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Logo of bmcmicrBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Microbiology
 
BMC Microbiol. 2012; 12: 234.
Published online Oct 16, 2012. doi:  10.1186/1471-2180-12-234
PMCID: PMC3493300
Antibiotic producing microorganisms from River Wiwi, Lake Bosomtwe and the Gulf of Guinea at Doakor Sea Beach, Ghana
Adelaide A Tawiah,1 Stephen Y Gbedema,corresponding author1 Francis Adu,1 Vivian E Boamah,1 and Kofi Annan2
1Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and technology, Kumasi, Ghana
2Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and technology, Kumasi, Ghana
corresponding authorCorresponding author.
Adelaide A Tawiah: giftadel/at/yahoo.com; Stephen Y Gbedema: sgbedema.pharm/at/knust.edu.gh; Francis Adu: franceadu.pharm/at/knust.edu.gh; Vivian E Boamah: etsiapa/at/yahoo.com; Kofi Annan: annankofi/at/yahoo.com
Received February 20, 2012; Accepted September 27, 2012.
Abstract
Background
Microorganisms have provided a wealth of metabolites with interesting activities such as antimicrobial, antiviral and anticancer. In this study, a total of 119 aquatic microbial isolates from 30 samples (taken from water bodies in Ghana) were screened by the agar-well diffusion method for ability to produce antibacterial-metabolites.
Results
Antibacterial activity was exhibited by 27 of the isolates (14 bacteria, 9 actinomycetes and 4 fungi) against at least one of the indicator microorganisms: Enterococcus faecalis (ATCC 29212), Bacillus thuringiensis (ATCC 13838), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), Proteus vulgaris (NCTC 4635) and Bacillus Subtilis (NCTC 10073). A sea isolate MAI2 (identified as a strain of Pseudomonas aeruginosa) exhibited the highest antibacterial activity (lowest zone of inhibition = 22 mm). The metabolites of MAI2 extracted with chloroform were stable to heat and gave minimum inhibitory concentrations ranging between 250 and 2000 μg/ml. Bioautography of the extract revealed seven active components.
Conclusion
This study has therefore uncovered the potential of water bodies in the West African sub-region as reservoirs of potent bioactive metabolite producing microorganisms.
Keywords: Aquatic microorganisms, Antibiotics, Ghana, Multi-drug resistance
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