PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of capmcAbout manuscripts / A propos des manuscritsSubmit manuscript / soumettre un manuscrit
 
From:
J Biol Chem. Author manuscript; available in PMC Nov 22, 2012.
Published in final edited form as:
J Biol Chem. Feb 24, 2006; 281(8): 4663–4670.
Published online Dec 22, 2005. doi: 10.1074/jbc.M508342200
An external file that holds a picture, illustration, etc.
Object name is nihms2350f3.jpg Object name is nihms2350f3.jpg
FIGURE 3
Apoptotic cell activate p38 and JNK, whereas necrotic cells have no discernible effect on p38 and JNK
Serum-starved BM macrophages were exposed to increasing numbers of apoptotic (A) or necrotic (B) Jurkat cells for 15 min. Apoptotic or necrotic cells were added to macrophages at a cell to macrophage ratio ranging from 1:64 to 1:1 in 2-fold increments. Nonadherent cells were removed by rinsing, and macrophage lysates were probed with anti-active p38 or anti-active JNK antibody. Equal loading was confirmed by Ponceau S staining of blotted proteins as well as probing for total p38 (supplemental Fig. S3) or JNK (not shown). Apoptotic and necrotic cells alone contributed no active p38 and JNK in these experiments.