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From:
J Biol Chem. Author manuscript; available in PMC 2012 November 22.
Published in final edited form as:
J Biol Chem. 2006 February 24; 281(8): 4663–4670.
Published online 2005 December 22. doi: 10.1074/jbc.M508342200

FIGURE 5

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Late apoptotic cells induce the same signaling events as do early apoptotic cells

A, serum-starved BM macrophages were pre-exposed to increasing numbers of late apoptotic (Apo) BUMPT cells for 30 min and then stimulated with M-CSF for 15 min. Late apoptotic cells were added to macrophages at a cell to macrophage ratio ranging from 1:64 to 1:1 in 2-fold increments. B, serum-starved BM macrophages were pre-exposed to mixtures of apoptotic and necrotic (Nec) BUMPT cells for 30 min. The number of necrotic cells was kept constant at a necrotic cell to macrophage ratio of 1:1, and the number of apoptotic cells was increased as shown. Nonadherent cells were removed by rinsing, and macrophage lysates were probed with anti-active ERK1/2, p38, JNK, and p90Rsk antibodies. Equal loading was confirmed by Ponceau S staining of blotted proteins as well as probing for total ERK1/2, p38, JNK, or p90RSK (supplemental Fig. S5, except for total JNK, which is not shown). Apoptotic and necrotic cells alone contributed no active ERK1/2, p38, JNK, or p90Rsk in these experiments.

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