Aminoacylase expression was minimally altered when differentiated in medium containing 10% serum
SK-N-SH (A) and SMS-KCNR (B) cells were maintained in growth media containing 10% FBS in the absence or presence of differentiating agents for 7 days and examined immunocytochemically. Centriolar ACY1 expression (AA) was lost under neuronal (AE, I) and Schwannian (AM) differentiation conditions. ASPA expression was unaltered, with the exception of decreased expression in the presence of cAMP (AJ). ACY3 expression was more dramatically altered under neuronal (AG, K) than Schwannian (AO) differentiation conditions. Phase-contrast images revealed changes toward neuronal (i.e. process outgrowth) and Schwannian (i.e., larger cell size) cellular morphology. SMS-KCNR cells (B) failed to exhibit profound morphological alterations upon differentiation. Scale bar = 100 μm.