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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nat Chem. Author manuscript; available in PMC Jun 1, 2013.
Published in final edited form as:
Nat Chem. Dec 2012; 4(12): 996–1003.
doi:  10.1038/nchem.1495
PMCID: PMC3545056
NIHMSID: NIHMS412255
Electronic Tuning of Site-Selectivity
Brandon C. Wilcock, Brice E. Uno, Gretchen L. Bromann, Matthew J. Clark, Thomas M. Anderson, and Martin D. Burke
Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
Site-selective functionalizations of complex small molecules can generate targeted derivatives with exceptional step-efficiency, but general strategies for maximizing selectivity in this context are rare. Here we report that site-selectivity can be tuned by simply modifying the electronic nature of the reagents. A Hammett analysis is consistent with linking of this phenomenon to the Hammond postulate: electronic tuning to a more product-like transition state amplifies site-discriminating interactions between a reagent and its substrate. This strategy transformed a minimally site-selective acylation reaction into a highly selective and thus preparatively useful one. Electronic tuning of both an acylpyridinium donor and its carboxylate counterion further promoted site-divergent functionalizations. With these advances, a range of modifications to just one of the many hydroxyl groups appended to the ion channel-forming natural product amphotericin B was achieved. Thus, electronic tuning of reagents represents an effective strategy for discovering and optimizing site-selective functionalization reactions.
Structurally complex small molecules have an extraordinary capacity to perform a wide range of useful functions. Accessing structural derivatives of these compounds, however, represents a major bottleneck in efforts to understand and/or optimally harness this capacity. Site-selective functionalization represents a frontier synthesis strategy with outstanding potential for addressing this limitation.17 Site-selective acylation has emerged as a particularly promising approach for accessing derivatives of polyhydroxylated natural products, with the capacity for exceptional step-efficiency relative to total synthesis.1,818 Current strategies for achieving selectivity in this context include modifying the steric and/or stereochemical features of the acylating reagents,1,816 or utilizing lipase enzymes.17,18 However, suboptimal site-selectivities too often limit the preparative utility of this approach. New strategies for maximizing site-selectivity or enabling the development of reagents that can override substrate bias to achieve site-divergent functionalizations stand to address these important limitations and thereby have a major impact on small molecule science.
In order for site-selective reactions to occur under kinetic control, information encoded in distinct local chemical environments within a substrate must be effectively translated into substantial differences in the energies of the corresponding transition states for functionalization. The Hammond postulate predicts that as a reaction becomes less exothermic, the corresponding transition state will become more product-like.19 As a consequence, any potentially site-discriminating interactions between reagents and substrates should be amplified, thus leading to enhanced site-selectivities. Although the Hammond postulate has classically been invoked to explain a variety of different trends in reactivity,20 a general approach for engaging this phenomenon to maximize site-selective functionalizations of complex small molecules has not, to the best of our knowledge, been previously reported.
We found inspiration for this approach in the context of asymmetric catalysis, where electronic tuning of reagents can have a substantial impact.2124 For example, with the Jacobsen epoxidation, i.e., more electron-releasing salen ligands lead to substantially increased enantioselectivities.21 Mechanistic studies of this phenomenon are consistent with the Hammond postulate, i.e., electron-releasing ligands create milder manganese-based oxidants which, in turn, react via more product-like transition states.22 This presumably amplifies the enantiotopic face-discriminating interactions between the chiral oxidant and the prochiral olefin thus yielding higher levels of enantioselectivity.22 Guided by the logic outlined above and this encouraging precedent, we hypothesized that increasing the electron-richness of an acyl donor would lead to an increase in the product-like nature of the transition state for rate-limiting acyl transfer,2527 resulting in amplified site-discriminating interactions between an acyl donor and a polyol substrate and thus greater site-selectivity (Fig. 1a).
Figure 1
Figure 1
Overview of an approach to functionalize AmB to probe a possible interaction with ergosterol
The decahydroxylated natural product amphotericin B (AmB) (Fig. 1b) represents an outstanding platform for testing this hypothesis, and recent advances have made the hydroxyl group at C2' a particularly important target for site-selective acylation (Fig. 1b).2835 Specifically, in contrast to the widely accepted channel model,3138 AmB primarily kills yeast via simply binding ergosterol,30 a lipid that is vital for many aspects of yeast physiology. Competitive binding of cholesterol in human cells likely plays an important role in the substantial toxicity of this clinically vital antifungal agent.29 Sterol binding is also critical for formation of the AmB-based ion channel,29 a prototype for the development of small molecules that might replicate the function of deficient protein ion channels that underlie human diseases. For all of these reasons, gaining an atomistic understanding of AmB-sterol interactions represents a critical goal.
