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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
World J Surg. Author manuscript; available in PMC 2012 December 13.
Published in final edited form as:
PMCID: PMC3521612
NIHMSID: NIHMS419030

Rate of Clinically Significant Postoperative Pancreatic Fistula in Pancreatic Neuroendocrine Tumors

Abstract

Background

In 2005, the International Study Group of Pancreatic Fistula (ISGPF) developed a definition and grading system for postoperative pancreatic fistula (POPF). The authors sought to determine the rate of POPF after enucleation and/or resection of pancreatic neuroendocrine tumors (PNET) and to identify clinical, surgical, or pathologic factors associated with POPF.

Methods

A retrospective analysis of pancreatic enucleations and resections performed from March 1998 to April 2010. We defined a clinically significant POPF as a grade B that required nonoperative intervention and grade C.

Results

One hundred twenty-two patients were identified; 62 patients had enucleations and 60 patients had resections of PNET. The rate of clinically significant POPF was 23.7 % (29/122). For pancreatic enucleation, the POPF rate was 27.4 % (17/62, 14 grade B, 3 grade C). The pancreatic resection group had a POPF rate of 20 % (12/60, 10 grade B, 2 grade C). This difference was not significant (p = 0.4). In univariate analyses, patients in the enucleation group with hereditary syndromes (p = 0.02) and non-insulinoma tumors (p = 0.02) had a higher POPF rate. Patients in the resection group with body mass index (BMI) >25 (p <0.01), multiple endocrine neoplasia type 1 (MEN-1; p <0.01) and those who underwent simultaneous multiple procedures (p = 0.02) had a higher POPF rate. Multivariate analyses revealed that hereditary syndromes were able to predict POPF in the enucleation group, while having BMI >25 and increasing lesion size were also associated with POPF in the group undergoing resection.

Conclusions

We found a clinically significant POPF rate after surgery in PNET to be 23.7 % with no difference by the type of operation. Our POPF rate is comparable to that reported in the literature for pancreatic resection for other types of tumors. Certain inherited genetic diseases—von Hippel–Lindau disease (VHL) and MEN-1—were associated with higher POPF rates.

Introduction

Postoperative pancreatic fistula (POPF) is a well-described complication after pancreatic surgery; it is associated with significant morbidity and occurs in up to 50 % of patients [17]. Prior to the recent establishment of a universal definition, it was difficult to compare POPF rates published in the literature. In 2005, the International Study Group of Pancreatic Fistula (ISGPF) developed a universal objective definition and grading system for the severity of POPF [3]. A uniform definition provides an opportunity to accurately compare POPF rates among different institutions, surgical techniques, surgical procedures, histologies, and clinical factors. Since the ISGPF definition has been established, most of the published data for POPF focuses on pancreatic resection for pancreatic adenocarcinoma with little focus on pancreatic neuroendocrine tumors (PNET) or pancreatic enucleation. The rates of POPF, using the ISGPF criteria, range from 17 to 39 % [812].

Pancreatic neuroendocrine tumors are rare, representing only 1–2 % of all pancreatic neoplastic lesions [13]. The incidence of PNET is approximately 1 per 100,000 persons per year in the general population [13, 14]. Pancreatic neuroendocrine tumors arise from endocrine pancreatic cells or the islets of Langerhans, unlike pancreatic adenocarcinomas, which make up >95 % of pancreatic tumors and arise from the exocrine or ductal cells of the pancreas [1517]. Pancreatic neuroendocrine tumors are usually well-differentiated, slow growing, and, if malignant, carry a better prognosis than pancreatic adenocarcinomas [14]. The majority of PNET are sporadic; however about 10–15 % are associated with inherited genetic diseases such as MEN-1, VHL, neurofibromatosis (NF), and tuberous sclerosis (TSC) [18]. Up to 17 % of VHL patients [19, 20] and up to 55.4 % of MEN-1 patients will develop a PNET [21].

Surgery is the cornerstone of therapy for patients with PNET. Surgery is the only potentially curative treatment available and is indicated for patients with a PNET to control hormone hypersecretion, local compressive symptoms, malignancy, and for palliation of symptoms in patients with advanced disease. Parenchymal sparing techniques such as pancreatic enucleation as well as the utilization of laparoscopic technology have shown similar long-term survival compared to resection and laparotomy in patients with PNET [22]. Pancreatic enucleation of small local PNET has been the surgical paradigm. Limited data have been published evaluating the impact of surgical technique on the development of POPF in PNET.

