13q31.1 and 14q24.310. The candidate gene approaches include three main groups of genes: genes involved in enamel development, saliva formation and composition, and immune response. These studies include population from Tiquisate - Guatemala, Istanbul - Turley, Pittsburgh - USA, and the Patagonian region of Argentina. All these populations have similar socioeconomic status and access to dental care. Initial results are very encouraging and suggest associations can be found between these candidate genes and high caries susceptibility1113.

CONCLUSION

In summary, we believe genetic susceptibility to caries can be identified under specific experimental conditions. Several genes likely influence individual susceptibility to caries, and these include genes involved in enamel development, saliva function, and immune response.

Footnotes

1Paper presented at the “Oral health Promotion: Expanding the Boundaries of Knowledge” National Symposium, heal at the 16th Congress of the Brazilian Association for Oral Health Promotion (ABOPREV), July 1st 2011, Brasília, DF, Brazil.

The author has no conflict of interest to declare.

Literate Cited

1. Welsh JA, Sharma A, Abramson JL, Vaccarino V, Gillespie C, Vos MB. Caloric sweetener comsumption and dyslipidemia among US adults. J Am Med Assoc. 2010 Apr;303(15):1490–1497. [PMC free article] [PubMed]
2. Brown LJ, Wall TP, Lazar V. Trends in caries among adults 18 to 45 years old. J Am Dent Assoc. 2002 Jul;133(7):827–834. [PubMed]
3. Gustafsson BE, Quensel CE, Lanke LS, Lundqvist C, Grahnén H, Bonow BE, Krasse B. The Vipeholm Dental Caries Study. The effects of different levels of carbohydrate intake in 436 individuals observed for five years. Acta Odontol Scand. 1954 Sep;11(3–4):232–364. [PubMed]
4. Böök JA, Grahnén H. Clinical and genetical studies of dental caries. II. Parents and sibs of adult highly resistant (caries-free) propositi. Odontol Revy. 1953 Jan;4(1):1–53.
5. Boraas JC, Messer LB, Till MJ. A genetic contribution to dental caries, occlusion, and morphology as demonstrated by twins reared apart. J Dent Res. 1988 Sep;67(9):1150–1155. [PubMed]
6. Suzuki N, Kurihara Y, Kurihara Y. Dental caries susceptibility in mice is closely linked to the H-2 region on chromosome 17. Caries Res. 1998 Jul-Aug;32(4):262–265. [PubMed]
7. Uematsu T, Nariyama M, Shimizu K, Maeda T. Mapping of affected gene(s) to dental caries susceptibility on mouse chromosome 2. Pediatr Dent J. 2003 Jan;13(1):75–81.
8. Nariyama M, Shimizu K, Uematsu T, Maeda T. Identification of chromosomes associated with dental caries susceptibility using quantitative trait locus analysis in mice. Caries Res. 2004 Mar-Apr;38(2):79–84. [PubMed]
9. Rose EK, Vieira AR. Caries and periodontal disease: insights from two US populations living a century apart. Oral Health Prev Dent. 2008 Jan;6(1):23–28. [PubMed]
10. Vieira AR, Marazita ML, McHenry TG. Genome-wide scan finds suggestive caries loci. J Dent Res. 2008 May;87(5):435–439. [PubMed]
11. Deeley K, Letra A, Rose EK, Brandon CA, Resick JM, Marazita ML, Vieira AR. Possible association of amelogenin to high caries experience in a Guatemalan-Mayan population. Caries Res. 2008 Jan-Feb;42(1):8–13. [PMC free article] [PubMed]
12. Patir A, Seymen F, Yildirim M, Deeley K, Cooper ME, Marazita ML, Vieira AR. Enamel formation genes are associated with high caries experience in Turkish children. Caries Res. 2008 May-Jun;42(5):394–400. [PMC free article] [PubMed]
13. Ozturk A, Famili P, Vieira AR. The antimicrobial peptide DEFB1 is associated with caries. J Dent Res. 2010 Jun;89(6):631–636. [PubMed]