PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of capmcAbout manuscripts / A propos des manuscritsSubmit manuscript / soumettre un manuscrit
 
From:
J Biol Chem. Author manuscript; available in PMC 2012 November 22.
Published in final edited form as:
J Biol Chem. 2006 February 24; 281(8): 4663–4670.
Published online 2005 December 22. doi: 10.1074/jbc.M508342200

FIGURE 1

An external file that holds a picture, illustration, etc.
Object name is nihms2350f1.jpg
Apoptotic cells inhibit whereas necrotic cells activate ERK1/2 in bone marrow-derived macrophages

Serum-starved BM-derived macrophages were stimulated with increasing numbers of apoptotic cells, necrotic cells, or latex beads alone for 15 min (A) or stimulated with M-CSF for 15 min following a 30-min pre-exposure to apoptotic cells, necrotic cells, or latex beads (B). Apoptotic or necrotic BUMPT cells were added to macrophages at a cell to macrophage ratio ranging from 1:64 to 1:1 in 2-fold increments, as indicated by the schematic diagram of increasing cell ratio. Nonadherent cells were removed by rinsing, and macrophage lysates were probed with anti-active ERK1/2 antibody. Equal loading was confirmed by Ponceau S staining of blotted proteins as well as probing for total ERK1/2 (supplemental Fig. S1). Apoptotic and necrotic cells alone contributed no active ERK1/2 in these experiments.

Images in this article

  • FIGURE 1
  • FIGURE 2
  • FIGURE 3
  • FIGURE 4
  • FIGURE 5
  • FIGURE 6
  • FIGURE 7
Click on the image to see a larger version.