The purpose of the present study was to determine the rate of clinically significant POPF for PNET and to evaluate if a difference exists in POPF rate by surgical technique—pancreatic enucleation versus resection. The study also sought to identify any clinical, surgical, or pathologic factors that may be associated with an increased risk for the development of POPF.

Methods

A retrospective single-institution review was performed on all patients with PNET who underwent either pancreatic resection or enucleation from March 1998 to April 2010. One hundred twenty-two patients with PNET underwent 122 surgical procedures at the National Institutes of Health Clinical Center, Bethesda, MD. This study was approved by the Office of Human Subject Research at the National Institutes of Health. All participants provided informed written consent.

Variables evaluated for their association with development of POPF included age, gender, preoperative serum albumin level, body mass index (BMI) classification (kg/m2) (normal ≤24.9, overweight 25–29.9, obese ≥30.0), if the tumor was functional or non-functional, and if the lesion was sporadic or, in the setting of an inherited genetic disease, known to be associated with increased risk of PNET. The PNET in patients with symptoms and biochemical evidence of hormone excess preoperatively were classified with standard techniques and diagnosed as functional tumors [15, 16, 2329]. As much as 50–60 % of PNET are functionally active and secrete hormones in excess, including insulin, gastrin, somatostatin, vasoactive intestinal peptide (VIP), and, more rarely, adrenocorticotropic hormone (ACTH), and luteinizing hormone (LH) [15]. If there were no clinical symptoms of hormonal excess, and if plasma hormone levels were normal, the PNET was classified as non-functional. Patients with inherited genetic diseases, such as MEN-1 and VHL, were diagnosed based on clinical presentation, family history, and/or genetic testing [30]. All patients with MEN-1 were genetically tested for a mutation in the MEN1 gene unless the patient was known to belong to a kindred with prior testing and had at least two clinical manifestations. Patients with VHL were tested for a mutation in the VHL gene.

All operative reports were reviewed in order to precisely determine the type of procedure performed and location of the lesions. An intraoperative ultrasound of the pancreas was routinely performed to assess the distance to the main pancreatic duct and evaluate the parenchyma for any additional lesions. All patients had a closed suction intra-operative drain placed at the pancreatic anastomosis, resection margin, or enucleation bed. Pathology reports were likewise reviewed to determine histology and confirm the size and number of lesions resected or enucleated.

The postoperative course for all patients was reviewed in order to determine the rate and appropriate classification of POPF. All patients’ drain amylase levels were measured postoperatively. The ISGPF definition of drain amylase >3 times the normal serum amylase value on or after postoperative day 3 was used to determine POPF [3]. From strict ISGPF criteria, POPF were categorized as follows: grade A, an asymptomatic fistula with no interventions required, only elevated amylase from the drain fluid; grade B, a symptomatic fistula requiring a postoperative drain for >21 days, nonoperative intervention such as total parenteral nutrition (TPN), octreotide, percutaneous drain, or readmission within 30 days of hospitalization; and grade C, a severe fistula requiring surgical and nonoperative intervention, including reoperation, intensive care unit (ICU) care >48 h, or death within 30 days of operation. Our patients did not routinely receive perioperative octreotide as prophylaxis for the prevention of POPF.

Patient progress notes, discharge summaries, and clinic notes were used to determine the length of time the postoperative drain was in place and if any nonoperative interventions were required. Postoperative interventions evaluated included antibiotic use beyond the 24 h perioperative period, presence of an infection confirmed with microbiology culture, blood product transfusion requirements, use of TPN or octreotide, imaging with computed tomography (CT), percutaneous drain placement, and any other postoperative complication.

Occasionally patients coming from outside the greater Washington DC area were discharged home with a surgical drain in place and returned to clinic between 3 and 6 weeks after surgery for their first postoperative check and possible surgical drain removal. Secondary to the time of their first postoperative clinic visit, some patients were placed in the grade B fistula category only because the surgical drain was kept in place for greater than 21 days. These patients did not meet any other criteria for a grade B POPF because they did not require nonoperative intervention, diet modification, readmission, or have prolonged hospital stays. These patients are similar to patients with asymptomatic grade A POPF. A recent study indicated that grade B and C POPF were clinically significant because they are associated with increased length of hospital stay and added cost [31]. In order to accurately report clinically significant POPF in our patient population, we divided the grade B patients into two cohorts for subgroup analysis: grade B1 patients were asymptomatic, did not require nonoperative interventions and only had a surgical drain in place >21-days because of travel constraints; grade B2 patients had symptomatic, clinically significant POPF, that required nonoperative interventions and had a surgical drain in place >21 days.

