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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Int J Cancer. Author manuscript; available in PMC 2012 December 19.
Published in final edited form as:
Int J Cancer. 2011 September 15; 129(6): 1322–1330.
Published online 2011 April 1. doi: 10.1002/ijc.25798

Figure 4

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Aminoacylase expression is up-regulated upon neuronal differentiation of SK-N-SH cells

SK-N-SH cells were arrested by growth in media containing 1% FBS for 12 hours prior to neuronal induction with cAMP. Cells were grown in the absence (A–C) or presence of cAMP (D–F) for 7 days and examined immunocytochemically. Expression of both ACY1 (A, D) and ACY3 (C, F) were robustly expressed in differentiated neurons, as revealed by prominent neurite outgrowth (D′, F′). ASPA was expressed in cells with Schwannian morphology (B, B′), but not in arrested cells with neurite-like processes (B, B′ arrows). In contrast, ASPA was expressed in neurons (E, E′ arrows), particularly at points of cell-cell contact (E, E′ arrowheads), in response to cAMP treatment. Scale bar = 100 μm, inset = 50 μm.

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