Diurnal rhythms of putative circadian clock genes in Nicotiana attenuata .
Expression of orthologs of the circadian clock genes were first examined using our time course microarray data (accession number GSE30287). Wild-type N. attenuata
plants were harvested every 4
h for one day from leaves and roots. After RNA isolation, each sample was hybridized on Agilent single color technology arrays designed from the N. attenuata
transcriptome (accession number GPL13527). Mean (± SE) levels of transcript abundance of (A
, and (C
in leaves (n
3) and roots (n
3) at each harvest time. Gray boxes depict the dark period of LD (16
h light/ 8
h dark). Na, Nicotiana attenuata
; LHY, late elongated hypocotyl; TOC1, timing of cab expression 1; ZTL, zeitlupe.
Articles from BMC Plant Biology are provided here courtesy of