We analyzed the type of pancreatic procedure: enucleation or resection. Patients who underwent a pancreatectomy and enucleation in the same procedure were placed in the pancreatic resections group. An initial screening was performed in which Fisher’s exact test was used to compare the frequencies of dichotomous clinical parameters according to each of the two classification groups, while the distribution of each of the continuous parameters was compared between the two groups of interest using an exact Wilcoxon rank sum test. Extent of surgery was compared between the two groups using a Cochran–Armitage test for trend. Any parameters found to have possible association with development of a fistula, as exhibited by having univariate p values <0.10 from the screening procedure, were further evaluated jointly in a multivariate logistic regression model when appropriate. All p values are two-tailed and univariate p values have not been formally adjusted for multiple comparisons. However, in view of the number of tests performed, only p values less than 0.01 should be interpreted as statistically significant. All data presented are median ± standard deviation except where otherwise specified.

Results

One hundred twenty-two patients had operations for PNET; 62 underwent pancreatic enucleation and 60 underwent pancreatic resection. The median age at the time of surgery was 42 years (range 11–78 years) with a slight female predominance 58.2 % (71/122). The patients had a median body mass index (BMI) of 28.7 kg/m2 and a preoperative serum albumin level of 3.9 g/dL. Some 52.5 % (64/122) of the patients had an inherited genetic disease, and 63.1 % (77/122) had functional tumors (Table 1). There were 28 patients with MEN-1, 34 with VHL, one with neurofibromatosis (NF), and one with Birt–Hogg–Dubé syndrome. Our study had 53 insulinomas, 16 gastrinomas, one VIPoma, one somatostatinoma; one corticotropin releasing hormone (CRH) producing lesion, one adrenocorticotropic hormone (ACTH) producing lesion, one luteinizing hormone (LH) producing lesion, and one ectopic pheochromocytoma; one accessory spleen in a patient with a history of prior PNET; one pancreatic acinar carcinoma in a patient with VHL; and two microcystic adenomas in patients with VHL. There were 43 patients with nonfunctional PNET (Table 2).

Table 1
Demographics and clinical characteristics of study cohort
Table 2
Types of pancreatic neuroendocrine tumors (PNET)

The pancreatic enucleation and resection group were analyzed separately with the ISGPF criteria for POPF [3] (Table 3). The postsurgical outcomes data used to classify patients based on ISGPF criteria are shown in (Table 4). The 30 day mortality was less than 1 % (1/122 patients), and 6.6 % (8/122 patients) required a reoperation.

Table 3
Pancreatic fistula grade criteria suggested by Study Group of Pancreatic Fistula (IGSPF)
Table 4
Post surgical outcomes

The 62 patients underwent pancreatic enucleation of 83 lesions. During a single procedure, 51 patients had one enucleation, 3 patients had two enucleations, 7 patients had three enucleations, and 1 patient had more than five lesions enucleated. Nine patients underwent a laparoscopic procedure and 53 patients underwent a laparotomy. The largest lesion was enucleated from the head of the pancreas in 42 patients and from the body or tail of the pancreas in 20 other patients (Table 5). The median size of the largest lesion enucleated was 1.8 ± 0.74 cm.

Table 5
Pancreatic enucleation

Sixty patients underwent a pancreatic resection for their PNET. Thirteen patients underwent a laparoscopic procedure and 47 underwent a laparotomy. These patients received a variety of pancreatic resections, including 39 distal pancreatectomy, 15 pancreaticoduodenectomy, 3 subtotal pancreatectomy, 1 total pancreatectomy, 2 pancreaticoduodenectomy and distal pancreatectomy. Of these 60 patients, 2 patients had simultaneous pancreaticoduodenectomy and distal pancreatectomy and 10 had a combined pancreatectomy and enucleation (Table 6). The median size of the largest lesion resected was 3.2 ± 2.5 cm.

