PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of bmcpsBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Plant Biology
 
BMC Plant Biol. 2012; 12: 156.
Published online Aug 31, 2012. doi:  10.1186/1471-2229-12-156
PMCID: PMC3492168
Production of engineered long-life and male sterile Pelargonium plants
Begoña García-Sogo,1 Benito Pineda,1 Edelín Roque,1 Teresa Antón,1 Alejandro Atarés,1 Marisé Borja,2,3 José Pío Beltrán,1 Vicente Moreno,1 and Luis Antonio Cañascorresponding author1
1Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Ciudad Politécnica de la Innovación, Edf. 8E. C/Ingeniero Fausto Elio s/n, Valencia E-46011, Spain
2BIOMIVA S.L, Carretera M-511 Km. 2, Villaviciosa de Odón Madrid, E-28670, Spain
3Plant Response Biotech S.L. Parque Científico-Tecnológico Montegancedo, Pozuelo de Alarcón, Madrid, E-28223, Spain
corresponding authorCorresponding author.
Begoña García-Sogo: bgarcia/at/btc.upv.es; Benito Pineda: bpineda/at/btc.upv.es; Edelín Roque: edroque/at/ibmcp.upv.es; Teresa Antón: maanmar2/at/doctor.upv.es; Alejandro Atarés: aatares/at/ibmcp.upv.es; Marisé Borja: marise.borja/at/plantresponse.com; José Pío Beltrán: jbeltran/at/ibmcp.upv.es; Vicente Moreno: vmoreno/at/ibmcp.upv.es; Luis Antonio Cañas: lcanas/at/ibmcp.upv.es
Received March 29, 2012; Accepted August 2, 2012.
Abstract
Background
Pelargonium is one of the most popular garden plants in the world. Moreover, it has a considerable economic importance in the ornamental plant market. Conventional cross-breeding strategies have generated a range of cultivars with excellent traits. However, gene transfer via Agrobacterium tumefaciens could be a helpful tool to further improve Pelargonium by enabling the introduction of new genes/traits. We report a simple and reliable protocol for the genetic transformation of Pelargonium spp. and the production of engineered long-life and male sterile Pelargonium zonale plants, using the pSAG12::ipt and PsEND1::barnase chimaeric genes respectively.
Results
The pSAG12::ipt transgenic plants showed delayed leaf senescence, increased branching and reduced internodal length, as compared to control plants. Leaves and flowers of the pSAG12::ipt plants were reduced in size and displayed a more intense coloration. In the transgenic lines carrying the PsEND1::barnase construct no pollen grains were observed in the modified anther structures, which developed instead of normal anthers. The locules of sterile anthers collapsed 3–4 days prior to floral anthesis and, in most cases, the undeveloped anther tissues underwent necrosis.
Conclusion
The chimaeric construct pSAG12::ipt can be useful in Pelargonium spp. to delay the senescence process and to modify plant architecture. In addition, the use of engineered male sterile plants would be especially useful to produce environmentally friendly transgenic plants carrying new traits by preventing gene flow between the genetically modified ornamentals and related plant species. These characteristics could be of interest, from a commercial point of view, both for pelargonium producers and consumers.
Keywords: Pelargonium zonale, Pelargonium peltatum, pSAG12 promoter, ipt gene, Engineered long-lived plants, Delayed senescence, Engineered male sterility, PsEND1 promoter, Barnase, Anther ablation, Biosafe ornamentals
Articles from BMC Plant Biology are provided here courtesy of
BioMed Central