Search tips
Search criteria 


Logo of capmcAbout manuscripts / A propos des manuscritsSubmit manuscript / soumettre un manuscrit
J Biol Chem. Author manuscript; available in PMC 2012 November 22.
Published in final edited form as:
J Biol Chem. 2006 February 24; 281(8): 4663–4670.
Published online 2005 December 22. doi: 10.1074/jbc.M508342200


An external file that holds a picture, illustration, etc.
Object name is nihms2350f3.jpg
Apoptotic cell activate p38 and JNK, whereas necrotic cells have no discernible effect on p38 and JNK

Serum-starved BM macrophages were exposed to increasing numbers of apoptotic (A) or necrotic (B) Jurkat cells for 15 min. Apoptotic or necrotic cells were added to macrophages at a cell to macrophage ratio ranging from 1:64 to 1:1 in 2-fold increments. Nonadherent cells were removed by rinsing, and macrophage lysates were probed with anti-active p38 or anti-active JNK antibody. Equal loading was confirmed by Ponceau S staining of blotted proteins as well as probing for total p38 (supplemental Fig. S3) or JNK (not shown). Apoptotic and necrotic cells alone contributed no active p38 and JNK in these experiments.

Images in this article

  • FIGURE 1
  • FIGURE 2
  • FIGURE 3
  • FIGURE 4
  • FIGURE 5
  • FIGURE 6
  • FIGURE 7
Click on the image to see a larger version.