The mycosamine appendage is required for binding both ergosterol and cholesterol,29 but the specific role(s) played by the three functional groups at C2', C3', and C4' remain unclear. In a leading model,3135 the C2'-OH is predicted to form a hydrogen bond with the 3β-OH on the A-ring of the sterol (Fig. 1b). If C2'-selective acylation could be achieved, orthogonal protection of the remaining hydroxyl groups and subsequent cleavage of the C2' ester would generate a uniquely exposed hydroxyl group at this position. This site could then be selectively manipulated in a variety of ways to generate derivatives designed to more deeply probe the fundamental underpinnings of AmB function (Fig. 1c).
Separating constitutional isomers of highly complex small molecules can be very challenging. This classic problem is magnified substantially in the case of AmB because the physical properties of this natural product, including its amphipathicity and poor solubility in many organic solvents, make the utilization of standard chromatographic techniques ineffective in many cases. Because of this, and the fact that multiple grams of mono-acylated material would be needed to prepare site-selectively modified derivatives of AmB via the sequence outlined in Fig. 1c, a highly site-selective acylation of the C2' hydroxyl group was required.
As described below, electronic tuning proved to be an exceptionally effective strategy for solving this challenging problem. This methodology enables the rational transformation of a minimally site-selective reaction into a highly selective one that is preparatively useful. Importantly, this approach can be applied to increase site-selectivities even if the factors underlying site-discrimination in a specific case are unknown. We further report the surprising discovery that concomitant electronic tuning of both the acylpyridinium ion and its counterion can lead to site-divergent functionalization reactions. Collectively, these findings establish electronic tuning as a strategy for discovering and optimizing site-selective functionalizations of complex small molecules.
To enable our search for a C2'-selective acylation of AmB, we first protected the C3' amine and C41 carboxylic acid as the corresponding phenylacyl amide30 and methyl ester.39 With the goal of biasing acylation towards the C2' position, we then selectively masked the hydroxyl groups at C13, C3/C5, and C9/C11 as a methyl ketal28,40 and p-methoxybenzylidene acetals, respectively30,41 (see Supplementary Information). Collectively these manipulations provided scalable access to derivative 1, possessing five unprotected secondary hydroxyl groups. Of these remaining sites, steric considerations suggested that the C2'-hydroxyl group might be the most accessible to an acylating reagent.
When 1 was exposed to a standard set of acylating conditions [4-dimethylaminopyridine (DMAP), one equivalent of acetic anhydride (2a), diisopropylethylamine (DIPEA) in THF], a complex mixture containing many different mono-, bis-, and tris-acylated products was observed by HPLC (Table 1, entry 1). Analysis of this product mixture revealed that it contained only 2% of the C2' monoacylated product 3a.
Table thumbnail
Table 1
We thus attempted to achieve site-selective acylation at C2' via screening a large collection of commercial lipase enzymes under a wide range of reaction conditions and using many different acyl donors. However, while some encouraging results were obtained, these enzymatic reactions suffered from low conversions, lack of scalability, and/or poor reproducibility.
An established strategy for enhancing site-discrimination is to increase the steric bulk of the acylating reagent,1,42,43 thereby increasing its sensitivity towards subtle differences in the local steric environments of different alcohols appended to the substrate. Following this approach, we evaluated a series of anhydrides with increasing steric bulk. However, little improvement in site-selectivity was observed with propionic (Supplementary Information) or isobutyric anhydride (2b) (Table 1, entry 2), and no conversion was observed for pivalic anhydride (Supplementary Information). Thus, steric modifications of the anhydride donors were unable to improve site-selectivity with this substrate.
