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Genetic analysis of pilE G4-associated sRNA.
The pilE G4-associated sRNA promoter (boxed), start site (+1) and surrounding sequence are shown. Arrows indicate the location of insertions (+) and point mutations (pt mts) while deletions (−) are underlined in red. Whether these mutations resulted in wild-type levels of pilin Av (Av), an Av deficient (Avd), or an Av intermediate (Avi) phenotype is also indicated. These Av phenotypes were determined by a pilus-dependent colony phase variation kinetic assay  which is shown in Fig. S1.