Table 6
Pancreatic resection

In the enucleation group, the POPF were graded as follows: 25.8 % (16/62) grade A, 14.5 % (9/62) grade B1, 22.6 % (14/62) grade B2, and 4.8 % (3/62) grade C. Grade B and C POPF was found in 26 of 62 patients. Clinically significant POPF occurred in 14 patients with grade B2 and 3 patients with grade C, for a total rate of 27.4 % (17/62) (Table 7). A univariate analysis was done evaluating age, gender, albumin, BMI, type of procedure, number of lesions, size of largest lesion, location of lesion, histology, functionality of PNET, and inherited genetic diseases. Non-insulinoma tumors (p = 0.02) and inherited genetic diseases (p = 0.02) were associated with a higher POPF rate (Table 8). Based on the p <0.10 threshold for evaluating parameters in a multivariate logistic model, histology, number of lesions, and inherited genetic disease type were considered as variables to be potentially evaluated relative to their association with development of a fistula. With a backward selection algorithm, it was determined that only inherited genetic diseases would be associated with fistula. This model was able to correctly classify 17/20 (85 %) patients without a fistula but only 20/42 with a fistula (48 %). Most patients (38/40) with insulinoma were sporadic, whereas almost all patients (21/22) with non-insulinoma tumors had an inherited disease and had a 1.5 times higher rate of POPF.

Table 7
Pancreatic fistula rates
Table 8
Univariate analysis of factors associated with postoperative pancreatic fistula (POPF)

For the pancreatic resection group, the POPF were graded: 18.3 % (11/60) grade A, 18.3 % (11/60) grade B1, 16.7 % (10/60) grade B2, and 3.3 % (2/60) grade C. Grade B and C POPF was found in 23 of 60 patients. Clinically significant POPF occurred in 10 patients with grade B2 and 2 patients with gade C, for a total rate of 20 % (12/60 patients) (Table 7). A univariate analysis was conducted evaluating age, gender, albumin, BMI, transfusion, type of procedure, number of lesions, size of largest lesion, histology, functionality of PNET, and inherited genetic diseases. A BMI >25 (p <0.01), MEN-1 (p <0.01), and combined pancreatic resections and enucleation or simultaneous pancreaticoduodenectomy and distal pancreatectomy (p = 0.02) were associated with a higher POPF rate (Table 8). Those with a fistula also tended to have greater maximal lesion size (mean ± SEM = 3.75 ± 0.53 vs. 2.40 ± 0.20; p = 0.067), as well as larger actual BMI (31.79 ± 1.38 vs. 26.44 ± 1.01; p = 0.011). Using the p = 0.10 threshold from the screening procedure to determine which parameters to include in a multivariate logistic regression model, BMI, MEN-1 or not, VHL or not, extent of surgery (combined pancreatic resections and enucleation or simultaneous pancreaticoduodenectomy and distal pancreatectomy), and maximum lesion size were all considered for inclusion in such a model. Based on a backward selection algorithm, a model with actual maximal lesion size and whether BMI was normal or not could be used to predict who would or would not have a fistula in the resection group.

Specifically, the model resulted in the following classification rule:

Classifytofistulaif-3.1075(ifBMIisnormal;0ifnot)+0.4877×max.lesionsize1.0.Classifytonofistulaif-3.1075(ifBMIisnormal;0ifnot)+0.4877×max.lesionsize<1.0.

Applying these rules to the data from which they were derived results in the following classification: 16/22 (68 %) without a fistula would be correctly identified, whereas 20/32 (63 %) with a fistula would be correctly identified. Further analysis of each surgical subgroup (enucleation and resection) was performed to identify if any surgical, clinical, or pathologic factors were predictive of severity of POPF. Risk factors were associated with the development of POPF but did not correlate with severity (grade A, B, or C) of fistula that developed. If patients who underwent combined procedures are excluded from the pancreatic resection group, there is still no difference in POPF between the groups. The POPF rate is 23 of 48 in the resection group and 26 of 62 in the enucleation group (p = 0.57).