Intrigued by reports that changing the counterion of acylpyridinium complexes can also impact site-selectivity,16,44 we next surveyed the analogous series of sterically modified acyl chlorides. Treatment of 1 with acetyl chloride (2c) again provided a complex mixture of acylated products (Table 1, entry 3), further demonstrating that the site-discriminating features of 1 were subtle, and if there was an inherent preference for reactivity at C2', then this preference was small. Pivaloyl chloride led to no reactivity. Encouragingly, however, when we increased the steric bulk of the acyl chloride donor in the form of isobutyryl chloride 2d, we observed the somewhat selective formation of the major product 3b (Table 1, entry 4, 48% site-selectivity). Although 3b could not be separated from this complex mixture of products via standard silica gel chromatography, carefully optimized preparative HPLC provided a few milligrams of purified material. Characterization by multidimensional 1H NMR analysis and high resolution mass spectrometry established that 3b was monoacylated at C2' (Supplementary Information).
Albeit an important step forward, we were unable to develop a practical process for purifying intermediate 3b on larger scale. Thus, it was ultimately not possible to transform this moderately site-selective acylation into a preparatively useful process. Faced with the need to substantially improve this site-selectivity, we considered the hypothesis that electronic tuning of the acyl donor might have an impact. As shown in Fig. 1a, the Hammond postulate predicts that increasing the electron–richness of the acyl donor will increase the product-like nature of the transition state of rate-limiting acyl transfer. As a result, the site-discriminating interactions between the acyl donor and the polyol substrate should be magnified. This, in turn, should lead to larger differences in the activation energies for acylations of different hydroxyl groups and thus greater site-selectivity.
To test this hypothesis, we alternatively employed electronically tunable para-substituted benzoyl chlorides as acyl donors under otherwise identical reaction conditions. The electron-deficient p-nitrobenzoyl chloride (2e) provided only a modest 39% site-selectivity for formation of the corresponding C2' acylated product 3c (Table 1, entry 5). In contrast, simply switching to the much more electron-rich p-N,N-dimethylaminobenzoyl chloride (2f) donor provided the desired C2' acylated product 3d with an outstanding site-selectivity of 72% (Table 1, entry 6).
To systematically evaluate whether this effect is primarily attributable to the electronic nature of the acyl donor, we performed a Hammett study45 with a series of sterically similar para-substituted benzoyl chlorides. Importantly, control experiments confirmed that for all of these donors acyl transfer was irreversible and the rate of background acylation in the absence of DMAP was negligible (Supplementary Information). Thus, the ratio of site-isomers (C2'/other) is attributable to kinetic selectivity for acyl transfer from the corresponding acylpyridinium complexes to one hydroxyl group versus the others. In turn, with a lack of correction for the minor contributions of multiple acylations noted, this ratio of site isomers is a function of the difference in energies of the transition states of the corresponding acylation reactions (ΔΔG).
As predicted by the analysis in Fig. 1a, C2' site-selectivity progressively decreased as the electron-withdrawing capacity of the para-substituent increased (Fig. 2a). A Hammett plot of log[ratio of site-isomers (C2'/other)] vs. σpara revealed a linear correlation with a negative slope (rho = −0.395) (Fig. 2b). A complementary prediction of the analysis presented in Fig. 1a is that the reaction rate should also exhibit a linear correlation with σpara, but in the opposite direction. Specifically, as the electron-withdrawing character of the substituent on the aryl ring increases, the reaction rate should also increase. We tested this prediction directly by determining the relative initial rates for the same five reactions and in fact observed a linear and positive correlation between log(initial rate) and σpara (Fig. 2c). Combining these experiments, a plot of C2' selectivity vs. initial rate (Fig. 2d) also revealed a linear correlation, as collectively predicted by the Hammond analysis presented in Fig. 1a. These results demonstrate that electronic tuning of reagents can have a substantial impact on site-selective functionalization of a complex small molecule substrate, and that this effect is consistent with the Hammond postulate.
Figure 2
Figure 2
Analysis of acylation reactions with para-substituted benzoyl chlorides
With this concept established, an interesting observation from our earlier studies caused us to further question whether electronic tuning might also contribute to another frontier challenge in the area of site-selectivity, i.e., the development of reagent based site-divergent functionalization reactions. Specifically, although neither reaction was highly site-selective, we noted that acylations with isobutyryl chloride 2d (Table 1, entry 4) and the corresponding anhydride 2b (Table 1, entry 2) produced different outcomes.16,44 As described above, the major product derived from 2d is mono-acylated at C2'. In contrast, HPLC purification and multidimensional NMR characterization of the mixture of products formed from anhydride 2b revealed a nearly stoichiometric mixture of derivatives mono-acylated at C4' and C15 (Supplementary Information).
