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Logo of bmcvetresBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Veterinary Research
 
BMC Vet Res. 2012; 8: 42.
Published online Apr 2, 2012. doi:  10.1186/1746-6148-8-42
PMCID: PMC3489715
Transmissibility of caprine scrapie in ovine transgenic mice
Katherine I O’Rourke,corresponding author1 David A Schneider,1 Terry R Spraker,2 Rohana P Dassanayake,3 Margaret A Highland,3 Dongyue Zhuang,1 and Thomas C Truscott1
1United States Department of Agriculture, Agricultural Research Service, Pullman, WA, 99164, USA
2Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, 80526, USA
3Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164, USA
corresponding authorCorresponding author.
Katherine I O’Rourke: korourke/at/vetmed.wsu.edu; David A Schneider: das/at/vetmed.wsu.edu; Terry R Spraker: terry.spraker/at/colostate.edu; Rohana P Dassanayake: rohana1/at/vetmed.wsu.edu; Margaret A Highland: mah/at/vetmed.wsu.edu; Dongyue Zhuang: Dongyue/at/vetmed.wsu.edu; Thomas C Truscott: ttruscot/at/vetmed.wsu.edu
Received September 13, 2011; Accepted February 17, 2012.
Abstract
Background
The United States control program for classical ovine scrapie is based in part on the finding that infection is typically spread through exposure to shed placentas from infected ewes. Transmission from goats to sheep is less well described. A suitable rodent model for examining the effect of caprine scrapie isolates in the ovine host will be useful in the ovine scrapie eradication effort. In this study, we describe the incubation time, brain lesion profile, glycoform pattern and PrPSc distribution patterns in a well characterized transgenic mouse line (Tg338) expressing the ovine VRQ prion allele, following inoculation with brain from scrapie infected goats.
Results
First passage incubation times of caprine tissue in Tg338 ovinized mice varied widely but second passage intervals were shorter and consistent. Vacuolation profiles, glycoform patterns and paraffin-embedded tissue blots from terminally ill second passage mice derived from sheep or goat inocula were similar. Proteinase K digestion products of murine tissue were slightly smaller than the original ruminant inocula, a finding consistent with passage of several ovine strains in previous reports.
Conclusions
These findings demonstrate that Tg338 mice propagate prions of caprine origin and provide a suitable baseline for examination of samples identified in the expanded US caprine scrapie surveillance program.
Keywords: Prion, Mouse, Transgenic, Caprine
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