Our clinically significant POPF rate for all procedures performed on PNET was 23.7 % (29/122). In the 122 patients, we identified 24 (16.4 %) grade B2 fistulas (14 enucleations and 10 resections) and 5 (4.1 %) grade C fistulas (3 enucleations and 2 resections). Patients who underwent pancreatic enucleation had a clinically significant POPF rate of 27.4 % (17/62), and those who underwent pancreatic resection had a POPF rate of 20 % (12/60). The difference in POPF was not significant (p = 0.4). Patient characteristics were analyzed separately by type of operation (resection vs. enucleation) (Table 8).

Discussion

Surgery is the only potentially curative option for PNET. Pancreatic fistula remains the most significant cause of postoperative morbidity and occasionally mortality in these patients who undergo pancreatic surgery. It is critical to elucidate risk factors that can predispose to severe fistula formation in this relatively rare inhomogeneous group. Our clinically significant POPF rate for all procedures performed on PNET was 23.7 % (29/122) with no difference between pancreatic resection and enucleation.

Because of our institutions’ referral bias, we treat a large number of patients with MEN-1 and VHL. Our study showed a higher rate of POPF in patients with MEN-1 in the pancreatic resection group and VHL and MEN-1 patients in the pancreatic enucleation group. MEN-1 is an autosomal dominant syndrome associated with mutations in the MEN1 gene, a tumor suppressor. Patients develop multiple neuroendocrine tumors within the pancreas, pituitary, and parathyroid glands and present with multiple PNET [23]. Von Hippel–Lindau disease is an autosomal dominant syndrome associated with mutations in the VHL tumor suppressor gene, which predisposes patients to neoplasms in a variety of organs, including the kidney (renal cell carcinoma), the adrenal gland (pheochromocytoma), the central nervous system (hemangioblastomas), the eye (retinal angioma), the inner ear (endolymphatic sac neoplasm), the epididymis (epididymal cystadenoma), and the pancreas (cystic lesions, cystic or serous cystadenomas, and PNET) [19]. Patients with MEN-1 and VHL have an increased risk for the development of PNET [1921, 32]. Secondary to their increased risk of PNET, these patients undergo routine pancreatic computed tomography screening at our institution under an established clinical protocol. Patients with VHL and MEN-1 not only can have multiple PNET, but VHL patients frequently have multiple cystic lesions in their pancreas [1821, 33]. Their abnormal pancreatic parenchyma secondary to their underlying genetic defects and may contribute to a higher rate of POPF [1821, 33].

In the pancreatic enucleation group, patients with non-insulinoma tumors had a higher POPF rate. However, 21 of 22 patients with non-insulinoma tumors had an inherited disease (VHL or MEN-1). It is difficult to ascertain if the non-insulinoma tumors, underlying genetic disease, or both account in part for the higher POPF rate, since 21 of 22 patients had both non-insulinoma tumors and an inherited genetic disease. Eleven patients underwent enucleation of more than one lesion during a single procedure. Enucleation of more than one lesion during a single procedure had a trend toward increased POPF rate but was not a factor found to be significant (p = 0.09). Most of these patients (9/11) had an inherited genetic disease, and that may be a contributing factor to the slightly higher POPF rate.

Patients in the pancreatic resection group with a BMI >25 and with larger lesions had an increased risk for the development of POPF. Body mass index >25 has been documented in the literature, for adenocarcinoma, as a risk factor for POPF [34]. However, BMI was not associated with POPF in the enucleation group.

A recent report [10] suggests that PNET are a risk factor for developing POPF when compared to procedures for pancreatic adenocarcinoma or chronic pancreatitis. These investigators found an increased risk for POPF with enucleation compared to resection. A previous study evaluated enucleation versus resection in small—less than 3 cm—peripancreatic or PNET and found a higher POPF rate in the enucleation group but no difference in the rate of severe POPF (grade B and C) between groups [12]. This is consistent with our data that show a clinically significant POPF (grade B2 and C) for enucleation of 27.4 % compared to 20 % in the resection group (p = 0.4), which is not statistically different.

Given the overall POPF rate in patients with PNET, it appears prudent that all patients have drains placed at the time of surgery. Our results are unlikely to change our practice of leaving a drain in every patient at the time of surgery. The data regarding octreotide as prophylaxis are conflicting; however, in patients with increased risk such as inherited genetic diseases such as MEN or VHL, BMI >25, non-insulinoma histology or combined pancreatic resection and enucleation, octreotide may be considered [35].