We recognized that electronically modified derivatives of benzoic anhydrides would translate into concomitant electronic tuning of both the acylpyridinium ion intermediate and its associated carboxylate counterion. Because both of these components are thought to play a role during rate-limiting acyl transfer,2527 we anticipated that electronic tuning of these anhydrides might also have a substantial impact on site-selectivity. Due to the combinatorial and potentially competing nature of the effects, however, the specific outcome was difficult to predict in this case. When we reacted 1 with either the electron-rich p-tertbutylbenzoic anhydride 2gpara t-Bu = −0.20) or its electron-deficient counterpart p-nitrobenzoic anhydride 2h, a very interesting pair of stereodivergent acylation reactions were observed. Specifically, with the electron-rich anhydride 2g site-selective acylation of the C4' hydroxyl group is the primary pathway yielding 4a as the major product (Table 2, entry 1). In contrast, utilization of the electron-poor anhydride 2h caused a remarkable turnover in site-selectivity, with a new major product 5b resulting from selective acylation at C15 (Table 2, entry 2). It has been demonstrated that chiral catalysts can be used to achieve reagent-based site-divergent functionalizations of complex small molecules.1,6,811 Importantly, we note that all of the site-divergent functionalizations shown in this work were achieved using only achiral reagents. Thus, electronic tuning has the potential to provide a highly complementary alternative approach for reagent-based site-divergent functionalizations of complex small molecule substrates.
Table thumbnail
TAble 2
Having established electronic tuning as a strategy for the development of site-selective functionalization reactions, we returned to the initial goal of selectively modifying the C2' position of AmB. A survey of various electron-rich benzoyl donors revealed that p-tertbutylbenzoyl chloride provided an optimized combination of C2' site-selectivity (66%), conversion (68%), and ease of purification of the monoacylated product 3e by standard silica gel chromatography. Importantly, both this reaction and chromatographic purification proved to be readily scalable, providing more than 3 grams of purified 3e (45% isolated yield) from a single run. Thus, electronic tuning can transform a minimally site-selective reaction into a highly selective and preparatively useful process.
With efficient and scalable access to monoacylated derivative 3e in hand, unique exposure and subsequent functionalization of the C2' hydroxyl was readily achieved according to the plan outlined in Fig. 1c. Specifically, as shown in Fig. 3, concomitant protection of the four remaining hydroxyl groups as the corresponding diethylisopropylsilyl (DEIPS) ethers was followed by facile cleavage of the aryl ester at C2' with KCN in MeOH to yield 6. As demonstrated with this transformation, another important benefit of the electronic tuning approach is that it allows relatively mild conditions to be employed to achieve deacylation. This stands in contrast to the much more forcing conditions typically required to remove sterically encumbered acyl groups, which can lead to competitive decomposition of complex small molecule substrates.
Figure 3
Figure 3
Selective functionalizations at the C2' position of AmB
Compound 6, having a uniquely exposed hydroxyl group at C2', has proven to be a highly versatile intermediate. For example, despite the presence of the very sensitive polyene macrolide core, efficient deoxygenation at C2' to form 7 was achieved via nucleophilic displacement of the axial C2'-hydroxyl group to generate the equatorial iodide46 followed by a novel AgOAc-mediated reductive deiodination with NaBH4.47 Alternatively, epimerization at C2'34 to yield 8 was readily achieved using standard Mitsunobu conditions. This approach also provides unique access to AmB-small molecule conjugates48 linked via the C2' hydroxyl group. For example, a molecule of ergosterol was tethered to 6 via simple esterification with acid chloride intermediate 9 to form the novel heterodimer 10, which is reminiscent of the predicted structure of the AmB-ergosterol complex (Fig. 1b).
We have thus far only demonstrated the electronic tuning strategy with AmB, and the generality of this approach remains to be determined.49 However, because our studies are consistent with linking of this effect to the Hammond postulate, and the Hammond postulate is broadly applicable to many reactions, our results suggest that electronic tuning may represent a general strategy for improving site-selective functionalizations of complex small molecules. For example, it may be possible to combine this approach with a range of other nucleophilic catalysts1,815 en route to highly optimized site-selective acylations and/or other group transfer processes.50,51 Moreover, this same approach should be applicable to a wide range of other substrate-based site-selective transformations17 as long as the corresponding functionalization reagents can be tuned electronically. In addition, because increasing the product-like nature of a transition state should increase the consequences of site-discriminating interactions regardless of their origins, this strategy should be applicable even in cases where the specific site-discriminating interactions are not understood. Of course, if the specific underpinnings can be elucidated, then a combination of electronic tuning and rational design of the reagent would potentially be synergistic.
