Dehalogenimonas spp. can Reductively Dehalogenate High Concentrations of 1,2-Dichloroethane, 1,2-Dichloropropane, and 1,1,2-Trichloroethane

doi:10.1186/2191-0855-2-54

AMB Express. 2012; 2: 54.

Published online 2012 October 9. doi: 10.1186/2191-0855-2-54

PMCID: PMC3492069

Dehalogenimonas spp. can Reductively Dehalogenate High Concentrations of 1,2-Dichloroethane, 1,2-Dichloropropane, and 1,1,2-Trichloroethane

Andrew D Maness,¹ Kimberly S Bowman,^1,² Jun Yan,^1,⁴ Fred A Rainey,^2,³ and William M Moe¹

¹Department of Civil and Environmental Engineering, Louisiana State University, 3513B Patrick Taylor Hall, Baton Rouge, LA, 70803, USA

²Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA

³Department of Biological Sciences, University of Alaska Anchorage, Anchorage, AK, 99508, USA

⁴Present address: Jun Yan, Department of Microbiology and Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN, USA

Corresponding author.

Andrew D Maness: amanes1/at/gmail.com; Kimberly S Bowman: kbowma3/at/tigers.lsu.edu; Jun Yan: jyan5/at/utk.edu; Fred A Rainey: farainey/at/uaa.alaska.edu; William M Moe: moemwil/at/lsu.edu

Received October 1, 2012; Accepted October 2, 2012.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The contaminant concentrations over which type strains of the species Dehalogenimonas alkenigignens and Dehalogenimonas lykanthroporepellens were able to reductively dechlorinate 1,2-dichloroethane (1,2-DCA), 1,2-dichloropropane (1,2-DCP), and 1,1,2-trichloroethane (1,1,2-TCA) were evaluated. Although initially isolated from an environment with much lower halogenated solvent concentrations, D. alkenigignens IP3-3^T was found to reductively dehalogenate chlorinated alkanes at concentrations comparable to D. lykanthroporepellens BL-DC-9^T. Both species dechlorinated 1,2-DCA, 1,2-DCP, and 1,1,2-TCA present at initial concentrations at least as high as 8.7, 4.0, and 3.5 mM, respectively. The ability of Dehalogenimonas spp. to carry out anaerobic reductive dechlorination even in the presence of high concentrations of chlorinated aliphatic alkanes has important implications for remediation of contaminated soil and groundwater.

Keywords: Bioremediation, Chlorinated alkanes, Dehalogenimonas, Reductive dechlorination, Dehalogenation

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