Data were collected on the surgical technique used to transect the pancreatic parenchyma in both the enucleation group and the resection group. There was a wide variety of techniques used, including electrocautery, harmonic scalpel, several different stapling devices, blunt dissection, and a combination of two or more. Various techniques were also used to close the pancreatic remnant in the distal pancreatectomies. Because of the retrospective nature of our study and varying surgical details, it is impossible to know what role these various techniques may have played in the POPF rate.

Other surgical techniques to reduce POPF have been described in the literature, such as the use of a Roux-en-Y limb to the enucleation bed or to the pancreatic margin in distal pancreatectomies [36]. Our institution has not employed these techniques, but they are worth considering in an attempt to decrease the POPF rate in both the resection and enucleation groups in patients with increased risk of POPF formation.

The overall POPF rate is relatively high in our patient population, all of whom underwent a pancreaticoduodenectomy, compared to the literature [812]. In our patients who underwent pancreaticoduodenectomy, 50 % (7/14) developed POPF, but 21.4 % (3/14) developed clinically significant POPF. The high overall POPF rate is likely due to the soft pancreatic gland and non-dilated pancreatic duct in our patients. These characteristic have been shown to increase POPF after pancreaticoduodenectomy in patients with pancreatic adenocarcinoma [3739].

Additionally, exact surgical technique used in the pancreaticojejunostomy anastomosis (duct to mucous vs. invagination technique) varied among surgeons and is a factor that could not be analyzed or factored into any risk model secondary to the small number of pancreaticoduodenectomies performed in our series [37, 40]. Regardless, the rate of clinically significant POPF in this series is similar to published literature for adenocarcinoma [812]. Pancreatic invagination technique for the pancreaticojejunostomy anastomosis could be considered in high-risk patients in the future.

Conclusions

The goal of the present study was to evaluate the clinically significant postoperative pancreatic fistula rates in patients with PNET following pancreatic enucleation and resection. Using the ISGPF criteria as a guide, we found the clinically significant rate of POPF for PNET of 23.7 %. There was no difference in clinically significant POPF rate between enucleation (27.4 % [17/62]) and resection (20 % [12/60]) (p = 0.4).

Patients with inherited diseases had a higher rate of POPF in both the enucleation and resection groups. Patients with inherited diseases may have a higher POPF rate because of their underlying abnormal pancreatic parenchyma. Patients with VHL frequently have multiple cystic lesions in their pancreas, and those with MEN-1 usually have multiple microscopic PNET [1821, 33].

Contributor Information

Suzanne M. Inchauste, Endocrine Oncology Section, Surgery Branch, Center for Cancer Research, National Cancer Institute, 10 Center Drive, MSC1201, Rm 4W-5940, Bethesda, MD 20892-1201, USA.

Brock J. Lanier, Endocrine Oncology Section, Surgery Branch, Center for Cancer Research, National Cancer Institute, 10 Center Drive, MSC1201, Rm 4W-5940, Bethesda, MD 20892-1201, USA.

Steven K. Libutti, Department of Surgery, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY, USA.

Giao Q. Phan, Endocrine Oncology Section, Surgery Branch, Center for Cancer Research, National Cancer Institute, 10 Center Drive, MSC1201, Rm 4W-5940, Bethesda, MD 20892-1201, USA.

Naris Nilubol, Endocrine Oncology Section, Surgery Branch, Center for Cancer Research, National Cancer Institute, 10 Center Drive, MSC1201, Rm 4W-5940, Bethesda, MD 20892-1201, USA.

Seth M. Steinberg, Biostatistics and Data Management Section, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.

Electron Kebebew, Endocrine Oncology Section, Surgery Branch, Center for Cancer Research, National Cancer Institute, 10 Center Drive, MSC1201, Rm 4W-5940, Bethesda, MD 20892-1201, USA.

Marybeth S. Hughes, Endocrine Oncology Section, Surgery Branch, Center for Cancer Research, National Cancer Institute, 10 Center Drive, MSC1201, Rm 4W-5940, Bethesda, MD 20892-1201, USA.

