Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts

doi:10.1038/nature09637

Nature. Author manuscript; available in PMC 2013 January 17.

Published in final edited form as:

Nature. 2011 January 20; 469(7330): 415–418.

Published online 2010 November 28. doi: 10.1038/nature09637

PMCID: PMC3547360

NIHMSID: NIHMS301786

Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts

Toshiro Sato, Johan H. van Es, Hugo J. Snippert, Daniel E. Stange, Robert G. Vries, Maaike van den Born, Nick Barker, Noah F. Shroyer,¹ Marc van de Wetering, and Hans Clevers

Hubrecht Institute, Uppsalalaan 8, 3584CT Utrecht, the Netherlands

¹Cincinnati Children’s Hosp, Div Gastroenterology, Med Ctr, MLC 2010,3333 Burnet Ave, Cincinnati, OH 45229 USA

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Abstract

Homeostasis of self-renewing small intestinal crypts results from neutral competition between Lgr5 stem cells, small cycling cells located at crypt bottoms^{1, 2}. Lgr5 stem cells are interspersed between terminally differentiated Paneth cells, that are known to produce bactericidal products such as lysozyme and cryptdins/defensins³. Single Lgr5-expressing stem cells can be cultured to form long-lived, self-organizing crypt-villus organoids in the absence of non-epithelial niche cells⁴. Here, we note a close physical association of Lgr5 stem cells with Paneth cells in vivo and in vitro. CD24⁺ Paneth cells express EGF, TGFα, Wnt3 and the Notch-ligand Dll4, all essential signals for stem cell maintenance in culture. Co-culturing of sorted stem cells with Paneth cells dramatically improves organoid formation. This Paneth cell requirement can be substituted by a pulse of exogenous Wnt. Genetic removal of Paneth cells in vivo results in the concomitant loss of Lgr5 stem cells. In colon crypts, CD24⁺ cells residing between Lgr5 stem cells may represent the Paneth cell equivalents. We conclude that Lgr5 stem cells compete for essential niche signals provided by a specialized daughter cell, the Paneth cell.

In a Matrigel-based culture system containing EGF, the Wnt agonist Rspondin1 and the BMP inhibitor Noggin⁴, single Lgr5 stem cells autonomously grow into crypt-like structures with de novo generated stem cells and Paneth cells at their bottom. The remainder of these crypts consists of transit-amplifying cells, which feed into villus-like luminal domains containing post-mitotic enterocytes and goblet cells. Thus, a single Lgr5 intestinal stem cell can generate a continuously expanding, self-organizing organoids reminiscent of normal gut in the absence of a subepithelial cellular niche. Confocal cross-sectioning of crypt bottoms of Lgr5-EGFP-ires-CreERT2 mice revealed an almost geometrical distribution of Paneth cells and Lgr5 stem cells, that maximized heterotypic contact area (Paneth-stem cell) and minimized homotypic contact area (Fig 1a-c). The same intimate contact was observed in the organoid cultures at crypt bottoms (Fig 1d and Suppl Movie 1).

Figure 1

Geometric distribution pattern of Paneth cells and Lgr5 stem cells

The hypothesis that Paneth cells supply essential niche signals was rejected previously⁵. To retest this, stem cells sorted from Lgr5-EGFP-ires-CreERT2 mice based on GFP expression⁶, were recombined with wild type Paneth cells sorted for CD24 expression (Fig 2a/c). Of note, CD24-expressing cells reside between Lgr5 stem cells in colon crypts (Fig 2b), suggesting that these are related to Paneth cells. Indeed, a secretory cell type –distinct from goblet cells-resides at colon crypt bottoms⁷. Stem cells and/or Paneth cells were seeded in round-bottom plates in 10% Matrigel. Reassociated Lgr5 stem cells typically formed short-lived, cystic clusters (Fig 2d). Reassociated Paneth cells tended to form larger aggregates (Fig 2e), which disintegrated after 5 days. In three independent experiments, long-lived GFP organoids were formed in only 6.7±3.3% of 10 wells per experiment containing 500 Lgr5 stem cells each, and in 0% of 10 wells per experiment containing 500 Paneth cells each (we occasionally observed GFP-negative organoids originating from contaminating wild type Paneth cells). When 500 stem cells and 500 Paneth cells were combined, GFP⁺ organoids formed in 76.7±8.8% of 10 wells per experiment (Fig 2f/g). The dynamic reassociation process was illustrated using Lgr5⁺ cells sorted from a clonal RFP⁺ organoid culture and Paneth cells from a clonal YFP⁺ organoid culture. As shown in a movie (Fig 2h and Suppl Movie 2), multiple cell clusters formed initially. The organoids fused to form one or two large organoids per well (Suppl Movie 2). Thus, Lgr5 stem cells and Paneth cells appeared to require physical contact. Indeed, Lgr5 stem cells are critically dependent on Notch signals ^8-10, which depend on direct cell-cell contact.

