miR-10a constrains TH17 differentiation in the presence of RA. (a) Naïve CD4+ T cells were transduced with a retroviral construct expressing a miR-10a-5p “sponge” target sequence (miR10a-5pT) or a scrambled sequence (control) and cultured under TH17 conditions with anti-IL-2 antibody and ATRA (1.5 nM). (b, c) Naïve CD4+ T cells were transduced with a retroviral construct expressing miR-10a or control vector under TH17 conditions and anti-IL-2 with (b) or without ATRA (1.5 nM) (c). Cells were analyzed for Foxp3 and IL-17A expressions by flow cytometry. A representative experiment is depicted in the left panel and pooled data from multiple independent experiments are shown in the right panels. Statistical significance was determined by paired sample t-test.