References

1. Cameron JL, Pitt HA, Yeo CJ, et al. One hundred forty-five consecutive pancreaticoduodenectomies without mortality. Ann Surg. 1993;217:430–435. (discussion 435–438) [PubMed]
2. Yeo CJ, Cameron JL, Sohn TA, et al. Six hundred fifty consecutive pancreaticoduodenectomies in the 1990s: pathology, complications, and outcomes. Ann Surg. 1997;226:248–257. (discussion 257–260) [PubMed]
3. Bassi C, Dervenis C, Butturini G, et al. Postoperative pancreatic fistula: an international study group (ISGPF) definition. Surgery. 2005;138:8–13. [PubMed]
4. Buchler MW, Friess H, Wagner M, et al. Pancreatic fistula after pancreatic head resection. Br J Surg. 2000;87:883–889. [PubMed]
5. Kazanjian KK, Hines OJ, Eibl G, et al. Management of pancreatic fistulas after pancreaticoduodenectomy: results in 437 consecutive patients. Arch Surg. 2005;140:849–854. (discussion 846–854) [PubMed]
6. Gouma DJ, van Geenen RC, van Gulik TM, et al. Rates of complications and death after pancreaticoduodenectomy: risk factors and the impact of hospital volume. Ann Surg. 2000;232:786–795. [PubMed]
7. Phan GQ, Yeo CJ, Hruban RH, et al. Surgical experience with pancreatic and peripancreatic neuroendocrine tumors: review of 125 patients. J Gastrointest Surg. 1998;2:473–482. [PubMed]
8. Dong X, Zhang B, Kang MX, et al. Analysis of pancreatic fistula according to the International Study Group on Pancreatic Fistula classification scheme for 294 patients who underwent pancreaticoduodenectomy in a single center. Pancreas. 2011;40:222–228. [PubMed]
9. Tan WJ, Kow AW, Liau KH. Moving towards the New International Study Group for Pancreatic Surgery (ISGPS) definitions in pancreaticoduodenectomy: a comparison between the old and new. HPB (Oxf) 2011;13:566–572. [PubMed]
10. Fendrich V, Merz MK, Waldmann J, et al. Neuroendocrine pancreatic tumors are risk factors for pancreatic fistula after pancreatic surgery. Dig Surg. 2011;28:263–269. [PubMed]
11. Hackert T, Werner J, Buchler MW. Postoperative pancreatic fistula. Surgeon. 2011;9:211–217. [PubMed]
12. Pitt SC, Pitt HA, Baker MS, et al. Small pancreatic and periampullary neuroendocrine tumors: resect or enucleate? J Gastrointest Surg. 2009;13:1692–1698. [PMC free article] [PubMed]
13. Halfdanarson TR, Rabe KG, Rubin J, et al. Pancreatic neuroendocrine tumors (PNETs): incidence, prognosis and recent trend toward improved survival. Ann Oncol. 2008;19:1727–1733. [PMC free article] [PubMed]
14. Wang DS, Zhang DS, Qiu MZ, et al. Prognostic factors and survival in patients with neuroendocrine tumors of the pancreas. Tumour Biol. 2011;32:697–705. [PubMed]
15. Kloppel G. Tumour biology and histopathology of neuroendocrine tumours. Best Pract Res Clin Endocrinol Metab. 2007;21:15–31. [PubMed]
16. Kloppel G, Anlauf M, Perren A. Endocrine precursor lesions of gastroenteropancreatic neuroendocrine tumors. Endocr Pathol. 2007;18:150–155. [PubMed]
17. Pour PM, Schmied B. The link between exocrine pancreatic cancer and the endocrine pancreas. Int J Pancreatol. 1999;25:77–87. [PubMed]
18. Oberg K, Eriksson B. Endocrine tumours of the pancreas. Best Pract Res Clin Gastroenterol. 2005;19:753–781. [PubMed]
19. Blansfield JA, Choyke L, Morita SY, et al. Clinical, genetic and radiographic analysis of 108 patients with von Hippel–Lindau disease (VHL) manifested by pancreatic neuroendocrine neoplasms (PNETs) Surgery. 2007;142:814–818. (discussion 818, e811–e812) [PubMed]
20. Binkovitz LA, Johnson CD, Stephens DH. Islet cell tumors in von Hippel–Lindau disease: increased prevalence and relationship to the multiple endocrine neoplasias. Am J Roentgenol. 1990;155:501–505. [PubMed]