Figure 2

Paneth cells express CD24 and support growth of Lgr5 stem cells

We then performed comparative gene expression profiling on stem and Paneth cells. The heat map in Fig 3a confirmed the segregation of Paneth cells markers (lysozyme, DefensinA1³) and stem cell markers (Lgr5, Olfm4, Tnfrsf19, CDCA7¹¹). Amongst the genes most highly enriched in Paneth cells, we noted Wnt3 and Wnt11, EGF and TGFα, and the Notch ligand, Dll4 (Fig 3a). Wnt3 expression¹² and EGF expression¹³ had been noted previously. Thus, Paneth cells provided essential signals for stem cell support: EGF, Wnt3 and Notch. High level expression of Wnt3 was confirmed by in situ hybridization (Fig 3b).

Figure 3

Paneth cells produce Wnt3 and other essential niche signals for Lgr5 stem cells

Rspondin1 potently amplifies Wnt responses, yet is inactive on its own¹⁴. When organoids were grown from crypts derived from the Axin2-LacZ mice¹⁵, Wnt responses as assayed by lacZ expression were restricted to the crypt base, despite the ubiquitous presence of Rspondin1 (Fig 3c/e, Suppl Fig 1). When exogenous Wnt3A was added, the organoids diffusely expressed the blue Wnt reporter (Fig 4g). The global response to Wnt caused the typical crypt-villus architecture to change into rounded cysts devoid of differentiated cell types (Fig 3e, g). Indeed, Wnt signaling instructs intestinal cells to adopt a proliferative progenitor phenotype¹⁶. The same rounded cysts were routinely observed upon culturing Apc deficient cells from APC^min adenomas¹⁷ (Fig 3d). When the small molecule Wnt secretion inhibitor (Porcupine inhibitor) IWP1¹⁸ was added, the Axin2-LacZ signal in wt organoids was entirely lost and proliferation halted (Compare Fig 3e and f; a dose-response curve given in Suppl Fig 2). This inhibition could be overcome by exogenous Wnt3A (Fig 3g/h; Suppl Fig 2), confirming the specificity of the Wnt secretion inhibitor. We concluded that exogenous Rspondin1 acts by amplifying the local response to short-range Wnt produced by Paneth cells. Thus, only the direct neighbors of Paneth cells, the Lgr5 stem cells, receive strong Wnt signals, which can be further increased by Rspondin1. Moreover, these observations implied that the asymmetry of crypt-villus organoids was established by the localized presence of Wnt-producing Paneth cells. We recently observed that stem cell-Paneth cell doublets display a strongly increased plating efficiency compared to single stem cells². This Paneth cell-dependence of single stem cells, illustrated in suppl Fig3, could be overcome by addition of Wnt-3A at 100 ng/ml for the first three days of culture (Fig 3i).

Figure 4

Paneth cells regulate numbers of intestinal stem cells in vivo

To investigate in in vivo models whether Paneth cells provide essential support to Lgr5 stem cells, we utilized three previously described genetic mouse models for Paneth cell loss: mutation of Gfi1¹⁹, transgenic expression of Diphtheria Toxin A under the Paneth cell-specific Cryptdin2 promoter (CR2-tox176⁵) and conditional deletion of Sox9^20,21. When we re-visited crypts of Gfi1-/- adult mice, described to lack Paneth cells ¹⁹, Paneth cell numbers were reduced but not absent, as also seen recently ²². Lysozyme staining revealed that the majority of crypts harbored at least one Paneth cell (Fig 4a-b; Suppl Fig 4; Sequential section analysis revealed that >90% of crypts possess more than one Paneth cells (not shown)). Stem cells were coincidently decreased in number (Fig 4d-e) and colocalized with remaining Paneth cells as visualized by double staining for Olfm4 and Lysozyme (Fig 4g-h). Similarly, in the CR2-tox176 mice, we noted that Paneth cells were reduced but present (Fig 4c; Suppl Fig 4) in agreement with the reported 82% decrease in Paneth cell numbers ⁵. Numbers of stem cells were again decreased, coincident with Paneth cells (Fig4c, f, i-k).