Electronic tuning might also prove to be generally useful in the development of reagent-based site-divergent functionalization reactions. For example, if two different acylation catalysts produce modest levels of site-divergency with the same acid chloride, electronic tuning of the acid chloride should lead to parallel optimization of both site-selectivities, thereby yielding highly optimized site-divergency. Moreover, we found that site divergency can also be achieved via concomitant electronic tuning of both an acylpyridinium ion and its carboxylate counterion, which is accessible by simply modifying the corresponding readily available anhydride donors.
It is also highly notable that all of the site-divergent acylation reactions described herein involve only the use of achiral acylating reagents and catalysts. These findings suggest that physical and/or mechanistic features independent of stereochemistry stand ready to impact site-divergent functionalizations of complex small molecules in ways that exceed the current level of understanding and utilization.
In conclusion, the rate-limiting step in small molecule science is still all too often the synthesis of targeted derivatives. Site-selective functionalization represents a synthetic strategy with an exceptional level of theoretical efficiency, but the challenge in reducing this approach to practice with complex small molecule substrates can be substantial. Electronic tuning can enable the development of highly practical and synthetically useful site-selective functionalization reactions. This strategy may therefore facilitate advanced understanding and harnessing of the still largely untapped functional potential that complex small molecules possess.
Methods
Full experimental details, procedures, and characterization for new compounds are included in the Supplementary Information.
Supplementary Material
Acknowledgements
We gratefully acknowledge M.C. White for helpful discussions and P. Beak for thoughtful review of this manuscript, Bristol-Myers Squibb for a gift of AmB, and the NIH (GM080436) for financial support. M.D.B. is an HHMI Early Career Scientist.
Footnotes
Author Contributions BCW, BEU, and MDB designed experiments. BCW performed acylation experiments. BCW, BEU, GLB, MJC, and TMA contributed to synthesis of intermediates and derivatives. BCW, BEU, and MDB wrote the paper.
The authors declare no competing financial interests. Supplementary information is available for this paper.
1. Lewis CA, Miller SJ. Site-selective derivatization and remodeling of erythromycin A by using simple peptide-based chiral catalysts. Angew. Chem. Int. Ed. 2006;45:5616–5619. [PubMed]
2. Chen MS, White MC. A predictably selective aliphatic C-H oxidation reaction for complex molecule synthesis. Science. 2007;318:783–787. [PubMed]
3. Wender PA, Hilinski MK, Mayweg AVW. Late-stage intermolecular C-H activation for lead diversification: a highly chemoselective oxyfunctionalization of the C-9 position of potent bryostatin analogues. Org. Lett. 2005;7:79–82. [PubMed]
4. Peddibhotla S, Dang Y, Liu JO, Romo D. Simultaneous arming and structure/activity studies of natural products employing O-H insertions: an expedient and versatile strategy for natural products-based chemical genetics. J. Am. Chem. Soc. 2007;129:12222–12231. [PubMed]
5. Snyder SA, Gollner A, Chiriac MI. Regioselective reactions for programmable resveratrol oligomer synthesis. Nature. 2011;474:461–466. [PMC free article] [PubMed]
6. Pathak TP, Miller SJ. Site-selective bromination of vancomycin. J. Am. Chem. Soc. 2012;134:6120–6123. [PMC free article] [PubMed]
7. Afagh NA, Yudin AK. Chemoselectivity and the curious reactivity preferences of functional groups. Angew. Chem. Int. Ed. 2010;49:262–310. [PubMed]
8. Griswold KS, Miller SJ. A peptide-based catalyst approach to regioselective functionalization of carbohydrates. Tetrahedron. 2003;59:8869–8875.
