Apoptotic cell activate p38 and JNK, whereas necrotic cells have no discernible effect on p38 and JNK
Serum-starved BM macrophages were exposed to increasing numbers of apoptotic (A) or necrotic (B) Jurkat cells for 15 min. Apoptotic or necrotic cells were added to macrophages at a cell to macrophage ratio ranging from 1:64 to 1:1 in 2-fold increments. Nonadherent cells were removed by rinsing, and macrophage lysates were probed with anti-active p38 or anti-active JNK antibody. Equal loading was confirmed by Ponceau S staining of blotted proteins as well as probing for total p38 (supplemental Fig. S3) or JNK (not shown). Apoptotic and necrotic cells alone contributed no active p38 and JNK in these experiments.