We conditionally deleted the Sox9 gene in 6-week old mice, homozygous for a Sox9^flox allele and heterozygous for the Ah-Cre allele²³. Paneth cells are estimated to have a life-time of 8 weeks²⁴. The Sox9 gene was efficiently deleted in all crypt cells with the exception of pre-existing Paneth cells, where the Ah-Cre transgene is not activated ²³. Although Sox9 is expressed in Lgr5 stem cells ¹¹, we observed no stem cell phenotype at early time points post-deletion (Suppl Fig 5a/b). From 4 weeks onwards, Paneth cell numbers visibly decreased. Loss was virtually complete after 7-8 weeks (Fig 4s), after which a regenerative response occurred. We occasionally noted Sox9-/- crypts with a single remaining Sox9-positive Paneth cell (Suppl Fig 5c; red arrows). Stem cells disappeared coincident with Paneth cells, and remaining stem cells crowded around remaining Paneth cells (Suppl Fig 5d). Suppl. Fig 6 depicts a field of escaper wt crypts adjacent to a field of Sox9-negative crypts. The escaping wt crypts containing abundant Paneth cells rapidly replaced the mutant crypts by crypt fission (Suppl Fig 6). By day 67, all crypt basal cells were Sox9+ again (Fig 4n) and contained normal numbers of Paneth cells (Fig 4t) and stem cells (Fig 4q). From this, we concluded that Paneth cells are essential for maintenance of crypts and stem cells.

Stem cell niches are typically portrayed as pre-existing sites, to which stem cells migrate ²⁵. Here we show that intestinal stem cells receive niche support from their own specialized progeny. This is not without precedent, as the somatic stem cells of the fly testis give rise to differentiated cells that in turn build the testis niche ²⁶. Thus, Paneth cells serve as multifunctional guardians of stem cells, both by secreting bactericidal products and by providing essential niche signals. Lgr5 stem cells divide symmetrically and their numbers are restricted by neutral competition at the stem cell population level². We now propose that the Lgr5 stem cells compete for available Paneth cell surface. Paneth cell numbers must therefore be tightly regulated, which is indeed the case. Paneth cells are born directly above the crypt base, the latter originally termed the “stem cell zone” ^27,28. It will be of interest to understand what determines Paneth cell numbers and their slow turnover rate.

Methods (On line version)

Reagents

Murine recombinant EGF and Noggin (Peprotech). Murine recombinant Wnt-3a (Millipore). Human recombinant R-spondin 1 provided by Arie Abo ²⁹. Y-27632 (Sigma). IWP1 provided by Lawrence Lum ¹⁸.

Mice

Lgr5-EGFP-Ires-CreERT2 mice ¹, APC^min¹⁷, Axin2-lacZ mice ¹⁵, Gfi1-/-¹⁹, CR2-tox17⁵, Sox9^fl/fl²⁰ and R26R-confetti ² mice were described earlier. The transgenic Ah-Cre line ²³ was crossed with Sox9^fl/fl mice. Cre enzyme was induced by intraperitoneal injections of 200 μl β-napthoflavone (10 mg ml^-1; Sigma Aldrich) dissolved in corn oil for three consecutive days.

Crypt isolation, cell dissociation and culture

Described previously ^{2, 4}. For culture/sorting experiments, at least 3 independent experiments were performed. For each experiment, crypts/cells were pooled from 3 intestines. For microarray, sorted cells from 10 intestines were pooled. Crypts were directly cultured as previously described (100 crypts/well on 24-well plate) ⁴. For single-cell or doublet-cell culture, crypts were dissociated with TrypLE express (Invitrogen) including 2000 U/ml DNase (Sigma) for 30 min at 37 °C or 2 hr at room temperature. For reassociation assay from established crypt organoids, the samples were dissociated with TrypLE express for 15 min at 37 °C. Dissociated cells were passed through 20- μm cell strainer (Celltrix) and washed with PBS. Cells were stained with PE- conjugated anti-CD24 antibody (eBioscience) and APC- conjugated anti-Epcam antibody (eBioscience) for 15 min at 4 °C, and analyzed by MoFlo (DakoCytomation). Viable epithelial single-cells or doublets were gated by forward scatter, side scatter and pulse-width parameter, and negative staining for propidium iodide or 7-ADD (eBioscience). Sorted cells were collected, pelleted, and embedded in Matrigel (BD bioscience), followed by seeding on 96-well plate (30-50 singlets or doublets/10 μl Matrigel/well. Culture medium (Advanced DMEM/F12 supplemented with Penicillin/Streptomycin, 10 mM Hepes, Glutamax, 1× N2, 1× B27 (all from Invitrogen), and 1 μM N-acetylcysteine (Sigma) containing growth factors: 50 ng/ml EGF, 100 ng/ml noggin, 1 μg/ml R-spondin) were overlaid. Y-27632 (10 μM) was included for the first 2 days to avoid anoikis. Growth factors were added every other day and the entire medium was changed every 4 days. In some experiments, 100 ng/ml Wnt-3a (Millipore) was added in the culture medium. Sorted cells were manually inspected by inverted microscopy, and the numbers of viable organoids in triplicate were calculated.

