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The orientation and direction of the pilE G4 sRNA is required for its function.
A. The pilE G4 sRNA region. The C-rich strand (blue) and G-rich strand (pink) are designated. A triangle indicates a transposon insertion used for selection of transformation. B. The pilE G4 sRNA endogenous locus mutants. A representation of the inverted, reverse, and reverse-inverted sRNA mutants is shown. A triangle indicates a transposon used as a marker for transformation. C. Kinetic pilus-dependent colony phase variation assay. The pilE G4 sRNA inverted, reverse, and reverse-inverted do not allow pilin Av. P<0.05 as determined by two-tailed Student's T-test (asterisks). Error bars represent the standard error of the mean for 10 colonies. A representative assay of n=3 is shown.