PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of bmcmudisBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Musculoskeletal Disorders
 
BMC Musculoskelet Disord. 2012; 13: 110.
Published online Jun 21, 2012. doi:  10.1186/1471-2474-13-110
PMCID: PMC3493365
Lipopolysaccharide treatment suppresses spontaneously developing ankylosing enthesopathy in B10.BR male mice: The potential role of interleukin-10
Jana Capkova,#1 Tomas Hrncir,corresponding author#2 Alena Kubatova,1 and Helena Tlaskalova-Hogenova2
1Laboratory of Diagnostics for Reproductive Medicine, Institute of Biotechnology AS CR, v.v.i., Videnska 1083, Prague 142 20, Czech Republic
2Department of Immunology and Gnotobiology, Institute of Microbiology AS CR, v.v.i., Doly 183, Novy Hradek 549 22, Czech Republic
corresponding authorCorresponding author.
#Contributed equally.
Jana Capkova: jana.capkova/at/img.cas.cz; Tomas Hrncir: tomas/at/gnotolab.com; Alena Kubatova: alena.kubatova/at/img.cas.cz; Helena Tlaskalova-Hogenova: tlaskalo/at/biomed.cas.cz
Received August 28, 2011; Accepted June 15, 2012.
Abstract
Background
Ankylosing enthesopathy (ANKENT) is an animal model of human ankylosing spondylitis. ANKENT is an inflammatory disease affecting the ankle and tarsal joints of the hind limbs in susceptible mouse strains. In the disease, the participation of intestinal microbiota components was suggested. Therefore, we attempted to increase the incidence of ANKENT by systemic administration of lipopolysaccharide (LPS), which is a component of bacterial cellular walls and stimulates inflammatory processes.
Methods
ANKENT occurrence, serum cytokine profiles, spleen cellular composition and in vitro cytokine response to LPS were analysed in LPS-treated and control LPS-untreated B10.BR male mice.
Results
Contrary to expectations, LPS treatment decreased the incidence of ANKENT in LPS-treated group compared to control LPS-untreated group. Flow cytometry analysis of splenocytes showed an increased percentage of macrophages, dendritic cells and neutrophils and a decreased percentage of B cells, T cells and T helper cells in LPS-treated males following LPS administration. In addition, LPS-treated males had significantly elevated IL-6 and IL-10 serum levels. At 20–22 weeks after the final LPS application, splenocytes from LPS-treated mice were more susceptible to in vitro LPS stimulation than those of the controls and produced significantly higher levels of TNFα and IL-6.
Conclusions
Repeated systemic stimulation with microbial component lipopolysaccharide in early adulthood significantly reduced the incidence of ANKENT in B10.BR mice and this finding can support the “hygiene hypothesis”. In LPS-treated mice, the innate immunity parameters and the level of anti-inflammatory IL-10 cytokine were significantly increased. Nevertheless, the immunological mechanism of the LPS protective effect remains unclear.
Articles from BMC Musculoskeletal Disorders are provided here courtesy of
BioMed Central