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Epitope mapping of nonneutralizing HC33 HMAbs. E2 mutant proteins encompassing aa 412 to 423 and aa 434 to 446 were expressed in HEK 293T cells, and cell lysates were analyzed by ELISA. Identification of the contact residue for each antibody was the same as the epitope mapping described for Fig. 2D for the neutralizing HC33 HMAbs.