A 68-year-old woman was admitted to our department in Januray 2012 because of epigastric pain for half a month. She had no fever, no nausea or vomiting, no hematemesis or melena, and no weight loss. She underwent open cholecystectomy for gallbladder stones 25 years ago. Physical examination on admission showed no abnormalities. Blood biochemistry and tumor markers (carcinoembryonic antigen, alpha fetoprotein, carbohydrate antigen 19-9 carbohydrate antigen 153, carbohydrate antigen 125) were all within normal ranges. Computed tomography (CT) of the abdomen with intravenous contrast revealed cystic dilatation of left intrahepatic bile ducts accompanied with stones (Figure ) and multiple cystic lesions in the pancreatic tail (Figure ). Magnetic resonance cholangiopancreato-graphy revealed a diffuse dilated biliary tract with multiple filling defects in the left lateral lobe and segmental cystic dilatation of pancreatic duct in the pancreatic tail (Figure ). According to the medical history and the imaging findings, left intrahepatic bile duct stones and pancreatic IPMN was first considered. Therefore, laparoscopic left lateral hepatolobectomy and spleen-preserving distal pancreatectomy were performed.
Computed tomography scan. A: A cystic dilation of the left intrahepatic bile ducts accompanied with stones; B: Multiple cystic lesions in the pancreatic tail.
Magnetic resonance cholangiopancreatography reveals diffuse dilation of biliary tract with multiple filling defects in the left lateral lobe of the liver and segmental cystic dilation of pancreatic duct in the pancreatic tail.
The patient was placed in supine position under general anesthesia. The operator and the second assistant who held the laparoscope stood on the right side of the patient and the first assistant stood on the left. Carbon dioxide pneumoperitoneum was established using a Veress needle to a pressure of 15 mmHg. One initial 10-mm trocar was placed for laparoscope below the umbilicus and another four trocars (one of 12 mm and three of 5 mm) were inserted into the left upper, left flank, right upper, and right flank quadrants, making the five trocars arrange in a V-shape (Figure ). During laparoscopic exploration, severe adhesion in the right upper abdomen because of the former open cholecystectomy and the atrophy of left lateral lobe of the liver were found, but without abdominal metastasis.
The location of trocars placement.
After dissecting the adhesion, the gastrocolic omentum was divided for entrance to the lesser sac by ultrasonic coagulating shears (Harmonic Ace scalpel, Ethicon Endo-Surgery, Inc., Cincinnati, OH, United States). The stomach was reflected cephalad and the tumor was found in the tail of pancreas. According to the location of the tumor, the mobilization of the pancreas began at the upper border till the common hepatic artery and the proximal splenic artery were visualized. Then the pancreas was mobilized dorsally starting at the lower edge to visualize the splenic vein. Pancreas was transected 2 cm proximal to the right side of the tumor with an endoscopic linear staplers (Endocutter 60 staple, white cartridge; Ethicon, Endo-Surgery, Inc., Cincinnati, OH, United States) and the pancreatic tail was freely dissected from the splenic artery and vein by ligation of the small branches connected to the pancreas using small titanium vascular clips or ultrasonic coagulating shears.
The left lateral lobe of the liver was mobilized by dissecting the round, falciform, left coronary and triangular ligaments. Liver parenchyma was also divided by ultrasonic coagulating shears 1 cm to the left side of the falciform ligament. The branches of left portal vein, hepatic artery and hepatic vein on the cut surface were ligated with hem-o-loks and divided. The dilated bile duct was cut and stones were found. However, it was surprising that a large amount of mucin outflowed from the bile duct, which implied the intraductal mucinous tumor. The common bile duct (CBD) was explored, because it was dilated to 2 cm. There was no stone but massive mucin. After inserting a No.24 T-tube, the CBD and bile duct in the cut surface were closed with a continuous suture of 3-0 Vicryl. The specimens were removed through the enlarged subumbilical port (Figure ).
Resected specimens of intraductal papillary mucinous neoplasm-b (arrow head) and intraductal papillary mucinous neoplasm-p (arrow).
Intraoperative frozen section confirmed the mucinous tumors of the liver and pancreas and the resection margins were all negative. The operative time was 250 min and intraoperative bleeding was 150 mL. The postoperative course was uneventful, and the patient was discharged seven days later. She was followed up by abdominal CT six months later without signs of recurrence.
The gross finding was a 5 cm × 4 cm multiloculated cystic mass with stones in the left lateral lobe of liver, communicated with the dilated intrahepatic bile duct. Furthermore, multiple cystic lesions, ranging from 0.5 cm to 1.0 cm in diameter, were located in the pancreatic tail, in communication with the dilated pancreatic duct (Figure ). All cystic lesions, in both the liver and pancreas, were filled with gelatinous transparent mucin. Microscopically, the cystic wall consisted of hyperplastic fibrous tissues lined by high columnar mucous epithelium demonstrating papillary growth, but no ovarian-like stroma was identified (Figure ). There was no evidence of high-grade cellular dysplasia or stroma invasion suggesting malignancy in either specimen except for focal low-grade atypical hyperplasia of epithelium in the liver lesion. The immunohistochemical staining of the liver lesion showed cytokeratin 7 (CK7): +/-, CK20: +, cadual type homeobox transcription factor-2 (CDX-2): +, mucin core protein 1 (MUC1): -, mucin core protein 2 (MUC2): + and mucin core protein 5 (MUC5): +, while the pancreas lesion showed CK7: +, CK20: -, CDX-2: -, MUC1: +, MUC2: - and MUC5: +, which were subsequently categorized into pancreaticobiliary and intestinal subtypes of IPMN.
Histological features of intraductal papillary mucinous neoplasms by hematoxylin and eosin stain. A: Liver, × 20; B: Liver, × 100; C: Pancreas, × 40; D: Pancreas, × 200.