PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of molcellbPermissionsJournals.ASM.orgJournalMCB ArticleJournal InfoAuthorsReviewers
 
From:

Fig 5

An external file that holds a picture, illustration, etc.
Object name is zmb9991097880005.jpg

Pin1 controls CPEB destruction in mammalian cells. (A) HEK293T cells were transfected with Flag-CPEB, followed by IgG or Pin1 immunoprecipitation and Western analysis for Flag and Pin1. (B) Cells cotransfected with Flag-CPEB and either control GFP vector or GFP-Pin1 were pulse-labeled with [35S]methionine, followed by a chase of radio-inert methionine for the indicated times. The extracts were subjected to Flag immunoprecipitation and analyzed by SDS-PAGE and autoradiography. (C) Cells were cotransfected with Flag-CPEB and either control or Pin1 siRNA followed by pulse-labeling with 35S-methionine and chased with radio-inert methionine for the times indicated, immunoprecipitated for Flag and analyzed by SDS-PAGE and autoradiography. The quantification of 3 experiments is shown on the bottom panel. (D) Cells expressing ectopic WT or W34A or K34A mutant Pin1 proteins (left panel) or R68/69A double mutant Pin1 protein (right panel) were [35S]methionine labeled, followed by a chase of radio-inert methionine, and then immunoprecipitated for Flag-CPEB. (E) Cells expressing ectopic full-length Pin1, Pin1 WW domain, or Pin1 PPIase domain were [35S]methionine labeled, followed by a chase of radio-inert methionine, and then immunoprecipitated for Flag-CPEB. The quantification of three experiments is shown in the right panel.

Images in this article

  • Fig 1
  • Fig 2
  • Fig 3
  • Fig 4
  • Fig 5
  • Fig 6
Click on the image to see a larger version.