21. Goudet P, Murat A, Binquet C, et al. Risk factors and causes of death in MEN1 disease. A GTE (Groupe d’Etude des Tumeurs Endocrines) cohort study among 758 patients. World J Surg. 2010;34:249–255. doi: 10.1007/s00268-009-0290-1. [PubMed] [Cross Ref]
22. DiNorcia J, Lee MK, Reavey PL, et al. One hundred thirty resections for pancreatic neuroendocrine tumor: evaluating the impact of minimally invasive and parenchyma-sparing techniques. J Gastrointest Surg. 2010;14:1536–1546. [PubMed]
23. Kulke MH, Bendell J, Kvols L, et al. Evolving diagnostic and treatment strategies for pancreatic neuroendocrine tumors. J Hematol Oncol. 2011;4:29. [PMC free article] [PubMed]
24. Roth E, Muhlbacher F, Karner J, et al. Free amino acid levels in muscle and liver of a patient with glucagonoma syndrome. Metabolism. 1987;36:7–13. [PubMed]
25. Whipple AO, Frantz VK. Adenoma of islet cells with hyperinsulinism: a review. Ann Surg. 1935;101:1299–1335. [PubMed]
26. Jensen RT. Gastrointestinal endocrine tumours. Gastrinoma. Baillieres Clin Gastroenterol. 1996;10:603–643. [PubMed]
27. Jensen RT, Niederle B, Mitry E, et al. Gastrinoma (duodenal and pancreatic) Neuroendocrinology. 2006;84:173–182. [PubMed]
28. Zollinger RM. Islet cell tumors: a diarrheogenic syndrome. Prensa Med Argent. 1971;58:1458–1462. [PubMed]
29. Zollinger RM, Ellison EH. Primary peptic ulcerations of the jejunum associated with islet cell tumors of the pancreas. Ann Surg. 1955;142:709–723. (discussion 708–724) [PubMed]
30. Batcher E, Madaj P, Gianoukakis AG. Pancreatic neuroendocrine tumors. Endocr Res. 2011;36:35–43. [PubMed]
31. Pratt WB, Maithel SK, Vanounou T, et al. Clinical and economic validation of the International Study Group of Pancreatic Fistula (ISGPF) classification scheme. Ann Surg. 2007;245:443–451. [PubMed]
32. Imamura M. Recent standardization of treatment strategy for pancreatic neuroendocrine tumors. World J Gastroenterol. 2010;16:4519–4525. [PMC free article] [PubMed]
33. Oberg K. Neuroendocrine tumors of the gastrointestinal tract: recent advances in molecular genetics, diagnosis, and treatment. Curr Opin Oncol. 2005;17:386–391. [PubMed]
34. House MG, Fong Y, Arnaoutakis DJ, et al. Preoperative predictors for complications after pancreaticoduodenectomy: impact of BMI and body fat distribution. J Gastrointest Surg. 2008;12:270–278. [PubMed]
35. Vanounou T, Pratt WB, Callery MP, et al. Selective administration of prophylactic octreotide during pancreaticoduodenectomy: a clinical and cost–benefit analysis in low- and high-risk glands. J Am Coll Surg. 2007;205:546–557. [PubMed]
36. Wagner M, Gloor B, Ambuhl M, et al. Roux-en-Y drainage of the pancreatic stump decreases pancreatic fistula after distal pancreatic resection. J Gastrointest Surg. 2007;11:303–308. [PubMed]
37. Berger AC, Howard TJ, Kennedy EP, et al. Does type of pancreaticojejunostomy after pancreaticoduodenectomy decrease rate of pancreatic fistula? A randomized, prospective, dual-institution trial. J Am Coll Surg. 2009;208:738–747. (discussion 737–739) [PubMed]
38. DeOliveira ML, Winter JM, Schafer M, et al. Assessment of complications after pancreatic surgery: a novel grading system applied to 633 patients undergoing pancreaticoduodenectomy. Ann Surg. 2006;244:931–937. (discussion 937–939) [PubMed]
39. Lin JW, Cameron JL, Yeo CJ, et al. Risk factors and outcomes in postpancreaticoduodenectomy pancreaticocutaneous fistula. J Gastrointest Surg. 2004;8:951–959. [PubMed]
40. Zhu B, Geng L, Ma YG, et al. Combined invagination and duct-to-mucosa techniques with modifications: a new method of pancreaticojejunal anastomosis. Hepatobiliary Pancreatic Dis Int. 2011;10:422–427. [PubMed]