Reassociation assay

500 Sorted Lgr5-GFP^hi stem cells (purity >99%) were cocultured with 500 genetically unmarked CD24⁺ Paneth cells (purity >95%). Cells were resuspended in 100 μl of culture medium in Ultra-low attachment 96 well round-bottom plates (Corning) and the plate was left on ice for 15 min. The plate was then centrifuged (300g) for 5 min and 10 μl of Matrigel was added in each well. For Suppl Movie 2, Lgr5-GFP^hi/Confetti-RFP⁺ and Lgr5-GFP^hi/Confetti-YFP⁺ stem cells were sorted separately from Lgr5-EGFP-Ires-CreERT2 × R26R-confetti mice ², tamoxifen-induced three days prior to sacrifice of the mice. After ten days of culture, 1500 Lgr5-GFP^hi/ CD24^dim/Confetti-RFP⁺ stem cells and 1500 CD24^hi/Confetti-YFP⁺ Paneth cells were sorted from these two respective organoid cultures, and filmed for ten consecutive days, interrupted twice for exchange of medium. The fluorescent and phase contrast images were acquired every 20 min by inverted microscopy (AF7000, Leica) equipped with live imaging chamber (humidified with sterile water and maintained at 37 °C, 7.5% CO₂).

Microarray analysis

Single Lgr5-GFP^hi cells or Paneth cells were sorted into Buffer RLT in RNeasy Micro Kit (Qiagen). Microarray analysis (Agilent) was performed as previously described ⁴. The data will be deposited to the GEO database. Heatmaps were created using Treeview software.

Histology, immunohistochemistry and in situ hybridization

Samples taken from the middle of the small intestine were fixed in 4% paraformaldehyde (PFA), embedded in paraffin, and processed as previously described1. The primary antibodies were; mouse anti-E-cadherin (1:100, BD transduction), mouse anti-Ki67 (1:250, Monosan), mouse anti-phospho Histone H3 (1:1000, Millipore), rabbit anti-Sox9 (1:600, Millipore), rabbit anti-Lysozyme (1:1000, Dako) and anti-Chromogranin A (1:100, Santa Cruz). The secondary antibodies were peroxidase-conjugated antibodies or Alexa 568-conjugated antibodies. For whole-mount immunostaining, freshly isolated crypts were fixed with 4% PFA, and stained with anti-CD24 antibody (eBioscience) over night at 4 °C. After washing, the samples were incubated with Alexa 568-conjugated anti-Rat antibody over night at 4 °C. DNA was stained by DAPI (Molecular Probe). For counting the number of Lgr5 stem cells (GFP) and Paneth cells (lysozyme), 1 cm² of PFA fixed intestinal wall was put in a mold. Four percent low melting point agarose (40°C) was added and allowed to cool on ice. Once solid, a vibrating microtome (HM650, Microm) was used to make semi-thick sections (150 μm) (velocity: 1 mm/s, frequency: 65 Hz, amplitude: 0.9 mm) ². Sections were permeabilized and stained as previously described⁴. Images were acquired with confocal microscopy (Leica, SP5). For in situ hybridization, cRNA probes were generated from full-length cDNA expression vectors (IMAGE consortium or RPZD) with in vitro transcription. The protocol was described elsewhere¹². X-gal staining was performed as previously described⁴.

Supplementary Material

Click here to view.^{(514K, pdf)}

Acknowledgments

We thank H. Begthel, J. Korving and S. van den Brink for technical assistance, and J. Gordon for providing small intestinal sections from Cr2-tox177 mice, Lawrence Lum for providing IWP1 and Arie Abo for R-spondin1.

Footnotes

Declaration of competing financial interests

H.C. is an inventor on several patents involving the culture system in this paper, as is T.S.

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