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Diurnal rhythms of putative circadian clock genes in Nicotiana attenuata . Expression of orthologs of the circadian clock genes were first examined using our time course microarray data (accession number GSE30287). Wild-type N. attenuata plants were harvested every 4h for one day from leaves and roots. After RNA isolation, each sample was hybridized on Agilent single color technology arrays designed from the N. attenuata transcriptome (accession number GPL13527). Mean (± SE) levels of transcript abundance of (A) NaLHY, (B) NaTOC1, and (C) NaZTL in leaves (n=3) and roots (n=3) at each harvest time. Gray boxes depict the dark period of LD (16h light/ 8h dark). Na, Nicotiana attenuata; LHY, late elongated hypocotyl; TOC1, timing of cab expression 1; ZTL, zeitlupe.