9. Sanchez-Rosello M, Puchlopek AL, Morgan AJ, Miller SJ. Site-selective cataylsis of phenyl thionoformate transfer as a tool for regioselective deoxygenation of polyols. J. Org. Chem. 2008;73:1774–1782. [PubMed]
10. Lewis CA, Merkel J, Miller SJ. Catalytic site-selective synthesis and evaluation of a series of erythromycin analogs. Bioorg. Med. Chem. Lett. 2008;18:6007–6011. [PMC free article] [PubMed]
11. Lewis CA, Longcore KE, Miller SJ, Wender PA. An approach to the site-selective diversification of apoptolidin A with peptide-based catalysts. J. Nat. Prod. 2009;72:1864–1869. [PMC free article] [PubMed]
12. Kurahashi T, Mizutani T, Yoshida J. Functionalized DMAP catalysts for regioselective acetylation of carbohydrates. Tetrahedron. 2002;58:8669–8677.
13. Kawabata T, Furuta T. Nonenzymatic regioselective acylation of carbohydrates. Chem. Lett. 2009;38:640–647.
14. Kurahashi T, Mizutani T, Yoshida J. Effect of intramolecular hydrogen-bonding network on the relative reactivities of carbohydrate OH groups. J. Chem. Soc., Perkin Trans. 1999;1:465–473.
15. Luning U, et al. Concave pyridines for selective acylations of polyols. Eur. J. Org. Chem. 1998:1077–1084.
16. Katting E, Albert M. Counterion-directed regioselective acetylation of octyl β-D-glucopyranoside. Org. Lett. 2004;6:945–948. [PubMed]
17. Gu J, Ruppen ME, Cai P. Lipase-catalyzed regioselective esterification of rapamycin: synthesis of temsirolimus (CCI-779) Org. Lett. 2005;7:3945–3948. [PubMed]
18. Park HG, Do JH, Chang HN. Regioselective enzymatic acylation of multi-hydroxyl compounds in organic synthesis. Biotechnol. Bioprocess Eng. 2003;8:1–8.
19. Hammond GS. A correlation of reaction rates. J. Am. Chem. Soc. 1955;77:334–338.
20. Leffler JE, Grunwald E. Rates and Equilibria of Organic Reactions. Wiley; New York: 1963.
21. Jacobsen EN, Zhang W, Guler ML. Electronic tuning of asymmetric catalysts. J. Am. Chem. Soc. 1991;113:6703–6704.
22. Palucki M, et al. The mechanistic basis for electronic effects on enantioselectivity in the (salen)Mn(III)-catalyzed epoxidation reaction. J. Am. Chem. Soc. 1998;120:948–954.
23. RajanBabu TV, Casalnuovo AL. Role of electronic asymmetry in the design of new ligands: the asymmetric hydrocyanation reaction. J. Am. Chem. Soc. 1996;118:6325–6326.
24. Harper KC, Sigman MS. Three-dimensional correlation of steric and electronic free energy relationships guides asymmetric propargylation. Science. 2011;333:1875–1878. [PubMed]
25. Xu S, et al. The DMAP-catalyzed acetylation of alcohols - a mechanistic study. Chem. Eur. J. 2005;11:4751–4757. [PubMed]
26. Lutz V, et al. Structural analyses of N-acylated 4-(dimethylamino)pyridine (DMAP) salts. Chem. Eur. J. 2009;15:8548–8557. [PubMed]
27. Cheong PH, Legault CY, Um JM, Celebi-Olcum N, Houk KN. Quantum mechanical investigations of organocataysis: mechanisms, reactivities, and selectivities. Chem. Rev. 2011;111:5042–5137. [PMC free article] [PubMed]
28. Palacios DS, Anderson TM, Burke MD. A post-PKS oxidation of the amphotericin B skeleton predicted to be critical for channel formation is not required for potent antifungal activity. J. Am. Chem. Soc. 2007;129:13804–13805. [PMC free article] [PubMed]
29. Palacios DS, Dailey I, Siebert DM, Wilcock BC, Burke MD. Synthesis-enabled functional group deletions reveal key underpinnings of amphotericin B ion channel and antifungal activities. Proc. Natl. Acad. Sci. USA. 2011;108:6733–6738. [PubMed]
30. Gray KC, Palacios DS, Dailey I, Endo MM, Uno BE, Wilcock BC, Burke MD. Amphotericin primarily kills yeast by simply binding ergosterol. Proc. Natl. Acad. Sci. U.S.A. 2012;109:2234–2239. [PubMed]
31. Silberstein A. Conformational analysis of amphotericin B-cholesterol channel complex. J. Membr. Biol. 1998;162:117–126. [PubMed]
32. Baran M, Mazerski M. Molecular modelling of amphotericin B-ergosterol primary complex in water. Biophys. Chem. 2002;95:125–133. [PubMed]
33. Neumann A, Czub J, Baginski M. On the possibility of the amphotericin B-sterol complex formation in cholesterol- and ergosterol-containing lipid bilayers: a molecular dynamics study. J. Phys. Chem. B. 2009;113:15875–15885. [PubMed]
34. Croatt MP, Carreira EM. Probing the role of the mycosamine C2'-OH on the activity of amphotericin B. Org. Lett. 2011;13:1390–1393. [PubMed]
35. Matsumori N, Sawada Y, Murata M. Mycosamine orientation of amphotericin B controlling interaction with ergosterol: sterol-dependent activity of conformation-restricted derivatives with an amino-carbonyl bridge. J. Am. Chem. Soc. 2005;127:10667–10675. [PubMed]
36. De Kruijff B, Gerritsen WJ, Oerlemans A, Demel RA, Van Deenen LLM. Polyene antibiotic-sterol interactions in membranes of Acholeplasma laidlawii cells and lecthin liposomes. III. Molecular structure of the polyene antibiotic-cholesterol complexes. Biochim. Biophys. Acta. 1974;339:57–70. [PubMed]
37. Murata M, et al. Ion channel complex of antibiotics as viewed by NMR. Pure Appl. Chem. 2009;81:1123–1129.
38. Volmer AA, Szpilman AM, Carreira EM. Synthesis and biological evaluation of amphotericin B derivatives. Nat. Prod. Rep. 2010;27:1329–1349. [PubMed]
39. Pandey RC, Rinehart KL. Polyene antibiotics. IX. An improved method for the preparation of methyl esters of polyene antibiotics. J. Antibiotics. 1977;30:158–162. [PubMed]
40. Taylor AW, Costello BJ, Hunter PA, MacLachlan WS, Shanks CT. Synthesis and antifungal selectivity of new derivatives of amphotericin B modified at the C-13 position. J. Antibiotics. 1993;46:486–493. [PubMed]
41. Matsushita N, et al. Synthesis of 25-13C-amphotericin B methyl ester: a molecular probe for solid-state NMR measurements. Chem. Lett. 2009;38:114–115.
42. Jiang L, Chan T. Regioselective acylation of hexopyranosides with pivaloyl chloride. J. Org. Chem. 1998;63:6035–6038. [PubMed]
43. Nicolaou KC, Webber SE. Stereocontrolled total synthesis of lipoxins B. Synthesis. 1986;453
44. Philippe M, et al. New fatty monoesters of erythromycin A. Chem. Pharm. Bull. 1990;38:1672–1674. [PubMed]
45. Hammett LP. Some relations between reaction rates and equilibrium constants. Chem. Rev. 1935;17:125–136.
46. Garegg P, Samuelsson B. Novel reagent system for converting a hydroxy-group into an iodo-group in carbohydrates with inversion of configuration. J. Chem. Soc., Perkin Trans. 1980;1:2866–2869.
47. Hutchins R, et al. Nucleophilic borohydride: selective reductive displacement of halides, sulfonate esters, tertiary amines, and N,N-disulfonimides with borohydride reagents in polar aprotic solvents. J. Org. Chem. 1977;43:2259–2267.
48. Matsumori N, et al. An amphotericin B-ergosterol covalent conjugate with powerful membrane permeabilizing activity. Chem. Biol. 2004;11:673–679. [PubMed]
49. Mayr H, Ofial AR. The reactivity-selectivity principle: An imperishable myth in organic chemistry. Angew. Chem. Int. Ed. 2006;45:1844–1854. [PubMed]
50. Fiori KW, Puchlopek ALA, Miller SJ. Enantioselective sulfonylation reactions mediated by a tetrapeptide catalyst. Nature Chem. 2009;1:630–634. [PMC free article] [PubMed]
51. Jordan PA, Kayser-Bricker KJ, Miller SJ. Asymmetric phosphorylation through catalytic P(III) phosphoramidite transfer: enantioselective synthesis of D-myo-inositol-6-phosphate. Proc. Nat. Acad. Sci. U. S. A. 2010;107:20620–2062. [PubMed]